Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Emission Detection of Mercuric Ions in Aqueous Media Based-on Dehybridization of DNA Duplexes

  • Oh, Byul-Nim (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Wu, Qiong (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Cha, Mi-Sun (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Kang, Hee-Kyung (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Kim, Jin-Ah (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Kim, Ka-Young (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Rajkumar, Eswaran (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Kim, Jin-Heung (Department of Chemistry and Nano Science, Ewha Womans University)
  • Received : 2011.06.08
  • Accepted : 2011.07.12
  • Published : 2011.09.20
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Abstract

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, $Ru(phen)_2(dppz)^{2+}$ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)${\cdot}$poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of $Hg^{2+}$ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as $K^+$, $Ag^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Fe^{3+}$, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.

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References

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