• Korean Journal of Environment and Ecology
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• v.28 no.3
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• pp.357-364
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• 2014

This study aimed to determine effects of the use of water-retention additive, hydrophilic polymer, for extensive green roofs on growth of Juniperus chinensis var. sargentii and Euonymus fortunei 'Emerald and Gold' for woody plants, and Carex kobomugi and Carex pumila for herbaceous plants. Five different contents of hydrophilic polymer including 0% (Control), 1.0%, 2.5%, 5.0%, and 10% (polymer: medium (w/w), dry weight basis) were added to each of the container filed with a 100 kg of growth medium. Ten of plants were transplanted in each of square container ($1m(L){\times}1m(W){\times}0.3m$ (H)) built on the roof platforms in randomized complete block design in the $20^{th}$ of May, 2013. In results, excessively high volumetric soil water content, about 97-98%, was found in the substrate under elevated hydrophilic polymer concentration of at least 2.5%, during the entire growing period. The moisture content of the substrate containing 1.0% of hydrophilic polymer was higher about 20% in the range between 70% and 80%, compared tho that of Control substrate in the range between 50% and 60%, for 27 days after transplanting prior to abundant rainfall, indicating that the application of hydrophilic polymer to the extensive green roof substrate is effective to eliminate drought condition by retaining water in the substrate. Euonymus fortunei 'Emerald and Gold' and Carex kobomugi resulting in higher plant growth with 2.5% than those of the other treatment plants. Juniperus chinensis var. sargentii was observed the highest growth under 1.0% hydrophilic polymer treatement, and Carex pumila was founded the best growth with Control respectively. Plants that grown in both the 1.0% and 2.5% hydrophilic polymer survived all, while the plants that grown in the 5.0% and 10% hydrophilic polymer died after 3 months. These results suggest that advantage of the addition of hydrophilic polymer may be greater in drought-tolerant plants, but the mixture proportion of hydrophilic polymer should be determined according to the different features of the plant species being grown.

• Korean Journal of Organic Agriculture
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• v.26 no.2
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• pp.217-232
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• 2018

Drought is a major obstacle to high agricultural productivity, worldwide. In drought, it is usually presented by the simultaneous action of high temperature and drying. Also there are negative effects of plant growth under drying conditions. In this study, the effect of Bacillus velezensis YP2 on plant growth-promotion and soil drying stress tolerance of kale plants, Brassica oleracea var. alboglabra Bailey, were investigated under two different conditions; greenhouse and field environments. Root colonization ability of B. velezensis YP2 was also analysed by using plating culture method. As a result of the greenhouse test, the YP2 strain significantly promoted the growth of kale seedlings in increasement of 26.7% of plant height and 142.2% of shoot fresh weight compared to control. B. velezensis YP2 have the mitigation effect of drying injury of kale by decreasing of 39.4% compared to control. In the field test, B. velezensis YP2 strain was also found to be effective for plant growth-promotion and mitigation of drying stress injury on kale plants. Especially, relative water contents (RWC; %) were higher in B. velezensis YP2 treated kales than in control at 7, 10, 14 day after non-watering. The root colonization ability of YP2 strain was continued at least for 21 days after soil drenching treatment of B. velezensis YP2. Our result suggested that enhancement of plant growth and drying injury reduction of kale plants were involved in kale root colonization by B. velezensis YP2, which might be contributed to increasing water availability of plants. Consequentially, the use of B. velezensis YP2 might be a beneficial influence for improving productivity of kale plants under drying stress conditions.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.8-9
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• 2017

Soybean yield has been low and unstable in Japan and other areas in East Asia, despite long history of cultivation. This is contrasting with consistent increase of yield in North and South America. This presentation tries to describe perspective of breaking stagnation of soybean yield in East Asia, considering the factors of the different yields between regions. Large amount of rainfall with occasional dry-spell in the summer is a nature of monsoon climate and as frequently stated excess water is the factor of low and unstable soybean yield. For example, there exists a great deal of field-to-field variation in yield of 'Tanbaguro' soybean, which is reputed for high market value and thus cultivated intensively and this results in low average yield. According to our field survey, a major portion of yield variation occurs in early growth period. Soybean production on drained paddy fields is also vulnerable to drought stress after flowering. An analysis at the above study site demonstrated a substantial field-to-field variation of canopy transpiration activity in the mid-summer, but the variation of pod-set was not as large as that of early growth. As frequently mentioned by the contest winners of good practice farming, avoidance of excess water problem in the early growth period is of greatest importance. A series of technological development took place in Japan in crop management for stable crop establishment and growth, that includes seed-bed preparation with ridge and/or chisel ploughing, adjustment of seed moisture content, seed treatment with mancozeb+metalaxyl and the water table control system, FOEAS. A unique success is seen in the tidal swamp area in South Sumatra with the Saturated Soil Culture (SSC), which is for managing acidity problem of pyrite soils. In 2016, an average yield of $2.4tha^{-1}$ was recorded for a 450 ha area with SSC (Ghulamahdi 2017, personal communication). This is a sort of raised bed culture and thus the moisture condition is kept markedly stable during growth period. For genetic control, too, many attempts are on-going for better emergence and plant growth after emergence under excess water. There seems to exist two aspects of excess water resistance, one related to phytophthora resistance and the other with better growth under excess water. The improvement for the latter is particularly challenging and genomic approach is expected to be effectively utilized. The crop model simulation would estimate/evaluate the impact of environmental and genetic factors. But comprehensive crop models for soybean are mainly for cultivations on upland fields and crop response to excess water is not fully accounted for. A soybean model for production on drained paddy fields under monsoon climate is demanded to coordinate technological development under changing climate. We recently recognized that the yield potential of recent US cultivars is greater than that of Japanese cultivars and this also may be responsible for different yield trends. Cultivar comparisons proved that higher yields are associated with greater biomass production specifically during early seed filling, in which high and well sustained activity of leaf gas exchange is related. In fact, the leaf stomatal conductance is considered to have been improved during last a couple of decades in the USA through selections for high yield in several crop species. It is suspected that priority to product quality of soybean as food crop, especially large seed size in Japan, did not allow efficient improvement of productivity. We also recently found a substantial variation of yielding performance under an environment of Indonesia among divergent cultivars from tropical and temperate regions through in a part biomass productivity. Gas exchange activity again seems to be involved. Unlike in North America where transpiration adjustment is considered necessary to avoid terminal drought, under the monsoon climate with wet summer plants with higher activity of gas exchange than current level might be advantageous. In order to explore higher or better-adjusted canopy function, the methodological development is demanded for canopy-level evaluation of transpiration activity. The stagnation of soybean yield would be broken through controlling variable water environment and breeding efforts to improve the quality-oriented cultivars for stable and high yield.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.51-56
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• 2015

The objective of this study was to evaluate the effect of silicate fertilizer on growth, physiology and abiotic stress tolerance of Chinese cabbage seedlings. Five silicate concentrations (8, 16, 32, 64, and 128mM) and control (non-treatment) were applied to Chinese cabbage seedlings twice a week. Three weeks after application of silicate treatment, seedlings were used for treating abiotic stresses and were assessed for growth and physiological characteristics. Growth parameters significantly increased in 8, 16, and 32mM treatments except 64 and 128mM. Total root surface area, total root length, and number of root tips increased in 8, 16 and 32mM treatments, but they decreased in treated seedlings with 64 and 128mM of silicate. The highest growth parameters and root morphology were observed in 8mM treatment. As for the effect on the seedling physiology, transpiration rates decreased while stomatal diffusive resistance increased to increasing silicate concentration. The application of silicate reduced the electrical conductivity, heating and chilling injury index at high and low temperatures. Silicate enhanced drought tolerance of Chinese seedlings by delaying the starting time of wilting point. The starting time of wilting point in the control was 3 days after discontinuation of irrigation, while in the 8, 64 and 128mM of silicate treatments were 4 days, and the 16 and 32mM treatments were 5 days. All plants were wilted after 5 days in control without irrigation whereas it showed in 8mM treatment after 6 days, in 16, 32, 64, 128mM treatments after 7 days.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.69-75
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• 2017

Dormancy-associated protein (DRM) is involved in the dormancy physiology of plants and is conserved in almost all plant species. Recent studies found that DRM genes are involved in the abiotic stress response, and characterization studies of these genes have been conducted in several plants. However, few studies have focused on DRM genes in woody plants. Therefore, in this study, cDNA coding for DRM (PagDRM1) was isolated from poplar (Populus alba ${\times}$ P. glandulosa), and its structure and expression characteristics were investigated. PagDRM1 encodes a putative protein composed of 123 amino acids, and the protein contains two conserved domains (Domain I and Domain II). PagDRM1 is present as one or two copies in the poplar genome. Its expression level was highest in the stem, followed by mature leaves, roots, and flowers. During the growth of cultured cells in suspension, PagDRM1 was highly expressed from the late-exponential phase to the stationary phase. In addition, PagDRM1 expression increased in response to drought, salt stress, and treatment with plant hormones (e.g., abscisic acid and gibberellic acid). Therefore, we suggested that PagDRM1 not only plays an important role in the induction of dormancy, but also contributes to stress tolerance in plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.238-242
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• 2003

Reclaimed tidal areas for rice cultivation are irrigated with salt mixed water when there is severe drought. Therefore, we identified the critical concentration of saline water for rice growth on a reclaimed saline soil in Korea. The experiment was conducted at the Kyehwado substation of the National Honam Agricultural Experiment Station (NHAES) during 2001-2002. Two experimental fields with 0.1-0.2% for low soil salinity and 0.3-0.4% for medium soil salinity levels were used. The experiment involved four levels of salt solution mixed with sea water (at 0.1, 0.3, 0.5, 0.7%) compared with a control using tap water in a split-plot design with three replicates. Saline solution was applied only two times at seedling stage (10 DAT and 25 DAT) for 5 days. Gyehwabyeo and dongjinbyeo, japonica rice varieties, were used in this experiment. Plant height and number of tillers sharply decreased in the 0.5% saline water in low soil salinity level and 0.1% in medium soil salinity level. For yield components, panicle number per unit area and percentage of ripened grain dramatically decreased in the 0.5% saline water in low soil salinity and 0.1% in medium soil salinity level. But 1,000-grain weight of brown rice decreased sharply in the 0.5% saline water in low soil salinity and 0.3% in medium soil salinity, indicating that this component was not much affected unlike other yield components. Milled rice yield decreased significantly with saline water level in both low and medium soil salinity. In the 0.7% low saline soil, the yield index was only 36% compared with the control. In medium soil salinity, even the control plot showed only 62% yield index compared with the control in the low soil salinity treatment. Results indicated that the critical concentration of saline water for rice growth in terms of economical income of rice production was 0.5% in low soil salinity and tap water in medium soil salinity.

• Journal of Korean Society of Forest Science
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• v.109 no.1
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• pp.31-40
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• 2020

This study aimed to investigate the effects of climate change on the survival and growth performance of Pinus densiflora and Larix kaempferi seedlings using open-field experimental warming and precipitation manipulation. We measured the survival rate, root-collar diameter, and height, and then calculated the seedling quality index (SQI) of 2-year-old seedlings under 6 treatments [2 temperatures (TC: Control; TW: Warming) × 3 precipitation manipulations (PC: Control; PD: Decreased; PI: Increased)] and performed a two-way ANOVA to test for differences.The air temperature of the warming plots was 3℃ higher than that of the control plots, while the precipitation manipulation plots received ±40% of the precipitation received by the control plots. Temperature and precipitation treatments did not significantly affect the survival rate of P. densiflora; however, the SQI of P. densiflora decreased with increasing precipitation. In contrast, the mortality rate of L. kaempferi increased with increasing temperature and decreasing precipitation. Furthermore, in L. kaempferi, TC × PI treatment resulted in the lowest SQI with a significant interaction effect observed between the two factors. In summary, low seedling production and quality should be expected in P. densiflora as precipitation increases and in L. kaempferi as temperature increases or precipitation decreases. These results indicate species-specific sensitivities to climate change of two plant species at the nursery stage. With the occurrence of global warming, the frequencies of drought and heavy rainfall events are increased, and this could affect the survival and seedling quality of tree species. Therefore, it is necessary to improve nursery techniques by establishing new adaptation strategies based on species-specific growth performance responses.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.381-388
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• 2003

The fertility of stream water in major streams of the Lake Shihwa Watershed was compared using water analyses and algal growth potential test (AGPT) in typical drought seasons from December 2001 to April 2002, The water quality varied considerably depending on streams. These streams were very rich in inorganic nutrients that the nutrient levels and characteristics of each stream could be easily determined. Through AGPT, 63.6% of growth was observed in the average values of each stream, with non-growth accounting for 36.4%. AGPT results showed that 40.9% of the 22 stations were in hypertrophic condition and 54.5% in eutrophic condition. AGPT values were significantly correlated with TIN, $NH_4$, and SRP (p <0.001); compared to other nutrients, however, they were more related to SRP and $NH_4$. Moreover, the values increased with high concentration of N and P and low N/P ratios. Nonetheless, the values were more dependent on P concentration than N concentration. This suggests that the effect of P on the water quality of lake situated in downstream may serve as a potential indicator of phytoplankton development. Depending on the drainage pattern of streams, the wastewaters of wastewater treatment plant (WwTP) and untreated wastewater (UTW) were found to have 53.4% and 46.6%, respevtively, of TIN, 51.9% and 48.1% of $NH_4$, 62.9% and 37.1% of $NO_3$, 62.6% and 37.4% of SRP, and 44.1% and 55.9% of SRSi. The AGPT value was 51.1% in WwTP wastewater and 48.9% in UTW wastewater, the concentration of WwTP wastewater was slightly higher. For untreated wastewaters flowing into the constructed wetland and into the lake, TIN accounts for 43.0% and 57.0%, respectively, of nitrogen components, $NH_4$ 44.4% and 55.6%, $NO_3$ 39.6% and 60.4%, SRP 53.5% and 46.5%, and SRSi 52.3% and 47.7%, respectively. The AGPT value was 58.0% in the constructed wetland and 42.0% in Lake Shihwa; the concentration in streams flowing into the wetland was slightly higher. Therefore, Persistent and large development of phytoplankton in Lake Shihwa cannot be prevented unless a measure tophytoplankton control is implemented. This is because the concentration of nutrients in specific streams flowing into the lake is very high, even though the inflow of water is low.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.203-203
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• 2017

The Earth's climate is rapidly changing because of increasing carbon dioxide content in atmosphere so, climate prediction models anticipate that earth surface temperature will rise by 3 to $5^{\circ}C$ in next 50 to 100 years. Therefore, frequency of un-expected weather events such as drought, salinity, low or high temperature and flooding etc. will be increasing worldwide. Furthermore, increased atmosphere temperature can influence pests and pathogens spread as well. Therefore, to protect enormous grain loss from unexpected weather conditions, studies related with combine stress conditions like abiotic plus biotic stress condition are really required. Thus, our research focused on physiological responses under combined abiotic and biotic stress condition in rice plant. To induce uniform stress condition, we used NaCl (100 mM) and salicylic acid (0.5 and 1.0 mM SA) as each stress a stimulator. Each artificial abiotic and biotic stress inducer was applied to hydroponically grown rice seedlings alone or together for four day. The data were collected in a time-dependent manner [1, 2, 3 and 4 day(s) after treatment (DAT)] and were matched with our anticipation that shoot length and shoot fresh weight was decreased in solo and combined abiotic and biotic stress condition. The lipid peroxidation content was significantly increased ($1.5{\pm}0.2$ to $2.7{\pm}0.1mg$ mg of $MDA\;g^{-1}FW$) in the first two days in both stress exposed plants, and showed the opposite trend ($0.5{\pm}0.01$ to $0.1{\pm}0.001mg$ of $MDA\;g^{-1}FW$) in last two days under multi stress condition. Superoxide dismutase (SOD) activity did not showed difference in only biotic stress condition (alone 0.5 and 1.0 mM SA) as compared to control however, it was significantly increased in multi stress condition or solo abiotic stress condition whereas, catalase (CAT), and ascorbate peroxidase (APX) activities were significantly decreased in solo biotic and combined abiotic and biotic condition. In particular, both enzymes activities were more decreased in multi stress condition as compared to solo biotic stress condition. The results for relative mRNA expression level of CAT and APX enzymes were in agreement with results of spectrophotometric values. Correlation value between each stress condition and phenotypic data showed that biotic stress condition showed high correlation with activity of CAT and APX whilst, abiotic stress condition revealed significant correlation with SOD activity.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.299-299
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• 2017

Due to the climate changes in Korea, the numbers of both torrential rain events and drought periods have increased in frequency. Water management practice against water shortage and flooding is one of the key interesting for field crop cultivation, and groundwater often serves as an important and safe source of water to crops. Therefore, the objective of this study is to evaluate the effect of groundwater table levels on soil water content and soybean development under two different textured soils. The experiment was conducted using lysimeter located in Miryang, Korea. Two types of soils (sandy-loam and silty-loam) were used with three groundwater table levels (0.2, 0.4, 0.6m). Mean soil water content during the soybean growth period was significantly influenced by groundwater table levels. With the continuous groundwater level at 0.2m from the soil surface, soil water content was not statistically changed between vegetative and reproductive stage, but the 0.4 and 0.6m groundwater table level was significantly decreased. Lower chlorophyll content in soybean leaves was found in shallow water table treatment in earlier part of the growing season, but the chlorophyll contents were non-significant among water table treatments. Groundwater table level treatments were significantly influenced on plant available nitrogen content in surface soil. The highest N contents were observed in 0.6m groundwater table level. It is probably due to the nitrogen loss by denitrification as the result of high soil water content. The length and dry weight of primary root was influenced by groundwater level and thus the highest length and dry weight of root were observed in 0.6m water table level. This result showed that soybean root growth did not extend below the groundwater level and increased with the depth of groundwater table level. The results of this study show that the management of groundwater level can influence on soil characteristics, especially on soil water content, and it is an important practice of to reduce yield loss caused by the water stress during the crop growing season.