• 대한수의학회지
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• 제27권1호
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• pp.61-68
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• 1987

This study was conducted to determine the microorganisms inhabitating in cow genitalia and their antimicrobial drug susceptibility. During the period between July, 1985 and February, 1986, a total of 111 cow genitalia, 58 from Korean native and 53 from dairy cow, were sampled at three abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital samples. In addition antimicrobial drug susceptibility test for the microorganisms isolated, some synergistic activity among drugs were examined on the major organism isolated from the cases of endometritis and pyometra. The results are summerized as follows: 1. Among the bacteria isolated from the genitalia, Staphylococcus spp., C. pyogenes, E. coli, Proteus spp., Streptococcus spp., Bacillus spp. were most frequently isolated whereas the genera of Pasteurella, Pseudomonas, Klebsiella and Yersina were detected far less frequently. 2. In Korean native cow the genera of Straphylococcus and Steptococcus were more isolated than dairy cow while in dairy cow the genera of Corynebacterium, Proteus, Escherichia were more of ten isolated than Korean native cow. 3. From cow genital organs showing lesions of endometritis and prometra, C. pyogenes was most frequently isolated, the isolation rate being 60 percent, and follow by Staphylococcus spp., Proteus spp., E. coli and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility test conducted on the major organisms isolated showed that all the isolates were susceptible to gentamicin, cephalosporin and sulfisoxazole, but resistant to tetracycline and penicillin. 5. Twenty-nine isolates of C. pyogenes were submitted to the synergistic activity test of cephalosporin, kanamycin and streptomycin with penicillin. Synergists were demonstrated in 90 percent, 31 percent and 27 percent of isolates examined by the combined use of penicillin and cephalosporin, penicillin and kanamycin, penicillin and streptomycin, respectively. About 10 percent of the isolates were found to be indifferent by the synergism test.

• Structural Engineering and Mechanics
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• 제50권1호
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• pp.89-103
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• 2014

According to experimental studies made so far, design formula of shear characteristics suggested by ISO 22762 and JEAG 4614, representative design code for Lead Rubber Bearing(LRB) shows dependence caused by changes in compressive stress. Especially, in the case of atypical special structure, such as a nuclear power structure, placement of seismic isolation bearing is more limited compared to that of existing structures and design compressive stress is various in sizes. As a result, there is a difference between design factor and real behavior with regards to shear characteristics of base isolation device, depending on compressive stress. In this study, a full-scale low hardness device of LRB, representative base isolation device was manufactured, analyzed, and then evaluated through an experiment on shear characteristics related to various compressive stresses. With design compressive stress of the full-scale LRB (13MPa) being a basis, changes in shear characteristics were analyzed for compressive stress of 5 MPa, 10 MPa, 13 MPa, 15 MPa, and 20 MPa based on characteristics test specified by ISO 22762:2010 and based on the test result, a regression analysis was made to offer an empirical formula. With application of proposed design formula which reflected the existing design formula and empirical formula, trend of horizontal characteristics was analyzed.

• Journal of Power Electronics
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• 제13권5호
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• pp.746-756
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• 2013

The modularized subunit of an electronic power transformer (EPT) is a series connection of two H-bridge voltage-source converters and a DC-DC converter with a high-frequency isolation transformer (HFIT). On the basis of cascading and paralleling the modularized subunits, EPT can be used in high-voltage and large-current applications in the power system. This paper discusses the steady state analysis of the modularized subunit of EPT. Theoretical analysis considers the influences of the two H-bridge voltage-source converters on the two sides of the DC-DC converter. We deduce the formulas of the theoretical calculation on the internal electrical characteristics of EPT (e.g., the voltages of the DC-bus capacitor and the primary side peak current of the HFIT). This paper provides guidance on the design and selection of EPT key elements (e.g., the DC-bus capacitors and HFIT). Experimental results are obtained from a single subunit of a laboratory model rated at 962 V, 15 kVA. All calculations, simulations, and experiments confirm the theoretical analysis of the subunit of EPT.

• 한국임상수의학회지
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• 제17권2호
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• pp.316-320
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• 2000

The present study conducted to evaluate the isolation frequency of Salmonella sap., Escherichia O157:H7 and Listeria monocytogenes in fecal samples collected form 33 dairy cow feedlots in West Gyeong-nam province from tan. 179\ulcorner to Feb. 1999. Salmonella spp. were isolated from 8.7% of fecal samples and 57.5% of dairy cow feedlots participaiting in this study had at least one positive fecal 7ample. The isolation rates of Salmonella spp. in each herd size were 42.9% in ln less than 125 heads, 63.6% in 25% to 50 heads and 75% in more than 50 heads. Of the positive sample, the most common serogroup recovered was Bl(31.5%), followed by C1 and D(22.9%), C2(16.2%), A(4.2%) and E(2.1%), in order. However, E. coli O157:H7 and Listeria monocytogenes were net detected in the fecal samples collected from all feedlots.

• 한국추진공학회지
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• 제20권2호
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• pp.56-66
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• 2016

모델기반 FDI 과정에서 모델오차와 센서잡음은 피할 수 없으므로 견실성은 모델기반 FDI에서 매우 중요하다. 본 연구에서는 이러한 선형모델 오차 및 신호잡음으로 인하여 고장진단 과정에서 발생하는 결함판단 오류들을 비선형 NARX (Nonlinear Auto Regressive eXogenous) 모델과 칼만추정기를 적용하여 개선하는 방법을 제안하였다. 최종 고장판단은 퍼지로직을 이용하여 발생하는 오차의 추이에 대한 확률로 결정하여 순간적인 신호잡음에 강인하도록 설계하였다. 시뮬레이션을 통하여 운용 환경조건에서 엔진제어기의 고장허용에 따른 성능을 확인하였다.

• 한국군사과학기술학회지
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• 제15권2호
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• pp.111-120
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• 2012

Built-In-Test is a design feature in more and more advanced weapon system. During development test and evaluation(DT&E) it is critical that the BIT system be evaluated. The BIT system is an integral part of the weapon system and subsystem. Built-In-Test assists in conducting on system and subsystem failure detection and isolation to the Line Replaceable Unit(LRU). This capability reduces the need for highly skilled personnel and special test equipment at organizational level, and reduces maintenance down-time of system by shortening Total Corrective Maintenance Time. During DT&E of weapon system the objective of BIT system evaluation is to determine BIT capabilities achieved and to identify deficiencies in the BIT system. As a result corrective actions are implemented while the system is still in development. Through the use of the reiterative BIT evaluation the BIT system design was corrected, improved, or updated, as the BIT system matured.

• 대한수의학회지
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• 제28권1호
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• pp.67-73
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• 1988

This study was conducted to isolate etiological agents from the 103 scouring piglets in Gyeongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical served. The incidence of scouring piglet was most prevalent as 81.6% in the age of 2 to 4 weeks after birth, while the rate was less than 10% in the age of 5 to 6 weeks and under 1 week after birch. When compared the isolation frequency of the each etiological agent, enteropathogenic E. coli was most prevalent as 46.6%, thermophilic Campylobacter 26.2% and Salmonellae was 8.7% in order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85 (20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni were belong to type I (50.0%) and II (25.0%), and most strains of C. coli were belong to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C. and each 1 strain was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, wherease about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C.jejuni and C. coli were sensitive to kanamycin and gentamicin.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.153-160
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• 2010

The present study was carried out for the isolation of bacteriocin-producing enterococci from indigenous sources. Gram-positive enterococci are known for having the ability to produce enterocins with good antimicrobial potential. A total of 34 strains were isolated from processed dairy products of Pakistan and seven out of them were found to be member of genus Enterococcus on selective enumeration. Biochemical and molecular characterization revealed that four of these isolates (IJ-03, IJ-07, IJ-11, and IJ-12) were Enterococcus faecalis and three (IJ-06, IJ-21, and IJ-31) were Enterococcus faecium. Local processed cheese was the source of all enterococcal isolates, except E. faecium IJ-21 and IJ-31, which were isolated from indigenous yoghurt and butter samples, respectively. Bacterial isolates were sensitive to commonly used antibiotics except methicillin and kanamycin. They also lacked critical virulence determinants, mainly cytolysin (cyl), gelatinase (gel), enterococcal surface protein (esp), and vancomycin resistance (vanA and vanB). Polymerase chain reaction amplification identified that enterocin A and P genes were present in the genome of E. faecium IJ-06 and IJ-21, whereas the E. faecium IJ-31 genome showed only enterocin P genes. No amplification was observed for genes that corresponded with the enterocins 31, AS-48, L50A, and L50B, and ent 1071A and 1071B. There were no signals of amplification found for E. faecalis IJ-11, indicating that the antimicrobial activity was because of an enterocin different from those checked by PCR. Hence, the indigenous bacterial isolates have great potential for bacteriocin production and they had antibacterial activity against a variety of closely related species.

• 한국미생물·생명공학회지
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• 제16권5호
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• pp.379-383
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• 1988

활성슬러지법에 의한 벤젠폐수 처리실험을 통하여 활성슬러지의 벤젠처리중에 실제 그에 관여하는 세균을 분리 동정하였다. 반응조에서 분리한 세균은 6종으로, Pseudomonas fluorescens, Enterobacter agglomerans, Enterobacter cloacae, Klebsiella oxytoca, Citrobacter freundii와 Klebsiella pneumoniae로 동정되었다. 벤젠 분해능을 측정한 결과는 P. fluorescenss가 55%를 18시간만에 처리했고, E. cloacae는 24%, K. oxytoca 는 32%, E. agglomerans는 41%의 분해능을 보였다. P. fluorescens의 증식 최적조건은 온도 25-31$^{\circ}C$와 pH 7.0으로 확인되었다. P. fluorescens가 우점종일때 나타나는 지로생물은 원생동물인 Aspidisca sp.였다. 벤젠 농도가 387mg/$\ell$일 때에는 Aspidisca sp.와 Litonotus sp.가 증식을 최대로 하였고, 벤젠 농도가 1,600mg/$\ell$에서는 Litonotus sp.와 Vorticella sp.는 거의 성장하지 않았다. Glucose 배지에서는 모두 성장율이 높았다.

• 대한수의학회지
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• 제35권3호
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• pp.531-536
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• 1995

Salmonella species are the most prevalent etiologic agents of food-borne acute gastroenteritis. Direct isolation of bacteria from the contaminated food, stool and animal tissues has been used for the diagnosis of salmonellosis routinely. However, isolation of bacteria is time consuming work and not so highly sensitive. In recent years, improved methods of polymerization chain reaction(PCR) and probe hybridization technique have led to the developement of diagnostic assays which employ to detect various human and animal pathogenic bacteria. In this study, we have performed the polymerization chain reaction to detect Salmonella pullorum from tissues and stool samples of chickens with two specific primers, ST5 and ST8C. The target DNA fragment of PhoE gene was successfully amplified from liver, spleen, pancreas, heart, lung, ovary, oviduct and feces samples. The amplified DNA fragments were hybridized with Salmonella typhymurium TA3000 PhoE probe by Southern hybridization. The PCR to amplify the PhoE gene was highly rapid and sensitive method to detect Salmonella pullorum from tissues and stool samples.