• BMB Reports
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• 제43권8호
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• pp.567-572
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• 2010

In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCC-binding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3-hy15.

• 지능정보연구
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• 제10권2호
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• pp.111-131
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• 2004

소프트웨어 유지보수 단계에서는 고객니즈, 마케팅 정책, 법, 제도의 변화 등으로 인한 다양한 시스템 변경 요구를 수용하여야 한다. 그러나, 소프트웨어의 비가시성문제로 인해 새로운 변경 요구사항 발생 시 수정 대상 모듈을 발견하는데 지대한 시간이 요구될 뿐 아니라 모듈의 재 사용을 어렵게 만들어 중복 모듈이 양산 됨으로써 향후 장애의 근원이 되는 악순환이 전개된다. 이에 본 연구에서는 시맨틱 웹(Semantic Web) 기술을 활용하여 이동통신사의 과금/청구 도메인의 관리자와 개발자들이 공유하고 있는 개념과 개념간 관계를 명시적으로 표현하고 이를 이용하여 변경대상 모듈을 쉽게 발견 할 뿐 아니라, 발견된 모듈에 대해 구조적 호출 및 조립 관계를 분석하도록 지원하는 온톨로지 기반 변경 영향 분석 시스템(OntCIA; Ontology based Change Impact Analysis System)을 제시한다. OntCIA는 스트링 매칭과는 근본적으로 다른 의미적 모듈검색을 지원하며 잦은 변경이 요구되는 호출 및 조립 구조 정보는 데이터 베이스에서 관리하고 도메인 지식은 온톨로지로 관리함으로써 유지 보수가 용이한 구조를 가진다.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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• pp.244-251
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• 2003

A two-dimensional BEM code, $FRACOD^{2D}$, was applied to simulate fracture initiation and propagation processes in a rock pillar during an in situ heater test of a rock pillar planned at the $\"{A}sp\"{o}$ Underground Rock laboratory of SKB, in Southern Sweden. To take the advantage of conventional BEM for simulating fracturing processes, but without efforts for domain integral transformation, a hybrid approach is developed to simulate the fracturing processes in rock pillar under coupled thermo-mechanical loading. The code FRACOD was used for simulating the fracture initiation and propagation processes with its boundary tractions reflecting the effects of the initial and redistributed thermomechanical stresses in the domain of interest at multiple excavation and heating steps were produced by a special algorithm of stress inversion, based on resultant thermo-mechanical stress fields at each excavation and heat loading step by a FEM code without considering fracturing processes. This hybrid approach can take the advantages of both types of numerical methods and avoids their shortcomings for fracturing process simulation and domain effects, respectively. In this paper, we present the hybrid approach for the stress, displacements, and fracturing processes at sequential excavation and heating steps of the in situ heater test as a predictive modelling, the formulation of the fracturing models and the predictive results. Two sections of borehole depth, 0.5 m and 1.5 m below the tunnel floor are considered. The pillar area is modelled with the FRACOD and the stress field produced by excavation and heating is transferred with corresponding boundary stresses. From the modelling results, the degree of fracturing and damage are evaluated for 120 days of heating. Dominated shear fracturing in the vicinity of the central pillar was observed from the models at both sections, but spalled area appears to be limited. Based on the modelling results, a sensitivity study for the effect of pre-existing fractures in the vicinity of the holes is also conducted, and the initiation and evolution of EDZ around the deposition holes are investigated using this particular numerical technique.

• 대한조선학회논문집
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• 제31권2호
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• pp.45-56
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• 1994

2차원 수중익 주위의 비선형 자유표면 유동을 시간영역에서 시뮬레이션할 수 있는 수치해법이 개발되었다. 본 수치해법은 고차 스펙트럴법과 경계요소법을 조합한 스펙트럴/경계요소법(spectral/boundary-element method)이며, 자유표면은 고차 스펙트럴법에 의해 그리고 수중익과 후류 보오텍스는 경계요소법에 의해 다루어 진다. 본 방법은 자유표면과 수중익의 비선형/비정상 상호작용문제에 폭넓게 적용될 수 있으며, 특히 스펙트럴법을 사용하므로 자유표면 유동을 매우 효율적으로 다룰 수 있다. 적용예로 정지상태에서 출발하여 자유표면 근처에서 균속 전진운동 또는 전진 및 동요운동을 하는 경우가 다루어 졌고, 자유표면파와 동유체력에서의 비선형/비정상 효과들이 보여지고 있다. 비정상해의 특수한 경우로 얻어지는 정상상태의 계산결과들이 다른 이론 또는 실험결과들과 비교되었으며, 좋은 일치를 보이고 있다.

• 한국간호교육학회지
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• 제9권1호
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• pp.144-154
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• 2003

Recently, in the academic disciplines critical thinking has been emphasized as the new evolution in education. Problem-based learning is suggested as the evolutionary learning method in developing critical thinking. In the nursing education, PBL is offered as an appropriate teaching method to prepare nurse student for professional. PBL is a student-centered learning strategy aimed at developing critical thinking, motivating self-directed study and attaining autonomy. The purpose of this study was to develop the communication curriculum of nursing education based on PBL and implementing of it. PBL module was developed focused on five communication situations. And learning strategies to facilitate the learning process and the guided questions to stimulate student inquiry were also developed. This PBL education was conducted for six students in the master's course during the 14 weeks from March, 2000 to June, 2000. The outcomes of the PBL education were examined based on the content analysis of the students reflective journal. As a result, it was found that students experienced the effect of it focused on self-oriented, group-oriented, and practice-oriented domain. In the self-oriented domain, the findings indicate that there were 5 kinds of concepts including 'motivation for self-directed study', 'transition toward certainty in knowledge acquisition', 'attempt to apply their prior knowledge into new situation', 'enhancement of self-image in real situation', 'self-growth with self reflection'. In the group-oriented domain, there were 3 kinds of concepts including 'cognitive work in group', 'perception of co-responsibility in attaining learning objectives', 'socialization with group members'. In the practice-oriented domain, there were 3 kinds of concepts including 'linkage theoretical knowledge with real situation', 'attempt to apply in real situation', 'development problem solving skill in real situation'. In conclusion, PBL had a significant effect on self, group, and clinical domain. And assessing PBL outcomes is challenging because standardized instrument do not develop yet. So the findings of this study can suggest the basic data for examining the PBL outcome.

• Applied Biological Chemistry
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• 제49권4호
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• pp.293-297
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• 2006

포유류의 조직에 널리 분포되어 있으며 혈압 조절에 중요한 역할을 하는 Angiotensin-converting enzyme(ACE)은 아연을 함유하는 dipeptidase로서 angiotensin I을 가수분해하여 강력한 혈압상승제인 angiotensin II를 생성하는 효소이다. 최근에 돼지의 난소에서 ACE 활성이 측정되었으며, 돼지의 신장에서 ACE 단백질이 분리되어 그 특성이 알려졌다. 그러나 돼지의 어떠한 ACE DNA 염기서열도 아직까지 보고 된 바는 없다. 그러므로 본 연구에서 reverse transcriptase-polymerase chain reaction(RT-PCR)을 이용하여 돼지의 신장 ACE cDNA를 클로닝하고 그 염기서열을 분석하였다. ACE cDNA는 1309개의 아미노산으로 구성되어 있으며 그 분자량은 150kDa이다. 염기서열로부터 유추한 아미노산의 서열을 분석한 결과, N 말단의 33개 아미노산이 signal peptide 역할을 하는 것으로 보이며, C 말단 근처의 짧은 transmembrane 영역은 세포막에 anchor역할을 하는 것으로 보인다. 돼지 신장의 ACE에서 두 개의 매우 유사한 amino acid peptidase domain은 tandem duplication 되어 있으며, 각각의 domain은 다른 포유류의 체세포 ACE들과 마찬가지로 putative metal-binding site(His-Glu-Met-Gly-His)를 하나씩 가지고 있는 것으로 나타났다. 돼지 신장 ACE 서열과 인간, 토끼, 쥐 등과 같은 포유류의 ACE 아미노산 서열들과의 상동성 비교는 진화과정 중 두 domain이 매우 잘 보전되어 왔음을 보여주고 있다.

• 한국통신학회논문지
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• 제34권5C호
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• pp.527-539
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• 2009

본 논문에서는 3GPP LTE (3rd Generation Partnership Project Long Term Evolution) MIMO-OFDMA(multiple-input multiple-output-orthogonal frequency division multiple access) 시스템의 하향 링크 수신기를 위한 asynchronous ICI (Inter-Cell Interference) 완화 기법을 제안한다. Multi-cell 환경을 고려한 celluar OFDMA 시스템에서는 기본적으로 frequency reuse factor가 1로 설정되기 때문에 셀 경계에 위치한 UE (User Equipment)의 경우 ICI 영향을 받게 되며, 특히 각기 다른 셀 반경 및 nodeB 간의 거리 차이 등 현실적인 celluar 환경을 고려 할 경우에는 UE 간 타이밍 오류가 가중되어 수신 신호의 주파수 영역의 직교성이 파괴될 가능성이 있다. 따라서 이러한 인접 셀 간섭을 제거 및 완화하기 위하여 수신 OFDM 심볼에 대한 SCM (Spatial Covariance Matrix) 추정이 필요하다. 일반적으로 SCM 추정은 training symbol을 이용함을 가정하지만, 긴 시간 동안 간섭의 통계적 특성을 측정하는 것은 어려울 뿐만 아니라 training symbol이 고려되지 않는 LTE와 같은 MIMO-OFDMA 시스템에는 적합하지 않다. 또한 추정의 정확성을 높이기 위하여 noise reduction 방식이 적용된 추정 기법이 제시되고 있으나, 기존 time-domain low-pass type weighting 방식은 spectral leakage에 의한 추정 에러를 유발하는 단점이 있다. 따라서, 본 논문에서는 noise reduction 효과를 얻으면서 spectral leakage에 의한 SCM 추정 오류를 최소화할 수 있으며, 주파수 영역에의 moving average filter로 구현 가능한 time-domain sinc-type weighting 방식의 SCM 추정 기법을 제안하였으며, 다양한 환경에서의 컴퓨터 모의 실험을 통하여 제안된 방식이 기존의 방식보다 약 3dB 의 SIR (Signal to Interference Ratio) 이득을 보임을 입증하였다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 추계학술대회 논문집
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• pp.62-65
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• 2008

Extrusion characteristics of Mg alloys were studied experimentally. The Al-Zn-Mg alloys, AZ31, AZ6l, AZ80, and AZ91 were extruded with hot hydrostatic extrusion process. The hydrostatic process was efficient to reduce surface friction and extend steady state region in extrusion which made it more convenient to examine deformation behavior of the alloys avoiding the disturbance caused by temporary contact state between billet and die, and billet and container. High pressure was cooperative to expand forming limit of the alloys which were applied on the billet during the extrusion process. Extrusion limits were traced in temperature and extrusion speed domain with changing composition of the alloying elements. Effects of process parameters on extrusion load and microstructure evolution were investigated also.

• 한국세라믹학회지
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• 제50권1호
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• pp.82-86
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• 2013

A poled lead zirconate titanate (PZT) cube specimen is subjected to impulse-type compressive stress with increasing magnitude in parallel to the poling direction at four room and high temperatures. During the ferroelastic domain switching induced by the compressive stress, electric displacement in the poling direction and longitudinal and transverse strains are measured. Using the measured responses, linear material properties, namely, the piezoelectric and elastic compliance coefficients, are evaluated by a graphical method, and the effects of stress and temperature are analyzed. Finally, the dependency of the evaluated linear material properties on relative remnant polarization is analyzed and discussed.