• 한국어류학회지
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• 제33권2호
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• pp.148-154
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• 2021

매가오리목 매가오리과에 속하는 Mobula thurstoni 2개체(1770~1850 mm 체반폭)가 2018년 9월 전라남도 여수시 연도 연안에서 정치망으로 채집되었다. 이 종은 가슴지느러미의 앞부분이 이중 굴곡이고, 등지느러미 바로 뒤 꼬리 시작부분에 가시가 없으며, 등지느러미 끝부분에는 흰색이고, 그리고 등쪽의 체색이 어두운 남색을 띤다. Mobula kuhlii와 가장 형태적으로 유사하였지만, 가슴지느러미 앞부분에 이중 굴곡을 가지고 있다는 점(vs. 직선이거나 약간의 굴곡을 가진다)과 등쪽 체색이 어두운 남색을 띤다는 점(vs. 회갈색)에서 잘 구분된다. 또한, 이 종은 M. kuhlii와 미토콘드리아 16S rRNA 영역에서 유전적 거리 0.030~0.069의 차이를 보여 구분되었다. 이 종의 새로운 국명으로 '매끈꼬리쥐가오리'를 제안한다.

• BMB Reports
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• 제51권11호
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• pp.578-583
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• 2018

Telomeres are specialized nucleoprotein complexes that function to protect eukaryotic chromosomes from recombination and erosion. Several telomere binding proteins (TBPs) have been characterized in higher plants, but their detailed in vivo functions at the plant level are largely unknown. In this study, we identified and characterized OsTRFL1 (Oryza sativa Telomere Repeat-binding Factor Like 1) in rice, a monocot model crop. Although OsTRFL1 did not directly bind to telomere repeats $(TTTAGGG){_4}$ in vitro, it was associated with telomeric sequences in planta. OsTRFL1 interacted with rice TBPs, such as OsTRBF1 and RTBP1, in yeast and plant cells as well as in vitro. Thus, it seems likely that the association of OsTRFL1 with other TBPs enables OsTRFL1 to bind to telomeres indirectly. T-DNA inserted OsTRFL1 knock-out mutant rice plants displayed significantly longer telomeres (6-25 kb) than those (5-12 kb) in wild-type plants, indicating that OsTRFL1 is a negative factor for telomere lengthening. The reduced levels of OsTRFL1 caused serious developmental defects in both vegetative and reproductive organs of rice plants. These results suggest that OsTRFL1 is an essential factor for the proper maintenance of telomeres and normal development of rice.

• Parasites, Hosts and Diseases
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• 제45권1호
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• pp.1-9
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• 2007

The endosymbionts of 4 strains of Acanthamoeba(KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of Acanthamoeba isolates. The endosymbionts of KA/E9, KA/E22, and KA/E23 were surrounded by electron-translucent areas. No lacunae-like structures were observed in the endosymbionts of KA/E21, the bacterial cell walls of which were studded with host ribosomes. Comparative analyses of the 16S rDNA sequences showed that the endosymbionts of KA/E9, KA/E22 and KA/E23 were closely related to Caedibacter caryophilus, whereas the KA/E21 endosymbiont was assigned to the Cytophaga-Flavobacterium-Bacteroides(CFB) phylum. In the 4 strains of Acanthamoeba, the hosts of the endosymbionts were identified as belonging to the Acanthamoeba castellanii complex, which corresponds to the T4 genotype. Acanthamoeba KA/E21 evidenced characteristics almost identical to those of KA/E6, with the exception of the existence of endosymbionts. The discovery of these endosymbionts from Acanthamoeba may prove essential to future studies focusing on interactions between the endosymbionts and the amoebic hosts.

• The Plant Pathology Journal
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• 제27권3호
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• pp.285-290
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• 2011

Fusarium graminearum virus 2 (FgV2) infects Fusarium graminearum strain 98-8-60 and has at least five segments of double-stranded RNAs (dsRNAs), denoted as dsRNA-1 to dsRNA-5. In this study, the genome of FgV2 was sequenced and its phylogenetic relationship with other mycoviruses was analyzed. The lengths of FgV2 dsRNAs 1-5 ranged from 2414 to 3580 base pairs (bp). The 5' and 3' untranslated regions (UTRs) are highly conserved, and each dsRNA segment had 78-105 and 84-306 bp of 5' and 3' UTRs, respectively. Each dsRNA segment contained a single open reading frame (ORF). Computer analysis of dsRNA-1 revealed a putative open reading frame (ORF) that shows high sequence identity with an RNA-dependent RNA polymerase (RdRp) containing eight conserved motifs. dsRNAs 2-5 also each contain one putative ORF coding for products of unknown function. The sequences of FgV2 dsRNA-2 and dsRNA-3 have significant sequence identity with Magnaporthe oryzae chrysovirus 1 (MoCV1) dsRNA-3 and -4, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis of the putative RdRp protein, FgV2 was found to form a distinct virus clade with Aspergillus mycovirus 1816 and MoCV1 in the family Chrysoviridae.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3223-3228
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• 2011

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, $Ru(phen)_2(dppz)^{2+}$ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)${\cdot}$poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of $Hg^{2+}$ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as $K^+$, $Ag^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Fe^{3+}$, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.

• The Plant Pathology Journal
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• 제34권5호
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• pp.426-434
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• 2018

During the spring season of 2014, a total of 148 melon and watermelon leaf samples were collected from symptomatic and asymptomatic plants in the western and southwestern regions of Saudi Arabia and were tested for the presence of Watermelon chlorotic stunt virus (WmCSV) and other suspected cucurbit viruses by double antibody sandwich enzyme-linked immunosorbent assays. Ninety-eight samples were found to be positive for the presence of WmCSV, nine samples were positive for the presence of Cucurbit yellow stunting disorder virus (CYSDV), and 22 showed a mixed infection with both WmCSV and CYSDV. No other cucurbit viruses were detected in any of the samples. Host range experiments revealed that eight out of fourteen tested plant species were susceptible to WmCSV. PCR products of approximately 1.2 kb were obtained after amplification using primers specifically targeting the coat protein region of WmCSV. Positive PCR results were confirmed by dot blot hybridization. Coat protein gene sequences from eleven WmCSV isolates indicated that the highest identity was between the 104WMA-SA isolate from the Wadi Baish location and a previously reported isolate from the AL-Lith location in Saudi Arabia. The lowest identity was observed between the 42WMA-SA isolate and an isolate from Palestine.

• Asian-Australasian Journal of Animal Sciences
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• 제27권3호
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• pp.324-329
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• 2014

To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to create targeted mutations and enriched using a reporter plasmid system. The reporter expresses RFP and eGFP simultaneously when ZFN-mediated site-specific mutations occur. Thus, double positive cells ($RFP^+/eGFP^+$) were selected and used for somatic cell nuclear transfer. Two types of reporter based enrichment systems were used in this study; the cloned embryos derived from cells enriched using a magnetic sorting-based system showed better developmental competence than did those derived from cells enriched by flow cytometry. Mutated sequences, such as insertions, deletions, or substitutions, together with the wild-type sequence, were found in the cloned porcine blastocysts. Therefore, genetic mutations can be achieved in cloned porcine embryos reconstructed with ZFN-treated cells that were enriched by a reporter-based system.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 추계종합학술대회 B
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• pp.285-288
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• 2008

기계 학습을 응용한 많은 침입 탐지 시스템들은 n-그램 접근 방법을 주로 쓰고 있다. 그러나, n-그램 접근 방법은 주어진 시퀀스에서 획득한 n-그램들이 서로 겹치는 문제들을 가지고 있다. 본 연구에서는 이러한 문제들을 해결하기 위해, n-그램 증강 나이브 베이스 (n-gram augmented naive Bayes) 알고리즘을 침입 시퀀스의 분류에 적용하였다. 제안된 시스템의 성능을 평가하기 위해 n-그램 특징들을 사용하는 일반 나이브 베이스 (naive Bayes) 알고리즘과 서포트 벡터 머신 (support vector machines) 알고리즘과 본 연구에서 제안한 n-그램 증강 나이브 베이스 알고리즘을 비교하였다. 뉴 멕시코 대학의 벤치마크 데이터에 적용해 본 결과에 따르면, n-그램 증강 방법이, n-그램이 나이브 베이스에 직접 적용되는 경우(예: n-그램 특징을 사용하는 일반 나이브 베이스), 생기는 독립성 가정에 대한 위배 문제도 해결하면서, 동시에 n-그램 특징을 사용하는 일반 나이브 베이스보다 더 정확하며, n-그램 특징을 사용하는 SVM과 필적할만한 수준의 침입 탐지기를 생성해 내었다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 추계학술대회
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• pp.103-106
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• 2012

실시간 영상신호압축에서 일정 분량의 신호를 저장한 후 다른 순서로 읽어내는 과정은 간단하지만 JPEG, MPEG1/2/4, H.264, HEVC 등의 거의 모든 표준에서 필수적으로 사용하고 있는 중요한 과정이다. 실시간 처리가 중요하기 때문에 지금까지는 필요한 영상 블럭 크기의 메모리를 두 개 이용하여 동시에 번갈아 가며 읽고 쓰는 이중 버퍼링 방법을 사용하였다. 예외적으로 2D DCT에서의 전치버퍼의 경우는 입출력 순서가 단순하기 때문에 단일 버퍼링을 이용하여 입출력 순서의 변환이 가능하다. 본 논문에서는 불규칙한 임의의 입출력 순서에서도 유한한 횟수 안에 규칙적 형태의 입출력 순서열이 반복됨을 보이고, 그것을 이용하여 단일 메모리를 사용하는 효율적인 실시간 메모리 입출력 기법을 구현하였다.

• 방송공학회논문지
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• 제22권3호
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• pp.381-396
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• 2017

본 논문에서는 주관적 화질 기반의 비트 분배를 수행하는 율 제어 알고리즘을 수행하는 HEVC (High Efficiency Video Coding) 부호화 방법을 위한 연구를 진행하였다. 본 논문은 이러한 단점을 해소하고자 율 왜곡 최적화 시의 화질 측정에서 주관적 화질을 고려할 수 있는 율 제어 알고리즘을 통한 HEVC 부호화 방법을 제안한다. 제안하는 방법은 영상을 하나의 CTU 마다 인지 시각적 중요도를 측정하여, 이를 이용하여 픽쳐 단위, CTU 단위에의 비트 분배 시 적응적인 분배를 수행한다. 본 논문에서 제안하는 방법은 HEVC 참조 소프트웨어 16.9 버전 대비 CTC (Common Test Condition) Class B 영상에서 평균적으로 BD-rate 3.12%의 성능향상과 BD-PSNR의 0.08dB 향상 및 목표 비트율에의 비트 정확도 0.07% 증가를 보였다. 또한 주관적 화질 측정 결과도 기존 HEVC의 참조 소프트웨어에 적용된 율 제어 알고리즘 대비 DSCQS 스케일에서 평균 0.16 향상된 것을 확인하였다.