• Journal of Applied Biological Chemistry
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• 제66권
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• pp.186-196
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• 2023

Dyglomera^® is an aqueous ethanol extract derived from the fruit and pods of Dichrostachys glomerata. A previous study has revealed that Dyglomera regulates adipogenesis and lipolysis by modulating AMP-activated protein kinase (AMPK) phosphorylation and increased expression levels of lipolysis-related proteins in white adipose tissue of high fat diet-induced mice and 3T3-L1 adipocyte cells. To further investigate mechanisms of Dyglomera, additional studies were performed using 3T3-L1 cells. Results revealed that Dyglomera downregulated adipogenesis by inhibiting the protein kinase B/mammalian target of rapamycin signaling pathway and reconfirmed that it downregulated gene expression levels of proliferator-activated receptor (PPAR)-γ, CCAAT enhancer binding protein α, sterol-regulation element-binding protein-1c. Dyglomera also reduced adipokines such as tumor necrosis factor alpha, interleukin-1β, and interleukin 6 by regulating leptin expression. Moreover, Dyglomera promoted beige-and-brown adipocyte-related phenotypes and regulated metabolism by increasing mitochondrial number and expression levels of genes such as T-box protein 1, transmembrane protein 26, PR domain 16, and cluster of differentiation 40 as well as thermogenic factors such as uncoupling protein 1, proliferator-activated receptor-gamma co-activator-1α, Sirtuin 1, and PPARα through AMPK activation. Thus, Dyglomera not only can inhibit adipogenesis, but also can promote lipolysis and thermogenesis and regulate metabolism by affecting adipokine secretion from 3T3-L1 adipocytes.

• The Plant Pathology Journal
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• 제40권2호
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• pp.115-124
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• 2024

Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.

• 방사선산업학회지
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• 제17권4호
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• pp.533-542
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• 2023

We developed an emergency national manual for preservation of cultural heritage using irradiation disinfection technic under flood disaster. And we examined its practicality with a critical radiation dose on fungi that occur at water-logged event in order to prevent fungal damage that occurs during submersion. The X-ray irradiation for this experiment was conducted at the Production Technology Research Institute located in Yeongcheon, Gyeongsangbuk-do. A disinfection critical dose of 12 kGy was selected for two types of fungi known to spread rapidly and are resistant to radiation to submerged cultural properties, and this experiments were conducted by setting a target dose of 12kGy at 8.37mA at 5MeV. Under the above conditions, only continuity of irradiated samples were completely disinfected. This suggests that continuity of irradiation is important for fungal disinfection.

• The Plant Pathology Journal
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• 제40권4호
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• pp.408-413
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• 2024

The emergence of rice black-streaked dwarf virus (RBSDV) poses a significant threat to global cereal crop cultivation, necessitating the urgent development of reliable detection and quantification techniques. This study introduces a reliable approach for the precise and sensitive quantification of the RBSDV in cereal crop samples, employing a reverse transcription digital polymerase chain reaction (RT-dPCR) assay. We assessed the specificity and sensitivity of the RT-dPCR assay proposed for precise RBSDV detection and quantification. Our findings demonstrate that RT-dPCR was specific for detection of RBSDV, with no cross-reactivity observed with other viruses infecting cereal crops. The RT-dPCR sensitivity was over 10 times that of RT-quantitative PCR (RT-qPCR). The detection limit of RT-dPCR was 0.096 copies/㎕. In addition, evaluation of RT-dPCR assay with field samples was conducted on 60 different cereal crop samples revealed that RT-dPCR (45/60) exhibited superior accuracy compared with RT-qPCR (23/60). In this study, we present a specific and accurate RT-dPCR assay for the detection and quantification of RBSDV.

• 한국지리정보학회지
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• 제7권3호
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• pp.57-69
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• 2004

본 연구는 낙동강 하구 일대의 생태계 변화와 생태 관련자료를 GIS DB로 구축하고 생태지도를 작성하였다. 생태지도 작성을 위해 1984년 11월 21일, 1997년 5월 17일에 촬영된 공간해상도 30m의 Landsat TM 위성영상과 국립지리원에서 발행된 1:25,000 수치지형도, 부산시에서 조사한 생물현황 자료를 바탕으로 DB를 구축하였다. 생태지도를 작성하기 위해, 첫째, 낙동강 하구 생태계 조사 보고서와 현재까지 진행된 생태지도에 대한 문헌 연구, 둘째, 시계열적 토지피복분류도 제작, 셋째, 동 식물상, 수질 등 생태계 항목별 DB 구축과 3단계 방식에 의한 생태계 평가, 최종적으로는 이러한 분석 결과를 바탕으로 생태계 관리를 위한 생태지도를 작성하였다.

• 대한한방내과학회지
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• 제16권1호
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• pp.17-32
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• 1995

The purpose of this is to understand the concept of Sacho on the basis of 'Sa-Sang philosophy(四象哲學)'. In oder to achieve this purpose, Sanjiao and Sacho were investigated, centering on the philophicalbasis, region. action. The conclusion would be summerized as follows. 1. Viewed in the light of a philosophical basis, Sanjiao is first recorded in Nae-Kyung(內經), a concept that Yin-Yang(陰陽), Oh-Hang(五行) in Nae-Kyung Medcine, the cosmos's order and it's moving rule could be directly applied to human body. On the contrary Sacho is a term first used by Lee Jae-Ma, a human-oriented concept that formed in Confucianism system. based on Sa-Sang type classifying in Sa-Sang Medicine. 2. Viewd in the light of a region, the upper part of Sacho is similar to the upper-jiao of Sanjiao, the mid-upper part of Sacha to the middle-jiao of Sanjiao, the mid-lower and the lower parts of Sacha to the lower-jiao of Sanjiao. But these regions are not equal to anatomical regions. Sanjiao and Sacha is similar concept from the view point of the upper and lower oder, but Sanjiao has only a concept of the upper and lower order, doesn't have a concept of the front and the rear, inside and outside. 3. Viewed in the light of a action, Sanjiao and Sacho is a similar concept from the view point of a large boundary in the life activity.Sanjiao explains the region of human body, setting imfortance on the viscera physiology, on the contrary Sacho explains the whole function of human body including one's heart's desire, physical constitution and the visceral physiolosy. 4. Viewed in the light of produsing-wasting activity. sanjiao and Sacho have both produsing action and wasting action. but Sacho has a more concrete concept of produsing action by using a term-Sa Hae(四海) 5. Viewed in the light of vital energy(氣)'s action, Sajiao give the first consideration to the rising and falling action of vital energy, on the contrary Sacho give the first consideration to the transversal circulation of vital energy and the deviation of it in each part. According to the above results, Sacho must be discussed separately from Sanjiao, it is recommended that further study is necessary to understand the concept of Sacho on the basis of Sa-Sang philosophy.

• 대한물리의학회지
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• 제10권4호
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• pp.107-112
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• 2015

PURPOSE: This study investigated the effects of lower limb and trunk muscles activation on seat angle and height during sit to stand and stand to sit. METHODS: Subjects were instructed to sit and stand on different angles and heights; $0^{\circ}$(43cm), $5^{\circ}$(48cm), $10^{\circ}$(51cm) and $15^{\circ}$(58cm). Measurements were conducted from sit to stand and stand to sit for external oblique, transversus abdominis, rectus femoris, vastus medialis, vastus lateralis, tibialis anterior and gastrocnemius muscle activation by surface EMG. The muscle activation was measured three times for five seconds in each posture, and the %RVC value was calculated after the exclusion of one second before and after this measurement. This calculation standardized the data from each participant so it could be comparatively analyzed. RESULTS: The external oblique, tibialis anterior, and erector spinae muscles showed significant difference in $10^{\circ}$(51cm), $15^{\circ}$(58cm) and $0^{\circ}$(43cm), rectus femoris, in $15^{\circ}$(58cm) and $0^{\circ}$(43cm)(p<.05) during sit to stand. The external oblique, tibialis anterior, and rectus femoris muscles showed significant difference in $0^{\circ}$(43cm) and $15^{\circ}$(58cm), erector spinae and vastus medialis muscles showed significant difference in $10^{\circ}$(51cm), $15^{\circ}$(58cm) and $0^{\circ}$(43cm)(P<.05) during stand to sit. CONCLUSION: The present study findings indicate that the toilet seat angle and height could affect muscle activities of the lower limb and trunk.

• 열처리공학회지
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• 제20권2호
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• pp.84-93
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• 2007

Microstructural changes during tempering at the temperature range of $300^{\circ}C{\sim}700^{\circ}C$ for the nitrogen-permeated STS 410 and 410L martensitic stainless steels has been investigated. After nitrogen permeation at temperature between 1050 and $1150^{\circ}C$, the surface layer appeared fine $Cr_2N$ of square and rod types in the martensite matrices. Hardness of the nitrogen-permeated surface layer represented 680Hv and 625Hv, respectively, for 410 and 410L steels. It is considered that the fine homogeneously dispersive effect of precipitates by nitrogen caused the increased hardness. Due to the counter current effect of carbon from interior to surface during nitrogen diffusion from surface to interior, the 0.1%C alloyed 410 steel showed the low nitrogen content of 0.025% compared with 0.045% of 410L steel at the distance of $100{\mu}m$ from the surface. Tempering of nitrogen-alloyed 410 and 410L showed the maximum hardness at $450^{\circ}C$. This maximum hardness was considered to be the secondary hardening effect of very fine carbide and nitride. The decrease in hardness at $700^{\circ}C$ was the softening effect of the matrix due to the precipitation of many needle-shaped $Cr_2N$ for 410 steel and the precipitation of coarse nitride of $Cr_2N$ in line with the spherical precipitates with directionality for 410L steel. For 410 steel, the corrosion resistance of nitrogen permeated surface in the solution of 1 N $H_2SO_4$ were nearly unchanged, however the superior corrosion resistance was obtained for nitrogen permeated 410L steel compared to the solution annealed condition.

• 생명과학회지
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• 제15권3호
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• pp.505-512
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• 2005

신경흥분독성과 간질발작발현은 glutamate 수용체활성과 연관이 있다고 알려져 있다. a-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA), kainate 수용체에 대한 glutamate 활성을 포함하는 다양한 기전을 가진 항전간제인 Topiramate는 신경보호작용을 가진다는 증거가 제시되어 Topiramate가 간질발작 후 해마의 glutamate 수용체 발현에 미치는 효과를 관찰하였다. 흰쥐에 kainate를 복강 내 주사하여 간질중첩증을 유발시킨 후 Topiramate를 1주일 주사하였다 Apop tag in situ detection kit를 이용하여 세포손상을 관찰한 결과 kainate 유발 간질중첩증 1주일 후 해마의 CA1, CA3에서 심각한 세포사를 보였으나, Topiramte 처리 군에서는 세포사가 현저히 감소하였다. 간질중첩증 이후 NMDA 수용체 아형 1,2a, 2b 발현이 현저히 증가했으나 Topiramate 처치에 의해 NMDA수용체의 발현에는 뚜렷한 변화가 없었다. AMPA수용체에서는 GluR1이 간질중첩증 이후 현저히 상향 조정되었고 GluR2는 현저히 하향조정 되었다 Topiramate 1주일 처리 시 간질중첩증으로 인해 변화된 CluR1과 GluR2의 발현이 역전되었다. 결론적으로 Topiramate는 간질중침증에 의한 CluR1/CluR2 발현비의 증가로 유발되는 흥분성 신경세포사를 억제시킴으로써 신경보호작용이 있는 것으로 보인다.

• 한국결정성장학회지
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• 제26권3호
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• pp.89-94
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• 2016

본 연구에서는 HVPE(Hydride Vapor Phase Epitaxy)를 이용하여 각각 다른 V/III ratio를 가지는 multi-step의 성장 시간 변화에 따라 r-plane 사파이어 위에 성장되는 a-plane GaN 에피층의 결정성에 대하여 연구하였다. 또한 이번 연구의 결과를 선행 연구에서 single-step으로 r-plane 사파이어 위에 성장시킨 a-GaN 에피층의 결과와 비교하였다. Multi-step으로 r-plane 사파이어 위에 a-plane GaN 에피층을 성장시켰을 때, source HCl의 유량과 성장 시간이 증가함에 따라 a-plane GaN 에피층에 대한 rocking curve의 FWHM(Full Width at Half Maximum) 값이 감소하였다. 높은 source HCl의 유량을 갖는 first step과 second step의 성장 시간과 source HCl의 유량이 증가할수록 a-plane GaN 에피층 내부의 void가 감소하였다. 결과적으로 first step과 second step의 성장 시간이 가장 긴 조건에서 성장된 a-plane GaN 에피층이 가장 낮은 FWHM 값인 584 arcsec을 가지며, azimuth angle의 의존도가 가장 적은 것으로 확인되었다.