Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Cloning and Sequencing Analysis of cadC Encoding Transcriptional Activator CadC from Salmonella typhimurium

  • Kim, Bae-Hoon (Graduate School of Biotechnology, Korea University) ;
  • Lee, Ho-Jeong (Graduate School of Biotechnology, Korea University) ;
  • Lee, In-Soo (Department of Microbilolgy, University) ;
  • Bang, Sung-Ho (Department of Biology, Hanseo University) ;
  • Kim, Joon (Graduate School of Biotechnology, Korea University) ;
  • Park, Yong-Keun (Graduate School of Biotechnology, Korea University)
  • Published : 2001.06.01
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Abstract

Salmonella typhimurium possesses a cad operon, which contributes to an adaptive response against an acidifying environment. In Escherichia coli, the activation of the cad operon is dependent on cadC, which is located upstream of the operon. However, the activator of cad operon in S. typhimurium has not been known until now. In this study, we selected a putative cadC mutant by trasposon mutagenesis and cloned the cadc of S. typhimurium. Moreover, the cadC mutant was complemented by cadC clone. The cadC gene from S. typhimurium LT-2 consists of 1539 bp encoding a polypeptide ob 512 amino acids, and shows sequence similarity to cadC of E. coli with 53% identity and 67% similarity. The hydrophobicity profile of th S. typhimurim CadC sequence is very similar to E. coli CadC.

Keywords

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