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Effects of Cadmium on Glucose Transport in 3T3- L1 adipocytes  

Kang Donghee (College of Pharmacy, Seoul National University)
Khil Lee-Yong (Lab of Viral and Immunopathogenesis of Diabetes, Julia McFarlane Diabdetes Research Center and Dep. of MID, Faculty of Medicine, University of Calgary)
park Kwangsik (College of Pharmacy, Dongduk Women's University)
Lee Byung-Hoon (College of Pharmacy, Seoul National University)
Moon Chang- Kiu (College of Pharmacy, Seoul National University)
Publication Information
Environmental Analysis Health and Toxicology / v.20, no.1, 2005 , pp. 87-95 More about this Journal
Abstract
Cadmium is well known as a toxic metal and has insulin mimicking effects in rat adipose tissue. This study was undertaken to investigate the effect of CdCl₂ on glucose transport and its mechanism in 3T3 - L1 adipocytes. CdCl₂ exhibits respectively 2.2 and 2.8 fold increases in the 2-deoxyglucose uptake when exposed to 10 and 25 μM of CdCl₂ for 12 hr. To investigate the stimulating mechanism of glucose transport induced by CdCl₂. Wortmannin and PD98059 were used respectively as PI3K inhibitor and MAPK inhibitor, which did not affect 2-DOG uptake. This results suggest that induced 2-deoxy-(l-3H)-D-glucose (2-DOG) uptake by CdCl₂ may not be concerned with the insulin signalling pathway. Whereas nifedipine, a calcium channel blocker inhibited the 2- DOG uptake stimulated by CdCl₂. In addition, we also measured the increased production of Reactive oxygen substances (ROS) and glutathione (GSH) level in 3T3-L1 adipocytes to investigate correlation between the glucose uptake and increased production of ROS with H2DCFDA. CdCl₂ increased production of ROS. Induced 2-DOG uptake and increased production of ROS by CdCl₂ were decreased by N-acetylcystein (NAC). And L-buthionine sulfoximine (BSO) a potent inhibitor of γ-GCS, decreased of 2-DOG uptake. Also NAC and BSO changed the cellular GSH level, but GSH/GSSG ratio remained unchanged at 10, 25 μM of CdCl₂.
Keywords
cadmium; 3T3-L1 adipocytes; reactive oxygen species; glutathione; redox state;
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