• Journal of Animal Science and Technology
• /
• 제52권2호
• /
• pp.91-96
• /
• 2010

본 연구는 돼지고기 원산지 식별에 활용될 수 있는 최적의 SNP marker set을 개발 및 확립하기 위해 수행되었다. 선발된 51개의 SNP marker들의 효율성, 다형성 및 독립성 검증을 실시하였으며 51개의 SNP marker set은 MassARRAY method에 의해서 Multiplex-PCR panel 4개로 디자인 되었으며, 농장별, 생산조합별, 모돈별, 웅돈별로 효과적으로 고유 유전자형 지문분석이 가능하게 제작되었고, 다른형태의 SNP 유전자형 분석 플랫폼에 적용될 수 있는 적절한 마커 갯수이다. 또한 51개의 SNP marker set을 적용하여 모의 실험 및 친자감별확율을 계산하였을 때 무작위 교배 집단(PI), 반형매 교배집단($PI_{half-sib}$)과 전형매 교배집단($PI_{sibs}$)을 통해 모의 실험을 한 결과 $5.63{\times}10^{-33}$, $4.35{\times}10^{-15}$ 그리고 $1.32{\times}10^{-15}$로 분석되었으며 친자확인률에서도 모두 100%에 가까운 확률값을 나타내었다. 따라서 본 연구에서 개발된 SNP marker set을 이용하여 돈육제품의 원산지를 추적에 이용한다면 개별돼지의 고유한 DNA 지문 정보를 생성할 뿐만 아니라, 이를 통하여 모돈과 웅돈을 식별하여 농장원산지를 확인이 가능 할 수 있을 것으로 사료된다. 따라서 국내산 돼지의 생산에서부터 돈육제품으로의 소비까지 이력추적이 가능한 도구로 제공 될 것이다.

• 대한수의학회지
• /
• 제35권2호
• /
• pp.405-416
• /
• 1995

말의 뇌화연증(equine leukoencephalomalacia)과 돼지의 폐수종(porcine pulmonary edema)은 Fusarium에 오염된 옥수수로 인하여 발생되는 것으로 추정되어 왔다. 1988년에 F moniliforme에서 2차 대사산물인 fumonisin $B_1(FB_1)$이 동정되면서 오염된 옥수수와 순수 분리된 $FB_1$으로 두질병이 실험적으로 재현되었고, 말과 돼지 이외의 다른 가축에 대해서도 독성 연구가 진행되고 있다. fumonisins(FBs)는 모든 종에서 간에 독성을 나타내나 종에 따라 주요 독성 장기가 각기 다름이 밝혀지고 있다. FB의 독성 기전에 대해서는 잘 알려지지 않았으나 FB가 sphingolipid 생성과정을 차단함으로써 장기 및 혈중에 sphinganine(SA) : sphingosine(SO)를 증가시키는 것으로 알려졌다. 이는 증가된 SA : SO가 FB 독성의 진단기준이 될 수 있음을 시사하는 것이다. 최근 진행 중인 연구에 의하면, 저용량의 $FB_1$ 급식 투여가 돼지에서 혈중 입자(blood-born particle)에 대한 폐혈관 대식 세포(pulmonary intravascular macrophage)의 탐식 능력을 저하시켜, 세균 감염에 대한 감수성이 증가될 수 있음을 시사하고 있다. Fusarium 속균은 전세계적으로 생산되는 옥수수에서 발생되고 있으며, 우리나라는 사료에 사용되는 옥수수의 절대량을 수입에 의존하고 있는 점을 고려할 때, 허용기준 및 무해용량 등에 대한 관리가 절실하다. 이 논문에서는 최근 연구된 FB에 의한 가축 독성에 대하여 기술하고자 한다.

• 한국동물위생학회지
• /
• 제43권1호
• /
• pp.23-29
• /
• 2020

Hepatitis E Virus (HEV) infection is a worldwide disease and the primary cause of acute viral hepatitis in the world. It can be isolated from many different species including pigs. HEV is a zoonotic pathogen and foodborne disease. The main animal reservoir is domestic pigs. It is usually asymptomatic in pig but it is a public health concern, causing acute hepatitis in humans of varying severity. This study focused on the presence of HEV in pig and pork product. One hundred feces and one hundred fifty serum samples were randomly collected from pigs in slaughterhouses in Gwangju from November in 2018 to February in 2020. In addtion, seventy-five pork products were collected from markets in Gwangju. Feces and pork product samples were examined for the presence of HEV RNA using an reverse-transcription realtime PCR (RT-qPCR) assay. Serum samples were tested for the presence of HEV-specific IgG antibodies using Enzyme-linked immunosorbent assay (ELISA). HEV antigen and antibody positive rates were 3.0% (3/100) and 19.3% (29/150), respectively, in Gwangju and nearby areas such as Jeonnam and Jeonbuk. However, HEV antigen was not detected from any of pork product in this study. In conclusion, the prevalence of HEV should be continuously monitored because HEV was sporadically detected in Gwangju and nearby areas.

• 한국농공학회지
• /
• 제16권2호
• /
• pp.3420-3429
• /
• 1974

This study was performed to estimate the daily water consumption for watering livestock and home use, and to organize the water systems in the rural districts of Korea. For these purposes, 560 farms and other 40 urban families were randomly chosen and investigated about their water systems and daily water consumption seasonally from July 20 to August 31, 1973, and other 82 livestock raisers' useful data for watering livestock helped the study to assess the consumption of livestock water and compare it to that of general farms which bred some domestic animals as their sidelines. The results of data showed that the daily consumption of water was varied with the difference of districts and seasons in which the investigations were conducted. The results were summarized as follows. 1. The mean consumption of water for home use was varied with the range of 30.2-48.7 lit./day in summer and 22.4-45.2 lit./day per a person, respectively, in the rural districts, which showed that the water consumption in summer was more than in autumn. 2. The mean consumption of livestock water in the general farms approached to about 15.8-37.1 lit./day per cattle, 3.87-16.45 lit./day per a pig, and 0.19-1.03 lit./day per chicken, but it was slightly reduced in autumn; 10.89-37.09 lit./day per cattle, 3.87-12.63 lit./day per a pig, and 0.06-0.94 lit./day per chicken. The ratio of livestock water consumption to home use amounted to the range of 10-25%. 3. The mean consumption of livestock water used at the livestock raisers amounted to 134 lit./day per dairy cow, 67.4 lit./day per cattle, 43.29 lit./day per a horse, 13.24 lit./day per a pig, 0.438 lit./day per a layer, and 0.177 lit./day per a broiler, which showed that the daily water consumption for larger livestock was about two times as much as the farms, but it was approximately of the same for small ones. 4. The factors affecting the amount of water consumption for home use were statistically analyzed to find the kind of water sources and the class of water systems by the factorial arrangement method to the fundamental experimental unit, Suwon area of 100 of families, in which the former was very significant while the latter was significant. However, the standard of living and their interactions were not significant. 5. Almost 83.24% of the sampled farms used some kinds of wells as their water sources, of which 32.06% was of hard water, and of which 11.l% or 2.6% contained much of iron or organice materials, respectively, examining them by a simple technique of sensibility, 6. The resulting chart of the farm water systems was summarized as Fig. IV-6. Observating the safe distance from contamination sources, the ratio of 42.4% of sampled farms whose water sources were wells, confronted highly to the danger of contamination to their water sources. And other dangerous sources of contamination such as toilets, stalls, and compost heaps were situated nearly to the water sources, and most of them were able to make their spoiled water infiltrated into the ground. Thereafter, it was recommended to develop some protecting methods and to install some suitable faculties to clean water.

• 대한수의학회지
• /
• 제39권3호
• /
• pp.489-500
• /
• 1999

The fatty acid composition of muscle were investigated to compare muscle composition among the 9 domestic animals including cattle. In major domestic animals, analyzed the effects of age, part and sex of the animal on their fatty acid composition. The content of 4 types of major fatty acids of muscle was determined and calculated their ratio. Myristic acid and palmitic acid levels were high in chicken and sheep. Besides dog muscle contained a lot of stearic acid. Linoleic acid content showed evident difference in the content depending on the animal species. The ratios of linoleic acid/palmitic acid (L/P ratio) and linoleic acid/stearic acid(L/S ratio) were characteristically high in horse and pig, whereas the ratio of palmitic acid/stearic acid(P/S ratio) was $0.71{\pm}0.17$, showing very low level in dog. As for the content of stearic acid, in cattle and chicken it was higher in young animal than adults. In duck, the contents of all fatty acids and ratio were increased by the age. As for the content of fatty acids according to the part of chicken, high level was shown in thigh than in breast and wing, while there was no remarkable variation by the part in other animal. The differences in the content of myristic acid, palmitic acid and linoleic acid among some animal could be verified in muscle lipid composition. The L/P ratio which maintained certain level regardless of age, part, sex shown distinctive pattern between the species.

• 대한수의학회지
• /
• 제39권3호
• /
• pp.478-488
• /
• 1999

The contents of histidine dipeptides, a metabolic products of muscle protein, were investigated to compare muscle composition among the 9 domestic animals including cattle. In major domestic animals, analyzed the effects of age, part and sex of the animals on their muscle composition. Large amounts of carnosine($68.63{\pm}17.41{\mu}mol/g$) were detected in cattle and it was higher than other animals. Whereas the content of anserine showed high level in order of turkey, chickens and duck. The ratio of carnosine and anserine(C/A ratio) was different depending on the animal species. Statistical data indicated that difference among species was significant(p < 0.05). The contents of histidine dipeptides in heated muscle by boiling for 40 minutes at $110^{\circ}C$ was similar to thoes of raw muscle. C/A ratio in heated muscle was not different from that of raw muscle, indicating that no change has been made after heating process. The contents of carnosine and anserine were different according to the parts of their muscle. Especially chuck of the mammalian showed extremely low level of histidine dipeptides compared with other parts, while C/A ratio maintained certain level regardless of age, part, sex. Therefore, based on the content of histidine dipeptides, could be found the difference of muscle composition among the species. Also C/A ratio of horse, pig, cattle, duck, sheep and turkey were characteristic respectively.

• 한국동물학회지
• /
• 제13권2호
• /
• pp.51-56
• /
• 1970

Studies on the feeding and resting behavior of Culex pipiens pallens, a possible vector of Japanese encephalitis in Korea, was carried out at Kobu-Ri, Kobu-Myon, Chongup Kun. Cholla Pukdo in 1967. 1. Indoor and outdoor human biting collections showed that 47.3 per cent Culex pipiens pallens were collected indoors and 52.7 per cent outdoors. 2.Feeding occurred throughout the night from dusk to dawn, with the peak period from 23:00-24:00 hours outside the house and from 24:00-01:00 hours inside. 3. Culex pipiens pallens females preferred man for their blood feeding to domestic animals. The results of human and cow biting collections showed that the feeding ratio between man and cow was 2.1 to 1. As to the feeding preference to the domestic animals, the ratio among cow, pig and horse was 7.3 to 5.1 to 5.4 4. Daytime resting collections showed that large numbers of Culex pipiens pallens females rest inside the house, especially in the bed room and the chicken pen. As temperatures increased, fewer mosquitoes resting indoors and more mosquitoes rest ing outdoors were caught. By means of sweep-net collections at outdoors resting places, a small number of Culex pipiens pallens mosquitoes were caught in such places as grass fields, vegetable fields, barley fields, bean fields, culverts, bomb shelters, and so on. A comparison between number of mosquitoes collected resting during the day in bedrooms and verandahs with resting collections in the same places at night showed that more mosquitoes were present at night. It was observed that the majority of the unfed females rested before feeding at night.

• Journal of Veterinary Science
• /
• 제22권5호
• /
• pp.71.1-71.12
• /
• 2021

Background: African swine fever (ASF) is a hemorrhagic fever occurring in wild boars (Sus scrofa) and domestic pigs. The epidemic situation of ASF in South Korean wild boars has increased the risk of ASF in domestic pig farms. Although basic reproduction number (R₀) can be applied for control policies, it is challenging to estimate the R₀ for ASF in wild boars due to surveillance bias, lack of wild boar population data, and the effect of ASF-positive wild boar carcass on disease dynamics. Objectives: This study was undertaken to estimate the R₀ of ASF in wild boars in South Korea, and subsequently analyze the spatiotemporal heterogeneity. Methods: We detected the local transmission clusters using the spatiotemporal clustering algorithm, which was modified to incorporate the effect of ASF-positive wild boar carcass. With the assumption of exponential growth, R₀ was estimated for each cluster. The temporal change of the estimates and its association with the habitat suitability of wild boar were analyzed. Results: Totally, 22 local transmission clusters were detected, showing seasonal patterns occurring in winter and spring. Mean value of R₀ of each cluster was 1.54. The estimates showed a temporal increasing trend and positive association with habitat suitability of wild boar. Conclusions: The disease dynamics among wild boars seems to have worsened over time. Thus, in areas with a high elevation and suitable for wild boars, practical methods need to be contrived to ratify the control policies for wild boars.

• Animal Bioscience
• /
• 제37권4_spc호
• /
• pp.775-785
• /
• 2024

Pork production is a significant agricultural enterprise in the United States and Canada. The United States is the third-largest global producer of pork and Canada ranks seventh in pork production. The North American Free Trade Agreement and its successor, the U.S.-Mexico-Canada Agreement, have facilitated trade and integration between the two countries. The majority of production systems are modern and intensive, characterized by large vertically integrated farms using advanced technologies. Both nations benefit from their status as major producers of feed grains, with the United States leading in corn and soybeans, while Canada excels in canola and barley production. The regulatory frameworks for food safety, animal welfare, and environmental stewardship differ slightly, with the FDA and USDA overseeing these aspects in the United States, and Health Canada and the Canada Food Inspection Agency in Canada. The United States and Canada also have well-established distribution networks for pork products, relying on both domestic and international markets. Export markets play a crucial role, with the United States being a major importer of Canadian pigs, and both countries exploring opportunities in Asia. Despite a rise in global demand, domestic pork consumption trends differ, with per capita consumption remaining stable in the USA and declining in Canada. Changing consumer preferences, including a demand for ethically raised and locally sourced pork, may influence production practices. Future trends in pig production include a focus on consumer concerns, sustainability, disease prevention, reduction of antimicrobial use, and advancements in technology. The industry is adapting to challenges such as disease outbreaks and changing regulations, with a strong emphasis on animal welfare. Labor and workforce considerations, along with advancements in technology and automation, are expected to shape the efficiency of pork production in the future.

• 한국동물위생학회지
• /
• 제24권3호
• /
• pp.243-253
• /
• 2001

Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and S enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.