• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2003.10a
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• pp.41-41
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• 2003

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimps in Korea and many other countries. Since 1993, massive mortalities due to the viral infection have also occurred in the penaeid shrimps cultured in Korea. WSSV is a large, circular, double stranded (ds) DNA virus and an enveloped, ellipsoid virus with a rod-shaped nucleocapsid with flat ends. In order to identify the characteristics of this Korean isolate of WSSV, the genes for four virion proteins, VP15, VP19, VP28 and VP35 were cloned and their sequences were compared with the available pool of WSSV gene sequences in the GenBank/EMBL databases. From these comparisons, we confirm the occurrence of WSSV in Korea and deduce that, VP15, VP28 and VP35 genes are identically conserved among the Korean isolate and geographically different foreign isolates, but VP19 amino acid sequences of the Korean WSSV isolates changed valine of the foreign isolates into aspartate. (omitted)

• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.219-224
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• 2002

We report the seasonal prevalence of the mycoplasmal pneumoniae of swine (MPS) in slaughter pigs from July of 1999 to June of 2000. Gross finding of lung lesion observed and examined by dot-ELISA. In gross finding of lung lesion from 750 pig samples, 465 (62.0%) was MPS, and 129 (17.2%) was single or double infection with actinobacillosis and pasturellosis. However, 156 (20.8%) had no lesion. In seasonal detection, the prevalence was found to be winter (69.5%), autumn (63.5%), summer (60.0%) and spring (54.7%) in orderly frequency. In dot-ELISA, the result was showed the positive reaction (x16>titre) with 58.0% and negative (x4

• Korean Journal of Veterinary Service
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• v.21 no.4
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• pp.391-400
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• 1998

In order to survey the prevalence of intestinal parasites in dogs, 304 fecal samples were taken from dogs in Taejon city, The prevalence and identification of intestinal parasites were determined by the fecal examinations using sheather's floating technique and sedimentation methods and then Cryptosporidium oocysts were identified by kinyoun's modified acid fast stainning method. The results were obtained as follows ; 1. Parasite eggs and oocysts were detected in 105 samples (34.5%) from 304 cases of dog feces. 2. The 11 kinds of eggs and oocysts were isolated from the feces of dog. Those were Ancylostoma caninum (12.1%, 37 dogs), Trichuris vulpis (11.5%, 35 dogs), Toxocara canis (10.2%, 31 dogs), Isospora sp (7.2%, 22 dogs), Cryptosporidium sp (3.6%, 11 dogs), Toxascaris leonine (1.9%, 6 dogs), Strongyloides sp (1.9%, 6 dogs), Taenia sp (0.6%, 2 dogs), Diphylidium caninum (0.3%, 1 dog), Spirometra sp (0.3%, 1 dog) and Clonorchis sinensis (0.3%, 1 dog). 3. It was mixed infection such as single, double, triple and quadruple, 64.8%, 25.7%, 8.6% and 0.9%, respectively. 4. In indiviually-raised 4095, the infectious late of T canis (11.4%), A Caninum(13.2%), Cryptosporidium sp (6.1%), T leontna (2.6%) were higher than those of group raised dogs. But the infectious rate of T vulpis (12.1%) in group raised dogs was higher than that of individually-raised dogs. 5. Adults of Demodex and Sarcoptes which have been found in this survey are excluded in this report.

• Journal of Microbiology
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• v.34 no.1
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• pp.7-14
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• 1996

Teh baculovirus gp64 glycoprotein is a major component of the envelope of budded virus (BV) and has been shown that it plays an essential role in the infection process, especially virus-cell membrane fusion. We have cloned Autographa californica Nuclear Polyhedrosis Virus (AcNPV) gp64 protein were examined for membrane fusion activity by using a synchtium formation assay under various conditions. The optimal conditions required for inducing membrane fusion are 1) form pH 4.0 to 4.8 2) 15 min exposure of cells to acidic pH 3) at least 1 .mu.g of gp64 cloned plasmid DNA per 3 * 10$^{6}$ cells 4) and an exposure of cells to acidic pH at 72 h post-transfection. In order to investigate the role of hydrophobicity of the gp64 glycoprotein for the membrane fusion, the two leucine residues (amino acid position at 229 and 230) within hydrophobic region I were substituted to alanine by PCR-derived site-directed mutagenisis and the membrane fusion activity of the mutant was anlaysed. The gp64 glycoprotein carrying double alamine substitution mutation showed no significant difference in fusion activity. This result suggested that minor changes in hydrophobicity at the amino acid position 229 and 230 does not affect the acid-induced membrane fusion activity of the gp64 glycoprotein.

• Applied Microscopy
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• v.5 no.1
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• pp.31-43
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• 1975

These studies were carried out to detect the presence of infected virus- like particles and also were observed the ultrastructures of Aspergillus ochraseus isolated from kokja and Korean ginseng. The results of ultrastructures of Aspergillus ochraseus are summarized as follows: 1. In fungal cells, nuclei were enclosed by a irregular double membrane and nucleoli in the nucleus. 2. In cytoplasm, mitochondria, rough endoplasmic reticulum with ribosomes and glycogen were scattering distributed and many lomasomes also observed. 3. The osmiophilic bodies of fungal cells existed in the vesicles. 4. The cell walls were composed of a low electron dense materials. 5, Conidia cell walls were extremely thick and possessed the high electron density of outer coat. The virus-like particles were observed in the hyphae of Penicillium chrysogenum Q-176. These virus-like particles measured $350{\AA}$ in diameter. But strains of Aspergillus ochraseus, showing some vesicle particles were also observed about $800{\AA}$ in diameter in the central region of young fungal hyphae. Based on the results of these experiments, it can not be determined virus particles or not. The further studies to determination of virus particles will be proceeded by the chemical, physical and biological assay methods.

• Journal of Chest Surgery
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• v.16 no.3
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• pp.289-294
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• 1983

The report is concerned to our experience of 12 cases of open heart surgery under the extracorporeal circulation at the Department of Thoracic and Cardiovascular Surgery, Chosun University Hospital during the period between Nov, 1979 and April, 1983. 1. There were 4 cases of congenital anomaly and 8 cases of acquired heart disease. 2. There were 6 male and 6 female patients with a mean age of 20 years. [range 9 to 33 years]. 3. The cases induced 2 ventricular septal defect, 2 atrial septal defects and 8 acquired valvular heart diseases. 4. The surgical managements were 2 primary repair for atrial septal defect and 2 patch closure for ventricular septal defect, 1 triple valve replacement [AVR MVR TVR], 1 aortic valve replacement, 4 double valve replacement [AVR MVR] and 2 open mitral commissurotomy for pure mitral stenosis. 5. The average cardiopulmonary bypass time was 61.5 minutes for congenital heart disease and 201.4 minutes for acquired valvular heart disease and the average aortic cross clamping time was 36.75 minutes for the former and 165.6 minutes for the latter. 6. Postoperatively, there were 1 Alopecia, 1 Electric burn and 1 wound infection as complication. 7. Overall operative mortality was 8.3%. 7. All patients received valve replacement were recommended anticoagulation with persantin.

• Korean Journal of Veterinary Service
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• v.28 no.2
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• pp.107-112
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• 2005

In order to monitor the parasites, 204 fecal samples were taken from Korean indigenous goats of Iksan-branch. Then identification of the parasites was determined by the fecal examination using the floatation and microscopical examination, respectively. The detection of rates was $91.2\%$, and mixed infection rates were single $38.7\%,\;double\;28.4\%,\;triple\;15.2\%,\;Quadraple\;6.9\%\;and\;Qunituple\;20.0\%$. The isolated were identified as Eimeria spp from 169 heads, Strongyloides papillosus from 56 heads, Ostertagia spp from 24 heads, Trichostrongylus spp from 22 heads, Moniezia expensa from 18 heads, Oesophagostomum spp from 17 heads, Bonostomum spp from 12 heads, Cooperia spp from 12 heads, Heamonchus spp from 8 heads and Capillaria spp from 2 heads.

• Molecules and Cells
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• v.21 no.1
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• pp.21-28
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• 2006

The interferon-induced, double-stranded RNA (dsRNA)-dependent protein kinase PKR plays a key role in interferon-mediated host defense against viral infection, and is implicated in cellular transformation and apoptosis. We have isolated a cDNA of simian PKR encoding a product with 83% amino acid identity to the human homolog and showed that PKR expression is significantly attenuated in the Vero E6 African green monkey kidney cells devoid of type I interferon genes. A variant form of PKR lacking the exon 12 in the kinase domain is produced in these cells, presumably from an alternatively spliced transcript. Unlike wild type PKR, the variant protein named PKR-${\Delta}E12$ is incapable of auto-phosphorylation and phosphorylation of eIF2-${\alpha}$, indicating that the kinase sub-domains III and IV embedded in exon 12 are indispensable for catalytic function. PKR-${\Delta}E12$ had no dominant negative effect but was weakly phosphorylated in trans by wild type PKR.

• Korean Journal of Clinical Laboratory Science
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• v.43 no.1
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• pp.26-31
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• 2011

The genotypes of Human Papilloma Virus (HPV) are important in the carcinogenesis of uterine cervical cancer. Diagnosis of uterine cervical cancer screening has been executed using Papanicolau method (Pap) and HPV DNA Chip method. We researched the interrelation of HPV DNA genotypes in single and multiple infections and analyzed the results of Pap and HPV DNA Chip tests at Gunsan Medical Center (GMC). The correlation analysis was surveyed on collected results from 599 patients who have been tested with both Pap and HPV DNA chip tests from November 2004 to May 2010 at GMC. The inconsistency between Pap and HPV DNA Chip tests was 41.1%. The HPV DNA Chip genotype related with high risk cases were type 16 (13.5%), type 52 (10.5%), type 58 (10.1%), and type 18 (3.4%). Those related with low risk cases were type 70 (8.9%), type 6 (1.7%), type 40 (1.2%), type 11 (1.3%), and other types (14.3%). Among the 195 cases of HPV positive status, 161 cases were associated with single infection; 108 (67.1%) cases were related with high risk genotype; 19 (11.8%) cases were low risk genotype; 31 (21.1%) cases were related with other types. 29 cases were associated with double infections; 23 (79.3%) cases were high risks; 5 (17.2%) cases were mixed high and low risks; 1 (3.5%) case was low risk.

• Journal of Korean Society of Forest Science
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• v.6 no.1
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• pp.6-10
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• 1967

In the present paper author investigated the symptom, pathogenic fungus and pathogenicity of Endothia canker of Carpinus laxiflora in Korea, and made clear the indistinct discription on its pathogen in the past. 1. The pathogen is identified as Endothia fluens (Schw.) Shear et Stevens. The discription is recorded as follows: Stromata cortical, erumpent, spherical or conical, outer yellowish-brown and inner yellowish, 0.5 to 2.5 mm in diameter; perithecia irregularly embeded in the bottom of stroma, 7 to 23 in a stroma usually spherical to elliptical or irregular, 235 to $370{\mu}$ in diameter, with black slender necks; each neck open the papilliate ostiole to the surface, about 250 to $400{\mu}$ in length; asci clavate or fusoid, colorless, 31.16 to 42.64 by 6.54 to $8.20{\mu}$ in size, average 37.02 by $6.84{\mu}$, with 8 ascospores in double line; ascospores elliptical, ovate or cylindrical, with rounded ends, hyaline, 1-septate, not constrict at the septum, 6.51 to 9.30 by 3.16 to $3.72{\mu}$, average 7.61 by $3.44{\mu}$ in size; pycnidia formed abundantly in stroma. spherical at first but later irregular large cavity by fussing each other; pycnospores oblong or rod-shaped, hyaline, non-septate, 3.8 by $1.9{\mu}$ in size; spore-horn formed abundantly under moist condition. 2. The pathogen is wound parasite invading the hosts through the wound. But after the infection is established, expanding the disease lesion is swiftly vigorus.