• 한국식품영양학회지
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• 제26권3호
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• pp.352-357
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• 2013

This study estimates the classification criteria which distinguishes the types of omega-3 health functional foods, fish oils and fish oil usages through $^{13}C$-NMR spectra and fatty acids contents analysis. The major fatty acids of omega-3, eicosapentaenoic acid (EPA, $C_{20:5}$) and docosahexaenoic acid (DHA, $C_{22:6}$) are being analyzed. 10 ethyl ester (EE) forms and 10 triglyceride (TG) forms are the most common types of fish oils for 20 omega-3 products. Gas chromatography (GC) analysis generally shows the matching EPA and DHA contents of the products listed on the notation. But EE form contents of EPA and DHA are higher and are more varied than the TG form. Most of the samples of EPA/DHA ratio show different content ratios of indicated on the products when comparing with standards. The $^{13}C$-NMR analysis of EPA and DHA on sn-1,3 and sn-2 carbonyl peak position with fish oil triglycerides display whether the reconstituted triglycerides (rTG) are being confirmed or not. As a result of the 9 TG form, the 10 TG products showed similar values: EPA sn-1, 3; 13.46~15.66, sn-2; 3.00~4.52, DHA sn-1, 3; 2.43~4.40, sn-2; 3.84~6.36. But one product showed lower contents (EPA: sn-1, 3; 5.88, sn-2; 2.86, DHA sn-1, 3; 2.29, sn-2; 5.95) of EPA, thus it can be considered a different type of oil and only matched six products according to the label. This study is intended to provide basic materials which identify the status for the types and quality of omega-3 fish oil products according to fatty acids profiles and the $^{13}C$-NMR spectrum confirmed the location specificity of EPA and DHA.

• KSBB Journal
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• 제15권1호
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• pp.72-75
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• 2000

Candida cylindracea 유래의 효소 Lipase-OF 360,000을 사용하여 물고기 기름을 구성하고 있는 각 지방산의 가수분해 특성을 규명하여 보았다. 물고기 기름을 구성하고 있는 다양한 지방산중 $C_{14.0}$, $C_{16.0}$ 및 $C_{18.0}$의 포화지방산과 이중결합이 하나인 $C_{16.1}$, $C_{18.1}$(n-7), $C_{18.1}$(n-9), $C_{20.1}$ 및 $C_{22.1}$의 불포화 지방산은 $\omega$-3 다중불포화지방산에 비하여 쉽게 가수분해되었다. $\omega$-3다중불포화지방산중 탄소수는 동일하나 불포화도가 상이할 경우 불포화도가 낮은 지방산이 불포화도가 높은 지방산보다 쉽게 가수분해되는 특성을 나타내었으며 불포화도는 동일하나 탄소수가 다른 경우 탄소수가 적은 지방산이 탄소수가 많은 지방산보다 쉽게 가수분해되었다. $\omega$-3 다중불포화지방산중 가수분해 반응 후 모노-, 디- 및 트리-클리세라이드 혼합물에 가장 많이 농축되는 지방산은 DHA로 물고기 기름을 구성하는 총 $\omega$-3 지방산의 31.87%에서 가수분해반응 120시간 후에는 글리세라이드 혼합물을 구성하는 총 $\omega$-3 지방산의 51.89%까지 증가하였다.

• Journal of Nutrition and Health
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• 제32권5호
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• pp.526-532
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• 1999

The aim of this study was to observe whether the dietary supplementation of docosahexaenoic acid(DHA). In growing rats requires extra supplementation of arachidonic acid(AA) for brain development. Sprague-Dawley rats were divided into three groups, each fed a different diet. In the FO group, dams were fed a DHA-rich FO diet during pregnancy and lactation and pups were fed the same diet until 10 weeks old. In the AO group dams and pups were similarly fed a FO diet after weaning. DHA and AA were most effetively deposited in the developing brain during pregnancy and lactation in rats. However, FO-W pups showed significantly lower level of DHA at 0-3 weeks compared with the FO and AO groups and than slowly increased DHA levels to about 87% of other groups at 10 weeks with the introduction of the FO diet after weaning. The total amount of DNA in whole brain rapidly reached a maximum level at 3 weeks and then was sustained at a constant level after 5 weeks of age. The DNA content was positively correlated with DHA level but not with AA level in the developing brain. DNA content was significantly lower in the FO-W group compared to the FO and AO group at 3 weeks of age. However, the DNA content of brain in FO-W pups increased to 80% of the FO group level at 10 weeks after feeding the FO diet after weaning. The relative percentage of AA in brain lipids was significantly reduced in the early stage of brain development when only DHA was supplemented. However, DHA supplementation had no significant effect on the incorporation of AA when the approximately 35% of LA in the FO diet was substituted by preformed AA. These results suggest that large quantities of DHA could interfere with the normal conversion of LA to AA if LA is not supplemented enough together with DHA. Therefore, high DHA supplementation may require preformed AA in the diet even though AA has no significant correlation with the DNA content in brain. DHA supplementation after weaning also improved the incorporation of DHA into brain and content of DNA even though brain development was almost completed, suggesting that a low level of DHA supplementation without AA addition might be necessary to improve brain development during infancy as well as during pregnancy and lactation.

• Journal of Nutrition and Health
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• 제30권4호
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• pp.415-424
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• 1997

Serum lipid levels and fatty acid composition of serum phospholipids(PL) were investigated in 24 healthy male subjects who consumed either corn oil(CO) rich In linoleic acid(LA), perilla oil(PO) rich in $\alpha$-linolenic acid($\alpha$-LNA), or canola oil acid(OA) as a major fat source fir 5 weeks. The PO and the CNO groups showed significant(P <0.05) increases in serum high density lipoprotein-cholesterol(HDL-C) levels and in ratios of HDL- C/total cholesterol(TC) compared with initial values measured at the beginning of the study. Significantly(p <0.05) increased concentrations of serum triglycerides(TG) ware observed after 5 weeks of the CO based diet compared with both its intial value and the concentration observed after 5 weeks with the PO-based diet. fatty acid composition of serum PL reflected changes in dietary fatty acid composition and metabolism. Compared with the initial levels, significandy increased contents of eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) were observed in serum PL of the PO group and significantly increased contents of $\alpha$-LNA and EPA were observed in the CNO group. Arachidonic acid(AA) content of serum PL did not change in the CO group during the study period, although, the increase in LA was significant(P <0.05). Compared with the CO-based diet, both the PO and the CNO-based diets seem to have beneficial effects on atherosclerosis by influencing the serum lipid profile and fatty acid composition of serum PL.

• 기술사
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• 제34권5호
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• pp.82-88
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• 2001

Mackerel(Scomber japonicus), pacific saury (Cololabis saira) and sardine(Sardinops melanosticta) is widely distributed in coastal seawater of Korea, these fishes are not effective utilization as processing material cause by rapid lipid oxidation and off flavour. This study was attempted to lipid distribution in body section, whole body, meat, viscera, skin and head, and fatty acid composition of the oils obtained from these body section. The content of total lipid of mackerel, pacific saury and sardine were 12.48%, 12.79% and 13.81% respectively, and lipid contents in different body section of mackerel was muscle 2.31%, viscera 3.54%, skin 1.43% and head 5.20%, while in cause of sardine was muscle 4.17%, viscera 3.15%, skin 1.72%, and head4.77%. The major saturated fatty acids of mackerel, pacific saury and sardine oil were C$\_$16:0/(palmitic acid), C$\_$18:0/(stearic acid), C$\_$14:0/(myristic acid), and monoenoic acids was C$\_$18:0/(oleic acid), C$\_$16:1/ (palmitolic acid), C$\_$22:0/(erucic acid) and C$\_$20:1/(gadoleic acid), in cause of polyenoic acid was C$\_$22:6/(DHA, docosahexaenoic acid), C$\_$20:0/(EPA, eicosapentaenoic acid), C$\_$22:4/(behenic acid), C$\_$18:2/(linoleic acid) high quantity in order. When fresh oil extracted from mackerel, pacific saury and sardine was stored for 20 days at 5$\^{C}$, carbonyl and acid value of oil increased with storage day, but peroxide value decreased after 15 days.

• 대한지역사회영양학회지
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• 제8권2호
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• pp.192-201
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• 2003

The purposes of this research were to assess dietary fatty acid patterns and to elucidate the relationship between the serum cholesterol levels and dietary fatty acid patterns, plasma fatty acid compositions, BMI (body mass index), and other lipid profile. The subjects were 151 adults aged 23 to 80 years, selected from the Outpatient Clinic and Cardiova-scular Department of the Seoul Municipal Hospital. Dietary data were obtained using three day food records. Sixteen dietary fatty acids were analyzed using Korean and US nutrient databases. The subjects were divided into three serum cholesterol levels: desirable (< 200 mg/dl, N = 44), borderline-risk ($\geq$ 200 - < 240 mg/dl, N = 35), and high-risk ($\geq$ 240 mg/dl, N = 72) groups. The high-risk group had higher BMI, waist, and waist to hip ratio (WHR) than the desirable and borderline-risk groups. Serum concentrations of triglyceride, LDL cholesterol and LDL/HDL cholesterol ratio were significantly higher in the high-risk group as compared to those in the other two groups. The serum cholesterol levels were highly correlated with BMI (r = 0.435), triglyceride (r = 0.425) and LDL/HDL cholesterol (r = 0.870) ratio. The highest fatty acid intake was from oleic acid (33 - 34% of total fatty acid intakes), which was followed by linoleic acid (27%), palmitic acid (19%), and stearic acid (7%). There was no correlation between the serum cholesterol levels and the dietary fatty acid intakes, polyunsaturateumonounsaturateusaturated fatty acids (P/M/S) and $\omega$6/$\omega$3 ratios. The correlation between plasma fatty acids such as myristic acid, oleic acid, linoleic acid, and docosahexaenoic acid and serum cholesterol levels was also weak. (Korean J Community Nutrition 8(2) : 192~201, 2003)

• 한국축산식품학회지
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• 제35권3호
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• pp.286-292
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• 2015

Fatty acid (FA) composition of longissimus dorsi intramuscular fat in Black Yak and Chinese Yellow Cattle were evaluated in 44 Black Yak and 41 Chinese Yellow Cattle of both genders. Interactions of species with gender were observed for total saturated fatty acid (SFA), unsaturated fatty acid (UFA), monounsaturated fatty acid (MUFA), polyunsaturated fatty acid (PUFA), palmitic acid, stearic acid, oleic acid, linolenic acid, arachidonic acid, EPA, and DHA concentrations, as well as PUFA/SFA ratio in the longissimus dorsi (p<0.05). The SFA percentage was greater in yellow cattle than yak in both genders but the species difference in heifers was greater than in steers (p<0.05). Yak had greater UFA, MUFA and PUFA percentages than yellow cattle in both steers and heifers (p<0.05) but the difference between yak and yellow cattle heifers was greater than yak and yellow cattle steers. The percentages of inolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid; and PUFA/SFA were greater in yak than yellow cattle in both steers and heifers (p<0.05). In addition, the ratio of n-6/n-3 PUFA in yak was lesser than in yellow cattle (p<0.05). These results indicated that FA composition generally differed between yak and yellow cattle but the differences were not the same in heifers as compared to steers. Results also suggested that species differences in FA composition tended to favor Black Yak over Chinese Yellow Cattle, indicating that the longissimus dorsi of Black Yak may have a higher nutritive value than that of Chinese Yellow Cattle and potential for development as a desirable natural product.

• Journal of Korean Biological Nursing Science
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• 제19권3호
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• pp.141-150
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• 2017

Purpose: The purpose of this study was to investigate the effects of the Korean Dietary Approaches to Stop Hypertension (DASH) education program on oxidative stress, antioxidant capacity, and serum homocysteine (Hcy) level in elderly Korean women. Methods: This was a quasi-experimental study employing non-equivalent control group pretest-posttest design. The DASH group (n= 27) underwent the DASH education program for 12 weeks, whereas the control group (n= 21) was provided one education session. The survey was conducted before and after the intervention period. Results: In DASH group, monounsaturated fatty acid (MUFA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) intakes significantly increased after the intervention as compared to prior to the intervention. No significant differences were found in the control group. After the intervention, thiobarbituric acid reactive substance (TBARS) and Hcy levels significantly decreased in the DASH group but not in the control group. Conclusion: The DASH education program was effective in reducing oxidative stress and Hcy levels in elderly Korean women.

• KSBB Journal
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• 제14권1호
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• pp.109-113
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• 1999

이산화탄소를 용매로하는 초임계유체 크로마토그래퍼(SFC)방법을 이용하여 어유로부터 EPA및 DHA를 고순도로 분리정제하는 연구를 하였다. 질산은 칼럼을 이용하는 초임계 이산화탄소의 초기 압력조건 변화가 저 지방산의 용해도 및 분리도에 미치는 영향을 조사하였는데 압력이 증가될수록 저지방산의 용해도는 증가되었고, 반면에 EPA와의 분리도는 감소하는 경향을 나타내었다. EPA의 분리정제 효율을 높이기 위해 초임계 이산화탄소에 대한 단계별 밀도구매 방법을 적용하였다. 적용 결과 초기에 저지방산이 먼저 분리되었고, 계속해서 92.1%~97.8%EPA 순도를 갖는 분획들을 얻을 수 있었다. 순도가 높은 3개의 분획의 평균 순도는 95.6%이었고 회수율은 30%에 달하였다.

• 한국환경과학회지
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• 제23권1호
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• pp.47-52
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• 2014

Antarctic krill (Euphausia superba) is typical of a enormous biomass of marine zooplankton that could provide good nutrition in human body. This study was conducted to identify the nutrition of krill, a live in Antarctic Ocean. The analysis result of fatty acids of krill meal was as follow. The compositions of fatty acid were saturated fatty (SFA) acid 41.41%, monounsaturated fatty acid (MUFA) 21.69%, polyunsaturated fatty acid (PUFA) 36.89%, and p/s ratio was 0.89. The major fatty acids in all parts were eicosapentaenoic acid (EPA, 21.54%), palmitic acid (27.51%), oleic acid (13.35%) and docosahexaenoic acid (DHA, 12.42%). Especially, EPA and DHA were occupied 33.96% of polyunsaturated fatty acid. The mineral contents of krill meal were calcium 24477.21 mg $kg^{-1}$, sodium 14728.69 mg $kg^{-1}$, magnesium 6973.49 mg $kg^{-1}$, potassium 3981.67 mg $kg^{-1}$, iron 395.33 mg $kg^{-1}$ and manganese 5.74 mg $kg^{-1}$. The contents of major vitamin were retinol 86717.37 ${\mu}g$ RE, ${\beta}$-carotene 44.87 ${\mu}g$ RE, tocopherol 2.60 mg, pantothenic acid 1.61 mg, indicating that krill meal contains large amount of retinol and ${\beta}$-carotene.