• 생약학회지
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• 제45권3호
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• pp.268-274
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• 2014

The aim of this study is to explore the potent phosphodiesterase 4 and 7 inhibitor from various herbal medicines for atopy treatment. In this study, 51 kinds of each herbal medicine, which were extracted with ethanol, was carried out the screening of PDE 4 and 7 inhibition using enzyme inhibitory assay. Of these, 8 species of herbal medicines, Rubus coreanus, Duchesnea chrysantha, Alisma orientale, Rehmannia glutinosa, Angelica dahurica, Thuja orientalis, Astragalus membranaceus and Perilla frutescens were screened as potential inhibitor against PDE 4 and 7. Among 8 species, Duchesnea chrysantha showed poteinial anti-atopic effect on DNCB-induced atopic model. Duchesnea chrysantha extract decreased serum IgE and histamine release significantly.

• 생약학회지
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• 제43권3호
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• pp.201-205
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• 2012

In the course of screening pancreatic lipase inhibitory activity, total methanolic extract and EtOAc-soluble fraction of Duchesnea chrysantha showed significant inhibitory activity. Further fractionation and isolation of the EtOAc-soluble fraction resulted in five compounds, which were identified as trans-tiliroside (1), isovitexin (2), kaempferol-8-O-${\beta}$-glucoside (3), kaempferol-3-O-${\beta}$-glucoside (4) and quercetin-3-O-${\beta}$-glucoside (5). All the five flavonoids derivatives were first reported from this plant but showed weak inhibitory effects on pancreatic lipase activity.

• 대한의생명과학회지
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• 제19권1호
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• pp.48-54
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• 2013

Atopic dermatitis is a recurrent or chronic eczematous skin disease with severe pruritus and has annually increased in Korea. In this study, we investigated whether Duchesnea chrysantha (Dc) extracts have an anti-inflammatory effect in human monocytic THP-1 cells and human eosinophilic EoL-1 cells. The dried and powdered whole plants of Dc were extracted with 80% EtOH (Dc-1). The residue was diluted with water, and then successively partitioned with n-hexane, EtOAc, and BuOH to produce the n-hexane (Dc-2), EtOAc (Dc-3), BuOH (Dc-4), and the water-soluble fractions (Dc-5), respectively. The mite extract and LPS increased the production of IL-6, IL-8 and MCP-1 in THP-1 cells and the increase was strongly suppressed by Dc-3 extract, as compare with other extracts. Dc-3 also inhibited the release of IL-6 increased by mite extract and LPS in EoL-1 cells. However, Dc-3 extract increased IL-8 production induced by the mite extract and LPS in EoL-1 cells. These results suggest that Dc extract may be used as anti-inflammatory agents in treating allergic disorders such as asthma and atopic dermatitis.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.476-479
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• 1994

Five pohenolic compounds were isolated from 80% aq. acetone extract of Duchesnea chyrysantha. Their crytotoxicities were screened by the colorimetric tetrazolium assay (MIT assay). Gallic acid, methyl caffeate, protocatechuic acid and pedunculagin mildly inhibited the survival of $PC_{14}{\;}and{\;}MKN_{45}$ human cancer cell. Brevifolin carboxylic acid showed a strong cytotoxic activity.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.280.1-280
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• 2000

No Abstract, See Full Text

• 동의생리병리학회지
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• 제26권6호
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• pp.902-907
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• 2012

The worldwide prevalence and severity of allergic diseases including atopic dermatitis and asthma has increased dramatically over the past decade, especially in developed countries. Mast cells are important effector cells in allergic reactions. The purpose of this study was undertaken to investigate the anti-allergic activities of the extract of Duchesnea chrysantha (DCE). DCE was prepared by extracting with 80% ethanol. In the present study, we investigate the effect of DCE on the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$, IL-6, IL-8, and histamine in the human mast cell line (HMC-1 cells) and on the scratching behavior in hairless mice. Various concentrations of DCE were treated before the activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. PMA plus A23187 significantly increased TNF-${\alpha}$, IL-$1{\beta}$, IL-6, and IL-8 production compared with media control. We also show that the increased cytokines such as TNF-${\alpha}$ IL-$1{\beta}$, IL-6, and IL-8 were significantly inhibited by DCE in a dose-dependent manner. Moreover, DCE inhibited the histamine release from HMC-1 cells stimulated by compound 48/80, which promotes histamine release. Futhermore, the administration of DCE reduced the scratching behavior induced by pruritogen (compound 48/80 or histamine) in hairless mice. These results suggest that DCE has a potential use as a medicinal plant for treatment against allergy-related disease.

• 동의생리병리학회지
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• 제26권1호
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• pp.59-66
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• 2012

Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.

• 생명과학회지
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• 제28권1호
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• pp.83-89
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• 2018

본 연구에서는 고지방 고콜레스테롤 식이 흰쥐에서 뱀딸기풀 파우더가 간조직의 항산화작용에 미치는 영향을 관찰하였다. 실험군을 4군으로 나누어 정상 식이군(N 군), 고지방 고콜레스테롤 식이군(HF 군), 고지방 고콜레스테롤 식이에 뱀딸기풀 파우더를 5% 첨가한 군(DA 군), 고지방 고콜레스테롤 식이에 뱀딸기풀 파우더를 10% 첨가한 군(DB 군)으로 나누었다. 식이 및 식수는 자유섭취하게 하였으며 4주간 사육한 후 희생시켰다. 간 조직 중의 항산화 효소계인 GSH-px 및 catalase 활성은 정상군(N 군)에 비해 HF 군에서는 유의적으로 감소되었으나 HF 군에 비해 뱀딸기풀 파우더 공급군(DA 및 DB 군)에서는 유의적으로 증가되었다. 유리기를 소거하는 지표인 간조직의 superoxide radical 함량을 microsome에서 측정한 결과 정상군에 비해 HF 군에서 유의적인 증가를 나타내었으나 뱀딸기풀 공급으로 유의하게 감소되었다. 또한 mitochondria에서는 HF 군에 비해 DB 군에서 정상군 수준으로 감소되어졌다. 간조직의 cytosol에서 $H_2O_2$의 함량에서는 DA 및 DB 군 모두에서 유의적인 감소로 정상군 수준을 나타내었다. 간조직의 microsome 및 mitochondria에서의 산화단백질 함량은 정상군에 비해 HF 군에서 유의적으로 증가 되었으나 뱀딸기풀 파우더의 공급으로 유의적으로 감소하였다. 과산화지질의 함량을 간조직에서 관찰 한 결과, 정상군에 비해 HF 군에서 유의적으로 증가하였고, 또한 고지방 고콜레스테롤 식이로 인해 증가된 과산화지질은 뱀딸기풀 파우더를 공급한 군에서 감소를 나타내었으며, 특히 10% 공급한 군에서 유의한 결과를 나타내었다. 이러한 결과로 미루어 뱀딸기풀에 함유된 여러 항산화성분들은 효과적으로 활성산소종을 소거하여 고지방 고콜레스테롤 식이로 인해 유발된 산화적 손상을 효과적으로 완화시켰을 것이라 사료되어진다.

• 한국유기농업학회지
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• 제21권1호
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• pp.105-113
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• 2013

Antibacterial activity is an important feature for the development of antibiotics alternatives. Plant extract is considered as a promising alternative for organic farming. In this study, a total of 11 plants were extracted using ethanol to determine their antibacterial activities against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum. The synergistic interaction among plant extracts was also investigated. Plants used in this study were Carthamus nctoricus L. (pA), Poncirus trifollata Raf. (pB), Scutellaria balcalensis Georgi (pC) Prunus sargentii (pD), Cucurbita moschata $D_{UCH}$ Leaf (pE), Allium cepa L. peel (pF) Portulaca oleracea L. (pG), Xanthium strumarium L. (pH), Duchesnea chrysantha (pI), Cudrania tricuspidata (pJ) and Juniperus chinensis L. (pK). The pB and pA had the most broad antibacterial spectrum and the highest activity against to Staph. aureus among plant extract, respectively. In the synergistic interaction, the mixtures of pA and pC as well as pA and pF had batter antibacterial activity against to Staph. Aureus compared with other mixtures.

• Preventive Nutrition and Food Science
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• 제22권3호
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• pp.203-210
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• 2017

The antioxidant and antimicrobial activities of three kinds of strawberry ethanol extracts from Robus corchorifolius L. f. (RCL), Rubus parvifolius L. var. parvifolius (RPL), and Duchesnea chrysantha Miq. (DCM) were investigated. The RPL was highest (P<0.05) in phenolic, flavonoid, and anthocyanin contents. 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of RPL and DCM extracts were higher than that of RCL (P<0.05). Hydrogen peroxide scavenging activity of RPL was high compared to DCM and RCL (P<0.05). RCL exhibited a significant (P<0.05) potent antioxidant activity in nitric oxide radical inhibition. Inhibition diameter zone (nearest mm) of extracts against the test bacteria ranged from 11.5 in RCL to 12.5 in DCM against Staphylococcus aureus, from 10.5 in RCL to 13.5 in DCM against Streptococcus pneumoniae, from 8.5 in DCM to 10.5 in RCL against Escherichia coli, and the same inhibition of 10 mm in three of the extracts against Klebsiella pneumoniae. However, there was no inhibition against fungi Aspergillus niger and Candida albicans. Three of the extracts had the same minimum inhibitory concentration values of 12.50, 12.50, and $6.25{\mu}g/mL$ against S. aureus, K. pneumoniae, and S. pneumoniae, respectively. On the other hand, MIC values of 12.50, 12.50, and $6.50{\mu}g/mL$ were recorded for RPL, DCM, and RCL against E. coli, respectively. The result of present study revealed that extracts from three kinds of strawberries could be potential candidates as antioxidant and antimicrobial sources for functional food industries.