• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권2호
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• pp.135-140
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• 2008

Loss of E-cadherin (E-cad) expression has been found in multiple cancers and is postulated to facilitate tumor cell dissociation and metastais. Promotor methylation may provides an alternative pathway for loss of gene function. This study evaluated the role of hypermethylation in the down-regulation of E-cad in oral squamous cell carcinoma (OSCC). We examined the E-cad expression by immunohistochemical staining and detected methylation status by methylation-specific polymerase chain reaction (MSP) in 20 OSCC tissues. Overally, 12 (60%) cases of hypermethylation of E-cad were detected and we found there were no correlation between methylation and age, histologic grade, lympn node metastasis, tumor size and clinical stage. However, Eleven (73.3%) of 15 samples which was negative for E-cad staining showed hypermethylation of E-cad promotor region. On the other hand, only one (20%) of 5 E-cad positive sample was observed with methylated status. The underexpression of E-cad was found to be related to promotor hypermethylation (p=0.035). In conclusion, we suggest that hypermethylation play a role in inactivation of E-cad gene and may be a appreciable biomarker for diagnosis and treatment of OSCC.

• Journal of Microbiology
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• 제39권2호
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• pp.109-115
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• 2001

Salmonella typhimurium possesses a cad operon, which contributes to an adaptive response against an acidifying environment. In Escherichia coli, the activation of the cad operon is dependent on cadC, which is located upstream of the operon. However, the activator of cad operon in S. typhimurium has not been known until now. In this study, we selected a putative cadC mutant by trasposon mutagenesis and cloned the cadc of S. typhimurium. Moreover, the cadC mutant was complemented by cadC clone. The cadC gene from S. typhimurium LT-2 consists of 1539 bp encoding a polypeptide ob 512 amino acids, and shows sequence similarity to cadC of E. coli with 53% identity and 67% similarity. The hydrophobicity profile of th S. typhimurim CadC sequence is very similar to E. coli CadC.

• 한국전자거래학회:학술대회논문집
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• 한국전자거래학회 2003년도 종합학술대회 논문집
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• pp.39-44
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• 2003

ㆍ 분산 환경에서 설계와 생산이 이루어짐 ㆍ 서로 다른 CAD 시스템 상호간에 오류 없는 CAD 정보 교환이 필수 그러나 현실은 CAD정보 교환 과정에서 많은 오류를 발생하고, 후공정에서 이러한 오류를 파악하고 수정하는데, 많은 시간과 비용이 투입되고 있다.(중략)

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2221-2224
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• 2014

Objective: To explore the relationship between expressions of cell adhesion molecules CD44 v6 and E-cadherin (E-cad) and lymphatic metastasis in non-small cell lung cancer (NSCLC). Materials and Methods: Eightyseven tissue samples obtained from patients with primary NSCLC were collected in our hospital from Dec., 2007 to Dec., 2012, and the expressions of CD44 v6 and E-cad gene proteins in these samples were detected by immunohistochemical method. Results: In the tissue without lymphatic metastasis, the positive expression rate of CD44 v6 was significantly lower, whereas the normal expression rate of E-cad was notably higher than that with lymphatic metastasis (55.6% vs. 78.4%, 47.2% vs. 21.6%), and both differences had statistical significance (P<0.05). Besides, CD44 v6 and E-cad expressions had a significant correlation in the NSCLC tissue with lymphatic metastasis (P<0.05). Conclusions: The positive expression of CD44 v6 and abnormal expression of E-cad may play a very important role in promoting lymphatic metastasis of NSCLC, with synergistic effect. Hence, detection of CD44 v6 and E-cad expressions is conductive to judging the lymphatic metastasis in NSCLC.

• 한국치위생학회지
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• 제16권5호
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• pp.695-700
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• 2016

Objectives: The purpose of the study was to investigate the effect of CAD/CAM ceramic thickness on shade. Methods: 24 disk-shaped ceramic specimens (E.Max CAD & Empress CAD, $12mm{\times}12mm$) were prepared and divided into 4 groups (n=6). Ceramic specimens in 2 thicknesses (0.8 mm, 1.0 mm) were made low translucency(LT) shade A2. All specimens were measured as L*, a*, b* using spectrophotometer. The color differences(${\Delta}E$ values) between the specimen and a control target block ($12{\times}12{\times}1.2mm$) were calculated. Two-way ANOVA performed between 2 groups(material and thickness). Results: As the thickness increased, the $L^*$ (for all groups) and $b^*$ value (for all groups) increased and the $a^*$ value(for LR group) decreased. The mean color differences caused by thickness was acceptible (${\Delta}E$ > 2) for group LD1. But the mean color differences by thickness were not selected (${\Delta}E$ < 2) for group LD2, LR1, LR2. Conclusions: The different thickness can influence the final color of CAD/CAM ceramic.

• 치위생과학회지
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• 제11권4호
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• pp.299-303
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• 2011

The purpose of this case report was to present an example of an esthetic and functional rehabilitation of anterior teeth with tetracycline discoloration and minor morphological abnormality of a 39-year old female. A chairside computer-aided design/computer-aided manufacturing (CAD/CAM) system with CEREC AC was applied for the prosthetic procedure and all ceramic crowns made with lithum disilicate (IPS e.max CAD) restored the esthetic and functional features of sixteen anterior teeth successfully.

• International Journal of CAD/CAM
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• 제4권1호
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• pp.1-10
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• 2004

Through reviewing and comparing the current virtual shopping malls web sites integrated VR into E-commerce, this paper analyzed both the advantages and disadvantages of two kinds of methods for product presentation: 2D image based and 3D model based presentation method. Using the virtual shopping mall (EasyMall) as a showcase, we presented the architecture of the system and the development technologies, especially those in the mixed presentation method. The presentation and customization methods in the two related modules, including the PhoneShow for mobile phone and EasyShow for textile products, were discussed. It indicated that the integration of E-commerce with VR could provide consumers with virtual experience and intelligent service for business activities. Furthermore, the product presentation methods can be made available for use in different cases.

• 한국전자거래학회지
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• 제9권4호
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• pp.117-136
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• 2004

치열한 경쟁 환경 하에서 빠른 시간 안에 고객이 원하는 제품을 생산해야 하는 즉, 시장 출시까지의 시간 (time to market) 단축의 필요성이 더욱 높아짐에 따라, 제품 설계 시에 협업(collaborative) 설계와 편집(configuration) 설계의 중요성이 커지고 있다. 이를 위해서 효율적인 부품 검색 방법이 필요하고, 그 방법의 하나로써 전자 카달로그 (e-Catalog)가 많이 사용된다. 편집설계를 통한 설계 모델 재사용을 위해서는 파라메트릭 정보가 필수적이다. 따라서 전자 카달로그는 파라메트릭 (parametric) 정보가 포함된 CAD 모델 라이브러리를 제공해야 한다. 그러나 제품의 구성에 따라 무수히 많은 조합이 존재하기 때문에, 기존의 방법으로는 파라메트릭 CAD 모델 라이브러리를 구축하기가 어렵다. 예를 들어, 몰드베이스 종이 카달로그의 한 페이지에는 8000만개 이상의 조합이 존재한다. 이와 같은 문제를 해결하기 위해서, 본 논문에서는 파라메트릭 CAD 모델 자동 생성을 위한 테이블 파라메트릭 (table parametric) 방법을 제안하고, 테이블 파라메트릭 모델을 선정하는 방법과 설계 파라메트릭 세트를 구성하는 방법에 대해서 설명한다. 테이블 파라메트릭 방법을 적용하게 되면, 전자 카달로그 시스템의 분류체계와 테이블 파라메트릭 모델의 설계 파라메터 세트 (design parameters set) 와의 매핑을 통해, 모든 조합의 몰드베이스 CAD 모델을 자동으로 생성할 수 있다.

• 미생물학회지
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• 제37권4호
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• pp.259-264
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• 2001

S. typhimurium cadBA operon의 발현에는 산성 pH와 고농도의 lysine 등 적어도 두 가지의 세포외부 신호가 요구된다. pH와 lysine신호에 따른 cadBA 발현 조절 기작을 이해하기 위하여, Tn10삽입, 자발적 돌연변이, EMS돌연변이 등을 수행하여 JF2238과 cadA-lacZ의 발현특성에 차이를 보이는 돌연변이체를 선별하였다. cadBA의 양성적 조절자의 유전자인 cadC내의 돌연변이 중 cadC4 돌연변이는 pH-비의존성, lysine-의존성 발현을, cadC6 돌연변이는 pH-비의존성, lysine-비의존성 cadA-lacZ 발현을 나타내었다. 산성 pH, lysine이 없는 조건에서 cadA-lacZ 발현을 유도하는 cadR::Tn10과 cadR3 돌연변이체를 분리하여 cadR이 lysine이 없는 조건에서 cadBA 발현을 음성적으로 조절하는 음성적 조절자의 유전자임을 알 수 있었다. cadR 돌연변이체는 lysine의 독성 유사체인 thiosine에 대해 내성($Ts^{r}$)을 나타냈으며, E. coli의 lysP 클론을 갖고 있는 pLYSP에 의해 돌연변이 형질이 보상되었다. 또한 lysine decarboxylase (CadA)의 반응산물인 cadaverine은 cadC+ 균주에서 cadA-lacZ 발현에 억제효과를 나타냈으나, cadC 돌연변이에는 억제효과가 나타나지 않았다.

• 대한치과기공학회지
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• 제38권3호
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• pp.165-173
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• 2016

Purpose: This study was conducted to examine the color stability of temporary prosthetic materials for dental CAD-CAM. Method: 2 kinds of existing acrylic resin temporary filling materials and 2 kinds of polymer prosthetic materials for dental CAD-CAM were used for testing, and five specimens respectively were absorbed in soy sauce, red pepper paste, and coffee. A Chroma Meter was used as analysis equipment. The color change of the specimens was observed to calculate ${\Delta}E$ value throughout 2 days, 3 days, 4 days, and 5 days and this was analyzed and the following result was obtained. Results: Snap's ${\Delta}E$ value was bigger than Vipi block(p<0.05). Red pepper paste had the largest impact on the color change of resin(${\Delta}E$), followed by soy sauce and coffee(p<0.000). With the passage of time, ${\Delta}E$ value was inversely(-) related and decreased somewhat(p<0.000).