• Korean Journal of Microbiology
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• v.55 no.3
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• pp.181-190
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• 2019

In Escherichia coli, DicA protein is involved in cell division control. DicA protein is known to bind DNA better at $25^{\circ}C$ than at $37^{\circ}C$. However, the molecular cause of the temperature dependent binding is not clear. In this study, we investigated how DicA binds DNA and why its DNA binding activity depends on temperature. An unique in vivo DNA binding assay developed in this laboratory showed that unlike the homologous proteins such as RovA or SlyA, DicA uses its N-terminal domain for DNA binding. The in vivo DNA binding assay of DicA also demonstrated that the temperature-dependent DNA binding activity does not come from Cnu or H-NS that is known to bind DNA better at $25^{\circ}C$ than at $37^{\circ}C$. Electrophoretic Mobility Shift Assay (EMSA), when performed with purified DicA protein, did not show temperature-dependent DicA binding activity. However when EMSA was performed with crude protein from WT E. coli cells, temperature-dependent DicA binding activity was observed, suggesting that there is a factor(s) that confers temperature DNA binding activity of DicA in vivo.

• Atmosphere
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• v.31 no.2
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• pp.241-249
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• 2021

A smart city utilizes data collected from various sensors through the internet of things (IoT) and improves city operations across the urban area. Recently substantial research is underway to examine all aspects of data that requires for the smart city operation. Atmospheric data are an essential component for successful smart city implementation, including Urban Air Mobility (UAM), infrastructure planning, safety and convenience, and traffic management. Unfortunately, the current level of conventional atmospheric data does not meet the needs of the new city concept. New and innovative approaches to developing high spatiotemporal resolution of observational and modeling data, resolving the complex urban structure, are expected to support the future needs. The geographic information system (GIS) integrates the atmospheric data with the urban structure and offers information system enhancement. In this study we proposed the necessity and applicability of the high resolution urban meteorological dataset based on heavy fog cases in the smart city region (e.g., Sejong and Pusan) in Korea.

• Applied Chemistry for Engineering
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• v.22 no.5
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• pp.551-554
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• 2011

One of the key issues in the research of organic field-effect transistors (OFETs) is the low-voltage operation. To address this issue, we synthesized poly(styrene-r-benzocyclobutene-r-methyl methacrylate) (P(S-r-BCB-r-MMA)) as a thermally cross-linkable gate dielectrics. The P(S-r-BCB-r-MMA) showed high quality dielectric properties due to the negligible volume change during the cross-linking. The pentacene FETs based on the 34 nm-thick P(S-r-BCB-r-MMA) gate dielectrics operate below 5 V. The P(S-r-BCB-r-MMA) gate dielectrics yielded high device performance, i.e. a field-effect mobility of $0.25cm^2/Vs$, a threshold voltage of -2 V, an sub-threshold slope of 400 mV/decade, and an on/off current ratio of ${\sim}10^5$. The thermally cross-linkable P(S-r-BCB-r-MMA) will provide an attractive candidate for solution-processable gate dielectrics for low-voltage OFETs.

• Maritime Security
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• v.1 no.1
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• pp.311-343
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• 2020

Because of the global warming, the Arctic Ocean is expected to be ice-free by the year 2035. When the Arctic Ocean will be opened, a number of national interests will become more salient as experiencing a shortened sailing distance and decreasing navigation expense, possibility of natural resources transport by sea from Arctic Circle, and indirect-profit making by building a herb port in Asia. To secure the national interests and support the free activities of people in this region, R.O.K government is trying to make advanced policies. In order to carry out the naval tasks in the Arctic Ocean, using the operational characteristics(mobility, flexibility, sustainability, presence of capabilities, projection) is necessary. To this end, ROK Navy should analyze the operational environment (O.E.) by its capability(weakness and strength), opportunity, and threat. R.O.K. Navy should make an effort over the following issues to implement the tasks in the Arctic Ocean: first, Navy needs to map out her own plan (Roadmap) under the direction of government policies and makes crews participate in the education·training programs in home and abroad for future polar experts. Third, to develop the forces and materials for the tasks in cold, far operations area, Navy should use domestic well-experienced shipbuilding skills and techniques of the fourth industrial revolution. Next, improving the combined operations capabilities and military trust with other countries in the Arctic region to cover the large area with lack of forces' number and to resolve the ports of call issues. Lastly, preparation in advance to execute a variety of missions against military and non-traditional threats such as epidemics, HA/DR, SOLAS, in the future operation area is required.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.134-148
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• 2021

Background: Lung cancer has a high incidence worldwide, and most lung cancer-associated deaths are attributable to cancer metastasis. Although several medicinal properties of Panax ginseng Meyer have been reported, the effect of ginsenosides Rk1 and Rg5 on epithelial-mesenchymal transition (EMT) stimulated by transforming growth factor beta 1 (TGF-β1) and self-renewal in A549 cells is relatively unknown. Methods: We treated TGF-β1 or alternatively Rk1 and Rg5 in A549 cells. We used western blot analysis, real-time polymerase chain reaction (qPCR), wound healing assay, Matrigel invasion assay, and anoikis assays to determine the effect of Rk1 and Rg5 on TGF-mediated EMT in lung cancer cell. In addition, we performed tumorsphere formation assays and real-time PCR to evaluate the stem-like properties. Results: EMT is induced by TGF-β1 in A549 cells causing the development of cancer stem-like features. Expression of E-cadherin, an epithelial marker, decreased and an increase in vimentin expression was noted. Cell mobility, invasiveness, and anoikis resistance were enhanced with TGF-β1 treatment. In addition, the expression of stem cell markers, CD44, and CD133, was also increased. Treatment with Rk1 and Rg5 suppressed EMT by TGF-β1 and the development of stemness in a dose-dependent manner. Additionally, Rk1 and Rg5 markedly suppressed TGF-β1-induced metalloproteinase-2/9 (MMP2/9) activity, and activation of Smad2/3 and nuclear factor kappa B/extra-cellular signal regulated kinases (NF-kB/ERK) pathways in lung cancer cells. Conclusions: Rk1 and Rg5 regulate the EMT inducing TGF-β1 by suppressing the Smad and NF-κB/ERK pathways (non-Smad pathway).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.3
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• pp.417-425
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• 2022

Interest in micro cars is growing around the world, and policy support for micro cars has been increasing in Korea. It is important to meet minimum safety standards for the operation of micro cars on roads due to concerns around micro car safety and the limited driving range of micro cars. In this study, visibility experiments that included safety identification of micro cars were conducted to try and prevent a decrease in driver reaction time. Safety identification lights were installed to the rear of a micro car, and the visibility and discomfort of the vehicle were evaluated to determine whether the micro car was safe to drive on an expressway. As a result, the installation effect of Micro car which install safety identification lamp was found when joining the point at an acceleration lane of the grade separation intersection, and that light on/off could be effective when entering an expressway. If the micro car operation plan proposed in this study is applied, the safety of micro cars on expressways can be increased by improving the visibility of micro car.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.21 no.5
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• pp.28-41
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• 2022

A road system with CV(Connected Vehicle)s, which is often referred to as a cooperative intelligent transportation system (C-ITS), provides various road information to drivers using an in-vehicle warning system. Road environments with CVs induce drivers to reduce their speed or change lanes to avoid potential risks downstream. Such avoidance maneuvers can be considered to improve driving behaviors from a traffic safety point of view. Thus, empirically evaluating how a given in-vehicle warning information affects driving behaviors, and monitoring of the correlation between them are essential tasks for traffic operators. To quantitatively evaluate the effect of in-vehicle warning information, this study develops a method to calculate compliance rate of drivers where two groups of speed profile before and after road information is provided are compared. In addition, conventional indexes (e.g., jerk and acceleration noise) to measure comfort of passengers are examined. Empirical tests are conducted by using PVD (Probe Vehicle Data) and DTG (Digital Tacho Graph) data to verify the individual effects of warning information based on C-ITS constructed in Seoul metropolitan area in South Korea. The results in this study shows that drivers tend to decelerate their speed as a response to the in-vehicle warning information. Meanwhile, the in-vehicle warning information helps drivers to improve the safety and comport of passengers.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.8 no.2
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• pp.89-102
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• 2018

With the development of mobile technology, asset management on the Internet have also developed a lot. Drawing on Technology Acceptance Model, this study examines YUEBAO deployment to model consumers' purchase intention to use financial products offered online. In this study, we hypothesized that the characteristics of online asset management product will affect the purchase intention through perceived usefulness and conduct empirical analysis on Chinese consumers. In the study model, the independent variables were considered to include individual involvement, experience, product protection, corporate credibility, convenience, mobility, and familiarity. In addition, the parameters constitute the usefulness, and the dependent variable is the purchase. The results are as follows. First, YUEBAO's complementarity, corporate credibility, convenience, and familiarity have a significant influence on YUEBAO's usefulness. Second, The YUEBAO's usefulness has a noticeable effect on the purchase intention. To perceive the high usefulness, the practicality strategy of enhancing the protection property, corporate reliability, convenience and familiarity of the online asset management product is needed. The study of consumer purchase behavior and consumer purchase intention of online wealth management products is very valuable for academic and practical work.

• Composites Research
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• v.36 no.5
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• pp.349-354
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• 2023

Poly(3-hexylthiophene) (P3HT) is a conjugated polymer that is highly soluble in organic solvents and is readily available. However, its electrical properties as an active channel in electronic devices are not enough for practical applications, necessitating further improvement in the properties. In this study, we demonstrate that the blending of two P3HT polymers (i.e., regio-regular (RR) P3HT and regio-random (RRa) P3HT) with different regioregularities can significantly improve charge transport characteristics of the blend films. The morphological and electrical properties of the blend films were systematically investigated by varying the ratio between two P3HT polymers. Atomic force microscopy (AFM), X-ray diffraction (XRD), and UV-visible absorption spectroscopy (UV-vis) were employed to evaluate the morphological and optoelectronic properties of the blend films. The crystallinity of the blend films increased with increasing the content of RRa-P3HT to 20 wt% and gradually decreased as the content increased to 80%. Consistently, the highest charge carrier mobility was obtained from the blend films containing 20 wt% RRa-P3HT, which value was measured to be 0.029 cm²/V·s. The values gradually decreased to 0.0007 cm²/V·s with increasing the content of RRa-P3HT to 80 wt%.

• Journal of Advanced Navigation Technology
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• v.27 no.6
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• pp.690-698
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• 2023

Information sharing and decision making between airport stakeholders became possible after the introduction of airport cooperative making system (A-CDM). This also resulted in optimizing aircraft handling time and increased the efficiency of aircraft operations. Technological advances have recently led to the development of urban air mobility (UAM) which is a small aircraft taking off and landing vertically. It is emerging as a new air transportation system in the future due to its advantage of saving time and solving congestion problem in the urban area. This study aims to suggest how vertiport cooperative decision making system (V-CDM) should be managed for efficient operation of UAM. By establishing procedure for decision making system based on Vertiport ecosystem of UAM. By establishing procedure for decision making system based on Vertiport ecosystem and UAM aircraft, unnecessary flight delays or cancellations can be minimized and efficiency of UAM operation will be improved as well.