• Title/Summary/Keyword: Dry leaves

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Effects of Short Microwave Irradiation Time at the Seedlings Stage on the Growth and Secondary Metabolite Contents of Lettuce (Lactuca sativa L.) (유묘단계에서 단시간 마이크로웨이브 처리가 상추의 생육 및 이차대사산물 함량에 미치는 영향)

  • Yong Jae Lee;Su Yong Park;Ju Hyung Shin;Seung Yong Hahm;Gwang Ya Lee;Jong Seok Park
    • Journal of Bio-Environment Control
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    • v.32 no.3
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    • pp.217-225
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    • 2023
  • This experiment was conducted to investigate the effects of microwave irradiation on the growth and secondary metabolite contents of lettuce seedlings. Seedlings at three weeks after sowing were treated by a microwave oven for 0, 4, 8, and 12 seconds with 200 W. After cultivation in a close plant production system for 4 weeks, plant growth measurements and secondary metabolite analysis were performed. The results showed that the fresh and dry weights of the shoot and root, leaf area, leaf length, and the number of leaves were decreased as increasing the microwave treatment times. Chlorophyll a and b, total carotenoids were increased and total phenolics were decreased at the 12-second treatment compared to the 4-second treatment. Total flavonoid contents were decreased at the 8-second treatment compared to the control. These results suggest that the changes in the levels of secondary metabolites were caused by oxidative stress. Although there was no significant difference in secondary metabolite contents excluding total flavonoid contents on the microwave treatments compared to the control, the significant difference suggests that the microwave treatment of 200 W and 2.45 GHz may alter secondary metabolite contents of lettuce after 4 weeks.

Reduction of Stress Caused by Drought and Salt in Rice (Oryza sativa L.) Crops through Applications of Selected Plant Extracts and the Physiological Response Mechanisms of Rice

  • Hyun Hwa Park;Young Seon Lee;Yong In Kuk
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.57-57
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    • 2022
  • In many areas of the world, salt damage and drought have had a negative impact on human survival due to a decrease in agricultural productivity. For instance, about 50% of agricultural land will be affected by salt damage by 2050. Biostimulants such as plant extracts can not only increase the nutrient utilization efficiency of plants, but also promote plant growth and increase resistance to abiotic or biotic stress. Therefore, the objective of this study was to determine how selected plant extracts might reduce levels of stress caused by drought and salt and to better understand the physiological response mechanisms of rice plants. In this study, we used Soybean leaves, Soybean stems and Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts were used. These extracts had been used in previous studies and were found to be effective. The materials were dried in a dry oven at 50℃ for 5 days and ground using a blender. Each 50 g of materials was put in 1 L of distilled water, stirred for 24 hours, filtered using 4 layers of mirocloth, and then concentrated using a concentrator. Rice (cv. Hopumbyeo) seeds were immersed and germinated, and then sown in seedbeds filled with commercial soil. In drought experiments, three rice seedlings at 1 week after seeding was transplanted into 100 ml cups filled with commercial soils and grown until the 4-leaf stage. For this experiment, the soil weight in a cup was equalized, and water was allowed to become 100% saturated and then drained for 24 hours. Thereafter, plant extracts at 3% concentrations were applied to the soils. For NaCl treatments, rice plants at 17 days after seeding were treated with either 100 mM NaCl or plant extracts at 1%+ 100 mM NaCl combinations in the growth chamber. Leaf injury, relative water content, photosynthetic efficiency, and chlorophyll contents were measured at 3, 5, and 6 days after treatments. Shoot fresh weight of rice under drought conditions increased 28-37% in response to treatments of Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Shoot fresh weight of rice subjected to 100 mM NaCl treatments also increased by 6-24% in response to Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Compared to the control, rice plants treated with these six extracts and subjected to drought conditions had significantly higher relative water content, Fv/Fm, total chlorophyll and total carotenoids than control plants. With the exception of relative water contents, rice plants treated with the six extracts and subjected to salt stress (100 mM NaCl treatments) had significantly higher Fv/Fm, total chlorophyll and total carotenoids than control plants. However, the type of extract used did not produce significant difference in these parameters. Thus, all the plant extracts used in this study could mitigate drought and NaCl stresses and could also contribute substantially to sustainable crop production.

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Effect of Air Temperature on Growth and Phytochemical Content of Beet and Ssamchoo (온도처리가 비트와 쌈추의 생육과 생리활성 물질 함량에 미치는 영향)

  • Lee, Sang Gyu;Choi, Chang Sun;Lee, Hee Ju;Jang, Yoon Ah;Lee, Jun Gu
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.303-308
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    • 2015
  • The consumption of leaf vegetables has been steadily increasing in Korea. Leaf vegetables are used for "Ssam (vegetable wrap-up), eaf vegetables has been steadily increasing in Korea. Leaf vegetables are used for asoned condiments inside several layers of young vegetable leaves. This study investigated the effect of air temperature on the growth and phytochemical contents of beet (Beta vulgaris L.) and Ssamchoo (Brassica lee L. ssp. namai) grown in a closed-type plant factory system where fluorescent lamps were used as an artificial light source. Seeds of beet and Ssamchoo were sown in a peat-lite germination mix. The roots of 20-day-old seedlings were washed, and the seedlings were planted on a styrofoam board and grown in hydroponic beds for 25 days under fluorescent light. Plants were exposed to one of three different air temperature regimes (20, 25 and $30^{\circ}C$ during the day combined with $18^{\circ}C$ during the night), which were monitored with a sensor at 30 cm above the plant canopy. Increased plant height and leaf area were observed in beet at $25^{\circ}C$ and $30^{\circ}C$ compared to $20^{\circ}C$. For Ssamchoo, the greatest plant height, leaf area, fresh weight and dry weight were obtained at $20^{\circ}C$. Ascorbic acid content of beet and Ssamchoo leaves were highest at $30^{\circ}C$. In beet, total polyphenol and flavonoid contents were higher at $20^{\circ}C$ (42.4, $197.0mg{\cdot}g^{-1}DW$) and $25^{\circ}C$ (46.9, $217.0mg{\cdot}g^{-1}DW$) than $30^{\circ}C$ (22.4, $88.0mg{\cdot}g^{-1}DW$). In Ssamchoo, total polyphenol and flavonoid contents were also higher at $20^{\circ}C$ (79.2, $268.2mg{\cdot}g^{-1}DW$) and $25^{\circ}C$ (66.3, $258.3mg{\cdot}g^{-1}DW$), respectively, than $30^{\circ}C$ (53.7, $134.7mg{\cdot}g^{-1}DW$). Hence, the optimum temperature appears to be $20^{\circ}C$ for growing both beet and Ssamchoo in a closed-type plant factory system with fluorescent light.

Growth and Phytochemicals of Lettuce as Affected by Light Quality of Discharge Lamps (방전램프의 광질에 따른 상추의 생장 및 파이토케미컬 분석)

  • Lee, Jae Su;Nam, Sang Woon;Kim, Yong Hyeon
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.400-407
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    • 2013
  • This study was performed to analyze the effect of light quality of discharge lamp on growth and phytochemicals contents of lettuce (Lactuca sativa L. cv. Jeokchima) grown under metal halide (MH) lamp, high-pressure sodium (HPS) lamp, and xenon (XE) lamp in a plant factory. Cool-white fluorescent (FL) lamp was used as the control. Photoperiod, air temperature, relative humidity, $CO_2$ concentration, and photosynthetic photon flux (PPF) in a plant factory were 16/8 h (day/night), $22/18^{\circ}C$, 70%, 400 ${\mu}mol{\cdot}mol^{-1}$, and 200 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. MH lamp had the greatest fraction of blue light (400-500 nm) of 23.0%. However, HPS lamp had the lowest fraction of 4.7% for blue light and the greatest fraction of 38.0% for red light (600-700 nm). At 11 and 21 days after transplanting, leaf length, leaf width, leaf area, shoot fresh weight, and shoot dry weight of lettuce as affected by the light quality of the discharge lamp were significantly different. The leaf area of lettuce grown under HPS, MH, and XE lamp increased by 45.7%, 16.3%, and 9.5%, respectively, as compared to the control. These results were similar for shoot fresh weight. Growth characteristics of lettuce grown under HPS lamp increased since HPS lamp had more fraction of red light. However, growth of lettuce grown under MH and XE lamp decreased since they had more fraction of blue light. As compared to the control, the ascorbic acid in lettuce leaves grown under discharge lamp decreased. The greatest anthocyanins accumulation of 0.70 mg/100 g was found at MH treatment. Anthocyanins content in lettuce leaves grown under XL and HPS lamp were 79.3% and 8.6%, respectively, compared with the control. Growth and phytochemicals contents of lettuce were highly affected by the different spectral distribution of the discharge lamp. These results indicate that the combination of discharge lamp or LED lamp for enhancing the light quality of discharge lamps is required to increase the growth and phytochemicals accumulation of lettuce in controlled environment such as plant factory.

Antifungal and Antioxidant Activities of Extracts from Leaves and Flowers of Camellia japonica L. (동백나무 잎과 꽃 추출물의 항미생물 활성 및 항산화 효과)

  • Lee, Sook-Young;Hwang, Eun-Ju;Kim, Gi-Hae;Choi, Young-Bok;Lim, Chae-Young;Kim, Sun-Min
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.93-100
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    • 2005
  • This research was performed to investigate the possibilities of industrial usage of camellia (Camellia japonica L.) by examining the antioxidant and antimicrobial effects of methanol extract with different sections. Content of total phenolics, DPPH radical scavenging activities and antibacterial activity of young leaf, mature leaf, flower bud, flower, bark, and seed of camellia were compared in vitro experimental models. Total phenolics was contained the higher in young leaf (74.62 mg), flower bud (65.02 mg) and flower (62.42 mg) but less than 20.95 mg per 100 g of dry weight in other parts of Camellia japonica L. And effects of antioxidant measured by DPPH radical scavenger activity ($RC_{50}$, reduce concentration 50%), was shown higher $7.16{\sim}18.14\;{\mu}g/m{\ell}$ in methanol extract of young leaf, flower bud and flower than $61.23\;{\mu}g/m{\ell}$ of BHT as a chemical oxidant. Also, the antimicrobial activity of Camellia japonica L. extracts determined using a paper disc method against food-borne pathogen and food spoilage bacteria, the young leaves extracts showed the most active antimicrobial activity against 7 kinds of harmful microorganisms. Flower bud extracts showed the highest antibacterial activity against P. aeruginosa and Enterobacter spp. C1036. In addition, the minimum inhibitory concentration (MIC) of young leaf extract against B. subtillis,S. fradiae,S. aureus,E. coli,P. aeruginosa, Enterobacter spp. C1036, and S. typhimurium were revealed 1 to 15 ${\mu}g/m{\ell}$. As a result, antimicrobial activity of camellia extracts was shown higher gram positive bacteria than gram negative bacteria.

Seed Germination, Seedling Growth and Biomass Production of Eucommia ulmoides (두충(杜冲)나무의 종자발아(種子發芽), 묘목생장(苗木生長) 및 물질생산(物質生產))

  • Goo, Gwan Hyo;Youn, Ki Sik;Lee, Kang Young
    • Journal of Korean Society of Forest Science
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    • v.80 no.2
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    • pp.202-209
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    • 1991
  • This study was carried out to examine seed germination, seedling growth, and biomass production of Eucommia ulmoides. The results were summarized as follows ; 1. Cold moist stratification at a refrigerator showed 60.3% of seed germination, which is the most effective among pretreatment methods. 2. In case of 1-0 seedlings, sixty seedlings per $1m^2$ was the most appropriate density, and the growth quality of seedlings differed significantly among densities. 3. Height growth showed continuous growth pattern and the maximum growth was shown during the period 21 June to 20 July, which corresponds 32.7% of total annual growth. 4. As tree age and DBH increased, the production of bark and leaf increased, however, its increment rate remarkably dropped down after ten years. 5. When tree age was ten years, the rate of bark volume showed the maximum value, 12.42%. 6. Dry weight of barks and leaves and bark volume were positively related to the growth of DBH and height. 7. As tree age increased, leaf area decreased, but the number of leaves increased.

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Influence of Fertilization Treatment using Organic Amendment based on Soil Testing on Plant Growth and Nutrient Use Efficiency in Potato (토양검정에 의한 유기자원 시비처방이 감자의 생육 및 양분이용효율에 미치는 영향)

  • Lim, Jin-Soo;Lee, Bang-Hyun;Kang, Seung-Hee;Lee, Tae-Guen
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.436-446
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    • 2020
  • In this study, we verified the effects of organic amendment application based on soil testing in potato cultivation. The application rate of organic amendments was determined based on the inorganicization rate of nitrogen, phosphoric acid, and potassium in the organic amendments. There was no significant difference in the length, stalk number, stalk diameter, and leaf color of potato plants under organic amendment application compared with those under chemical fertilization. The quantity of potato tubers and yield of marketable tubers under organic amendment application were higher than those under chemical fertilization. The top fresh weight and top-to-root ratio were the highest under organic amendment application, followed by those under chemical fertilization and the control. The inorganic nitrogen content in the leaves and stems of potato plants was the highest under chemical fertilization. There was no difference in the phosphoric acid content between the potatoes under chemical fertilization and those under organic amendment application. The potassium content in potatoes was higher under chemical fertilization than that under organic amendment application. While the change trend of inorganic nitrogen content in the roots was similar to that in the leaves and stems, the potatoes under organic amendment application, with the highest yield, showed the highest dry weight. The tuber weight showed a positive correlation with plant length, stalk number, and stalk diameter. The higher the weight of the tuber, the higher the weight of the marketable tuber, and the higher the top fresh weight, the higher the yield. Therefore, to increase yield, it is necessary to supply nutrients to improve the top fresh weight to the optimal level. There was no difference in the utilization efficiency of nitrogen and potassium between the potatoes under chemical fertilization and those under organic amendment application. The utilization efficiency of phosphoric acid was increased by 0.1% in potatoes under organic amendment application compared with that under other treatments. Regarding soil chemistry after harvest, the soils under organic amendment application showed a higher electrical conductivity and higher nitrogen and phosphoric acid content than those under other treatments. Therefore, the organic amendment method used in this study can be an alternative to chemical fertilization. It is also advantageous for the cultivation of subsequent crops in potato fields.

Enhancement of Stem Firmness in Standard Chrysanthemum 'Baekma' by Foliar Spray of Liquid Calcium Compounds (액상 칼슘 화합물 엽면살포에 의한 스탠다드 국화 '백마'의 줄기 경도 강화)

  • Lee, Chang-Hee;Nam, Mi-Kyong
    • Horticultural Science & Technology
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    • v.29 no.4
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    • pp.298-305
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    • 2011
  • This study was conducted to enhance the stem firmness of standard chrysanthemum 'Baekma' bred in Korea for commercial quality improvement and inhibition of stem breaking during transportation through foliar spray with calcium agents. Calcium agent screening 'Baekma' was examined using $CaCl_2{\cdot}2H_2O$, $Ca(NO_3)_2{\cdot}4H_2O$, and OS-Ca (natural liquid calcium compounds extracted from oyster shell) depending on each concentration (0, 0.001, 0.01, 0.1, and 1.0%, respectively). All calcium agents sprayed with 1.0% caused chemical injury such as stem bending or leaf burn. OS-Ca also showed more sensitive response to chemical injury than the other calcium agents because OS-Ca was absorbed very well by 'Baekma' leaves. Maximum stem firmness measured during the final harvest was greater in OS-Ca than in the other calcium agents. Especially, maximum stem firmness was greatest in 0.01% OS-Ca. However, elastic strength and maximum bending stress were greater in 0.001% OS-Ca than in the others. Thus, OS-Ca ranged from 0.005 to 0.05%, which did not show any chemical injury, was finally selected as the first candidate for hardening the stem of 'Baekma'. The next experiment using OS-Ca was conducted with the concentrations of 0, 0.005, 0.01, and 0.05%, respectively. From the results, 0.05% OS-Ca showed better plant growth and parameters such as plant height, stem diameter (upper and middle part), the number of leaves, and dry weights of each part than the other concentrations of OS-Ca and control. As for stem firmness depending on OS-Ca concentration, the Ca content within stem, maximum firmness, elastic strength, and maximum bending stress of stem in 'Baekma' sprayed with 0.05% OS-Ca showed the highest values among all the treatments and it turned out to be very high level of significance between control and OS-Ca treatments. However, the area and percentage of the inside cavity within horizontal stem section in 'Baekma' did not show any significance between any treatments including control. Thus, stem firmness of 'Baekma' did not show any correlation with the inside cavity area of stem. In conclusion, we recommend foliar sprays with 0.05% OS-Ca at vegetative growth stage to enhance stem firmness of 'Baekma' during transportation.

The Evaluation of Feed Value and Growth Characteristics of Sasa quelpaertenisis Nakai by Horse Grazing in the Woodland of Jeju (말 방목에 의한 제주조릿대의 생육특성 및 사료 가치 평가)

  • Lee, Chong-Eon;Kim, Hyun-Cheol;Whang, Kyung-Joon;Park, Nam-Geon;Kim, Nam-Young;Oh, Woon-Young
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.2
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    • pp.151-158
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    • 2010
  • This study was conducted to evaluate the feed value and growth characteristics of Sasa quelpaertensis Nakai by horse grazing in the woodland of Jeju. Crude protein (CP), NDF, ADF and minerals contents at different growth stages of Sasa quelpaertensis were analyzed. The apparent digestibilities of DM, CP, NDF and ADF were determined by total collection method using 5 Jeju horses. The yield and growth characteristics of S. quelpaertensis Nakai by horse grazing were investigated. CP contents in the currentand the previous-year-sprouted leaves of S. quelpaertenisis were $16.6{\pm}1.0%$ and $12.2{\pm}1.1%$, respectively. The digestibility of DM, CP, NDF and ADF in the current-year-sprouted leaves of S. quelpaertenisis were $47.2{\pm}1.2$, $67.8{\pm}1.0$, $47.0{\pm}1.3$ and $47.1{\pm}1.1%$, respectively. The current-year-sprouted S. quelpaertenisis tended to have high nutritional values and digestibilities compared to those of the previous-year-sprouted. The dry matter yield, plant length, leaf width and density of S. quelpaertensis Nakai were clearly decreased by horse grazing. These results show that considering the feed value and growth characteristics, the natural S. quelpaertenisis Nakai can be used as a feed source for horses.

Influence of Gibberellin and Urea treatment on the Production of mulberry leaf and characteristic of silkworm (지베렐린과 요소의 처리가 상수의 수량 및 잠아의 실용형질에 미치는 영향)

  • 유근섭;오준식
    • Journal of Sericultural and Entomological Science
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    • v.9
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    • pp.27-34
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    • 1969
  • This study was conducted investigate the effect of Gibberellin and Urea on the growth of mulberry tree with the compound treatment, and the characteristic of silkworm when the mulberry leaves simply treated with GB and the compound treatment with GB and Urea were supplied. The results are as follows. 1. Mulberry tree A. The leaf weight per mulberry tree with the compound treatment of GB+ Urea was heavier in spring silkworm and the leaf yield was increased by 7% compared with the non-treatment plot. B. The growth of wattle, leaf length and leaf width was more accelerated in the compound treatment plot of GB+Urea, the single treatment plot of GB and Urea than th!: non-treatment plot in autumn silkworm, and there was observed an incredse yielding effect of 16% in GB+Urea plot, in 16% GB plot and 18% in Urea plot respectively as compared with th: non-treatment plot, hut yield per l000m unit of wattle length was the least in GB plot. C. In the composition of mulberry leaves of treatment, water and crude protein was increased in the compound treatment plot of GB + Urea and the single treatment plot of GB and Urea than the non-treatment plot, but dry matter and carbohydrate were decredsed. 2. Characteristics of Larva in spring silkworm A. The mortality ratio of 4 and 5 instar plot was equally high in the compound treatment plot of GB+ Urea and the pupation ratio of 4 instar plot was lower than other treatment plot. B. Although there was no significant difference observed in the cocoon weight of 10.000 1st-day worms in both 4 and 5 instar plots between each treatment plot, the single treatment plot of GB showed somewhat great than other treatment plots. C. There was no significant difference observed in the cocoon layer ratio between each treatment plot, but 4 instar plots was slightly higher than 5 instar plots. 3. Characteristics of Larva in Autumn silkworm A. The mortality ratio of the compound treatment plot of GB+ Urea and GB was higher than that of the nontreatment and single treatment plot of Urea. B. The pupation ratio of the single treatment plot of Urea was higher than that of the compound treatment plot of GB + Urea, and there was no significant difference observed between other treatment plot. C. The cocoon weight of 10.000 1st-day th instar worms was heavier in the single treatment plot of Urea than nontreatment plot, GB+Urea plot and GB plot. D. The cocoon layer ratio was no significant difference observed between each-treatment.

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