Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation and Expression of Dormancy-associated protein 1 (DRM1) in Poplar (Populus alba × P. glandulosa)

현사시나무에서 Dormancy-associated protein 1 (DRM1) 유전자의 분리와 발현특성 구명

  • Yoon, Seo-Kyung (Department of Forest Genetic Resources, National Institute of Forest Science) ;
  • Bae, Eun-Kyung (Department of Forest Genetic Resources, National Institute of Forest Science) ;
  • Choi, Hyunmo (Department of Forest Genetic Resources, National Institute of Forest Science) ;
  • Choi, Young-Im (Department of Forest Genetic Resources, National Institute of Forest Science) ;
  • Lee, Hyoshin (Department of Forest Genetic Resources, National Institute of Forest Science)
  • 윤서경 (국립산림과학원 산림유전자원부) ;
  • 배은경 (국립산림과학원 산림유전자원부) ;
  • 최현모 (국립산림과학원 산림유전자원부) ;
  • 최영임 (국립산림과학원 산림유전자원부) ;
  • 이효신 (국립산림과학원 산림유전자원부)
  • Received : 2017.02.01
  • Accepted : 2017.03.17
  • Published : 2017.03.31
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Abstract

Dormancy-associated protein (DRM) is involved in the dormancy physiology of plants and is conserved in almost all plant species. Recent studies found that DRM genes are involved in the abiotic stress response, and characterization studies of these genes have been conducted in several plants. However, few studies have focused on DRM genes in woody plants. Therefore, in this study, cDNA coding for DRM (PagDRM1) was isolated from poplar (Populus alba ${\times}$ P. glandulosa), and its structure and expression characteristics were investigated. PagDRM1 encodes a putative protein composed of 123 amino acids, and the protein contains two conserved domains (Domain I and Domain II). PagDRM1 is present as one or two copies in the poplar genome. Its expression level was highest in the stem, followed by mature leaves, roots, and flowers. During the growth of cultured cells in suspension, PagDRM1 was highly expressed from the late-exponential phase to the stationary phase. In addition, PagDRM1 expression increased in response to drought, salt stress, and treatment with plant hormones (e.g., abscisic acid and gibberellic acid). Therefore, we suggested that PagDRM1 not only plays an important role in the induction of dormancy, but also contributes to stress tolerance in plants.

Dormancy-associated protein (DRM)은 식물의 휴면생리에 관여하는 대표적인 단백질로 거의 모든 식물에 보존되어 있다. 최근 DRM 유전자가 비생물적 스트레스 반응에 관여하는 것으로 알려지면서 이 유전자의 특성구명 연구가 여러 식물에서 이루어지고 있다. 그러나 아직까지 나무에서는 DRM 유전자에 대한 연구가 거의 없는 실정이다. 따라서 본 연구에서는 DRM 유전자를 현사시나무(Populus alba ${\times}$ P. glandulosa)에서 분리하여 이를 PagDRM1이라 명명하고, 유전자의 구조와 발현특성을 조사하였다. PagDRM1 유전자는 123개의 아미노산으로 구성된 단백질을 암호화하며, 2개의 영역(Domain I과 Domain II)이 잘 보존되어 있다. PagDRM1은 현사시나무의 염색체에 1 ~ 2 copy가 존재하며, 줄기에서 가장 높게 발현하였고 성숙 잎, 뿌리 및 꽃에서도 높은 발현 수준을 나타내었다. 현탁배양세포의 생장주기에서는 늦은 지수생장기부터 정지기까지 높게 발현하였다. 또한 PagDRM1은 건조와 염 스트레스에 반응하여 발현이 증가하는 것으로 나타났다. 식물호르몬 처리에 의해서는 ABA와 GA 처리에 의한 발현 증가를 나타내었다. 따라서 PagDRM1은 식물의 휴면유도 과정에서 중요한 역할을 할 뿐만 아니라 환경 스트레스 내성에도 기여하는 것으로 생각된다.

Keywords

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