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http://dx.doi.org/10.5423/PPJ.OA.04.2018.0059

Insights into the Incidence of Watermelon chlorotic stunt virus Causing Yellowing Disease of Watermelon in Western and Southwestern Regions of Saudi Arabia  

Ahmad, M.H. (Plant Protection Department, College of Food and Agriculture Sciences, King Saud University)
Shakeel, M.T. (Department of Plant Pathology, Bahauddin Zakariya University)
Al-Shahwan, I.M. (Plant Protection Department, College of Food and Agriculture Sciences, King Saud University)
Al-Saleh, M.A. (Plant Protection Department, College of Food and Agriculture Sciences, King Saud University)
Amer, M.A. (Plant Protection Department, College of Food and Agriculture Sciences, King Saud University)
Publication Information
The Plant Pathology Journal / v.34, no.5, 2018 , pp. 426-434 More about this Journal
Abstract
During the spring season of 2014, a total of 148 melon and watermelon leaf samples were collected from symptomatic and asymptomatic plants in the western and southwestern regions of Saudi Arabia and were tested for the presence of Watermelon chlorotic stunt virus (WmCSV) and other suspected cucurbit viruses by double antibody sandwich enzyme-linked immunosorbent assays. Ninety-eight samples were found to be positive for the presence of WmCSV, nine samples were positive for the presence of Cucurbit yellow stunting disorder virus (CYSDV), and 22 showed a mixed infection with both WmCSV and CYSDV. No other cucurbit viruses were detected in any of the samples. Host range experiments revealed that eight out of fourteen tested plant species were susceptible to WmCSV. PCR products of approximately 1.2 kb were obtained after amplification using primers specifically targeting the coat protein region of WmCSV. Positive PCR results were confirmed by dot blot hybridization. Coat protein gene sequences from eleven WmCSV isolates indicated that the highest identity was between the 104WMA-SA isolate from the Wadi Baish location and a previously reported isolate from the AL-Lith location in Saudi Arabia. The lowest identity was observed between the 42WMA-SA isolate and an isolate from Palestine.
Keywords
Coat protein; ELISA; PCR; WmCSV; yellowing;
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1 Jones, P., Sattar, M. H. A. and Al Kaff, N.1988. The incidence of virus disease in watermelon and sweet melon crops in the People's Democratic Republic of Yemen and its impact on cropping policy. Ann. Appl. Biol. 17:203-207.
2 Khan, A. J., Akhtar, S., Briddon, R. W., Ammara, U., Al-Matrooshi, A. M. and Mansoor, S. 2012. Complete nucleotide sequence of watermelon chlorotic stunt virus originating from Oman. Viruses 4:1169-1181.   DOI
3 Kheyr-Pour, A., Bananej, K., Dafalla, G. A., Caciagli, P., Noris, E., Ahoonmanesh, A., Lecoq, H. and Gronenborn, B. 2000. Watermelon chlorotic stunt virus from the Sudan and Iran: sequence comparisons and identification of a whitefly-transmissible determinant. Phytopathology 90:629-635.   DOI
4 Nelson, S. C. and Bushe, B. C. 2006. Collecting plant disease and insect pest samples for problem diagnosis. University of Hawaii, Manoa, Honolulu, Hawaii, USA.
5 Pallas, V., Mas, P. and Sanches-Navarro, J. A. 1998. Detection of plant RNA viruses by non-isotopic dot-blot hybridization. In: Plant Virology Protocols, ed. by G. D. Foster, S. C. Taylor, pp. 461-468. Springer, New York, USA.
6 Podleckis, E. V., Hammond, R. W., Hurtt, S. S. and Hadidi, A. 1993. Chemiluminescent detection of potato and pome fruit viroids by digoxigenin-labeled dot blot and tissue blot hybridization. J. Virol. Methods 43:147-158.   DOI
7 Robinson, R. W. and Decker-Walters, D. S. 1997. Cucurbits. CAB International, Wallingford, UK. 226 pp.
8 Sambrook, J. and Russell, D. W. 2001. Molecular Cloning: A Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory, New York, NY, USA. 999 pp.
9 Sanchez-Navarro, J. A., Aparicio, F., Rowhani, A. and Palla's, V. 1998. Comparative analysis of ELISA, nonradioactive molecular hybridization and PCR for the detection of prunus necrotic ringspot virus in herbaceous and Prunus hosts. Plant Pathol. 47:780-786.   DOI
10 Wang, Y. H., Thomas, C. E. and Dean, R. A. 1997. A genetic map of melon (Cucumis melo L.) based on amplified fragment length polymorphism (AFLP) markers. Theor. Appl. Genet. 95:791-798.   DOI
11 Wiley, J. and Sons, 2012. In: Crop Post-Harvest: Science and Technology, Volume 3: Perishables. ed by Debbie Rees, Graham Farrell, John Orchard (Eds), In.: Steven, A. S., Donald, N. M. (Eds.) Cucurbits. pp. 286-316. Wiley-Blackwell, Chichester.
12 Zerbini, F. M., Briddon, R. W., Idris, A., Martin, D. P., Moriones, E., Navas-Castillo, J., Rivera-Bustamante, R., Roumagnac, P., Varsani, A. and Consortium, I. R. 2017. ICTV Virus Taxonomy Profile: geminiviridae. J. Gen. Virol. 98:131-133.   DOI
13 Zitter, T. A., Hopkins, D. L. and Thomas, C. E. 1996. Compendium of Cucurbit diseases. American Phytopathological Society, St. Paul, MN, USA. 87 pp.
14 Walkey, D. G. A., Alhubaishi, A. A. and Webb, M. J. W. 1990. Plant virus disease in the Yemen Arab Republic. Trop. Pest Manage. 36:195-206.   DOI
15 Abou-Jawdah, Y., Sobh, H., Haidar, A. and Samsatly, J. 2010. First report in Lebanon on detection of two whitefly transmitted cucurbit viruses and their molecular characterization. MPU, Rome, Italy.
16 Abudy, A., Sufrin-Ringwald, T., Dayan-Glick, C., Guenoune-Gelbart, D., Livneh, O., Zaccai, M. and Lapidot, M. 2010. Watermelon chlorotic stunt and Squash leaf curl begomoviruses-New threats to cucurbit crops in the Middle East. Isr. J. Plant Sci. 58:33-42.   DOI
17 Ali-Shtayeh, M. S., Jamous, R. M., Mallah, O. B. and Abu-Zeitoun, S. Y. 2014. Molecular characterization of watermelon chlorotic stunt virus (WmCSV) from Palestine. Viruses 6:2444-2462.   DOI
18 Bananej, K., Ahoonmanesh, A. and Kheyr-Pour, A. 2002. Host range of an Iranian isolate of watermelon chlorotic stunt virus as determined by whitefly-mediated inoculation and agroinfection, and its geographical distribution. J. Phytopathol. 150:423-430.   DOI
19 Al-Musa, A., Anfoka, G., Al-Abdulat, A., Misbeh, S., Haj Ahmed, F. and Otri, I. 2011. Watermelon chlorotic stunt virus (WmCSV): a serious disease threatening watermelon production in Jordan. Virus Genes 43:79-89.   DOI
20 Al-Saleh, M. A., Ahmad, M. H., Al-Shahwan, I. M., Brown, J. K. and Idris, A. M. 2014. First report of watermelon chlorotic stunt virus infecting watermelon in Saudi Arabia. Plant Dis. 98:1451-1451.
21 Bedford, I. D., Briddon, R. W., Brown, J. K., Rosell, R. C. and Markham, P. G. 1994a. Gemini viruses transmission and biological characterization of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Ann. Appl. Biol. 125:311-325.   DOI
22 Bedford, I. D., Briddon, R. W., Jones, P., Alkaff, N. and Markham, P. G. 1994b. Differentiation of the three whitefly-transmitted Gemini viruses from the Republic of Yemen. Eur. J. Plant Pathol. 100:243-257.   DOI
23 Clark, M. F. and Adams, A. M. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483.   DOI
24 Dellaporta, S. L., Wood, J. and Hicks, J. B. 1983. Maize DNA miniprep. Plant Mol. Biol. Rep. 1:19-21.   DOI
25 Erickson, D. L., Smith, B. D., Clarke, A. C., Sandweiss, D. H. and Tuross, N. 2005. An Asian origin for a 10,000-year-old domesticated plant in the Americas. Proc. Natl. Acad. Sci. U.S.A. 102:18315-18320.   DOI