• 제목/요약/키워드: Division of Fraction

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한지에서 셀룰로오스 분해 미생물에 대한 세신 추출물의 저해 활성 (Inhibitory Activity of Asarum sieboldii against Wood Rot Fungi on Traditional Paper, Hanji)

  • 홍진영;김영희;조창욱;이정민;김수지;정소영
    • 한국약용작물학회지
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    • 제25권5호
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    • pp.282-289
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    • 2017
  • Background: To investigate the possibility of using Asarum sieboldii as an environment-friendly fumigant for protecting organic cultural heritages, the inhibitory effect of A. sieboldii extract against wood rot fungi on Hanji was examined. Methods and Results: The physical, optical, and morphological properties of Hanji inoculated with Trametes versicolor and Tyromyces palustris, and exposed to the n-hexane fraction of A. sieboldii extract, were measured. The physical properties were expressed as weight loss, zero-span tensile strength and viscosity and the optical properties were depicted by luminance and chromaticity ($L^*$, $a^*$, and $b^*$). The results showed that, the n-hexane fraction of A. sieboldii extract inhibited the growth of fungi on Hanji, and preserved its condition. At a concentration of 25 mg, the n-hexane fraction of A. sieboldii extract maintained zero-span tensile strength, increased viscosity, and restricted discoloration of Hanji. It also was confirmed that the weight of fungi infested Hanji exposed to the extract did not decrease. Scanning electron microscopic images revealed that the spores and hyphae of T. versicolor and T. palustris were not present on Hanji during treatment with > 25 mg of the n-hexane fraction of A. sieboldii extract. Conclusions: These results indicate that the n-hexane fraction of A. sieboldii extract by virtue of its antifungal effectiveness may help in preserving Korean paper cultural heritages, including Hanji.

Effect of Soil Respiration on Light Fraction-C and N Availability in Soil Applied with Organic Matter

  • Ko, Byong-Gu;Lee, Chang-Hoon;Kim, Myung-Sook;Kim, Gun-Yeob;Park, Seong-Jin;Yun, Sun-Gang
    • 한국토양비료학회지
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    • 제49권5호
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    • pp.510-516
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    • 2016
  • Soil respiration has been recognized as a key factor of the change of organic matter and fertility due to the carbon and nitrogen mineralization. In this study, we evaluated the effect of soil respiration on the light fraction-C and inorganic N content depending on temperature in soil applied with organic matter. Soil respiration was calculated by using total $CO_2$ flux released from soil applied with $2Mg\;ha^{-1}$ of rice straw compost and rye for 8 weeks incubation at 15, 25, $35^{\circ}C$ under incubation test. After incubation test, light fraction and inorganic N content were investigated. Rye application dramatically increased soil respiration with increasing temperature. $Q_{10}$ value of rye application was 1.69, which was higher 27% than that of rice straw compost application. Light-C and $NO_3-N$ contents were negatively correlated to soil respiration. Light-C in rye application more decreased than that in rice straw compost with temperature levels. These results indicate that temperature sensitivity of soil respiration could affect soil organic mater content and N availability in soil due to carbon availability. Also, light fraction would be useful indicator to evaluate decomposition rate of organic matter in soil under a short-term test.

Different level of tumor necrosis factor-α expression after administration of silk sericin fraction in RAW264.7 cells

  • Kim, Dae-Won;Jo, You-Young;Kweon, HaeYong;Kim, Seong-Gon
    • International Journal of Industrial Entomology and Biomaterials
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    • 제41권1호
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    • pp.1-5
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    • 2020
  • Tumor necrosis factor-α (TNFα) is a representative marker for inflammation. Silk sericin is known as mild TNFα inducer. The purpose of this study was to compare the level of TNFα among different fractions of silk sericin. Silk sericin was extracted from cocoon and separated it by molecular weight. Each fraction was applied to RAW264.7 cells. The level of TNFα was evaluated by western blot and ELISA assay. In results, the level of TNFα was increased as time-dependent manner. Higher molecular weight fraction of sericin induced higher amount of TNFα than lower molecular weight fraction. In conclusion, different molecular weight fraction of sericin induced TNFα differently.

예비 초등 교사들의 분수 나눗셈에 대한 지식 분석 (An Analysis on the Prospective Elementary Teachers' Knowledge in the Case of Division of Fractions)

  • 방정숙
    • 한국수학교육학회지시리즈A:수학교육
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    • 제47권3호
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    • pp.291-310
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    • 2008
  • This article is based on an international collaborative study that aimed to investigate mathematical preparation of prospective elementary teachers in several selected education systems in East Asia. This article reports the Korean portion of the study. A survey instrument was developed to explore not only prospective teachers' knowledge of elementary mathematics curriculum and their beliefs in their preparation and mathematics instruction but also their subject matter knowledge and pedagogical content knowledge on the topic of fraction division. A total of 291 seniors in 3 universities participated in the survey. The results reveal these prospective teachers' strengths and weaknesses with regard to their knowledge of fraction division, and suggest that content-specific pedagogical knowledge needs to be emphasized in the teacher preparation program.

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원양산 오징어(Illex argentinus) 내장으로부터 Endoprotease의 분획 (Fractionation of Endoprotease from Viscera of the Argentina Shortfin Squid Illex argentinus)

  • 김혜숙;김진수;허민수
    • 한국수산과학회지
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    • 제41권3호
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    • pp.176-181
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    • 2008
  • To evaluate the effective use of endoprotease from squid viscera as a food processing aid, various methods of fractionating endoprotease from viscera of the Argentina shortfin squid (Illex argentinus) were evaluated. The endoprotease-positive fractions of each fractionation were fraction II (30-40%, w/w) with cold acetone, fraction IV (50-60% saturation) with ammonium sulfate, fraction UF with anion exchange chromatography, and fraction II (15-24 kDa) with gel filtration. The specific activities (approximately 25 U/mg) of the fractions using ammonium sulfate and gel filtration were higher than the others. Total azocaseinolytic activity and recovery of the positive fraction using gel filtration were 806.95U and 37.82%, respectively, and were the highest among the positive fractions. Based on the results, gel filtration was the most efficient method for fractionating endoprotease from the viscera of Illex argentinus.

Mechanical Properties of Unidirectional Carbon-carbon Composites as a Function of Fiber Volume Content

  • Dhakate, S.R.;Mathur, R.B.;Dham, T.L.
    • Carbon letters
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    • 제3권3호
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    • pp.127-132
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    • 2002
  • Unidirectional polymer composites were prepared using high-strength carbon fibers as reinforcement and phenolic resin as matrix precursor with keeping fiber volume fraction at 30, 40, 50 and 60% respectively. These composites were carbonized at $1000^{\circ}C$ and graphitised at $2600^{\circ}C$ in the inert atmosphere. The carbonized and graphitised composites were characterized for mechanical properties as well as microstructure. Microscopic studies were carried out of the polished surface of carbonized and graphitised composites after etching by chromic acid, to understand the effect of fiber volume fraction on oxidation at fiber-matrix interface. It is found that the flexural strength in polymer composites increases with fiber volume fraction and so does for the carbonised composites. However, the trend was found to be reversed in graphitised composites. In all the carbonized composites anisotropic region has been observed at fiber-matrix interface which transforms into columnar type microstructure upon graphitisation. The extension of strong and weak columnar type microstructure is function of fiber volume fraction. SEM microscopy of the etched surface of the sample reveal that composites containing 40% fiber volume has minimum oxidation at the interface, revealing a strong interfacial bonding.

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분수 나눗셈의 통합적 이해를 위한 방편으로서 포함제에서 1÷(제수)를 매개로 하는 방법에 대한 고찰 (On the Method of Using 1÷(divisor) in Quotitive Division for Comprehensive Understanding of Division of Fractions)

  • 임재훈
    • 한국초등수학교육학회지
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    • 제22권4호
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    • pp.385-403
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    • 2018
  • 분수 나눗셈의 여러 맥락 중 등분제와 카테시안 곱의 역 맥락에서는 제수의 역수 곱하기 알고리즘이 자연스럽게 유도된다. 그러므로 제수의 역수 곱하기 알고리즘을 분수 나눗셈의 통합 알고리즘으로 지도하고자 할 때 특히 이슈가 되는 것은 포함제 맥락이다. 이 논문에서는 포함제 맥락에서 $1{\div}$(제수)를 매개로 하는 방법이 지닌 잠재력 및 그 기반을 분석하고, 이 방법을 제수의 역수 곱하기 알고리즘을 분수 나눗셈의 통합 알고리즘으로 지도하려 할 때 고려할 수 있는 한 대안으로 제안한다. 포함제 맥락에서 $1{\div}$(제수)를 매개로 하여 제수의 역수 곱하기 알고리즘을 유도하는 방법은 다음과 같은 특징을 지니고 있다. 첫째, 포함제 맥락에서 맥락과의 연결성을 유지한 채로 제수의 역수 곱하기 알고리즘을 유도할 수 있다. 둘째, 다른 맥락들에서와 마찬가지로, 제수와 1의 곱셈적 관계에 주목한다. 셋째, 다른 맥락들에서와 마찬가지로, 제수와 1의 곱셈적 관계를 1/제수의 분모을 징검다리로 삼는 추론과 제수의 분자를 징검다리로 삼는 두 가지 추론으로 파악한다. 이러한 특징은 이 방법이 제수의 역수 곱하기 알고리즘을 분수 나눗셈의 공통 구조를 담고 있는 통합 알고리즘으로 다루는 데 기여할 수 있음을 시사한다. 한편, 이 방법은 양분수의 이중적 의미와 배의 합성을 그 기반으로 한다. 분수 나눗셈의 통합적 이해를 지향하는 교재 개발 및 수업 연구에서는 이 기반의 형성에 유의할 필요가 있다.

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SiCf/SiC 복합체 튜브의 표면조도 및 섬유 부피 분율에 미치는 필라멘트 와인딩 방법의 영향 (Effect of Filament Winding Methods on Surface Roughness and Fiber Volume Fraction of SiCf/SiC Composite Tubes)

  • 김대종;이종민;박지연;김원주
    • 한국세라믹학회지
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    • 제50권6호
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    • pp.359-363
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    • 2013
  • Silicon carbide and its composites are being considered as a nuclear fuel cladding material for LWR nuclear reactors because they have a low neutron absorption cross section, low hydrogen production under accident conditions, and high strength at high temperatures. The SiC composite cladding tube considered in this study consists of three layers, monolith CVD SiC - $SiC_f$/SiC composite -monolith CVD SiC. The volume fraction of SiC fiber and surface roughness of the composite layer affect mechanical and corrosion properties of the cladding tube. In this study, various types of SiC fiber preforms with tubular shapes were fabricated by a filament winding method using two types of Tyranno SA3 grade SiC fibers with 800 filaments/yarn and 1600 filaments/yarn. After chemical vapor infiltration of the SiC matrix, the surface roughness and fiber volume fraction were measured. As filament counts were changed from 800 to 1600, the surface roughness increased but the fiber volume fraction decreased. The $SiC_f$/SiC composite with a bamboo-like winding pattern has a smaller surface roughness and a higher fiber volume fraction than that with a zigzag winding pattern.

정수처리 공정에서 용존 유기물질 분류에 의한 haloacetic acid 생성능 평가 (Evaluation of Haloacetic Acid Formation Potential in Drinking Water Treatment Process by Fraction Technique)

  • 손희종;황영도;류동춘;정철우;이건;손형식
    • 한국환경과학회지
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    • 제23권9호
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    • pp.1655-1662
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    • 2014
  • A comprehensive fractionation technique was applied to a set of water samples obtained along drinking water treatment process with ozonation and biological activated carbon (BAC) process to obtain detailed profiles of dissolved organic matter (DOM) and to evaluate the haloacetic acid (HAA) formation potentials of these DOM fractions. The results indicated that coagulation-sedimentation-sand filtration treatment showed limited ability to remove hydrophilic fraction (28%), while removal of hydrophobic and transphilic fraction were 57% and 40%, respectively. And ozonation and BAC treatment showed limited ability to remove hydrophobic fractions (6%), while removal of hydrophilic and transphilic fractions were 25% and 18%. The haloacetic acid formation potential (HAAFP)/dissolved organic carbon (DOC) of hydrophilic fraction was the highest along the treatment train and HAAFP/DOC of hydrophilic fraction was higher than hydrophobic and transphilic fraction as 23%~30%, because of better removal for hydrophobic fraction both in concentration and reactivity.

Comparative GC-MS Based In vitro Assays of 5α-Reductase Activity Using Rat Liver S9 Fraction

  • Lee, Su-Hyeon;Lee, Dong-Hyoung;Lee, Jeong-Ae;Lee, Won-Yong;Chung, Bong-Chul;Choi, Man-Ho
    • Mass Spectrometry Letters
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    • 제3권1호
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    • pp.21-24
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    • 2012
  • $5{\alpha}$-Dihydrotestosterone (DHT) is the primary active metabolite of testosterone, catalyzed by $5{\alpha}$-reductase ($5{\alpha}R$) in the skin, prostate, and liver. In this study, the $5{\alpha}R$ activity in rat liver S9 fraction in the presence of a NADPH-generating system was evaluated and compared by gas chromatography-mass spectrometry (GC-MS)-based in vitro assays. Testosterone and a $5{\alpha}R$ inhibitor, finasteride, were added to the S9 fractions and incubated at $37^{\circ}C$ for 1 h. Both testosterone and DHT were quantitatively measured and compared with two different GC-MS-based steroid profiling techniques. DHT was not detected by conventional GC-MS analysis in the absence of finasteride when the concentration of testosterone in the S9 fraction was less than $0.2{\mu}M$, whereas the isotope-dilution GC-MS (GC-IDMS) system was able to evaluate the $5{\alpha}R$ activity. Because the S9 fraction contains more reactive enzymes and is easier to collect from tissues compared with a microsomal solution, the combination of the S9 fraction and GC-IDMS technique may be a promising assay for evaluating the $5{\alpha}R$ activity in large-scale clinical studies.