• KSBB Journal
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• 제4권3호
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• pp.271-275
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• 1989

본 논문에서는 Thalictrum rugosum 식물세포의 회분식 배양에 있어서 동역학적 특성들을 연구하였다. 본 실험에서 alkaloid의 생성은 일반적인 2차대사 산물의 생성 특성과 달리 세포성장에 비례함이 확인되었다. 세포 성장기의 비증식속도는 $0.20~0.25\;day\;^{-1}이었으며 FW / DCW 비가 배양상태를 나타내는 중요한 인자임을 알수 있었다. Cell yield는 0.36g cells/g sugar이었으며 berberine은 139mg/L까지 생성되었고 그중 120mg/L는 세포내에 존재하였다. 이를 specific content로 계산하면 dry cell weight의 1.10%가 된다. 세포 성장기에서 비증식속도와 당농도간의 관계는 Monod kinetics로 잘 기술될 수 있었다.

• Journal of Microbiology
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• 제33권4호
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• pp.278-282
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• 1995

S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.140-146
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• 1999

When methanol was the sole carbon source, Methylobacterium organophilum NCIB 11278, a facultative methylotroph, accumulated Poly-$\beta$-hydroxybutyrate (PHB) as 59% (w/w) of dry cell weight under potassium limitation, 37% under sulfate limitation, and 33% under nitrogen limitation. Based on a stoichiometric analysis of PHB synthesis from methanol, it was suspected that PHB synthesis is accompanied by the overproduction of energy, either 6-10 ATP and 1 $FADH_2$ or 6 ATP and 3 NADPH to balance the NADH requirement, per PHB monomer. This was confirmed by observation of increased intracellular ATP levels during PHB accumulation. The intracellular ATP with limited potassium, sulfate, and ammonium increased to 0.185, 0.452, and 0.390 $\mu$moles ATP/g Xr (residual cell mass) during PHB accumulation, respectively. The intracellular ATP level under potassium limitation was similar to that when there was no nutrient limitation and no PHB accumulation, 0.152- 0.186 $\mu$moles ATP/g Xr. We propose that the maximum PHB accumulation observed when potassium was limited is a result of the energy balance during PHB accumulation. Microorganisms have high energy requirements under potassium limitation. Enhanced PHB accumulation, in ammonium and sulfate limited conditions with the addition of 2,4-dinitrophenol, which dissipates surplus energy, proves this assumption. With the addition of 1 mM of 2,4-dinitrophenol, the PHB content increased from 32.4% to 58.5% of dry cell weight when nitrogen limited and from 15.1 % to 31.0% of dry cell weight when sulfate limited.

• 한국해양바이오학회지
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• 제2권3호
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• pp.198-200
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• 2007

Cultivation of a $Q_{10}$-producing photosynthetic bacterium, Rhodobacter sphaeroids, was carried out in a 1-L flask to characterize its cellular growth reaction. The result of experiment showed that dissolve oxygen in the broth was depleted within 7 h. ORP decreased with decrease of DO, and recovered somewhat with increase of pH. The growth of R. spahaeroids reached at late-log phase in 140 h of reaction. The final pH and dry-cell weight were 7.62 and 2.2 mg/mL, respectively. The $Q_{10}$ content in the final broth was 2.35 mg/g dry cell weight, which was higher than that obtained in tube culture.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.347-350
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• 2001

The dextran prodution by Leuconostoc mensenteroids NRRL-B512F was studied in a synthetic medium from sucrose as a sole carbon source. Especially, effect of nitrogen source was treated and compared in this study. In oder to maximize the cell growth and dextran produtivity through fermentation two nitrogen sources, yeast extract and tryptone, were used with various concentrations. At the end of fermentation, when the concentration of yeast extract was 9g/L we can obtain the maximum dry cell weight(14.1g/L), dextran dry weight(25.4g/L) and productivity(1.4g/L ${\cdot}$ hr).

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.162-163
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• 2003

수소를 생산하는 암반응에서는 pH와 dry cell weight, 세포내의 starch 함유량이 시간에 따라 줄어들었다. 유기물은 시간에 따라 증가하였지만, 수소는 3일째에 생산이 최대치에 도달하였다.

• 대한의생명과학회지
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• 제14권2호
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• pp.91-96
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• 2008

Light Emitting Diode (LED) of 880 nm was used as a function of luminosity in culture of the photosynthetic bacteria including Rhodobacter sp.. An array of 880 run LED was driven with an energy density of $6.0mW/cm^2$. In processing time, we were able to show that the cell growth were gained of significant changes in the pigment and in the dry weight. And we also showed that photosynthetic bacteria had the resonable relativity of optical density to dry weight. LED-880nm is of great significance for the potential use of photo-bioreactor construction.

• 한국식품영양학회지
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• 제11권6호
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• pp.617-621
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• 1998

The production of red pigment from glucose by fed-batch culture of Monascus anka was investigated. In batch culture using fermentor, 200 rpm of agitation speed, 1vvm of aeration volume, and 10% (v/v) of inoculum size were optimal, respectively. The red pigment production was increased by removal of wall-attached mycelium. In an intermittent feeding fed-batch culture, dry cell weight increased to 30 g/l, adn the red pigment content reached 350 of absorbance at 495nm. In a continuous feeding fed-batch culture, dry cell weight increased to 22g/l, and the red pigment content reached 190 of absorbance at 495nm.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.412-415
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• 1998

The accumulation process of $Pb^{2＋}$ in an industrial strain of Saccharomyces cerevisiae proved to be temperature-dependent, and was quite similar to chemical adsorption at the initial stage of $Pb^{2＋}$accumulation. The initial $Pb^{2＋}$ accumulation rate increased from 11.4 to 46.2 mg $Pb^{2＋}$/g cell dry weight/day, in response to the increased temperature from $20^{\circ}C\;to\;50^{\circ}C$ while the maximal $Pb^{2＋}$ accumulation amount (175.8 mg $Pb^{2＋}$/g cell dry weight) was achieved at $30^{\circ}C$. The maximal $Pb^{2＋}$/ accumulation amount with temperature was independent of ion exchange with $K^＋\;and\;Mg^{2+}$.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.291-294
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• 2000

The production of polyhydroxybutrate(PHB) by Alcaligenes eutrophus NCIB 11599 was studied in a synthetic medium from whey as a sole carbon source. Especially pH-effect was treated and compared in this study. At the end of fermentation (A) unadjusted to pH, the dry cell weight, PHB concentration, and PHB conversion rate were 10.3g/L, 3.1g/L, and 30%, respectively. At the end of fermentation (B) adjusted to $pH(7.0{\sim}7.5)$, the dry cell weight, PHB concentration, and PHB conversion rate were 12.5g/L, 4.8g/L, and 41%, respectively. PHB conversion rate was about 10% higher on the fermentation (B) than on the fermentation (A).