• Environmental Analysis Health and Toxicology
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• v.20 no.1
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• pp.87-95
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• 2005

Cadmium is well known as a toxic metal and has insulin mimicking effects in rat adipose tissue. This study was undertaken to investigate the effect of CdCl₂ on glucose transport and its mechanism in 3T3 - L1 adipocytes. CdCl₂ exhibits respectively 2.2 and 2.8 fold increases in the 2-deoxyglucose uptake when exposed to 10 and 25 μM of CdCl₂ for 12 hr. To investigate the stimulating mechanism of glucose transport induced by CdCl₂. Wortmannin and PD98059 were used respectively as PI3K inhibitor and MAPK inhibitor, which did not affect 2-DOG uptake. This results suggest that induced 2-deoxy-(l-3H)-D-glucose (2-DOG) uptake by CdCl₂ may not be concerned with the insulin signalling pathway. Whereas nifedipine, a calcium channel blocker inhibited the 2- DOG uptake stimulated by CdCl₂. In addition, we also measured the increased production of Reactive oxygen substances (ROS) and glutathione (GSH) level in 3T3-L1 adipocytes to investigate correlation between the glucose uptake and increased production of ROS with H2DCFDA. CdCl₂ increased production of ROS. Induced 2-DOG uptake and increased production of ROS by CdCl₂ were decreased by N-acetylcystein (NAC). And L-buthionine sulfoximine (BSO) a potent inhibitor of γ-GCS, decreased of 2-DOG uptake. Also NAC and BSO changed the cellular GSH level, but GSH/GSSG ratio remained unchanged at 10, 25 μM of CdCl₂.

• Journal of Veterinary Clinics
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• v.36 no.6
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• pp.297-300
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• 2019

Wii^® balance board (WBB) is a device that can measure and record body sway. This study was conducted to evaluate the reliability of WBB in small sized dog as inexpensive, portable and convenient tool. The center of pressure path length (CPPL) and 95% confidence ellipse area (Area 95) were evaluated with only two plates of WBB. The parameters were evaluated between no load (0 kg) and mass group (0.25-4 kg on each one plate). 23 dogs (2.3-7.3 kg) were evaluated for with hindlimb standing for 10 seconds. The mass group showed a significant value in comparison to the no load during the measurement. And intra-class correlation coefficients (ICCs) between CPPL and Area 95 revealed very high both mass and dog group. In the evaluation of medial patellar luxation (MPL) as a diagnostic tool, 80 dogs with MPL and 23 non-affected dogs were used. In studies of CPPL and Area 95, significant differences were found between non-affected and MPL groups for 10 and 30 seconds, respectively. The WBB can be used as a valid tool for evaluating hind limb standing balance and can be useful as an objective tool to present clinical results in small sized dog with MPL.

• Journal of Veterinary Clinics
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• v.38 no.6
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• pp.285-289
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• 2021

An eight-year-old, 3.4 kg, spayed female poodle dog was referred to Gyeongsang National University Animal Medical Center with obstinate bilateral retroperitoneal subcutaneous abscess. The medical history revealed ovariohysterectomy (OHE), lipoma, and mammary gland tumor excision performed two years ago. The dog presented with left retroperitoneal subcutaneous abscess discharge five months back and was treated by abscess drainage and antibiotics in a local veterinary hospital, but a relapse was reported on discontinuing antibiotics. On physical examination, the dog was febrile (40℃) and both retroperitoneal masses showed typical inflammatory reaction and purulent discharge. On serum chemistry analysis, hyperproteinemia, hyperglobulinemia, and elevated levels of C-reactive protein were observed. Cytologic examination showed degenerative neutrophils and phagocytic macrophages. Radiological examination revealed encapsulated hypoechoic subcutaneous masses in bilateral abdominal flank and the left flank mass was connected with the caudal pole of left kidney through fistula. Based on the diagnostic examination, exploratory celiotomy was recommended and left nephrectomy, and resection of bilateral masses were performed. During procedure, fistula between caudal pole of left kidney and left subcutaneous abscess was detected and suture material was identified. Resected tissue was histologically examined and diagnosed as suppurative abscess caused by the suture material. This case report describes chronic suppurative foreign body reaction including caudal pole of left kidney and bilateral retroperitoneal subcutaneous masses induced by the suture material used in OHE two years ago and the necessity of computed tomography examination to identify character of mass and extent of surgical resection.

• Korean Journal of Veterinary Research
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• v.62 no.3
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• pp.21.1-21.10
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• 2022

With the increasing population of companion dogs, the social cost derived from their behavioral problems is increasing. Therefore, it is important to understand the environment to provide experience for dogs via interactions with their owners to prevent and solve these problems. The parenting behavior of dog owners as an environmental factor has a profound impact on the behavior development of dogs, as does the parenting behavior on children. Therefore, this study aimed to develop a scale to assess the dog owner's parenting behavior. Exploratory factor analysis involving 300 participants resulted in a scale with four subscales, Positive Education, Involvement of Socialization, Intimacy, and Stable Responses, and 19 items. Confirmatory factor analysis was then performed to verify its reliability and validity. The result of parenting behaviors assessed by this scale was significantly different between a group with dogs with problematic behavior (n = 141) and those without (n = 159). Overall, a dog owner's parenting behavior involves affection and control aspects, but the role required specifically in the control aspect is distinguished from the parenting behavior with children. The findings in the present study will provide dog owners with effective education that will help prevent dogs from developing behavioral problems.

• Journal of Veterinary Clinics
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• v.29 no.6
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• pp.498-501
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• 2012

A 10-month-old intact male Maltese dog (body weight, 1.6 kg) presented with intermittent cough and abnormal heart sounds. The dog was diagnosed with a left-to-right patent ductus arteriosus (PDA) on the basis of the results of transthoracic echocardiography, color flow Doppler examination, and angiography. Transcatheter occlusion of the PDA was performed using the $Amplatz^{(R)}$ Canine Duct Occluder (ACDO) via the femoral artery. The device was placed under fluoroscopic guidance and successfully occluded the PDA. The continuous heart murmur disappeared immediately after the correction and no residual flow was detected. The dog was discharged the following day and regularly examined for complications and safety. This is the first report demonstrating the clinical application of ACDO for the transcatheter closure of PDA via the femoral artery in a small-breed dog in Korea.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.439-446
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• 2005

Ferritin is known to be the principle iron-storage protein in a wide variety of rganisms. The electro­phoretic mobility and immunological cross-reactivity of dog splenic ferritin were compared with those of horse, bovine, and pig splenic ferritin after isolation using heat treatment, salting out, column chromatography, and ultrafiltration. These isolation methods allowed the recovery of $\~84{\mu}g$ of the ferritin per g of spleen. The iron content in the dog ferritin was $22.7\%$, which appeared to be higher than those in the other mammalian ferritins tested. The electrophoretic mobility of the dog ferritin under nondenaturing conditions was similar to its bovine counterpart, whereas it was more identical to pig and horse ferritins on an SDS-polyacrylamide gel. The molecular weight of the dog ferritin subunit was 19.5 kDa on an SDS-polyacrylarnide gel, and the subunit was unable to bind with iron. The polyclonal anti-dog ferritin raised in rats was able to cross-react with the pig, bovine, and horse ferritins, upon Ouchterlony double immunodiffusiion. A Western blot analysis also revealed that the anti-dog ferritin, which specifically bound with the dog ferritin subunit, could also recognize the horse, bovine, and pig ferritin subunits and the maximum cross-reactivity was exhibited with the pig ferritin subunit, indicating that the dog ferritin is immunochemically more similar to the pig ferritin than its other mammalian counterparts. Accordingly, these results elucidate the biochemical and immunochemical characteristics of dog ferritin that might have a potential to be applied as an oral iron supplement to treat iron deficiency anemia.

• Proceedings of the KIPE Conference
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• 2011.07a
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• pp.545-546
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• 2011

본 논문에서는 디지털 방식의 부하분담 및 리던던시를 위하여 고속 CAN통신과 디지털 IO를 조합한 새로운 구조의 통신시스템을 제안한다. 기존 방식에 비해 모듈 고장을 알리는 기능과 고장난 모듈을 통신라인에서 물리적으로 분리하는 기능을 각각 담당한다. 만일 한 모듈이 고장이 발생하면 Watch-Dog1은 마스터에게 그 정보를 전달하고, Watch-Dog2는 이 모듈통신선을 물리적으로 제거한다. 이때 리던던시용 모듈이 투입되고, 마스터는 CAN통신을 통하여 어느 모듈이 고장 났는지를 체크한다. 또한 각 모듈 상태를 파악하기 위해 별도의 제어선을 사용하여 모듈의 고장 상태 및 고장난 모듈의 위치를 정확히 파악함으로서, 효율적이고 고속의 부하분담을 구현하였다. 본 논문에서는 실험을 통해 제안된 알고리즘의 타당성을 검증하였다.

• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.363-367
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• 2008

A 4-year-old female Cocker spaniel was presented with respiratory distress and abdominal distension. Pleural effusion, ascites, hepatosplenomegaly, and superficial lymphadenopathy were observed and multicentric lymphoma was diagnosed by cytological examination. Immunophenotyping of lymph node and bone marrow using polymerase chain reaction for antigen receptor rearrangement identified a stage V lymphoma originating from T-cell. Despite of systemic chemotherapy using L-asparagenase, vincristine, cyclophoaphamide and prednisolone, neurologic deficits came out and progressed. Cerebrospinal fluid analysis revealed neoplastic lymphocytic pleocytosis indicating central nervous system involvement of lymphoma. The postmortem diagnosis was confirmed based on the histology and imunohistochemistry.

• Proceedings of the Korea Concrete Institute Conference
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• 2010.05a
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• pp.83-84
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• 2010

Precast beam column joints with thru-connectors are developed from precedent study. The seismic performance is evaluated by experimental method. The test results of the precedent study showed that failure modes for all specimens were a compression failure by characteristics of unbonded tendon. Thus, variable considered in the research program for a tensile failure include the use of dog-boned longitudinal steel and concrete confined with steel spirals. The analysis of structural characteristics and evaluation of seismic performance of specimens was conducted by the experimental way. Comparison of result with the test specimens indicates that seismic performance is higher than the precedent study due to concrete confinement effect from steel spirals.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2008.05a
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• pp.99-99
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• 2008