• Title/Summary/Keyword: Double Carrier

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Purification and Characterization of Myristoyl-Acyl Carrier Protein Thioesterase from Iris tectorum

  • Kang, Han-Chul;Cho, Kang-Jin;Hwang, Young-Soo
    • Applied Biological Chemistry
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    • v.41 no.4
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    • pp.235-240
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    • 1998
  • The myristoyl-acyl carrier protein (ACP) specific thioesterase from Iris tectorum was purified to a considerable homogeneity and characterized. The enzyme was eluted with a considerable stability by double-gradients using Triton X-100 and low ionic KCl or Na-phosphate through DEAE-52, Octyl-Sepharose, Q-Sepharose, and hydroxyapatite chromatoraphy. SDS-PAGE analysis showed a single band of 39 kDa. The native molecular weight was estimated to be 82 kDa by Sephacryl S-200 chromatography, indicating that the enzyme was a dimer. The thioesterase showed a chain-length specificity to myristoyl-ACP in preference to other-ACPs. The enzyme activity decreased by 1.0 mM myristate to about 27% of the original activity, whereas the remaining activity with decanoate was about 90%. The purified thioesterase was inhibited by myristoyl-CoA more than by myristate, suggesting that the myristoyl-AGP thiolesterase might be controlled by myristic acid and/or a subsequent product myristoyl-CoA. In addition, some biochemical characteristics of the enzyme were described.

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Power Flow Analysis for Manufacturing of Planetary Gears in a 8-Speed Automatic Transmission (I): 1-3 Speeds (8단 자동변속기의 유성기어 가공을 위한 동력 흐름 해석 (I) : 1-3단)

  • Lee, Kyoung-Jin;Kim, Jeong-Min
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.15 no.5
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    • pp.48-56
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    • 2016
  • In this paper, we analyze the power flow of an eight-speed automatic transmission by using a lever analogy for the manufacturing of planetary gears. The results indicate that the engine power is passed down to the carrier and ring gear in the first double pinion planetary gear (DPPG1), and to the sun gear, carrier, and ring gear in DPPG3 for the first speed. Although the power flow is similar in the second speed, the power circulation occurs in the second single pinion planetary gear (SPPG2). For the third speed, the engine power is passed from the carrier to the ring gear in DPPG, at which point the power is split between the sun gears of SPPG2 and DPPG3.

Research of Elements Giving Effects on the Numerical Analysis for the LNG Carrier (LNG 운반선에 대한 수치해 변화에 영향을 주는 요소에 관한 연구)

  • Kim, Hee-Taek;Kim, Hyoung-Tae
    • Journal of the Society of Naval Architects of Korea
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    • v.43 no.6 s.150
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    • pp.656-666
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    • 2006
  • Numerical analysis using the FLUENT is performed for the 138K LNG carrier of the MOERI. In this study, results according to the number and also the type of the grid, different turbulent models, variations of y+ are compared with experiments and computational results from the WAVIS. RSM model gives good result in comparison with other models, and the more the number of grid increase, the better the resistance converges to the constant value. Additionally, it is confirmed that the resistance and wake distributions vary with the change of y+, and O-O type grid yields better wake distributions than that of O-H type. Average velocity distributions and velocity profiles are mainly in accord with experiments and computational results of the WAVIS, however, present results in the region of transom and tip of leading edge of the rudder are poor for the lower velocity components comparing with that of the WAVIS.

Heat Flux Calculation for Thermal Equilibrium of Cofferdam in a LNG Carrier (LNG선 Heating Coil의 설계를 위한 Cofferdam내 열정산)

  • Joo-Ho Heo;Young-Bum Lee
    • Journal of the Society of Naval Architects of Korea
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    • v.35 no.1
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    • pp.98-106
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    • 1998
  • This paper shows the temperature distribution of double hull compartment and of cofferdam in a large LNG Carrier. In LNG Carrier, due to the lower cargo temperature($-163^{\circ}C$), structures are forced to lose their strength if additional heat is not supplied. So it is very important to estimate the temperature distribution and the heat flux needed to maintain the structure properly. The temperature of each compartment is obtained using 2-dimensional model analysis and compared with 3-dimensional results. And also this paper gives preliminary estimation of pipe length to supply necessary heat flux in bare pipe and finned pipe.

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GNSS Precise Positioning Design for Intelligent Transportation System (지능형 교통시스템에 적합한 위성항법 기반의 정밀측위 구조 설계)

  • Lee, Byung-Hyun;Im, Sung-Hyuck;Heo, Moon-Beom;Jee, Gyu-In
    • Journal of Institute of Control, Robotics and Systems
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    • v.18 no.11
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    • pp.1034-1039
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    • 2012
  • In this paper, a structure of precise positioning based on satellite navigation system is proposed. The proposed system is consisted with three parts, range domain filter, navigation filter and position domain filter. The range domain filter generates carrier phase-smoothed-Doppler and Doppler-smoothed-code measurements. And the navigation filter calculates position and velocity using double-differenced code/carrier phase/Doppler measurements. Finally, position domain filter smooth position error, and it means enhancement of positioning performance. The proposed positioning method is evaluated by trajectory analysis using precise map date. As a result, the position error occurred by multipath or cycle slip was reduced and the calculated trajectory was in true lane.

Electrical properties for $ZnIn_2S_4$ epilayers grown by Hot Wall Epitaxy (Hot Wall Epitaxy (HWE)법에 의해 성장된 $ZnIn_2S_4$ 에피레이어의 전기적 특성)

  • Lee, Sang-Youl;Hong, Kwang-Joon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.143-144
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    • 2008
  • Single crystal $ZnIn_2S_4$ layers were grown on a thoroughly etched semi-insulating GaAs(100) substrate at $450^{\circ}C$ with the hot wall epitaxy (HWE) system by evaporating the polycrystal source of $ZnIn_2S_4$ at $610^{\circ}C$ prepared from horizontal electric furnace. The crystalline structure of the single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). The carrier density and mobility of single crystal $ZnIn_2S_4$ thin films measured with Hall effect by van der Pauw method are $8.51\times10^{17}$ electron/$cm^{-3}$, 291 $cm^2$/v-s at 293 K, respectively.

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Growth and electrical properties for $AgGaSe_2$ epilayers by hot wall epitaxy (Hot Wall Epitaxy(HWE)법에 의한 $AgGaSe_2$ 단결정 박막 성장과 전기적 특성)

  • Park, Chang-Sun;Hong, Kwang-Joon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.96-97
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    • 2008
  • Single crystal $AgGaSe_2$ layers were grown on thoroughly etched semi-insulating GaAs(100) substrate at 420 $^{\circ}C$ with hot wall epitaxy (HWE) system by evaporating $AgGaSe_2$ source at 630 $^{\circ}C$. The crystalline structure of the single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). The carrier density and mobility of single crystal $AgGaSe_2$ thin films measured with Hall effect by van def Pauw method are $9.24\times10^{16}cm^{-3}$ and 295 $cm^2/V{\cdot}s$ at 293 K, respectively.

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Thermosensitive Chitosan as an Injectable Carrier for Local Drug Delivery

  • Bae Jin-Woo;Go Dong-Hyun;Park Ki-Dong;Lee Seung-Jin
    • Macromolecular Research
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    • v.14 no.4
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    • pp.461-465
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    • 2006
  • Two types of injectable system using thermosensitive chitosan (chitosan-g-NIPAAm), hydrogel and microparticles (MPs)-embedded hydrogel were developed as drug carriers for controlled release and their pharmaceutical potentials were investigated. 5-Fluorouracil (5-FU)-loaded, biodegradable PLGA MPs were prepared by a double emulsion method and then simply mixed with an aqueous solution of thermosensitive chitosan at room temperature. All 5-FU release rates from the hydrogel matrix were faster than bovine serum albumin (BSA), possibly due to the difference in the molecular weight of the drugs. The 5-FU release profile from MPs-embedded hydrogel was shown to reduce the burst effect and exhibit nearly zero-order release behavior from the beginning of each initial stage. Thus, these MPs-embedded hydrogels, as well as thermosensitive chitosan hydrogel, have promising potential as an injectable drug carrier for pharmaceutical applications.

Behavior of Synthetic Layered Double Hydroxides in Soils (인공합성된 Layered Double Hydroxides의 토양중 행동)

  • Choi, Choong-Lyeal;Seo, Yong-Jin;Lee, Dong-Hoon;Kim, Jun-Hyeong;Yeou, Sang-Gak;Choi, Jyung;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.5
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    • pp.412-417
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    • 2007
  • This study was to elucidate the effects of layered double hydroxides(LDHs) application on the chemical properties of the soils along with the fate of the applied LDHs. The effects of LDHs application were compared with those of calcium carbonate widely used for the neutralization of acidic soils. Incorporation of LDHs into the soil resulted in higher pH value and $Mg^{2+}$ content in soil leachate than that of $CaCO_3$ treatment. There was no significant difference in water-soluble P content in both the treatments. However, $Al^{3+}$ and $Si^{4+}$ contents were decreased by LDHs and $CaCO_3$ treatment, even though a large amount of $Al^{3+}$ was released into soil solution with the disintegration of LDHs framework. LDHs structure in soil was gradually disintegrated from the its original layered structure under acidic condition of soil. Therefore, this study suggests that LDHs could be utilized as a carrier of functional substances to enhance the efficiency of various ago-chemicals without any ill effects on the soil environments.

Development of On-Line Type Voltage Sag Compensation Systems by Using a Supercapacitor (수퍼커패시터를 이용한 상시가동형 순시전압강하 보상시스템의 개발)

  • Shon, Jin-Geun
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.58 no.2
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    • pp.101-107
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    • 2009
  • This paper deal with development of on-line type voltage sag compensation system using supercapacitor EDLC to solve the voltage sag problems which are considered to be dominant disturbances affecting the power quality. With the wide use of semiconductor devices in electrical equipment, modem-type loads are becoming increasingly sensitive to the voltage sags and the disturbances prove to be costly to industries. Supercapacitor EDLC is employed to compensate dynamically for the voltage sag of system with sensitive loads. This capacitor has higher energy density than the electrolytic capacitor. Also, this capacitor has a lot of advantage such as no maintenance, longer life cycle and faster charge-discharge time than the battery system. Therefore, in this paper, the energy design scheme of supercapacitor and the configuration technique of on-line type voltage sag compensation systems are newly introduced. According to the results of experimental of prototype 5[kVA] system, it is verified that the developed system has effectiveness of voltage sag compensation by using a supercapacitor EDLC.