• Title/Summary/Keyword: Down-sampling

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Optimization of monitoring methods for air-borne bacteria in the environmental conditions of pig facilities (무균 돈사 환경 모니터링을 위한 대기 중 미생물 탐지기법 확립)

  • Lee, Deok-Yong;Seo, Yeon-Soo;Kang, Sang-Gyun;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.46 no.3
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    • pp.255-261
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    • 2006
  • Experimental animals have been used to biological and medical purposes and the animals must be, for these purposes, healthy and clean to microbial infection. However, the animals can be easily exposed to pathogenic microorganism via several routes. Of the routes, environmental conditions are the most important factors to keep the animals healthy and clean, especially air condition. Monitoring of air-condition has been required to keep the animal healthy and clean. However, any guideline is not available for experimental conditions with pigs. Therefore, the sampling times and points were compared in different conditions to establish an optimal protocol for monitoring of air borne bacteria. Tryptic soy agar(TSA), blood agar containing 5% defibrinated sheep blood and Sabraud dextrose agar(SDA) were used as media to capture total bacteria, pathogenic bacteria and fungi, respectively. Two methods, compulsive capture using an air-sampler and capturing fall-down bacteria were used to capture the microorganisms in the air. The points and time of capturing were different at each experiment. Air borne microorganisms were captured at three and five points in the open and closed equipments, respectively. Air was collected using an air-sampler for 1 min and 5 min and the agar plates as open status were left from 30 min to 2hr. At first, we monitored an experimental laboratory which dealt with several pathogenic bacteria and then, a protocol obtained from the investigation was applied to open or close experimental conditions with pigs. Number of bacteria was high from 10:00 to 15:00, especially on 13:30-15:30 but sharply decreased after 17:00. The tendency of the number of bacteria was similar between two methods even though the absolute number was higher with air sampler. Critical difference in the number of cells was observed at 5 min with air sampler and 2 hr with fall-down capturing method. However, 1 min with air sampler and 1 hr with fall-down capturing were the best condition to identify bacterial species collected from the air. Number of bacteria were different depending on the sampling points in closed condition but not in opened condition. Based on our results, a guide-line was suggested for screening air-borne microorganism in the experimental conditions with pigs.

Analysis of Pollutant Characteristics in Nakdong River using Confirmatory Factor Modeling (확인적 요인모형을 이용한 낙동강 유역의 오염특성 분석)

  • Kim, Mi-Ah;Kang, Taegu;Lee, Hyuk;Shin, Yuna;Kim, Kyunghyun
    • Journal of Korean Society on Water Environment
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    • v.28 no.1
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    • pp.84-93
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    • 2012
  • The study was conducted to analyze the spatio-temporal changes in water quality of the major 36 sampling stations of Nakdong River, depending on each station, season using the 17 water quality variables from 2000 to 2010. The result was verified to interpret the characteristics of water quality variables in a more accurate manners. According to the Principal component analysis (PCA) and Exploratory factor analysis (EFA) results; the results of these analyses were identified 4 factors, Factor 1 (nutrients) included the concentrations of T-N, T-P, $NO_{3}-N$, $PO_{4}-P$, DTN, DTP for sampling station and season, Factor 2 (organic pollutants) included the concentrations of BOD, COD, Chl-a, Factor 3 (microbes) included the concentrations of F.Coli, T.Coli, and Factor 4 (others) included the concentrations of pH, DO. The results of a Cluster analysis indicated that Geumhogang 6 was the most contaminated site, while tributaries and most of the down stream sites of Nakdong River were mainly affected by each nutrients (Factor 1) and organic pollutants (Factor 2). The verification consequence of Confirmatory factor analysis (CFA) from Exploratory factor analysis (EFA) result can be summarized as follows: we could find additional relations between variables besides the structure from EFA, which we obtained through the second-order final modeling adopted in CFA. Nutrients had the biggest impact on water pollution for each sampling station and season. In particular, It was analyzed that P-series pollutant should be controlled during spring and winter and N-series pollutant should be controlled during summer and fall.

Characteristics of Water and Environmental Qualities of Seho Watershed in Suwon City (수원시 서호천의 수질현황 및 환경질 특성)

  • Chi, Hong-Jin;Lee, Sang-Eun;Choi, Young-Keun;Lee, Jae-Dong
    • Journal of Environmental Science International
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    • v.22 no.6
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    • pp.733-744
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    • 2013
  • This study was to investigate characteristics of Seoho watershed in Suwon city. $BOD_5$ and SS were selected due to the one of the important factors of the water qualities. Monitoring was conducted monthly for four years during the non-rainfall time. Also, we have been monitored $BOD_5$, $COD_{Mn}$, SS, TN and TP with two times sampling after the rainfalls. The highest concentrations of $BOD_5$ and SS were observed in downstream compare with upstream and midstream during the non-rainfall time. No change was observed in $BOD_5$ and $COD_{Mn}$ during the non-rainfall time and after the rainfalls. The monitoring result indicated that the concentration of SS was the highest in downstream after the rainfalls. We have collected the samples two times after the rainfalls. The rainfall intensity in first sampling was two times higher than second sampling. TN and TP concentrations were increased with increasing the rainfall intensity at all stream. The ESB (Ecological Score of Benthic macroinverterbrate community) index was used to evaluate the statement of stream. ESB results were identified that the upstream is protected waters and the down and midstream is reformed waters. EBS analysis results indicated that the Seoho watershed was ${\beta}$-mesosaprobic at all stream.

Sigma Delta Decimation Filter Design for High Resolution Audio Based on Low Power Techniques (저전력 기법을 사용한 고해상도 오디오용 Sigma Delta Decimation Filter 설계)

  • Au, Huynh Hai;Kim, SoYoung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.49 no.11
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    • pp.141-148
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    • 2012
  • A design of a 32-bit fourth-stage decimation filter decimation filter used in sigma-delta analog-to-digital (A/D) converter is proposed in this work. A four-stage decimation filter with down-sampling factor of 512 and 32-bit output is developed. A multi-stage cascaded integrator-comb (CIC) filter, which reduces the sampling rate by 64, is used in the first stage. Three half-band FIR filters are used after the CIC filter, each of which reduces the sampling rate by two. The pipeline structure is applied in the CIC filter to reduce the power consumption of the CIC. The Canonic Signed Digit (CSD) arithmetic is used to optimize the multiplier structure of the FIR filter. This filter is implemented based on a semi-custom design flow and a 130nm CMOS standard cell library. This decimation filter operates at 98.304 MHz and provides 32-bit output data at an audio frequency of 192 kHz with power consumption of $697{\mu}W$. In comparison to the previous work, this design shows a higher performance in resolution, operation frequency and decimation factor with lower power consumption and small logic utilization.

Evaluating Reliability of Rooftop Thermal Infrared Image Acquired at Oblique Vantage Point of Super High-rise Building (초고층건물의 사각조망에서 촬영된 지붕표면 열화상의 신뢰도 평가)

  • Ryu, Taek-Hyoung;Um, Jung-Sup
    • Journal of the Korean Solar Energy Society
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    • v.33 no.5
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    • pp.51-59
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    • 2013
  • It is usual to evaluate the performance of the cool roof by measuring in-site rooftop temperature using thermal infra-red camera. The principal advantage of rooftop thermal infrared image acquired in oblique vantage point of super high-rise building as a remote sensor is to provide, in a cost-effective manner, area-wide information required for a scattered rooftop target with different colors, utilizing wide view angle and multi-temporal data coverage. This research idea was formulated by incorporating the concept of traditional remote sensing into rooftop temperature monitoring. Correlations between infrared image of super high-rise building and in-situ data were investigated to compare rooftop surface temperature for a total of four different rooftop locations. The results of the correlations analyses indicate that the rooftop surface temperature by the infrared images of super high-rise building alone could be explained yielding $R^2$ values of 0.951. The visible permanent record of the oblique thermal infra-red image was quite useful in better understanding the nature and extent of rooftop color that occurs in sampling points. This thermal infrared image acquired in oblique vantage point of super high-rise made it possible to identify area wide patterns of rooftop temperature change subject to many different colors, which cannot be acquired by traditional in-site field sampling. The infrared image of super high-rise building breaks down the usual concept of field sampling established as a conventional cool roof performance evaluation technique.

Design of DUC/DDC for the Underwater Basestation Based on Underwater Acoustic Communication (수중기지국 수중 음향 통신을 위한 DUC/DDC 설계)

  • Kim, Sunhee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.5
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    • pp.336-342
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    • 2017
  • Recently, there has been an increasing need for underwater communication systems to monitor ocean environments and prevent marine disasters, as well as to secure ocean resources. Most underwater communication systems adopted acoustic communication with a consideration of attenuation, absorption, and scattering in conductive sea water, and developed fully digital modems based on processors. In this study, a digital up converter (DUC) and a digital down converter (DDC) was developed for an underwater basestation based on underwater acoustic communication systems. Because one of the most important issues in underwater acoustic communication systems is low power consumption due to environmental problems, this study developed a specific hardware module for DUC and DDC. It supported four links of underwater acoustic communication systems and converted the sampling rate and frequency. The systemwas designed and verified using Verilog-HDL in ModelSim environment with the test data generated from baseband layer parts for an underwater base station.

The Influence of Artificial Structures on Benthic Macroinvertebrate Communities in Streams (하천의 인공구조물이 저서성 대형무척추동물 군집에 미치는 영향)

  • Kim, Bong Sung;Sim, Kwang Sub;Kim, Sun Hee;Kwon, O Chang;Seo, Eul Won;Lee, Jong Eun
    • Journal of Environmental Science International
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    • v.22 no.3
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    • pp.309-318
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    • 2013
  • This study was conducted for determining the influence of artificial structures on benthic macroinvertebrate communities in stream. Sampling was taken at upper(pool), down(riffle) and control(riffle) from two check dams, two weirs, one agricultural reservoir, and one multipurpose dam in northern part of Gyeongsangbuk-do. The benthic macroinvertebrate communities of these structures were surveyed during 2009 to 2011. The simple habitat of benthic macroinvertebrates occurred at the upper sites due to pooling effects from artificial structures. Specifically, Check dam1, Jusanji, Imha dam showed very low biological attribute values compared to the down and control sites, which have greater difference in substrate characteristics. However, in the upper sites of Check dam2, Weir1 and Weir2, the difference of values of biological attributes was relatively smaller. Also, proportion of functional feeding groups and functional habit groups were relatively simpler at upper stream and the degree of community differences was greater between upper and down, control sites. Spearman's correlation between biological attributes and substrate characteristics, water quality parameters had significant correlations; particularly, the substrate characteristics were more significantly related. In conclusion, the pool caused by artificial structures had negative effects on benthic macroinvertebrate communities thus leading to simplified stream habitats at upper stream ecosystems.

Research on Digital Complex-Correlator of Synthetic Aperture Radiometer: theory and simulation result

  • Jingye, Yan;Ji, Wu;Yunhua, Zhang;Jiang, Changhong;Tao, Wang;Jianhua, Ren;Jingshan, Jiang
    • Proceedings of the KSRS Conference
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    • 2002.10a
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    • pp.587-592
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    • 2002
  • A new digital correlator fur an airborne synthetic aperture radiometer was designed in order to replace the conventional analog correlator unit which will become very complicated while the number of channels is increasing. The digital correlator uses digital IQ demodulator instead of the intermediate frequency (IF) phase shifter to make the correlation processing performed digitally at base band instead of analogly at IF. This technique has been applied to the digital receiver in softradio. The down-converted IF signals from each pair of receiver channels become low rate base-band digital signals after under-sampled, Digitally Down-Converted (DDC), decimated and filtered by FIR filters. The digital signals are further processed by two digital multipliers (complex correlation), the products are integrated by the integrators and finally the outputs from the integrators compose of the real part and the imaginary part of a sample of the visibility function. This design is tested by comparing the results from digital correlators and that from analog correlators. They are agreed with each other very well. Due to the fact that the digital correlators are realized with the help of Analog-Digital Converter (ADC) chips and the FPGA technology, the realized volume, mass, power consumption and complexity turned out to be greatly reduced compared with that of the analog correlators. Simulations show that the resolution of ADC has an influence on the synthesized antenna patterns, but this can be neglected if more than 2bit is used.

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Screening for down syndrome using trophoblast retrieval and isolation of the cervix: preliminary study

  • Lee, Min Jin;Kim, Soo Hyun;Park, Hee Jin;Shim, Sung Han;Jang, Hee Yeon;Cha, Dong Hyun
    • Journal of Genetic Medicine
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    • v.17 no.2
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    • pp.68-72
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    • 2020
  • Purpose: Trisomy 21, the cause of Down syndrome (DS) with various medical problems, is the most common aneuploidy during the fetal period. For diagnosis, a non-invasive screening test using maternal blood, which cannot be confirmed and invasive confirmation test with a risk of miscarriage, may be performed. The trophoblast retrieval and isolation of the cervix (TRIC) have been proposed by some researchers as an alternative to overcome the limitations of current tests. We experimented using TRIC to identify the possibility of trisomy 21 for the first time in Asia. Materials and Methods: Three cases of DS were analyzed confirmed by invasive tests (chorionic villus sampling, amniocentesis). All samples of trophoblasts immediately were immersed in phosphate-buffered saline and processed with formalin for fixation. The trophoblasts were isolated using an anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. β-human chorionic gonadotropin (hCG)-expressing cells were considered as trophoblast cells, and the detection rate calculated. DS was confirmed by fluorescence in situ hybridization (FISH). Results: The mean trophoblast detection rate using β-hCG was 78.1%, and the detection rate using FISH was 22.2%. In all cases, the trisomy of chromosome 21 was identified. Conclusion: Trophoblast can be obtained from the five weeks of gestation and has a high detection rate, so it is noted that it can replace the current prenatal genetic test. To realize the clinical application as a prenatal genetic test, we will need additional efforts to identify trisomy 21 as well as other chromosomal abnormalities in future large-scale studies.

Design of Receiver in High-Speed digital Modem for High Resolution MRI (고속 디지털 MRI 모뎀 수신기 설계)

  • 염승기;양문환;김대진;정관진;김용권;권영철;최윤기
    • Proceedings of the IEEK Conference
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    • 2000.06a
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    • pp.69-72
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    • 2000
  • This paper shows the more improved design of MRI receiver compared to conventional one based on Elscint Spectrometer. At first, the low-cost ADC is 16 bits, 3MHz sampling A/D converter Comparing to conventional one with signal bits of 14 bits, this device with those of 16 bits helps getting Improved the image resolution improved. If frequency is designed centering around 7.6 MHz to be satisfied in 10 MHz of maximum input bandwidth of ADC. For 1st demodulation, fixed IF is used for the purpose of the implementing multi nuclei system. Control parts & partial digital parts are integrated on one chip(FPGA). In DDC(Digital Down Converter), we got required bandwidth of LPF by controlling its decimation rate. With above considerations, we designed optimal receiver for high resolution imaging to be implemented through PC interface & experimental test of receiver of MRI after receiver's fabrication.

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