• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1306-1318
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• 2009

The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes. Two $\alpha$-amylases ScAmy54 and ScAmy43 predicted to play an important role in starch degradation were showed to produce specific oligosaccharides essentially maltotriose that have a considerable commercial interest. Primary structure of the two enzymes was established by N-terminal sequencing, MALDI-TOF masse spectrometry and cDNA cloning. The two proteins have the same N-terminal catalytic domain and ScAmy43 derived from ScAmy54 by truncation of 96 amino acids at the carboxyl-terminal region. Data of genomic analysis suggested that the two enzymes originated from the same $\alpha$-amylase gene and that truncation of ScAmy54 to ScAmy43 occurred probably during S. sclerotiorum cultivation. The structural gene of Scamy54 consisted of 9 exons and 8 introns, containing a single 1,500-bp open reading frame encoding 499 amino acids including a signal peptide of 21 residues. ScAmy54 exhibited high amino acid homology with other liquefying fungal $\alpha$-amylases essentially in the four conserved regions and in the putative catalytic triad. A 3D structure model of ScAmy54 and ScAmy43 was built using the 3-D structure of 2guy from A. niger as template. ScAmy54 is composed by three domains A, B, and C, including the well-known $(\beta/\alpha)_8$ barrel motif in domain A, have a typical structure of $\alpha$-amylase family, whereas ScAmy43 contained only tow domains A and B is the first fungal $\alpha$-amylase described until now with the smallest catalytic domain.

• Wind and Structures
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• 제17권1호
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• pp.87-105
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• 2013

Modeling an equilibrium atmospheric boundary layer (ABL) in an empty computational domain has routinely been performed with the k-${\varepsilon}$ turbulence model. However, the research objects of structural wind engineering are bluff bodies, and the SST k-${\omega}$ turbulence model is more widely used in the numerical simulation of flow around bluff bodies than the k-${\varepsilon}$ turbulence model. Therefore, to simulate an equilibrium ABL based on the SST k-${\omega}$ turbulence model, the inlet profiles of the mean wind speed U, turbulence kinetic energy k, and specific dissipation rate ${\omega}$ are proposed, and the source terms for the U, k and ${\omega}$ are derived by satisfying their corresponding transport equations. Based on the proposed inlet profiles, numerical comparative studies with and without considering the source terms are carried out in an empty computational domain, and an actual numerical simulation with a trapezoidal hill is further conducted. It shows that when the source terms are considered, the profiles of U, k and ${\omega}$ are all maintained well along the empty computational domain and the accuracy of the actual numerical simulation is greatly improved. The present study could provide a new methodology for modeling the equilibrium ABL problem and for further CFD simulations with practical value.

• 생명과학회지
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• 제19권11호
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• pp.1522-1528
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• 2009

알긴산을 분해하기 위하여 갈조류로부터 분해활성이 있는 해양미생물을 분리하였다. 분리된 균주의 16S ribosomal DNA를 분석한 결과 이전에 보고했던 ALG-5 균주와 비슷한 Streptomyces sp.에 속하는 것으로 나타났다. 상동성이 있는 염기서열로 고안한 특이적인 primer로 PCR을 행함로서 Streptomyces sp. M3의 새로운 alginate lyase 유전자를 클로닝하였다. M3 alginate lyase의 예상 아미노산 서열에는 N-terminal 영역에 YXRSELREM 서열과 C-terimnal 영역에 YFKAGXYXQ 서열이 보존되어 있었다. M3 alginate lyase 단백질의 homology model은 Corynebacterium sp. ALY-1으로부터 얻은 단백질인 alyPG와 같이 $\beta$-jelly roll fold를 main domain으로 가지고 있음이 나타났다. M3 alginate lyase 유전자를 가지는 재조합 E. coli의 세포균질액은 polymannuronate block보다는 polyguluronate block에 대하여 높은 분해력을 가지고 있었다. 아미노산 서열 다중정열 및 homology modeling으로부터 얻은 결과는 M3 alginate lyase가 Family PL-7으로 분류될 수 있음을 말해 준다.

• Asia pacific journal of information systems
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• 제8권2호
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• pp.53-67
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• 1998

The explosive growth of the Web is providing end-users access to ever-increasing volumes of information. The resources of legacy systems and relational databases have also been made available to the Web browser, which has become an essential business tool. Recently, model management on the Internet/Web is also proposed with its conceptual design or prototypical system like DecisionNet and DSS Web. However, they are also suffering from the same symptoms as the Web, Although we can identify the elements of a page with HTML tags and (declare) the relationships among the various document elements, they are semantically opaque to computer systems and have no domain-specific meaning. However, HTML is not extensible, so developers are forced to invent convoluted, non-standard solutions for embedding and parsing data. Extensible Markup Language (XML) is a simplified subset of SGML that has many benefits for folks who want to improve structure, maintainability, searchability, presentation, and other aspects of their document management. This paper proposes a structured markup language for model representation and management on the Web as an XML application. The language is based on a conceptual modeling framework, Object-Oriented Structured Modeling (OOSM), which is an extension of the structured modeling.

• 한국정보과학회논문지:소프트웨어및응용
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• 제36권3호
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• pp.200-207
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• 2009

인구의 급속한 고령화와 더불어 국민의 복지수준이 향상됨에 따라 건강문제에 대한 효율적인 해결방안으로 병원을 방문하지 않고 의료 서비스를 받는 U-Healthcare의 필요성이 증대되고 있다. 최근 다양한 센서들을 이용하여 사용자의 생체정보 및 주변의 상황정보들을 수집하고 분석해 사용자에게 적절한 서비스를 제공하기 위한 시스템에 대한 연구가 활발히 진행되고 있다. 기존의 U-Healthcare 서비스를 위한 시스템들은 특정 서비스만을 대상으로 하여 다른 서비스로의 확장 및 연계가 어렵고, 다수의 사용자를 대상으로 일반화된 서비스를 제공하고 있어 사용자 개개인의 특성을 고려한 서비스를 제공 기능이 미약하다. 본 논문에서는 이러한 문제점을 해결하기 위해 멀티에이전트 기반의 상황인식 에이전트를 이용해 시스템의 확장성을 높이고, GUI 기반의 모델링 툴킷 JaUCE를 사용하여 고객 개개인의 상황에 맞는 상황 정보를 모델링 함으로써 고객들에게 가장 적합한 상황인식 서비스를 제공한다. 본 논문에서는 제안된 상황 인식 에이전트와 모델링 툴킷의 이용하여 상황인식 기반의 U-Silvercare 서비스를 설계하고 구현하였다.

• International Journal of Aeronautical and Space Sciences
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• 제6권1호
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• pp.52-60
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• 2005

The CFD coupled aeroelastic analyses have significant advantages over linear panel methods in their accuracy and usefulness for the simulation of actual aeroelastic motion after specific initial disturbance. However, in spite of their advantages, a heavy computation time is required. In this paper, a method is discussed to save a computational cost in the time domain aeroelastic analysis based on the system identification technique. The coefficients of system identification model are fit to the computed time response obtained from a previously developed aeroelastic analysis code. Because the non-dimensionalized data is only used to construct the model structure, the resulting model of the unsteady CFD solution is independent of dynamic pressure and this independency makes it possible to find the flutter dynamic pressure without the unsteady aerodynamic computation. To confirm the accuracy of the system identification methodology, the system model responses are compared with those of the CFD coupled aeroelastic analysis at the same dynamic pressure.

• Journal of Information Technology Applications and Management
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• 제30권2호
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• pp.1-18
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• 2023

Recently, as technology development has accelerated and product life cycles have been shortened, it is necessary to derive key product features from customers in the R&D planning and evaluation stage. More companies want differentiated competitiveness by providing consumer-tailored products based on big data and artificial intelligence technology. To achieve this, the need to correctly grasp the required quality, which is a requirement of consumers, is increasing. However, the existing methods are centered on suppliers or domain experts, so there is a gap from the actual perspective of consumers. In other words, product attributes were defined by suppliers or field experts, but this may not consider consumers' actual perspective. Accordingly, the demand for deriving the product's main attributes through reviews containing consumers' perspectives has recently increased. Therefore, we propose a review data analysis-based required quality methodology containing customer requirements. Specifically, a pre-training language model with a good understanding of Korean reviews was established, consumer intent was correctly identified, and key contents were extracted from the review through a combination of KeyBERT and topic modeling to derive the required quality for each product. RevBERT, a Korean review domain-specific pre-training language model, was established through further pre-training. By comparing the existing pre-training language model KcBERT, we confirmed that RevBERT had a deeper understanding of customer reviews. In addition, all processes other than that of selecting the required quality were linked to the automation process, resulting in the automation of deriving the required quality based on data.

• 대한간호학회지
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• 제42권3호
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• pp.311-321
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• 2012

Purpose: This study was designed to construct and test a structural equation modeling on specific domain health status and the Selection Optimization Compensation (SOC) strategy affecting successful aging in elderly people. Methods: The model construction was based on the SOC model by Baltes and Baltes. Interviews were done with 201 elderly people aged 65 or older. Interview contents included demographics, functional health status, emotional health status, social health status, SOC strategies, and successful aging. Data were analyzed using SPSS 15.0 and AMOS 7.0. Results: Model fit indices for the modified model were GFI=.93, CFI=.94, and RMSEA=.07. Three out of 7 paths were found to have a significant effect on successful aging in this final model. Functional health status had a direct and positive effect on successful aging. Emotional health status influenced successful aging through SOC strategies. Conclusion: This study suggests that interventions for improving functional health status and for strengthening SOC strategies are critical for successful aging. Continuous development of a variety of successful aging programs using SOC strategy is suggested.

• 대한조선학회논문집
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• 제31권3호
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• pp.19-30
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• 1994

제품정보의 모델링은 엔지니어링 환경에서, 특히 통합하된 생산시스템의 핵심인 CAD/CAM 시스템의 개발을 위해 매우 중요한 과제가 되어가고 있다. 모델이란 실세계의 개념과 현상에 대한 정형적인 표현의 결과이며, 모델링이란 제품의 관련한 정보를 식별하고 추상화 하여 정형화 하는 작업이다. 본 연구에서는 선박의장시스템의 대표적인 배관시스템을 모델링하였다. 배관시스템 정보의 전주기 정보를 표현하기 위한 메카니즘은 STEP(Standard for the Exchange of Product Model Date)의 개념을 기초로 하였고, 시스템의 분석, 설계 및 구현을 위한 수단으로는 객체지향기술을 이용하였다. 본 연구에서 정의된 배관시스템 모델을 향후 제품모델개념의 배관 CAD/CAM 시스템 구현의 기초가 될 것이다.

• 한국항행학회논문지
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• 제14권1호
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• pp.108-118
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• 2010

본 논문에서는 사용자의 음악적 선호를 모델링하고, 사용자가 원하는 감정과 선호를 추론할 수 있게 도와주는 컨텍스트 기반 음악 추천 온톨로지 (COMUS)를 제안한다. COMUS는 제목, 연주자, 장르와 같은 음악의 일반적인 속성과 무드와 상황과 같이 도메인에 특화된 확장을 제공하는 확장성을 계층적인 방식으로 제공한다. COMUS는 음악 추천을 위한 도메인에 특화된 클래스들이 음악 온톨로지와 연동되도록 OWL 언어를 사용하여 개발된 음악 온톨로지이다. COMUS에 표현된 컨텍스트 정보를 사용하면, 컨텍스트 정보의 일관성을 체크할 수 있고, 규칙 기반의 추론을 통해 명시적인 정보 뿐만 아니라, 내재된 컨텍스트를 도출하는 상위 레벨의 추론이 가능하다고. 여기서 제안하는 온톨로지는 음악과 감정과 상황 사이의 복잡하고 자세한 관계를 표현할 수 있어서, 사용자가 음악 추론 어플리케이션을 위해 적절한 음악을 찾을 수 있게 해준다. 이와 관련된 음악 추천을 위한 사례 연구로써 수행한 몇 가지 실험을 보인다.