• Title/Summary/Keyword: Dual infection

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A pathological study in subsequent infection of PRRS virus to pigs with porcine pleuropneumoniae (돼지생식기호흡기증후군과 돼지 흉막폐렴의 복합감염에 따른 병리학적 연구)

  • Jeong, Hyun-Kyu;Han, Jeong-Hee
    • Korean Journal of Veterinary Pathology
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    • v.7 no.1
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    • pp.27-41
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    • 2003
  • The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A. pleuropneumonia in pigs. Twenty three 7-weeks-old commercial pigs were infected with PRRS virus and/or A. pleuropneumoniae serotype 5 intratracheally. Feed conversion, clincal signs, gross and histopathological lesions and immunohistochemical findings were examined. 1. Feed conversion ratio in dual-infected pigs with PRRS virus and A. pleuropneumoniae were higher than that of single- infected pigs with PRRS virus or A. pleuropneumoniae. 2. Dual-infected pigs with PRRS virus followed by A. pleuropneumoniae showed more severe clinical signs and gross, histopathological and immunohistochemical pulmonary lesions. The results indicated that dual infections with PRRS virus and A. pleuropneumoniae caused more severe respiratory lesions and growth retardation in pigs than single infection with PRRS virus or A. pleuropneumoniae.

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Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets - II. Clinical sign, histopathological lesion and immunohistochemical finding - (포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 - II. 임상증상, 병리조직학적 검사 및 면역조직학적 검사 -)

  • Jin, Wen;Kim, Yeong-Hun;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.189-200
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    • 2009
  • The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. In clinical signs, the signs observed in dual-infected with PEDV and PCV2 piglets and alone infected with PEDV piglets ranged from diarrhoea to vomiting and dehydration. Dual-infected piglets developed signs of anorexia, vomiting and watery diarrhoea within 12 hpi. Nevertheless alone -infected piglets caused pasty diarrhea at first. In mortality, dual infections showed 25%, but alone -infections showed 8.3%, respectively. In gross findings, piglets dual-infected with PEDV and PCV2 appeared the severe findings of congestion, distension of lumen, milder curdes of undigested milk in stomach than those of single-infected piglets. In histopathological findings, piglets of dual-infection group appeared the more severe findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. Significant (P<0.05) decrease in VH:CD ratio in dually infected piglets compared to piglets from alone-PEDV infections. In immunohistochemical findings, strong hybridization signals in dual-infected piglets observed moderate to severe villous atrophy or vacuolation with positive cells arranged continuously over the villi. In the lumen, exfoliated enterocytes were strongly positive in dual-infected piglets. A number of PEDV-positive cells in dual-infected pigs were significantly higher than that in alone PEDV-infected piglets.

Seed Transmission Rates of Bean pod mottle virus and Soybean mosaic virus in Soybean May Be Affected by Mixed Infection or Expression of the Kunitz Trypsin Inhibitor

  • Nam, Moon;Bae, Hanhong;Hammond, John;Domier, Leslie L.;Youn, Young-Nam;Lee, Bong-Choon;Lim, Hyoun-Sub
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.114-117
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    • 2013
  • To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mosaic virus (SMV) enhanced the seed transmission rate of BPMV in one experiment. In contrast, the rate of SMV seed transmission was not affected by BPMV co-infection. In a second preliminary study, the rate of SMV transmission was lower in an isoline of Williams 82 that contained a null mutation for the Kunitz trypsin inhibitor gene than in Williams 82. In this preliminary study, we observed that factors such as protease inhibitor expression and dual infection may affect the frequency of seed transmission of BPMV and SMV.

Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets - I. Serological result, FA test and RT-PCR - (포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 - I. 혈청학적 결과, 형광항체검사 및 RT-PCR 검사 -)

  • Jin, Wen;Kim, Yeong-Hun;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.177-187
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    • 2009
  • The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. The results obtained were as follows: Antibodies against PCV2 and PEDV were detected at 24, 36, 48, 60 and 72h postinfection. And the antibody titers of alone infections with PEDV were gradually reduced and increased from 60 hpi to 72 hpi. Whereas, the antibody titers of dual infections with PCV2 and PEDV were gradually reduced all the time. PEDV antigens were detected at 24, 36, 48, 60 and 72 hpi, being seen almost exclusively in feces and small intestines from PEDV-infected piglets and PCV2-coinfected piglets. The detection rate of PEDV in feces and jejunum tissues by RT-PCR were 94.9% and 91.1% in dual infections and 87.1% and 83.6% in alone infections with PEDV, respectively. In dual infected piglets, significantly more PEDV antigens were detected in the feces and small intestines tissues at 24 hpi (P<0.05) than in the same feces and tissues of the alone infected piglets. Thereafter, at 72 hpi significantly more PEDV antigens (P<0.05) was detected in the jejunal tissues of the dual infected piglets with than of alone PEDV-infected piglets. The detection rate of PEDV antigen in the duodenum, jejunum and ileum by IFA were 91.3%, 91.3% and 83.3% in dual infected piglets and 75.0%, 83% and 75% in alone infected piglets, respectively. Intense and specific fluorescence signals were more often seen within jejunal villous enterocytes in dual infected piglets than alone infected piglets.

A dual-plane approach for surgical treatment of pseudoaneurysm with arteriovenous fistula in hemodialysis patients

  • Kim, Ji Min;Tak, Min Sung;Kang, Jin Seok;Moon, Chul
    • Archives of Plastic Surgery
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    • v.48 no.3
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    • pp.287-292
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    • 2021
  • Background We report the efficacy of a dual-plane approach using a Dufourmentel skin flap with a purse-string suture of the de-epithelized dermis to manage pseudoaneurysm at the vascular access site for hemodialysis. Methods A retrospective analysis was conducted of 61 patients from 2013 to 2018 with pseudoaneurysms at the arteriovenous fistula or graft who were treated with rhomboid excision, vessel repair with a purse-string suture, and a full-thickness Dufourmentel skin flap. The success rate was defined as the probability of complete wound closure and intact vascular access patency without infection or other complications. Results The success rate was 93.4% at 6 months postoperatively. Complications included newly occurring pseudoaneurysms (n=2), wound dehiscence (n=1) and bleeding (n=1). There were no complications such as stenosis or thrombosis from the procedure. Conclusions A dual-plane approach using a Dufourmentel skin flap with a purse-string suture for vessel repair was shown to be a favorable option for managing stable, small (diameter <2 cm) pseudoaneurysms without infection, rapid expansion, or patency issues of the vascular access.

INDUCTION OF SYSTEMIC RESISTANCE IN CUCUMBER AGAINST ANTHRACNOSE BY PLANT GROWTH PROMOTING FUNGI

  • Hyakumachi, Mitsuro
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1997.06a
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    • pp.47-55
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    • 1997
  • Plant growth promoting fungi(PGPF) obtained from zoysiagrass rhizosphere offer dual advantages - induse systemic disease resistance response in cucumber to C. orbiculare infection and cause enhancement of plant growth and increase yield. PGPF protected plants either by colonizing roots or by their metabolites. PGPF offer an advantage by protecting plants for more than 9 weeks and 6 week in the greenhouse and field. PGPF-induced plants limited pathogen spore germination and decreased the number of infection hyphae on the leaf, and increased lignification at places of attempted pathogen infection, thus reducing the pathogen spread. PGPF elicited increased activities of chitinascs, glucanases, peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase to C. orbiculare infection in cucumber plants. The role of PGPF in elevating cucumber defense response to pathogen infection suggests potential application of PGPF as biological control agents.

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Diagnosis of Graft Infection Using FDG PET-CT

  • Shim, Hun-Bo;Sung, Ki-Ick;Kim, Wook-Sung;Lee, Young-Tak;Park, Pyo-Won;Jeong, Dong-Seop
    • Journal of Chest Surgery
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    • v.45 no.3
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    • pp.189-191
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    • 2012
  • Graft infections after aortic replacement are a rare, but severe complication. Because surgical removal of the infection source is essential, an accurate diagnosis is required to prevent unnecessary treatment. Both of the patients described herein were diagnosed with graft infections using dual-modality positron emission tomography-computed tomography; one patient was a false-positive, and the other was confirmed with an infection.

Effect of Dual Inoculation with Glomus mosseae and Rhizobium meliloti on Growth and Nitrogenase Activity of Medicago sativa L. (Glomus mosseae와 Rhizobium meliloti의 동시접종이 알팔파의 생장 및 질소고정활성에 미치는 영향)

  • 유익동;서현창;김원곤;박동진;조갑연;김창진
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.95-100
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    • 1993
  • Effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R455 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as as result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection.

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Successful Removal of 15-year-old Pacemaker Leads by Weight and pulley method

  • Kim, Hyun-Woo;Shin, Ho-Cheol;Jin, Han-Young;Seo, Jeong-Sook;Jang, Jae-Sik;Yang, Tae-Hyun;Kim, Dae-Kyeong;Kim, Dong-Soo
    • Kosin Medical Journal
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    • v.33 no.3
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    • pp.396-401
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    • 2018
  • Extraction of old pacemaker leads remains a complex procedure owing to fibrotic encapsulation and lead adhesions. We report a case of extraction of 15-year-old pacemaker leads by weight and pulley method. A 81-year-old man presented with exposed pacemaker leads out of body with purulent discharge from a pacemaker insertion site. He inserted DDD (dual chamber pacing, dual chamber sensing dual function) pacemaker implantation 15 years ago for SSS. Previously pacemaker battery was removed 3 years ago due to recurrent infection of pacemaker scar site. We extracted the pacemaker leads by weight and pulley method successfully without any complications.

Transareolar-Perinipple Dual Pockets Breast Augmentation (횡유륜 유두주위절개를 통한 이중포켓 유방확대술)

  • Lee, Paik Kwon;Kim, Jee Hoon;Seo, Byung Chul;Oh, Deuk Young;Rhie, Jong Won;Ahn, Snag Tae
    • Archives of Plastic Surgery
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    • v.34 no.1
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    • pp.93-98
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    • 2007
  • Purpose: Many options are available for the incision and pocket selection in breast augmentation. Each method has its advantages and disadvantages. To leave an invisible operation scar and to achieve easier pocket dissection by the central location of the incision on the breast, we made a transareolar-perinipple incision. To overcome the disadvantages of the transareolar incision, originally advocated by Pitanguy in 1973, we modified the direction of incision line and dissection plane. Methods: To avoid the injury of 4th intercostal nerve responsible for nipple sensation, we made perinipple incision on the medial side of the nipple instead of trans-nipple incision and made the transareolar incision as 11-5 o'clock on the left side and 1-7 o'clock on the right side instead of 3-9 o'clock on both sides. To avoid the possible infection and breast feeding problem caused by the injury to the lactiferous duct, and the possible implant hernia caused by the incisions lying on a same plane of pocket dissection, we made a subcutaneous dissection just above the breast tissue medially down to the bottom of breast tissue and made a subglandular or subfascial pocket, which may avoid the injury of lactiferous duct and create different planes for skin incision and pocket dissection. Other advantages of the transareolar-perinipple incision include easier pocket dissection, less chance of hematoma, and as a result less postoperative pain because of the central location of the approach which allow finger dissection and meticulous bleeding control with direct vision, without any specialized instrument such as an endoscope or long mammary dissectors. As for pocket selection, we made dual pockets. We prefer subglandular or subfascial pocket. Also, we made a subpectoral pocket in the upper 1/4 of the pocket to add more volume on the upper part of the augmented breast, which can make aesthetically more desirable breasts in thin Asian women with small breasts. Possible disadvantages of our method are subclinical infection and scar widening, which could be overcome by meticulous operation techniques, antibiotic therapy, and intradermal tattooing. Results: From September, 2003 to August, 2005, 12 patients underwent breast augmentation using round smooth surface saline implants by our method. During the mean follow-up period of 13 months, there were no complications such as infection, hematoma, capsular contracture, and sensory change of nipple, and results were satisfactory. Conclusion: We suggest breast augmentation via transareolar-perinipple incision and dual pockets(subpectoral-subglandular or subfascial) as a valuable method in thin oriental women with small breasts.