• Parasites, Hosts and Diseases
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• v.53 no.2
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• pp.147-154
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• 2015

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it should be properly monitored by an inhibition control. In this study, a cloning vector harboring a modified target DNA sequence (${\approx}375bp$) was constructed to be used as a competitive internal amplification control (IAC) for a conventional PCR assay that detects ${\approx}550bp$ of the Cryptosporidium oocyst wall protein (COWP) gene sequence in human feces. Modification of the native PCR target was carried out using a new approach comprising inverse PCR and restriction digestion techniques. IAC was included in the assay, with the estimated optimum concentration of 1 fg per reaction, as duplex PCR. When applied on fecal samples spiked with variable oocysts counts, ${\approx}2$ oocysts were theoretically enough for detection. When applied on 25 Cryptosporidium-positive fecal samples of various infection intensities, both targets were clearly detected with minimal competition noticed in 2-3 samples. Importantly, both the analytical and the diagnostic sensitivities of the PCR assay were not altered with integration of IAC into the reactions. When tried on 180 randomly collected fecal samples, 159 were Cryptosporidium-negatives. Although the native target DNA was absent, the IAC amplicon was obviously detected on gel of all the Cryptosporidium-negative samples. These results imply that running of the diagnostic PCR, inspired with the previously developed DNA extraction protocol and the constructed IAC, represents a useful tool for Cryptosporidium detection in human feces.

• Journal of Korean Tunnelling and Underground Space Association
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• v.14 no.2
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• pp.141-157
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• 2012

For the application TBM tunneling method, Both tunnel design standard and case study designed & constructed in domestic and foreign have been conducted. According to the study, the number of lane and inner shape (single or duplex) vary depending on the volume of traffic. Also extra space located in the top and bottom of tunnel is used for a multipurpose such as ventilation, disaster prevention, maintenance and administration. To find area ratio according to the components of road TBM tunnel, a standard section was considered as a two-lane road. Then, the analysis of area ratio of this section which consists of components for clearance, extra space in upper and lower tunnel was carried on two widths of shoulder. In addition, after a structural analysis, a thickness requirement of lower slab which is essential for road tunnel was derived on a few supporting types. Through correlation analysis, the ranges of available cross-sectional area between slab thickness and lower extra space of the tunnel was presented.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.38 no.5 s.118
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• pp.47-53
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• 2006

To meet the ever increasing quality demand of coated papers and duplex boards, the local gloss variation and print unevenness must be avoid. To do this, surface roughening phenomenon which affects local gloss and print unevenness was investigated. In this study, the effect of roughness and densification of sheet obtained at various calendering conditions on surface roughening was examined. To evaluate the calendering effect properly we introduced a new value of roughening index by moisture for evaluation of surface roughening. Caliper and roughness of sheet decreased with increasing of calendering temperature and pressure, and the remarkable reduction of those properties occurred at the temperature around Tg. Roughening index increased when the amount of water increased and calendaring temperature and pressure increased. And as the sheet became denser by calendering, the roughening index increased to some extent. But roughening index by moisture is the lowest for sheet precalendered at higher temperature condition. When the surface of calendered sheet is rougher, there is more roughening of surface. The severe condition of calendering made the roughening index by moisture small.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.4
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• pp.696-706
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• 2007

Multiprocessor system on chip (MPSoC) platform has set a new innovative trend for the System on Chip (SoC) design. With the rapidly approaching billion transistors era, some of the main problem in deep sub-micron technologies characterized by gate lengths in the range of 60-90 nm will arise from non scalable wire delays, errors in signal integrity and un-synchronized communication. These problems may be addressed by the use of Network on Chip (NOC) architecture for future SoC. Most future SoCs will use network architecture and a packet based communication protocol for on chip communication. This paper presents an adaptive wormhole routing with proactive turn prohibition to guarantee deadlock free on chip communication for NOC architecture. It shows a simple muting architecture with five full-duplex, flit-wide communication channels. We provide simulation results for message latency and compare results with those of dimension ordered techniques operating at the same link rates.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.11
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• pp.2021-2026
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• 2007

In the parer, we design a non-coherent UWB system by adopting the architecture of a simplified asynchronous transmission and the edge-triggered pulse transmission, which makes e system performance independent of the share of the transmitted waveform, robust to multipath channels. The designed non-coherent UWB transceiver architecture has an advantage of the simple realization since any mixer, high-speed correlator, and high-sampling A/D converter are not necessary at the cost of performance degradation of about 3dB. Further, the designed non-coherent UWB transceiver is actually implemented with the wireless CANVAS prototype testbed in short range indoor application environments such as a lecture room. The implemented prototype testbed is proven to offer the data rate of 115kbps on the conditions of Peer-to-Peer(P-to-P) in the indoor channel within the range of about 10m.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.6
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• pp.984-989
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• 2008

In this paper, we will design a 800MHz broadband antenna after a problem of the narrow bandwidth is improved. This multiple band antenna unifies the CDMA(Code Division Multiple Access), GSM(Global System for Mobile Telecommunication) and TRS(Trunked Radio System) band in the UHF band, and then it is possible at the shore base station or repeater as the commercial use. It used the duplex resonance effect it had the L-shared feeding structure which adds the U-slot. And it improved profit using stack structure. It was measured that the frequency bandwidth of the designed antenna which is planed $792{\sim}1040MHz$ with 248MHz(33%). And the antenna gain is 9.4dBi, 3dB beam width $60^{\circ}$ in radiation pattern.

• Bulletin of the Korean Chemical Society
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• v.12 no.5
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• pp.554-559
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• 1991

The photophysical and photochemical properties of khellin were compared with those of 8-methoxypsoralen (8-MOP). Quantum yields of fluorescence and triplet formation decreases as solvent polarity increases, which is opposite to 8-MOP, and photocycloadditivity of khellin to olefins is much lower than that of 8-MOP. Electron ejection from khellin by laser flash was not observed, but observed from 8-MOP. As models of 4',5'-monoadducts of khellin or 8-MOP with thymine base, khellin<>dimethylfumarate 4',5'-monoadduct (KDF) was also compared with 8-MOP<>thymidine 4',5'-monoadduct (F-2) in those properties to give some insight on the second-step biadduct formation resulting in cross-links of DNA duplex. KDF and F-2 were very similar to khellin and 8-MOP in photophysical properties, respectively. However, KDF did not form adducts with various olefins, and thus it is thought that 2,3-double bond of chromone moiety in khellin is hardly reactive in contrast with 3,4-double bond of coumarin moiety in 8-MOP. These results indicate that khellin is fairly photostable compound, a poor type Ⅰ photodynamic sensitizer and producer of ${O_2}^{-}$ which is some cause of phototoxic erythemal reactions and undesirable side effects. Therefore khellin is safer to use than 8-MOP in photochemotherapy of some skin diseases. Although khellin is much less reactive than 8-MOP, khellin must be also a monofunctional drug. Since khellin is, however, as effective as 8-MOP in photochemotherapy of some skin diseases, it is suggested that khellin may be different from 8-MOP in the action mechanism.

• Steel and Composite Structures
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• v.33 no.1
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• pp.37-66
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• 2019

In cold-formed steel structures, such as trusses, wall frames and portal frames, the use of back-to-back built-up cold-formed stainless-steel lipped channels as compression members are becoming increasingly popular. The advantages of using stainless-steel as structural members are corrosion resistance and durability, compared with carbon steel. The AISI/ASCE Standard, SEI/ASCE-8-02 and AS/NZS do not include the design of stainless-steel built-up channels and very few experimental tests or finite element analyses have been reported in the literature for such back-to back cold-formed stainless-steel channels. Current guidance by the American Iron and Steel Institute (AISI) and the Australian and New Zealand (gAS/NZS) standards for built-up carbon steel sections only describe a modified slenderness approach, to consider the spacing of the intermediate fasteners. Thus, this paper presents a numerical investigation on the behavior of back-to-back cold-formed stainless-steel built-up lipped channels. Three different grades of stainless steel i.e., duplex EN1.4462, ferritic EN1.4003 and austenitic EN1.4404 have been considered. Effect of screw spacing on the axial strength of such built-up channels was investigated. As expected, most of the short and intermediate columns failed by either local-global or local-distortional buckling interactions, whereas the long columns, failed by global buckling. All three grades of stainless-steel stub columns failed by local buckling. A comprehensive parametric study was then carried out covering a wide range of slenderness and different cross-sectional geometries to assess the performance of the current design guidelines by AISI and AS/NZS. In total, 647 finite element models were analyzed. From the results of the parametric study, it was found that the AISI & AS/NZS are conservative by around 10 to 20% for cold-formed stainless-steel built-up lipped channels failed through overall buckling, irrespective of the stainless-steel grades. However, the AISI and AS/NZS can be un-conservative by around 6% for all three grades of stainless-steel built-up channels, which failed by local buckling.

• Korean Journal of Materials Research
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• v.31 no.9
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• pp.519-524
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• 2021

The impact properties of two austenitic Fe-23Mn-0.4C steels with different Al contents for cryogenic applications are investigated in this study. The 4Al steel consists mostly of austenite single-phase microstructure, while the 5Al steel exhibits a two-phase microstructure of austenite and delta-ferrite with coarse and elongated grains. Charpy impact test results reveal that the 5Al steel with duplex phases of austenite and delta-ferrite exhibits a ductile-to-brittle transition behavior, while the 4Al steel with only single-phase austenite has higher absorbed energy over 100 J at -196 ℃. The SEM fractographs of Charpy impact specimens show that the 4Al steel has a ductile dimple fracture regardless of test temperature, whereas the 5Al steel fractured at -100 ℃ and -196 ℃ exhibits a mixed fracture mode of both ductile and brittle fractures. Additionally, quasi-cleavage fracture caused by crack propagation of delta-ferrite phase is found in some regions of the brittle fracture surface of the 5Al steel. Based on these results, the delta-ferrite phase hardly has a significant effect on absorbed energy at room-temperature, but it significantly deteriorates low-temperature toughness by acting as the main site of the propagation of brittle cracks at cryogenic-temperatures.

• Journal of IKEEE
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• v.25 no.3
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• pp.420-424
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• 2021

The massive MIMO system, which is a core technology of 5G communication systems, has a problem that it is difficult to implement in a frequency division duplex system based on limited channel feedback because a large amount of channel information is required at the transmitting end. In order to solve this problem, the Joint Spatial Division and Multiplexing (JSDM) technique that dramatically reduces the channel information requirement by removing interference between the user groups using channel correlation information that does not change for a long time has been proposed. Recently, a regularized JSDM technique has been proposed to further improve performance by allowing residual interference between the user groups. However, such JSDM-related studies were mainly designed to focus on inter-group interference cancellation, and thus performance analysis was not performed in a more realistic environment assuming limited feedback in the intra-group interference cancellation phase. In this paper, we analyze the performance of the JSDM and regularized JSDM techniques according to the number of groups and users in a limited feedback environment, and through the simulation results, demonstrate that the regularized JSDM technique shows a more remarkable advantage compared to the existing JSDM in a limited feedback environments.