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http://dx.doi.org/10.6116/kjh.2018.33.4.59.

Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells  

Han, Byung Hyuk (Department of Physiology, College of Korean Medicine)
Yoon, Jung Joo (Department of Physiology, College of Korean Medicine)
Kim, Hye Yoom (Department of Physiology, College of Korean Medicine)
Ahn, You Mee (Department of Physiology, College of Korean Medicine)
Hong, Mi Hyeon (Department of Physiology, College of Korean Medicine)
Son, Chan Ok (Department of Physiology, College of Korean Medicine)
Na, Se Won (Department of Physiology, College of Korean Medicine)
Lee, Yun Jung (Department of Physiology, College of Korean Medicine)
Gang, Dae-Gil (Department of Physiology, College of Korean Medicine)
Lee, Ho Sub (Department of Physiology, College of Korean Medicine)
Publication Information
The Korea Journal of Herbology / v.33, no.4, 2018 , pp. 59-67 More about this Journal
Abstract
Objectives : Ojeoksan, originally recorded in an ancient Korean medicinal book named "Donguibogam" and has been used for the treatment of circulation disorder of blood which was called blood accumulation (血積) in Korean medicine. Therefore, this study was carried out to investigate the beneficial effect of OJS on vascular inflammation in HUVECs. Methods : We evaluated the effect of OJS on the expression of cell adhesion molecules and protective role in HUVEC stimulated by TNF-${\alpha}$ by using Western blot. Results : Pretreatment with OJS decreased the adhesion of HL-60 cells to TNF-${\alpha}$-induced HUVEC. OJS suppressed TNF-${\alpha}$-induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and endothelial cell selectin (E-selectin). Moreover, OJS significantly decreased TNF-${\alpha}$-induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of $I{\kappa}B-{\alpha}$ in the cytoplasm compared to the experimental group. Pretreatment with OJS inhibited the trans-location of NF-${\kappa}B$ p65 to the nucleus. OJS also inhibited phosphorylation of MAPKs compared to the experimental group. OJS significantly increased the protein expression of Nrf2 and HO-1. Conclusions : Ojeoksan has a protective effect on vascular inflammation, and might be a potential therapeutic agent for early atherosclerosis.
Keywords
Ojeoksan; HUVEC; TNF-${\alpha}$; adhesion molecules; NF-${\kappa}B$; reactive oxygen species; HO-1;
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