• 제목/요약/키워드: Dongbu

검색결과 376건 처리시간 0.021초

GIS기반 홍수위험도 평가를 통한 공간정보 구축 방안 개발 (Development of Strategics for Establishment of Spatial Information by Assessment of GIS-Based Flood Risk)

  • 심규성;이춘호;이태근;지계환
    • Spatial Information Research
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    • 제23권2호
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    • pp.39-48
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    • 2015
  • 본 연구에서는 2차원 범람해석을 통해 도출된 침수해석정보에 현실적인 위험도를 고려한 산정식을 적용하여 홍수위험도를 평가하였고, 이를 표준화된 정보체계로 제공하는 방안을 제시하였다. 일반적으로 침수심과 침수구역을 기반으로 하는 현재의 홍수위험도 평가방법은 홍수발생시 사람에게 미치는 실질적인 위험도를 충분히 반영하지 못 할 것으로 판단되어 침수심, 유속, Debris Factor와 Flood Hazard Rating 관계식을 이용하여 외수범람이 사람에게 미치는 홍수위험도를 평가하고 4개로 등급화하였다. 이를 위해 2차원 외수범람해석 모형인 Flumen을 이용하여 대구의 금호강유역 대하여 500년 빈도 홍수로 인한 월류와 200년 빈도 홍수로 인한 파제 시나리오에 따라 외수범람을 모의하였다. 이러한 연구결과를 통해 외수범람시 침수가 예상되는 지역에 위치한 사람들에게 더 현실적인 위험도정보를 제공하고, 표준화된 정보체계 기반에서 위험도정보 제공이 가능해짐으로써 재해관련분야의 정보분석, 평가, 계획수립 등 다양한 분야에서 활용성을 증대시킬 수 있을 것으로 판단된다.

Postharvest Disease Control of Colletotrichum gloeosporioides and Penicillium expansum on Stored Apples by Gamma Irradiation Combined with Fumigation

  • Cheon, Wonsu;Kim, Young Soo;Balaraju, Kotnala;Kim, Bong-Su;Lee, Byeong-Ho;Jeon, Yongho
    • The Plant Pathology Journal
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    • 제32권5호
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    • pp.460-468
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    • 2016
  • To study the control of postharvest decay caused by Colletotrichum gloeosporioides and Penicillium expansum, gamma irradiation alone or in combination with fumigation was evaluated to extend the shelf life of apples in South Korea. An irradiation dose of 2.0 kGy resulted in the maximum inhibition of C. gloeosporioides and P. expansum spore germination. The gamma irradiation dose required to reduce the spore germination by 90% was 0.22 and 0.35 kGy for C. gloeosporioides and P. expansum, respectively. Microscopic observations revealed that when the fungal spores were treated with gamma irradiation (4.0 kGy), conidial germination was stopped completely resulting in no germ tube formation in C. gloeosporioides. Treatment with the eco-friendly fumigant ethanedinitrile had a greater antifungal activity against C. gloeosporioides and P. expansum in comparison with the non-treated control under in vitro conditions. The in vitro antifungal effects of the gamma irradiation and fumigation treatments allowed us to further study the effects of the combined treatments to control postharvest decay on stored apples. Interestingly, when apples were treated with gamma irradiation in combined with fumigation, disease inhibition increased more at lower (< 0.4 kGy) than at higher doses of irradiation, suggesting that combined treatments reduced the necessary irradiation dose in phytosanitary irradiation processing under storage conditions.

Development of New Molecular Markers for the Identification of Male Sterile Cytoplasm in Peppers (Capsicum annuum L.)

  • Min, Woong-Ki;Kim, Byung-Dong;Kim, Sung-Gil;Lee, Sang-Hyeob
    • 원예과학기술지
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    • 제29권1호
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    • pp.53-60
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    • 2011
  • Cytoplasmic male sterility (CMS) induced by mutant mitochondria genome, has been used for commercial seed production of $F_1$ hybrid cultivars in diverse crops. In pepper (Capsicum annuum L.), two sterile cytoplasm specific gene organization, atp6-2 and coxII were identified. An open reading frame, orf456 nearby coxII gene has been speculated to induce male sterility (MS) by mutagenic analysis. Moreover, molecular markers for atp6-2 and coxII of mitochondrial genotype (mitotype) were developed. However, the Cytoplasmic MS specific markers, atp6SCAR and coxIISCAR markers appeared in both N and S cytoplasms when polymerase chain reaction (PCR) cycles prolonged more than 40 cycles. Since the reported molecular markers were dominant markers, the presence of the faint sterile-specific band in normal cytoplasm may lead to the mis-classification of pepper breeding lines. To solve this problem, one common forward primer and two different reverse primers specific to normal coxII and sterile orf456 genes were designed after analyzing their gene organizations. By using these three primers, N and S coxII specific bands were co-amplified in male-sterile lines, but only normal coxII specific band was amplified in maintainer lines. Since the reverse primer for sterile coxII was specifically designed 275 bp downstream of orf456, relatively stable PCR amplification patterns were observed regardless of the number of PCR cycles. These primer sets easily identified different mitotypes among the divergent breeding lines, commercial cultivars and diverse germplasms.

Development of Gene-based Markers for the Allelic Selection of the Restorer-of-fertility Gene, Rfo, in Radish (Raphanus sativus)

  • Kim, Sunggil;Lim, Heerae;Cho, Kang-Hee;Park, Pue Hee;Park, Suhyung;Sung, Soon-Kee;Oh, Daegeun;Kim, Ki-Taek
    • 한국육종학회지
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    • 제41권3호
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    • pp.194-204
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    • 2009
  • Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for $F_1$-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in $F_1$-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating $F_1$-hybrid cultivar development in radish.

Comparison of Biological Activities of Dendropanax morbiferus by Different Cultivation Areas in Korea

  • Oh, Junseok;Hong, Jae-Heoi;Park, Tae-Young;Jeon, Byeonggyu;Ha, Neul-I;Kim, Kyung Je;Jin, Seong Woo;Koh, Young Woo;Im, Seung Bin;Jeong, Hee Gyeong;Seo, Kyoungsun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.105-105
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    • 2019
  • Dendropanax morbifera H.Lev is an evergreen tree that lives in subtropical climates. About 75 species of D. morbifera live in East Asia, but only one species live in Korea(Korean dendropanax). D. morbifera belonging to Aralicacae family 98% of D. morbifera are distributed in Jeollanam-do in the Korea and the grow wild in Gyeongsangnam-do and Jeju Island. The harvest time and usage of D. morbifera were recorded in traditional medicinal books. The roots and stems of D. morbifera had been used for traditional medicine to treat migraine, menstrual irregularity and skin disease. And D. morbifera leaves are contain flavonoids and polyacetylene compounds. In this study, we were investigated the physiological activity of D. morbifera by different areas collected at the same time, and compared to characteristics of plants. D. morbifera collected from Jeollanam-do (Goheung-gun), Gyeongsangnam-do (Namhae-gun) and Jeju Island, and dried at $50^{\circ}C$ for three days. We used dried D. morbifera powder for antioxidant tests. Each sample was extracted with hot water under the same conditions. The contents of total polyphenols and total flavonoids from D. morbifera were identified. Also, we performed to DPPH radical scavenging activity, ABTS cation radical scavenging activity and Superoxide anion scavenging activity efficacy for antioxidant activity determination. The contents of total polyphenols and total flavonoids in hot water extract of D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do were higher than Jeju. However, D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do showed no significant difference those content of total polyphenols and total flavonoids. And the antioxidant capacity was showed the similar patterns in antioxidant activity.

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Utilization of the bar gene to develop an efficient method for detection of the pollen-mediated gene flow in Chinese cabbage (Brassica rapa spp. pekinensis)

  • Lim, Chaewan;Kim, Sunggil;Choi, Yeonok;Park, Young-doo;Kim, Sung Uk;Sung, Soon-Kee
    • Plant Biotechnology Reports
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    • 제1권1호
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    • pp.19-25
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    • 2007
  • To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2-3 leaf stage were most vulnerable to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the bar gene in T1 segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions. However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for genetically modified (GM) Chinese cabbage cultivars.

Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry

  • Kim, John;Ji, Hye-Young;Lee, Seung-Seok;Yoo, Sung-Eun;Kim, Sun-Ok;Lee, Dong-Ha;Lim, Hong;Lee, Hye-Suk
    • Archives of Pharmacal Research
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    • 제25권5호
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    • pp.664-668
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    • 2002
  • KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of KR-31543 in the presence of NADPH resulted in the formation of a metabolite M1. M1 was identified as N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amine on the basis of LC-MS/MS analysis with the synthesized authentic standard. Rat CYP3A1 and 3A2 are the major CYP isozymes involved in the formation of M1.

Effects of a New Neuroprotective Agent KR-31378 on Liver Cytochrome P450s in Male Sprague Dawley Rats

  • Jeong, Tae-Cheon;Kim, Ji-Young;Ji, Hye-Young;Lee, Dong-Ha;Kim, Sun-Ok;Lim, Hong;Yoo, Sung-Eun;Lee, Hye-Suk
    • Archives of Pharmacal Research
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    • 제26권10호
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    • pp.800-804
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    • 2003
  • The effects of KR-31378, a neuroprotective agent for ischemia-reperfusion damage, on liver microsomal cytochrome P450s (CYPs) were investigated in male Sprague Dawley rats. When rats were treated orally with KR-31378 for 7 consecutive days, CYP3A-selective erythromycin N-demethylase (ERDM) activity was significantly induced in a dose-dependent manner. In Western immunoblotting, CYP 3A proteins were clearly induced by treatment with KR-31378. Within 24 h after treatment with 80 mg/kg of KR-31378, ERDM activity was induced in liver microsomes in accompanied by induction of the level of CYP 3A proteins. The present results suggest that KR-31378 might modulate the expression of CYP 3A enzymes in humans.

세무계획측면에서 분석한 동부그룹 물적분할 (Does Tax Really Matter in Planning the Dongbu Group's Spin-Offs?)

  • 전병욱;조형태
    • 기업가정신과 벤처연구
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    • 제20권1호
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    • pp.1-18
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    • 2017
  • 본 연구는 물적분할에 대한 법인세 과세제도가 개정된 2010년 7월을 전후해서 다수의 물적분할을 진행한 동부그룹의 구체적인 사례를 바탕으로 모든 거래당사자들의 관점에서 물적분할의 당사자인 분할법인과 분할신설법인의 법인세 부담이 최소화되도록 물적분할의 유형을 선택하는 거래를 구성했는지에 대하여 분석하였다. 2010년 6월 이전에 적용된 제1기 과세제도에서는 과세이연요건을 만족하는 적격물적 분할의 경우에 분할법인의 자산양도차익에 대한 과세이연을 통해 비적격물적분할에 비해 법인세 부담이 작아지는 것을 확인할 수 있다. 이에 반해 2010년 7월 이후부터 2011년 말까지 적용된 제2기 과세제도에서는 적격물적분할의 경우에 양도차익에 대해 분할법인과 분할신설법인에게 각각 장래의 주식처분시점과 자산의 처분 및 상각시점에서 과세함으로써 현재가치를 고려하면 비적격물적분할에 비해 상대적으로 세금부담이 커질 수도 있다. 본 연구에서 분석한 동부그룹의 4건의 분할 사례 중에서 1건((주)동부한농 사례)은 제1기 과세제도에서 이루어진 것이고, 3건((주)동부케미칼, (주)동부익스프레스 및 동부특수강(주) 사례)은 제2기 과세제도에서 이루어진 것인데, 조세비용의 측면에서 분석하면 (주)동부한농 분할사례는 적격분할일 것으로 예상되고, 나머지 3건의 분할사례는 구체적인 할인율 및 처분기간 등을 적용하면 비적격분할일 것으로 예상된다. 그러나, 해당 기업들의 합병 관련 공시 내용을 분석하면 이들 분할사례에서는 양도차익이 발생하지 않아서 분할방식의 선택을 통한 절세효과를 얻을 수 없었던 것을 확인할 수 있다. 이와 같이 조세비용의 측면에서는 전혀 유인이 없는 상황에서 동부그룹이 단기간에 4건의 물적분할을 한 것은 조세비용에 비해 비조세비용이 분할의사결정에 더욱 중요했던 것으로 추정할 수 있다. 즉, 이들 물적분할에 대하여 실무계에서는 악화된 그룹의 재무 구조를 개선하기 위한 것이라는 분석이 제기되었고, 실제로 물적분할을 통해 취득한 분할신설법인의 주식을 단기간에 매각한 사실도 확인할 수 있으므로 이러한 추론이 타당하다고 할 수 있을 것이다. 따라서, 본 연구의 분석을 통해 물적분할의 과정에서 분할법인과 분할신설법인의 세금부담을 동시에 고려하는 모든 거래당사자들(all parties)의 관점과 함께 조세비용과 비조세비용을 동시에 고려하는 모든 비용(all costs)의 관점도 중요하게 고려되어야 한다는 점을 확인할 수 있다. 또한, 이들은 효과적인 세무계획(effective tax planning)을 수립하기 위해 반드시 고려해야 하는 관점들이기 때문에 기업들이 중요한 의사결정에서 효과적인 세무계획을 실행하기 위해 다양한 측면에서 사전적으로 검토하는 사실도 간접적으로 확인할 수 있다.

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조선시대 한양 동부(東部) 함춘원(含春苑)에 관한 연구 (A Study on Dongbu Hamchunwon in Hanyang during the Joseon Dynasty)

  • 오준영;김영모
    • 헤리티지:역사와 과학
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    • 제50권3호
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    • pp.110-125
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    • 2017
  • 이 연구는 한양 도성의 동부(東部)에 조성되었던 왕실 원유(苑?) 함춘원(含春苑)을 대상으로 조영 사실과 운용적 특징의 규명에 목적을 두고 있다. 연구결과는 다음과 같이 요약될 수 있다. 성종(成宗) 연간에 창경궁이 건립된 이후 대비(大妃)들의 이어(移御)가 예정되면서 통망처(通望處)를 중심으로 논의된 차폐용 수목 식재가 동부 함춘원의 시원적 형태로 추정된다. 동부 함춘원은 창경궁 동측 궁장(宮墻)에 부속된 월근문(月覲門)부터 선인문(宣仁門)에 이르는 구간의 건너편 일대에 조영된 종방향의 원유(苑?)이었으며, 오늘날 서울대학교 병원 신축 본관의 서측 일대에 해당한다. 특히 조선시대에는 궁궐 내부에 조성된 원유만이 금원(禁苑)으로 불렸지만, 궁궐 외부에 조영되었던 동부 함춘원도 금원에 비견되는 상징적 위계적 공간으로 인식되었다. 동부 함춘원의 입지처(立地處)는 한양의 풍수지리 체계에서 내청룡(內靑龍)에 해당하는 요지(要地)였으며, 내청룡의 지맥(地脈) 보호를 위해 대규모의 토목공사는 허용되지 않았다. 인조(仁祖) 연간에 이르러 동부 함춘원의 일부는 마장(馬場)으로 분할되었다. 마장 일대는 마동산(馬東山), 방마원(放馬苑)으로도 불리었고, 휴식과 유락(遊樂)의 장소로 이용된 개방적 성격의 공간이었다. 동부 함춘원의 조성 초기에는 창경궁으로의 조망을 차폐하기 위해 속성수(速成樹)가 식재되었지만, 경모궁(景慕宮) 건립 이후에는 내청룡의 지기(地氣)를 보완하기 위해 대대적인 식목(植木) 공사가 이루어졌다. 특히 정조 연간에 유근문(?覲門)과 유첨문(?瞻門)이 건립된 이후 동부 함춘원은 궁궐(창경궁)-원유(함춘원)-사묘(경모궁)로 이어지는 전배(展拜) 동선의 거점으로 기능하였다.