• 한국자원식물학회지
• /
• 제30권5호
• /
• pp.571-577
• /
• 2017

ABA는 식물에서 비 생물학적 스트레스 내성에 관여하는 중요한 식물 호르몬이다. 애기장대의 group A bZIP 전사인자는 ABA 신호전달 과정에 중요한 역할을 한다고 알려져 있다. 그러나 벼에서는 group A bZIP 전사인자의 기능이 잘 알려져 있지 않다. 따라서 우리는 벼에서 group A bZIP 전사인자인 OsABF3(Oryza sativa ABA responsive element binding factor 3)를 연구하였다. 이를 위해 벼의 다양한 조직과 다양한 스트레스(가뭄, 염분, 저온, ABA, 산화 스트레스)에 따른OsABF3발현 패턴을 분석하였다. 또한 maize의 원형질체에서 GFP fusion 벡터를 사용한 세포 내 위치 분석을 통해 OsABF3가 핵단백질이라는 것을 확인하였다. Yeast one-hybrid 실험을 통해 OsABF3의 C-terminal 부분이 ABREs에 결합한다는 것과 N-terminal 부분이 하위 유전자의 transactivation 하는데 필요하다는 것을 알 수 있었다. 그리고 T-DNA가 삽입된 OsABF3의 homozygous 돌연변이체가 야생형과 과발현체에 비해 발아와 발아 후 단계에서 고농도의 ABA에 대한 민감도가 더 감소한 것을 알 수 있었다. 결과적으로 종합해 볼 때 OsABF3는 ABA의 의존적인 경로를 통해 비 생물학적 스트레스에 반응하는 유전자의 발현을 조절하는 기능을 하는 전사 조절자이다. 또한 OsABF3의 transactivation을 측정하는 실험에 있어서 억제 domain이 존재한다는 결과를 얻었다.

• 식물병연구
• /
• 제24권3호
• /
• pp.221-232
• /
• 2018

근권세균은 식물 생육과 건강 증진에 중요한 역할을 하며 생물학적 스트레스뿐만 아니라 저온, 고온, 건조 및 염과 같은 비생물적 스트레스에도 내성을 부여한다. 본 연구는 토마토에 생물적 및 비생물적 스트레스를 완화시키는 기능을 가진 식물생장촉진 근권세균(plant growth promoting rhizobacteria, PGPR)을 선발하는 것을 목표로 하였으며 토마토 근권에서 Variovorax sp. PMC12균주를 분리하였다. PMC12균주는 in vitro에서 PGPR의 특성으로 알려진 암모니아, IAA, 시드로포아 및 ACC 탈아민효소를 생성하였다. PMC12 균주를 처리한 토마토는 대조구에 비해 염, 저온 및 건조 스트레스 조건에서 지상부 생체중이 유의적으로 높았다. 또한 PMC12 균주를 처리한 토마토는 Ralstonia solanacearum에 의한 세균성 시들음병에 대한 저항성이 증가되었다. 결과적으로 PMC12 균주는 식물의 비생물적 스트레스 및 생물적 스트레스에 대한 감수성을 감소시키는 유망한 생물학적 방제제 및 생물활성제로 사용될 수 있을 것으로 전망된다.

• Journal of Plant Biotechnology
• /
• 제44권1호
• /
• pp.82-88
• /
• 2017

유전자총을 이용한 형질전환 체계와 PPT (D-L-phosphinothricin) 선발을 통하여 나리 인편조직으로부터 형질전환 식물체가 획득되었다. 본 연구에서 나리 '레드플레임' 품종의 인편조직에 선발유전자로 제초제저항성 유전자인 bar 유전자 그리고 내염성과 내건성의 복합환경저항성을 나타내는 AtSIZ 유전자를 목적유전자로 가지고 있는 플라스미드를 금입자에 코팅해서 유전자총을 이용해서 형질전환 하였다. 이러한 형질전환 체계 확립을 위해 헬륨가스 압력은 1,100 psi, 금 입자크기는 $1.0{\mu}m$ 그리고 목적 절편체까지의 거리는 6 cm 그리고 유전자총 처리 24시간 전과 후에 0.2 M sorbitol과 0.2 M mannitol을 혼합해서 MS배지에 첨가한 프로토콜로부터 우수한 형질전환 결과를 나타내었다. 유전자총 발사 처리 후, 1주간 선발제로 사용되는 PPT가 없는 MS 배지로 이식하여 배양 후, PPT 10 mg/l이 첨가된 선발배지에서 4주 간격의 계대배양을 통해 8-12주간 선발과정을 거친다. PPT 선발 배지에서 생존한 신초가 형성된 형질전환 나리 인편 조직들을 호르몬이 없는 MS 배지로 다시 옮겨주면 발근 및 추가 생육이 이루어진다. 생존한 형질전환 나리 기내 소식물체들로부터 PCR 검정을 통해 선발유전자인 bar 유전자 그리고 목적유전자인 AtSIZ 유전자의 도입이 확인되었다. 결론적으로 100여개의 나리 인편조직을 본 연구에서 확립된 유전자총 실험프로토콜을 이용하면 대략적으로 형질전환 나리 17-18 개체를 획득할 수 있으며 본 연구에 기술된 유전자 총 매개 형질전환 체계는 추가적인 보완이 이루어지면, 향후 나리 육종 프로그램에 기여할 것이다.

• Ecology and Resilient Infrastructure
• /
• 제6권3호
• /
• pp.163-170
• /
• 2019

신설 제방의 사면 안정화를 위해 사용하는 토목 자재는 완공 이후 유입되는 식생의 종류 및 생장에 큰 영향을 미친다. 최근 미생물 유래 베타글루칸과 잔탄검 계열 바이오폴리머가 식물의 생육을 촉진시킬 수 있는 새로운 친환경 제방 사면 안정화 물질로 주목 받고 있다. 본 연구는 바이오폴리머 신소재의 생태성 평가를 목적으로, 특히 생태계 내에서 다수를 차지하는 우점 식물종들이 생태계의 특성을 크게 좌우하는 점에 주목하여, 바이오폴리머 신소재가 국내 하천에서 흔히 발견되는 자생 초본 식물 8종과 녹화용 식물 2종의 발아 및 생육에 미치는 영향을 시험하였다. 시험 식물종들의 종자 발아율은 바이오폴리머가 혼합된 토양의 영향을 받지 않았다. 반면 식물 생육은 바이오폴리머가 혼합된 토양에서 증가하였는데, 특히 돌피의 경우 약 2배정도의 총 생물량이 증가하였다. 식물 뿌리의 생육이 증가하는 경향을 보였고, 잎의 건중량 대비 엽면적이 증가하였다. 뿌리의 생육 증진과 잎의 건중량 대비 엽면적 증가가 환경 스트레스에 적응된 식물종들의 대표적 기능 형질인 점을 감안하면, 제방 사면에 처리된 바이오폴리머 신소재는 국내 하천 우점종들의 생육 및 환경 스트레스 저항성을 높일 것으로 예측된다.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.77.2-78
• /
• 2003

An EREBP/AP2-type transcription factor (CaPFl) was isolated by DDRT-PCR following inoculation of soybean pustule pathogen Xanthomonas axonopodis pv. glycines Bra which induces HR on pepper leaves. Genomic Southern blot analysis revealed that the CaPFl gene is present as a single copy within the hot pepper genome. The deduced amino acid sequence of CaPFl has two potential nuclear localization signals, a possible acidic activation domain, and an EREBP/AP2 motif that could bind to a conserved cis- element present in promoter region of many stress-induced genes. The mRNA level of CaPFl was induced by both biotic and abiotic stresses. We observed higher-level transcripts in resistance-induced pepper tissues than diseased tissues. Expression of CaPFl is also induced upon various abiotic stresses including ethephon, MeJA, cold stress, drought stress and salt stress treatments. To study the role of CPFI in plant, transgenic Arabidopsis and tobacco plants which express higher level of pepper CaPFl were generated. Global gene expression analysis of transgenic Arabidopsis by cDNA microarray indicated that expression of CaPFl in transgenic plants affect the expression of quite a few GCC box and DRE/CRT box-containing genes. Furthermore, the transgenic Arabidopsis and tobacco plant, expressing CaPFl showed tolerance against freezing temperature and enhanced resistance to Pseudomonas syrnigae pv. tabaci. Taken together, these results indicated that CaPFl is a novel EREBP/AP2 transcription factor in hot pepper plant and it may has a significant role(s) in regulation of biotic and abiotic stresses in plant.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.74-74
• /
• 2018

Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. This study was to develop highly polymorphic EST-SSR markers based on cross-species transferability of derived SSRs from switchgrass EST databases and characterize newly developed EST - SSRs to better understand the genetic diversity of collected 37 germplasm accessions of little millet. A total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST - SSR markers were developed based on the trials of transferability of these primers in little millet. The EST - SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. T he average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. T he polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST - SSR markers in assessing genomic relationships in little millet. T he findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.

• 농업과학연구
• /
• 제46권4호
• /
• pp.769-779
• /
• 2019

Chilli peppers are predominantly cultivated in open field systems under abiotic and biotic stress conditions. Abiotic and biotic factors have a considerable effect on plant performance, fruit quantity, and quality. Chilli peppers grow well in a tropical climate due to their adaptation to warm and humid regions with temperatures ranging from 18 to 30℃. Nowadays, chilli peppers are cultivated all around the world under different climatic conditions, and their production is gradually expanding. Expected climate changes will likely cause huge and complex ecological consequences; high temperature, heavy rainfall, and drought have adverse effects on the vegetative and generative development of all agricultural crops including chilli peppers. To gain better insight into the effect of climate change, the growth, photosynthetic traits, morphological and physiological characteristics, yield, and fruit parameters of chilli peppers need to be studied under simulated climate change conditions. Moreover, it is important to develop alternative agrotechnologies to maintain the sustainability of pepper production. There are many conceivable ideas and concepts to sustain crop production under the extreme conditions of future climate change scenarios. Therefore, this review provides an overview of the adverse impacts of climate change and discusses how to find the best solutions to obtain a stable chilli pepper yield.

• Journal of Forest and Environmental Science
• /
• 제33권2호
• /
• pp.97-104
• /
• 2017

Investigation was carried out on response of Tetrapleura tetraptera (Schum. and Thonn.) to soil, water and light with the view of its domestication and introduction to different ecological regions. The experiment was arranged in a factorial experiment of $3{\times}3{\times}3$ in a completely randomized design (CRD) with three replicates. The factors were: soil textural class (Loamy sand, Sand and Sandy clay loam), watering regime (daily, twice a week and once a week) and light intensity (100%, 75% and 50%). Soil textural classes had significant influence on collar diameter, stem height, number of leaflets, root/shoot ratio and relative growth rate of Tetrapleura seedlings. Seedlings grown on loamy sand recorded the highest mean value- 2.28 mm for collar diameter, stem height- 12.9 cm, number of leaflets- 19.9, chlorophyll b- $0.34mg\;mL^{-1}$, leaf relative water content- 27.4% and relative growth rate- $0.037mg\;g^{-1}\;day^{-1}$. Watering regime had significant influence on the collar diameter of Tetrapleura. Seedlings watered daily recorded the highest mean value- 2.25 mm for collar diameter. Light intensity significantly influenced collar diameter and root/shoot ratio. Seedlings exposed to 100% light intensity recorded higher mean value for collar diameter- 2.28 mm and root/shoot ratio- 1.481 cm. The interaction between soil textural class and light intensity significantly affected collar diameter, stem height and number of leaflets. Higher mean value for collar diameter (2.47 mm) stem height (13.25 cm) and number of leaflets (21.16) were recorded while the interaction between soil textural class, light intensity and watering regime was significant for only number of leaflets. Tetrapleura exhibited some level of tolerance to different soil texture, drought and light intensity. Therefore, Tetrapleura has the potentials to be raised in different ecological zones characterized by difference in soil, rainfall and amount of sunshine.

• 한국환경과학회지
• /
• 제23권7호
• /
• pp.1299-1305
• /
• 2014

For evaluating the effect of various artificial planting soil properties on the Euonymus fortunei 'Emelad'n Gold' growth, a container green wall system experiment was conducted in a wall of greenhouse at Konkuk University, Glocal campus. The experimental artificial planting grounds were prepared with different organic soil conditioner ratios (Control, $A_4O_1$, $A_2O_1$ and $A_1O_1$) and with drought tolerance and an ornamental value Euonymus fortunei 'Emelad'n Gold' was planted. The soil and plant characteristics were investigated from April to Jun 2010. The volumetric soil moisture contents were significantly increasing order as the amount of organic soil conditioner level increased in order to $A_1O_1$ > $A_2O_1$ > $A_4O_1$ > Control. At 4 treatment, soil chemical properties were inversely related to organic soil container ratios increase. The differences of root collar caliper, number of branch, and survival rate between the organic soil conditioner ratio were not significantly affected by organic soil conditioner. But, plant height, internode length, leaf length and leaf width were significantly shorter on plants planted $A_1O_1$ than plants planted other treatments. Therefore, Euonymus fortunei 'Emelad'n Gold' had good growth response regardless of organic soil conditioner ratio and the plant is expected to be a highly valuable shrub for the green wall system if it should be considered in integration with stormwater retention or as a soil conditioner for increasing soil water contents in artificial planting soil.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.149-149
• /
• 2017

The RING (Really Interesting New Gene) finger proteins are known to play crucial roles in various abiotic stresses in plants. In this study, we report on RING finger E3 ligase, ${\underline{O}ryza}$ ${\underline{s}ativa}$ ${\underline{s}alt$-, ${\underline{A}BA}$- and ${\underline{d}rounght}$ stress-${\underline{i}nduced}$ RING finger ${\underline{p}}rotein{\underline{1}}$ gene (OsSAD1). In vitro ubiquitination assay demonstrated that unlike OsSAD1, a single amino acid substitution ($OsSAD1^{C168A}$) of the RING domain showed no E3 ligase activity, supporting the notion that the activity of most E3s is specified by a RING domain. Result of Yeast-Two hybridization, In vivo protein degradation assay supports that OsSAD1 interacting with 3 substrate, OsSNAC2, OsGRAS44 and OsPIRIN1, and mediates proteolysis of 3 substrates via the 26S proteasome pathway. Subcellular localizations of OsSAD1 while approximately 62% of transient signals were detected in cytosol, 38% of signals were showed nucleus. However, transiently expression of OsSAD1 was detected in cytosol 30% while as 70% of nucleus under 200 mM salt treated rice protoplasts. Results of bimolecular fluorescence complementation (BiFC) showed that two nucleus-localized proteins (OsSNAC2 and OsGRAS44) interacted with OsSAD1 in the both cytosol and nucleus. Heterogeneous overexpression of OsSAD1 Heterogeneous overexpresssion of OsSAD1 in Arabidopsis exhibited sensitive phenotypes with respect to Salt-, mannitol-responsive seed germination, seedling growth. In ABA conditions, OsSAD1 overexpression plants showed highly tolerance phenotypes, such as root length and stomatal closure. Our findings suggest that the OsSAD1 may play a negative regulator in salt stress response by modulating levels of its target proteins.