• 제목/요약/키워드: Donguibogam

검색결과 272건 처리시간 0.034초

굼벵이 추출물의 RAW 264.7 세포에 대한 면역증강 효과 (Immuno-enhancing Effect of Protaetia brevitarsis seulensis (white grub) Extracts on RAW 264.7 Cell Line.)

  • Park, Bog-Im;Seo, Jae-Bin;Jin, Yu-Mi;Kim, Seong-Seon;Sim, Hyeon-Jae;Lee, Hoon-Yeon;Kim, Seong-Oh;kim, Dong-Keun;Jo, Mi-Na;Cho, Yong-Jin;Kim, Chong-Tai;Kim, Tae-Eun;Jeon, Yong-Deok;Jin, Jong-Sik
    • 한국자원식물학회:학술대회논문집
    • /
    • 한국자원식물학회 2018년도 추계학술대회
    • /
    • pp.102-102
    • /
    • 2018
  • Protaetia Brevitarsis Seulensis(white grub) has been traditionally used as medicinal stuff to treat blood stasis, occlusion of menstruation, tetanus and liver cancer in Asian countries (Korea, Japan, China, Taiwan, India and Myanmar). Especially, Donguibogam, which is traditional korean medicinal book, described the white grub as traditional medicine to treat hepatic diseases and vascular disorders. The white grub has been considered as highly nutritional food. The major constituents of white grub are rich in protein, healthy fats, iron, calcium. Recent studies announced that white grub has hepatoprotective effect and anti-microbacterial effect. However, the immuno-enhancing effect of white grub extracts in RAW 264.7 macrophage cells has not been studied yet. In this study, the various concentrations of white grub extract were examined to find immuno-enhancing effects on RAW 264.7 cells. Cytotoxicity was determined by MTT assay and immuno-enhancing effect of white grub extract was investigated by measuring nitric oxide (NO) production compared with only lipopolysaccharide (LPS) treatment. White grub extracts (0.001 - 10 mg/ml) did not show cytotoxicity. Additionally, white grub extracts (0.001 - 1mg/ml) had Immuno-enhancing effect on RAW 264.7 cells compared with only LPS treated group. These results might be provided proof to develop beneficial immuno-enhancing material for human health.

  • PDF

인체 간암세포에서 비기환(肥氣丸), 대칠기탕(大七氣湯) 및 목향빈랑환(木香檳榔丸)의 항암 활성 비교 (The Anti-cancer Effects of Bigihwan, Daechilgithang, and Mokwhyangbinranghwan Ethanol Extracts in Human Hepatocellular Carcinoma Cells)

  • 김소영;홍수현;최성현;정재훈;최영현
    • 생명과학회지
    • /
    • 제30권5호
    • /
    • pp.460-467
    • /
    • 2020
  • 간암은 전 세계적으로 가장 높게 진단되는 암 중 하나이며, 방사선 및 화학 요법이 일반적으로 사용되는 치료법이지만 다양한 부작용은 치료 효과를 크게 제한한다. 따라서 전통 의학에서 사용되어 온 처방법은 이를 극복할 수 있는 대안이 될 수 있다. 본 연구에서는 동의보감에 기술되어 있는 3가지 한약 처방전(비기환, 대칠기탕 및 목향빈랑환)을 선택하여 인체 간암세포에 대한 항암 효과를 평가하였다. 간암세포에서 3가지 처방전의 에탄올 추출물의 세포 독성을 조사하기 위한 MTT 분석 결과, 비기환 추출물은 대칠기탕 및 목향빈랑환에 비하여 세포 생존력을 현저하게 억제하였다. 그리고, flow cytometry 분석의 결과에서 3가지 처방전 추출물에 의한 간암세포의 증식 억제가 apoptosis 및 autophagy 유도와 관련이 있었다. 특히, 비기환 추출물은 미토콘드리아의 기능을 크게 손상시켰으며 다른 두 처방전과 비교하여 mitophagy 유발 가능성을 보여주었다. 아울러 비기환 추출물은 LC3의 발현을 현저하게 증가시켰으며, 이는 Bcl-2의 발현 감소를 동반하는 반면, Bax의 발현에 영향을 미치지 않았다. PINK1의 발현 또한 비기환 추출물이 처리된 세포에서 매우 증가하였다. 나아가, autophagy 억제제는 3가지 처방전 추출물 처리에 의한 세포 생존율 감소와 apoptosis 유도를 억제하였으며, 이러한 결과는 이들 처방전 추출물 처리에 의한 autophagy가 apoptosis 개시에 관여하고 있음을 보여주는 것이다. 결론적으로, 본 연구 결과는 비기환 추출물이 3가지 처방전 중에서 가장 높은 항암 활성을 보였으며, 비기환 추출물은 autophagy 유도제로서 간암세포의 증식을 억제함을 의미한다.

『東醫寶鑑』의 熟地黃 제조방법 (How to Prepare Rehmanniae Radix Preparata Described in the 『Treasured Mirror of Eastern Medicine』)

  • 노종성;윤미정;신순식
    • 대한한의학방제학회지
    • /
    • 제24권1호
    • /
    • pp.17-30
    • /
    • 2016
  • Objectives Rehmanniae Radix Preparata was prepared in the traditional Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine(Donguibogam)』 with a view to measuring the contents of 5-Hydroxymethyl-2-furaldehyde(5-HMF) at individual stages of steaming and sundrying and identifying new chemical components.Methods Based on the traditional Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』, Rehmanniae Radix Preparata steamed and sundried once through nine times was prepared. Thereafter, 5-HMF contents were analyzed and new chemical components were identified in the Rehmanniae Radix Preparata using Waters HPLC e2695, 2640 detectors, a Waters Acquity UPLC system, and a Micromass Q-TOF Premier mass spectrometer.Results The Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』 is a unique preparation method in Republic of Korea different from that in China. In the first stage of the method, fresh Rehmanniae Radix Crudus was divided into high quality, medium quality, and low quality ones named Rehmanniae Radix Crudus (Caelum)(天黃), Rehmanniae Radix Crudus (Homo)(人黃), and Rehmanniae Radix Crudus (Terra)(地黃) respectively to use Rehmanniae Radix Crudus (Caelum) and Rehmanniae Radix Crudus (Homo) for preparation of juice while using Rehmanniae Radix Crudus (Terra) to make Rehmanniae Radix Preparata. In the second stage, Rehmanniae Radix Crudus (Caelum) and Rehmanniae Radix Crudus (Terra) were made into juice and Rehmanniae Radix Crudus (Terra) was soaked in the juice. In the third stage, among auxiliary materials, rice wine named Purum Vinum Oryzae(淸酒) brewed from sticky rice was sprinkled on Rehmanniae Radix Crudus (Terra) to the extent that Rehmanniae Radix Crudus (Terra) became wet. In the fourth stage, Rehmanniae Radix Preparata steamed in earthenware steamer was dried under natural sunlight. The contents of 5-HMF in Rehmanniae Radix Preparata steamed and sundried once through nine times were shown to be below 0.1% in all cases. Pomolic acid was identified as a new chemical component.Conclusions In conclusion, the Rehmanniae Radix Preparata preparation method set forth in the 『Treasured Mirror of Eastern Medicine』 is thought to be a unique preparation method in South Korea in which Rehmanniae Radix Preparata is completed through the first stage in which fresh Rehmanniae Radix Crudus collected from fields is divided into high, medium, and low quality ones and fresh Rehmanniae Radix Crudus juice is made, the second stage in which the high quality fresh Rehmanniae Radix Crudus is soaked in the fresh Rehmanniae Radix Crudus juice, the third stage in which the fresh Rehmanniae Radix Crudus is steamed, and the fourth stage in which the steamed Rehmanniae Radix Crudus is dried.

기능성 소화불량증의 허실진단에 대한 위전조의 유용성 (The Usefulness of Electrogastrography on the Differential Diagnosis of Deficiency or Excess Condition in Patients with Functional Dyspepsia)

  • 유종민;임중화;한숙영;장선영;김현경;이준석;김진성;류봉하;류기원;윤상협
    • 대한한방내과학회지
    • /
    • 제25권4호
    • /
    • pp.346-355
    • /
    • 2004
  • Background & Object : The aim of this study was to investigate the usefulness of electrogastrography on differential diagnosis of deficiency or excess Condition in patients with functional dyspepsia Methods : Selected symptoms for diagnosis of deficiency and excess in functional dyspepsia have been reviewed in some literatures, notably within Oriental medicine, Donguibogam and Gogeumdoseojipseong-uibujeolrok. This is what was investigated in this study. 93 patients (male 32, female 61) were divided into three groups; Non-pain group, Pre-treatment pain group (chief complain was abdominal pain at the first medical examination), and Mid-treatment pain group(they had no pain at the time of first medical examination, but showed abdominal pain within two weeks). 10 healthy people participated as normal controls. Gastric motility were recorded and analyzed using electrogastrography during fasting and postprandial period. In assessment, effectiveness was divided into validity, sensitivity and specificity. Results : Epigastric pain reflected the tendency for excess condition in comprehensive diagnosis, which was linked with postprandial arrhythmia in electrogastrography(p=0.001). Postprandial arrhythmia detected abdominal pain with a validity of 78.6%, a sensitivity of 79.2%, and a specificity of 78%(p<0.001). Conclusions : Results 1Tom Postprandial arrhythmia in electrogastrography support that the index of excess condition in the comprehensive diagnosis of symptoms is useful for patients with functional dyspepsia.

  • PDF

경험의안(經驗醫案) 『우잠잡저(愚岑雜著)』의 간울(肝鬱) 치험례(治驗例) (Analysis of Examples of Treating Ganwul[stagnation of liver qi] in 『WooJam JabJeo(愚岑雜著)』)

  • 박상영;오준호;권오민;안상영;안상우
    • 한국의사학회지
    • /
    • 제25권1호
    • /
    • pp.53-60
    • /
    • 2012
  • In recent times, a medical book called "WooJam JabJeo" has been published in complete Korean version. Not a few cases where the vivid appearance of the place for medical treatment are included intact in this medical book. This study is attempting to analyze one symptom of a disease by choosing it among the diseases our ancient sages treated in the light of the fact that there are only a few models which are applied to today's clinical treatment by our ancient sages appearance of medical service. This study believes that such an analysis of ancient medicine could be a part of the efforts to increase the applicable models to today's clinical treatment one by one. This paper is aimed at introducing and analyzing the three cases of Ganwul which are found in "WooJam JabJeo". It's because this study thinks that Ganwul is a symptom over which oriental medicine can not only establish dominance over western medicine it its diagnosis and prescription but Ganwul will also have high applicability to today's clinical treatment. Through this research, this study was able to perceive that the author. Jang, Tae-kyeong of "WooJam JabJeo" had vast knowledge of medical theories, especially in author's understanding diseases and composing prescription based on "Donguibogam". Up to the present time, domestic medical circles have understood the emergence of "Jejungshinpyeon(濟衆新編)"(1799) or "Bangyakhappyeon(方藥合編)"(1885) as an outlining work at most for overcoming such a limit of "DonguiBogam" superficially. However, by looking into Jang Tae-kyeong's understanding of "Dongui Bogam". this study was able to know the fact that the emergence of such medical books was possible because the medical knowledge included in "Dongui Bogam" was fully understood and digested in society of the Josen Dynasty. Conclusively, such a fact reminds us that it serves as a momentum to confirm once more that "Dongui Bogam" could be a good model for even today's clinical treatment.

동의보감(東醫寶鑑)에 수록(收錄)된 인삼(人蔘)이 주약(主藥)으로 배오(配伍)된 방제(方劑)의 활용(活用)범위, 병증, 주치(主治), 병리(病理) 및 구성내용(構成內容) 조사(調査) (Studies on Therapeutic range, Symptom, Pathology, and composition of Ginseng Radix -main blended Prescriptions from Donguibogam)

  • 조대연;정종길;윤용갑
    • 대한한의학방제학회지
    • /
    • 제9권1호
    • /
    • pp.35-82
    • /
    • 2001
  • In the Encyclopedia Medica Koreana(Dongeuibogam), I have researched 245 prescriptions in which Panax Ginseng plays an important role. And I have got the following results. The healing scope and frequency of ginseng-mainly-included prescriptions are Child Part 29(11.83%), Violent Cough Part 23(9.38%), Sick-by-Cold Part 21(8.57%), Oncosis Part 16(6.53%), Overwork Part 14(5.71%), Gynecologic Part 14(5.71%), Internal Part 13(5.3%), Apoplexy Part 11(4.48%), Mind Part 10(4.08%) and Fecal Part 10(4.08%) prescriptions. And also each of Nausea Part, Anger Part, and Spirit parts has the same 5 (2.04%) prescriptions. And each of Qi Part, Diabetes Meatus Part, Malaria Part, and Humoral Part has 4(1.63%) prescriptions. And each of Foot Part, Choleraic Part, Genital Part, Blood Part, and Voice Part has 3 (1.2%). All of these prescriptions cover 88.88%. And besides listed parts above, Panax Ginseng is all used in 48 Parts: Body-Mind Part. Mouth-Tongue Part, Breast Part, Muscle Part, Swelling Part, Urine Part, Epidermis Part, Heat Part, Anus Part, Stomach Part, Eye Part, Laryngopharynx Part. Uterus Part" Heavy Stomach Part, Head Part, Pulse Part, Hair Part, Navel Part, Emetic Part, Costal Part, Edema Part, Vomiting Part, Superstitious Part, and Cardiac Part, etc. Of the prescriptions in which Panax Ginseng plays an important role, the most representative diseases, which more than 86.8% prescriptions cure, are shock, numbness from cold, Taeeum disease, oncosis, overwork, sick from eating, numbness of extremities, diarrhea, tachycardia, forgetfulness, nausea, heat from kidney, nocturnal emission, short breath, diabetes meatus, malaria, sweating, sweating overnight, beriberi, cholera, insomnia from enervation, sialitis, navel pain, hemorrhage, and loss of voice. The pathology of the prescriptions in which Panax Ginseng plays an important role is divided into the organ problems, six natural factors, seven extreme feelings, unbalanced humoral status, overwork, and, unbalance of qi and blood. Spleen, heart, and uterus is the main cause of organ problems; wind and cold are the main cause of six natural factors; heavy humors are the main cause of unbalanced humoral status; the stasis of seven feelings are the main cause of seven extreme feelings; the lack of stamina and overwork are the main cause of the overwork; the lack of qi, the lack of blood, and, the lack of qi and blood are the main cause of the unbalance of qi and blood. After I have researched the contents of the prescriptions in which Panax Ginseng plays an important role, I could understand the addition of the different prescriptions, combination of medicines, and the role of medicine groups associated with Panax Ginseng. So from now on, the results I have got could be used as the data which show the theoretical basis on the prescriptions.

  • PDF

가지-청열소독음(淸熱消毒飮)의 항산화 및 항염 효능에 관한 연구 (Study on Anti-oxidant and Anti-inflammatory Activity of Eggplant-cheongyeolsodokum)

  • 윤종문;김동인;이지혜;한소정;김하은;김현정;남규우;박지연;지경엽;안봉전
    • 대한화장품학회지
    • /
    • 제43권2호
    • /
    • pp.125-135
    • /
    • 2017
  • 본 연구의 목적은 8가지 한약재(가지, 금은화, 감초, 천궁, 당귀, 황련, 치자, 연교)로 구성된 가지청열소 독음의 항산화 효과와 항염증 효과를 검증하여 효과적인 화장품소재로서의 가능성을 확인하는 것이다. 한약재의 구성과 비율은 동의보감에 수록된 청열소독음(淸熱蘇毒飮) 처방을 변형시켜 사용하였고 추출은 열수와 70% 에탄올로 하여 동결건조 분말화하였다. 항산화 효능을 확인하기 위해 라디칼 소거능력(DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)유사활성능력, 총 폴리페놀 화합물 함량을 조사하였고 항염증 효능을 확인하기 위해 LPS (lipopolysaccharide)로 염증반응을 유도시킨 RAW264.7 대식세포에서의 nitric oxide(NO)생성 저해력과 western blot 분석을 통한 염증 관련 단백질 inducible nitric oxide synthase (iNOS)와 cyclooxygenase (COX-2)의 발현 저해를 확인하였다. 그 결과, 가지청열소독음은 뛰어난 항산화 효과와 항염증 효과를 나타내었고 화장품을 위한 효과적인 성분으로 사용 가능할 것으로 사료된다.

전통한지의 제조 기술 및 우수성에 관한 논고(論考) (A study on manufacturing technologies and excellence of Korean traditional paper)

  • 정선화
    • 헤리티지:역사와 과학
    • /
    • 제48권1호
    • /
    • pp.96-131
    • /
    • 2015
  • 한지는 우리의 옛 선조들의 뛰어난 기술과 장인정신으로 오늘날에도 그 가치와 기술을 높게 평가받고 있는 귀중한 문화유산으로 이는 그 시대의 장인정신과 축적된 기술, 한지제조에 적합한 천혜의 자연조건인 닥섬유와 물과 햇빛과 더불어 환경친화적이고 보존성이 우수한 천연자숙제 및 점질물 등이 잘 어우러진 산물이라고 할 수 있다. 한지는 닥나무 껍질(저피(楮皮))을 가공하여 손으로 만든 종이를 일컫는 것으로 조선 말엽 일본을 통하여 서양식 기계로 제조한 종이가 생산되면서 그간의 종이는 한지라 하고 서양의 종이를 양지라 구분하여 부른다. 한지는 재료 및 초지방법에 따라 다양하게 불리고 사용되어져 왔으며, 그 기능 또한 다양하게 활용되어져 왔다. 또한 한지는 과거 삼국시대부터 조선 시대에 이르기까지 동양 3국(한국, 중국, 일본) 중에서 독특하고 우수한 품질로 각광을 받아왔고, 오늘날 한지 특유의 우수한 보존성으로 수많은 국가지정 지류문화재들이 지금까지 잘 전승되고 있다. 아울러 세계적으로 그 가치를 인정한 UNESCO 세계기록유산에 한국은 모두 11건이 등재되었는데 그 중 전통한지로 구성되어 있는 기록물은 8건으로 "훈민정음, 조선왕조실록, 직지심체요절, 승정원일기, 조선왕조의궤, 동의보감, 일성록, 난중일기" 등이 이에 해당된다. 이러한 한지의 우수한 보존과학적 특징을 규명하기 위하여 다양한 연구 사업들이 진행되어져 왔다. 한지에 대한 분석기술과 제조기술개발, 복원기술연구 등을 통하여 우리 전통한지에 대한 우수성을 과학적으로 재입증하고 우리나라를 대표하는 전통문화유산의 하나로써 그 명맥을 유지해 나가야할 것이다.

조선 왕실 가례의 부용향(芙蓉香) 연구 - 『순조순원왕후가례도감의궤』를 중심으로 - (A Study on the Use of Buyonghyang During the Joseon Royal Wedding Ceremony - Focused on Sunjo Sunwonwanghoo Garyedogam Uigwe -)

  • 하수민
    • 헤리티지:역사와 과학
    • /
    • 제52권3호
    • /
    • pp.222-239
    • /
    • 2019
  • 본 연구는 문헌을 통해 조선왕실의 부용향을 "순조순원왕후가례도감의궤"의 사례를 중심으로 살펴본 것이다. 한국에서 향은 삼국시대부터 불교문화와 함께 전래되었다. 부용향은 조선시대 왕실을 상징하던 향이며 왕실의 각종 의례에 사용되었다. 민가에서도 혼인할 때 신부의 가마 앞에서 부용향을 태우기도 했다. 부용향은 의례, 방향, 방충, 의료의 목적으로 다양하게 사용되었다. 부용향은 침속향과 백단향을 주 향재로 하여 모향, 백급, 영릉향, 감송향, 팔각향, 삼내자, 정향, 소뇌, 비초 등 10가지 향재를 혼합해 만든 합향이다. "탁지준절"과 "세종실록지리지", "조선왕조실록"의 기록을 통해 향재의 수급 방법을 추적했다. 각 향재의 특성을 파악하고 "제중신편", "동의보감" 등의 의서를 통해 제조법을 살펴보았다. 그리고 "조선왕조실록'과 "승정원일기"의 기록을 통해 부용향을 제작한 향장의 협업과 처우를 살펴보았다. 가례에서 부용향이 사용된 사례를 통해 조선시대에 향이 갖던 의의를 알아보았다. 가례는 이전의 가례도감의궤에 비하여 그 체제가 잘 정비된 것으로 평가받는 "순조순원왕후가례도감의궤"를 참고했다. 궁에서는 의례를 진행하는 동안 항상 향을 사용했다. 반면 신부의 집에서 진행된 경우 오로지 비수책의에서만 향로 차비 등의 향과 관련된 차비가 동원되어 이 시기에만 향을 사용했을 것으로 추정할 수 있다. 책봉 후에야 비로소 향을 사용하게 됨을 통해 부용향은 왕실 사람만이 사용했으며 왕실을 상징하는 향이었음을 추측할 수 있다.

TNF-α로 유도된 혈관내피세포의 혈관염증에 미치는 오적산(五積散)의 억제 효과 (Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells)

  • 한병혁;윤정주;김혜윰;안유미;홍미현;손찬옥;나세원;이윤정;강대길;이호섭
    • 대한본초학회지
    • /
    • 제33권4호
    • /
    • pp.59-67
    • /
    • 2018
  • Objectives : Ojeoksan, originally recorded in an ancient Korean medicinal book named "Donguibogam" and has been used for the treatment of circulation disorder of blood which was called blood accumulation (血積) in Korean medicine. Therefore, this study was carried out to investigate the beneficial effect of OJS on vascular inflammation in HUVECs. Methods : We evaluated the effect of OJS on the expression of cell adhesion molecules and protective role in HUVEC stimulated by TNF-${\alpha}$ by using Western blot. Results : Pretreatment with OJS decreased the adhesion of HL-60 cells to TNF-${\alpha}$-induced HUVEC. OJS suppressed TNF-${\alpha}$-induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and endothelial cell selectin (E-selectin). Moreover, OJS significantly decreased TNF-${\alpha}$-induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of $I{\kappa}B-{\alpha}$ in the cytoplasm compared to the experimental group. Pretreatment with OJS inhibited the trans-location of NF-${\kappa}B$ p65 to the nucleus. OJS also inhibited phosphorylation of MAPKs compared to the experimental group. OJS significantly increased the protein expression of Nrf2 and HO-1. Conclusions : Ojeoksan has a protective effect on vascular inflammation, and might be a potential therapeutic agent for early atherosclerosis.