• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.967-987
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• 1996

The present study was to evaluate the healing patterns of guided tissue regeneration( GTR) using resorbable $Vicryl^{(R)}$(polyglactin 910) mesh and nonresorbable expanded polytetrafluoroethylene(ePTFE) membrane with or without bone grafting using autogeneous bone and demineralized freeze-dried bone allograft(DFDBA) in the grade II furcation defects. Mucoperiosteal flaps were reflected buccally in the mandibular 2nd, 3rd and 4th premolar areas and furcation defects were created surgically by removing $5{\times}6mm$ alveolar bone in 4 dogs. Root surfaces were thoroughly debrided of periodontal ligament and cementum, and notches were placed on root surface at the most apical bone level. In the right and left mandibular quadrant, each tooth was received $Vicryl^{(R)}$ mesh(ACE Surgical Supply Co., USA) only, $Vicryl^{(R)}$ mesh with DFDBA, $Vicryl^{(R)}$ mesh with autogeneous bone grafts, ePTFE membrane($Core-tex^{(R)}$ membrane, W.L. Gore & Associates Inc., USA) only, ePTFE membrane with DFDBA or ePTFE membrane with autogeneous bone grafts. For the fluorescent microscopic examination, fluorescent agents were injected at 2, 4 and 8 weeks after surgery. Four weeks after surgery, 2 dogs were sacrificed and ePTFE membranes were removed from remaining 2 dogs, which were sacrificed at 12 weeks after surgery. Undecalcified tissues were embedded in methylmethacrylate and $10{\mu}m$ thick sections were cut in a buccolingual direction. These sections were stained with hematoxylin-eosin stain and Masson's trichrome stain, and evaluated by descriptive histology and linear measurements. The results were as follows : 1) $Vicryl^{(R)}$ mesh group showed less connective tissue attachment than ePTFE membrane group. 2) The combination of GTR using $Vicryl^{(R)}$ mesh and osseous grafts resulted in new attachment and new bone formation more than GTR using $Vicryl^{(R)}$ mesh only. 3) GTR using ePTFE membrane, with or without osseous grafts, enhanced periodontal regeneration. 4) Root resorption and dentoalveolar ankylosis were observed in the areas treated with the combination of GTR and DFDBA. It was suggested that the effect of adjunctive bone grafting in GTR procedure depends on the materials and the physical properties of barrier membranes. $Vicryl^{(R)}$ mesh performed a barrier function and the use of adjunctive bone grafting may enhance the periodontal regeneration.

• Journal of Life Science
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• v.25 no.11
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• pp.1298-1303
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• 2015

Machilus thunbergii (Lauraceae) is an evergreen tree cultivated in Korea and Japan. M. thunbergii has long been used as a traditional medicine in Korea, China, and Japan to treat various diseases, including edema, abdominal pain, and abdominal distension. In this study, dried stem bark of M. thunbergii extracted in methanol and extract was partitioned into n-hexane, CHCl₃, and BuOH. The CHCl₃-soluble extracts chromatographed on silica gel column using a CHCl₃/acetone and n-hexane/EtOAc mixture to afford Compound 1 and 2. Two dibenzylbutane lignans, macelignan (1) and meso-dihydroguaiaretic acid (2), were isolated from the CHCl₃-soluble extract of M. thunbergii stem bark. The structures of 1 and 2 were determined by ¹H- and ¹³C-NMR spectroscopic data analyses and a comparison with literature data. The tyrosinase inhibitory activity of the isolated compounds was evaluated. Among these compounds, Compound 2 strongly inhibited the monophenolase (IC₅₀=10.2 μM) activity of tyrosinase. A kinetic analysis showed that Compound 2 was a competitive inhibitor. The apparent inhibition constant (K_i) for Compound 2 binding to free enzyme was 4.8 μM. Based on these results, it can be concluded that meso-dihydroguaiaretic acid (2) is a potential candidate for the treatment of melanin biosynthesis-related skin diseases.

• The Plant Pathology Journal
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• v.33 no.3
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• pp.337-344
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• 2017

To develop a commercial product using the mycoparasitic fungus Simplicillium lamellicola BCP, the scale-up of conidia production from a 5-l jar to a 5,000-l pilot bioreactor, optimization of the freeze-drying of the fermentation broth, and preparation of a wettable powder-type formulation were performed. Then, its disease control efficacy was evaluated against gray mold diseases of tomato and ginseng plants in field conditions. The final conidial yields of S. lamellicola BCP were $3.3{\times}10^9conidia/ml$ for a 5-l jar, $3.5{\times}10^9conidia/ml$ for a 500-l pilot vessel, and $3.1{\times}10^9conidia/ml$ for a 5,000-l pilot bioreactor. The conidial yield in the 5,000-l pilot bioreactor was comparable to that in the 5-l jar and 500-l pilot vessel. On the other hand, the highest conidial viability of 86% was obtained by the freeze-drying method using an additive combination of lactose, trehalose, soybean meal, and glycerin. Using the freeze-dried sample, a wettable powder-type formulation (active ingredient 10%; BCP-WP10) was prepared. A conidial viability of more than 50% was maintained in BCP-WP10 until 22 weeks for storage at $40^{\circ}C$. BCP-WP10 effectively suppressed the development of gray mold disease on tomato with control efficacies of 64.7% and 82.6% at 500- and 250-fold dilutions, respectively. It also reduced the incidence of gray mold on ginseng by 65.6% and 81.3% at 500- and 250-fold dilutions, respectively. The results indicated that the new microbial fungicide BCP-WP10 can be used widely to control gray mold diseases of various crops including tomato and ginseng.

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.4
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• pp.86-92
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• 2000

This study was conducted to determine the optimal mixing ratio of the paper mill sludge(PMS) and pig manure(PM). Since the former contains lots of total carbon and low nitrogen, it was used as carbon source. Also, dried paper mill sludge(DPMS) was added to the mixture to control the water content. The treatments was composed of four as follows, PMS-100(PM 0%+PMS 80%+DPMS 20%), PMS-85(15+65+20), PMS-70(30+50+20), and PMS-55(45+35+20). The mixtures were composted under aerobic condition in $1.25m^3$ static piles. The piles were aerated for 15 minutes per day and turned over the mixture once a week at the early stage of composting. To estimate the maturity of composts, the changes of physico-chemical properties such as temperature, pH, C/N ratio and color were monitored every week. The 25-30 and 55-60% as optimal condition of C/N ratio and moisture content were respectively recommended for effective composting by the evaluation of the changes of phsico-chemical properties for materials taken from compost files during the composting period. When the 30 and 45% of PM were mixed with PMS, the maturity time at least demanded to the stable state were shortened and the qualify of the final product was improved in a view of nutritional components.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.2
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• pp.323-329
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• 1995

For the effective utilization of dark meat separated as by-product from skip-jack canning, the dark meat concentrate(DPC) was prepared by removal of extractable materials with ethanol from dried dark meat. Dark meat protein hydrolysate(DPH) was prepared by the hydrolysis of DPC with ${\alpha}-chymotrypsin$. ${\alpha}-Chymotrypsin$ hydrolysed DPC to the extend of 79% during 10hr. The solubility over a pH range 1~12 showed similar trend on the both of DPH and DPC. The highest solubility was 81% on the DPH and was 36% on the DPC at pH 3. The lowest solubility was 65% on the DPH and was 22% on the DPC at pH 7. The content of total free amino acid was higher in the DPC than in the DPH, but the content of total essential free amino acid was higher in the DPH. Especially, the contents of taurine in the DPC and DPH were much higher than those of other amino acids. The result of sensory evaluation on the fish sauce analogue showed good taste, color and odor at the supplemented level of 8g DPH per 100ml of raw solution of fish sauce analogue and didn't show signifcaint difference compared with market fish sauce(p<0.05). On the preparation of surimi gel, 2% substitution of DPH for the supplemented starch was the most appropriate level.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10613-10619
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• 2015

Background: Both alcohol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, the dried root tuber of which is named Baifuzi in Chinese, have been used for folklore treatment of cancer in Northeast of China. However, little is known about which is most suitable to the cancer therapy. Materials and Methods: Serum pharmacology and MTT assays were adopted to detect the effects of ethanol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, prepared by heat reflux methods, on proliferation of different cancer cells. Results: Cancer cells treated with medium supplemented with 10%, 20%, 40% serum(v/v) containing ethanol extract had a decline in viability, with inhibition rates of 7.69%, 21.8%, 41.9% in MCF-7 cells, 42.8%, 48.1%, 51.8% in SGC-7901 cells, 44.1%, 49.2%, 53.7% in SMMC-7721 cells, 6.8%, 15.2%, 39.8% in HepG2 cells, 7.57%, 16.3%, 36.2% in HeLa cells, 6.24%, 12.5%, 27.4% in A549 cells, and 7.20%, 17.5%, 31.3% in MDA-MB-231 cells, respectively. Viability in the aqueous extract groups was no different with that of controls. Conclusions: An ethanol extract of Sauromatum giganteum(Engl.) Cusimano and Hett inhibited the proliferation of SMMC-7721, SGC-7901 and MCF-7 cells, which supports the use of alcoholic but not aqueous extracts for control of sensive cancers, which might include hepatocarcinoma, gastric cancer and breast cancer.

• The korean journal of orthodontics
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• v.26 no.3
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• pp.309-316
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• 1996

The purpose of this study was to determine the effect of salivary contamination of etched enamel on shear bond strength of a bracket adhered to etched enamel. Eighty extracted human permanent premolars were used in this study. These samples were divided into two groups. Buccal surface of samples were etched in vitro with 38% phosphoric acid for 15 seconds and 60 seconds. Each group was divided into four subgroups. Etched enamel surfaces were contaminated with saliva for 0, 1, 20, 60 seconds, washed and dried. Test surfaces were examined using scanning electron microscope(SEM). The shear bond strength of each sample was determined with a universal testing instrument(Instron Co. Model 4201). Results were as follows; 1. Salivary contamination for 1, 20, 60 seconds did not affect shear bond strength when compared with the uncontaminated enamel group. 2 There was no significant difference(P>.05) in shear bond strength between 15 sec. and 60 sec. etching in uncontaminated enamel groups. 3. When samples were examined using SEM, organic materials coated enamel surface masked the etched pattern partially.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1572-1578
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• 2005

Response surface methodology was used for monitoring changes in physicochemical properties with heating condition of ginseng which was sliced and freeze-dried. As heating temperature and time increased, soluble solid content decreased and browning color increased. Also, acidic polysaccharide and total phenolics increased with the increase in heating temperature and time. Heating condition for maximum soluble solid content was 146.05$^{\circ}C$ in heating temperature and 18.16 min in heating time. Maximum value of crude saponin content was 64.40 mg/g in 160.00$^{\circ}C$ and 20.00 min. Crude saponin content was influenced by heating time but the other properties were influenced by heating temperature.

• Journal of Korean Society of Forest Science
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• v.63 no.1
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• pp.61-68
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• 1984

The oak-mushroom is one of the special minor products utilizing hardwoods, such as oaks in the mountaineous villages, as a food for health and export in Korea. However, oak-mushroom farmers have been faced the problems such as small cultivation scale, unskilled technique of production, management of cultivation, and low productivity. It is now a turning point presenting the optimum and reasonable management techniques to increase the productivity with consideration of balanced income and expenditure. This report analyzed the investment effectiveness of cultivation. The financial rate of return (FRR) of oak-mushroom cultivation is as high as 10.3 percent compared with that of other farmings. Moreover, the FRR remarkably increased with increased cultivation scales. The FRR could be highly improved if the price of both mushroom-logs and dried mushroom products, is stable and the cultivation techniques for higher productivity are developed. The policy providing the constant price of raw materials and mushroom products, and new advanced cultivation techniques enable the enhancement of investment for the oak-mushroom cultivation.

• Composites Research
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• v.31 no.1
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• pp.1-7
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• 2018

In the case of a conventional composite patch repair using a heat blanket, the adhesive is pressurized using only a vacuum bag. In this study, however, a pressurization system has been developed to apply additional air pressure on the vacuum bag. In order to verify the performance of the developed system, the composite laminates were repaired with scarf patches and then tested under tensile load to be compared with the strength of the defect-free laminate. Tensile tests were also conducted on specimens with the same configuration but bonded in an autoclave. As a result of the test, the tensile strengths of the specimens repaired using the heat blanket with vacuum only without external pressure, the specimens repaired with additional pressure by the developed system, and the specimens repaired with the same external pressure in an autoclave, showed the strength recovery ratios of 74.9, 81.0, and 78.2%, respectively. The results of the tensile test after moisture saturation and the dried fatigue test also showed that the strength recovery ratios of the specimens repaired under the external pressure of 1 atm using the developed system are slightly higher than that of specimens bonded in autoclave.