• ALGAE
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• v.22 no.4
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• pp.253-259
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• 2007

This study deals with the taxonomy of the marine diatom Ditylum brightwellii (West) Grunow: shape, size and ratio, marginal ridge, labiate process, areolation, and seasonal distribution are all considered. Ditylum brightwellii were divided into two type; prism- and cylinder-shaped. Comparisons between specimens from the Yellow Sea were made on, (1) shape- prism types are prismatic-shaped and/or regular square or right-angled tetragon, cylinder types are cylinder-shaped and/or long right-angled tetragon in the girdle view, respectively; (2) size and ratiodiameters of prism types are 82.1 ± 2 μm, while those of cylinder types are 21.2 ± 5 μm and ratio of pervalvar axis/diameter- prism types cells are 1.0-1.5, but cylinder types are 3.9-5.5; (3) marginal ridges- prism types have dotted, while those of the cylinder-type cells have piece, slotted or fimbriated on the marginal ridge; (4) labiate process- both prism- and cylinder-types are central part in valve face, but length of labiate processes of prism types are longer than those of cylinder types; (5) cell margin- the girdle margin show many shallow furrows in prism type and smooth in cylinder type; (6) areolation- both prism- and cylinder-types are a radial pattern in the valve center, but areolae of prism types are larger than those of cylinder types; (7) seasonal distribution- cylinder types are dominated in summer and prism types in the other seasons.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.306-312
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• 2016

Microalgae are the potential bioindicators of environmental changes, for the environmental risk assessment as well as to set limits for toxic chemical release in the aquatic environment. Here, we evaluated the effects of two endocrine disrupting chemicals (EDCs), namely bisphenol A (BPA) and Aroclor 1016, on the green algae Tetraselmis suecica, diatom Ditylum brightwellii, and dinoflagellate Prorocentrum minimum. Each species showed wide different sensitivity ranges when exposed to these two EDCs; the 72 h effective concentration ($EC_{50}$) for these test species showed that Aroclor 1016 was more toxic than BPA. $EC_{50}$ values for the diatom D. birghtwellii were calculated at 0.037 mg/L for BPA and 0.002 mg/L for Aroclor 1016, representing it was the most sensitive when compared to the other species. In addition, these results suggest that these EDC discharge beyond these concentrations into the aquatic environments may cause harmful effect to these marine species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.3
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• pp.359-367
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• 1999

For investigating the activities of algicidal bacteria, the variations of algicidal bacterial population and chlorophyll-a were checked weekly in Chindong Bay, Korea from May to July, 1998. For identifying their killing characteristics, three strains were selected from the isolated algicidal bacteria. The density of algicidal bacteria kept changing in the range of $6.0\times10^1$ to $6.4\times10^5$ cell $\ell^{-1}$. The density flux of algicidal bacteria coincided with that of chlorophyll-a by a week of lag time. Three algicidal bacteria isolated from field strains, H519S5-4, H605S5-15 and H605S5-22, were investigated in nine microalgal species, Heterosigma akashiwo, Chattonella sp. (Raphidlphyceae), Gymnodinium catenatum, Gyrodinium impudicum, Cochlodinium polyklikoides (Dinophyceae), Chaetoceros sp., Coscinodiscus granii, Ditylum brightwellii, Thalassiosira rotula (Bacillariophyceae). Strain H605S5-22 showed a wide algicidal activities over nine microlgae, strain H605S5-15 over H. akashiwo, G. catenatum, T. rotula, Chattonella sp. and strain H519S5-4 over H. akashiwo, Chattonella sp., Chaetoceros sp., G. catenatum. The activities of the three strains were detected by the secretion of algicidal substances. Therefore, it is suggested that the activities of algicidal bacteria have a significant influence over the population dynamics of phytoplankton and get involved with the sharp decrease in red tides in the coastal area.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.4
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• pp.223-231
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• 2014

In order to prevent the spread of non-indigenous aquatic species through the ballast water in commercial ships, International Maritime Organization (IMO) adopted in 2004 the International Convention for Control and Management of Ship's Ballast Water and Sediments. The Convention mandates treatment of ballast water for most transoceanic voyages and its confirmation of treatment is made with plankton live/dead assay. Fluorescein diacetate assay (FDA), which produces bright green light for live phytoplankton, has been a de facto standard method to determine the survival of marine plankton, but its staining efficacy has been in dispute. In the present study, we examined the limitation of FDA, and compared its efficacy with Neutral red (NR) staining, another promising assay and widely used especially for zooplankton mortality. For all phytoplankton species studied in the present study, except Ditylum brightwellii, the staining efficiency was <50% with FDA. The green FDA fluorescence interfered with phytoplankton autofluorescence in most samples. In contrast, NR assay stained over 90% of both phytoplankton and zooplankton species tested in this study. FDA assay also showed that green FDA fluorescence rapidly faded when phytoplankton cells were exposed to microscope light. Both FDA and NR assay were negative on formalin-killed individuals of both phytoplankton and zooplankton species. Our results suggest that NR assay is more effective for determining the survival of marine plankton and can be applied to test the efficacy of ballast water treatment.

• ALGAE
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• v.23 no.1
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• pp.53-61
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• 2008

In order to understand variation and relationship between standing crops and biomass of phytoplankton dominant species for the long term periods, this study was seasonally investigated in the marine ranching ground of Tongyeong coastal waters from 2000 to 2007. Total 268 taxa representing 217 Bacillariophyceae, 46 Dinophyceae, 4 Dictychophyceae, 1 Euglenophyceae were observed in phytoplankton communities. Dominant species consisted of 5 species as standard in standing crops: Chaetoceros curvisetus (18.01%), Chaetoceros socialis (12.95%), Skeletonema costatum (8.39%), Chaetoceros compressus (6.87%), Asterionellopsis glacialis (5.02%). However, to determine dominant species as biomass concept, Ditylum brightwellii, Guinardia striata, Rhizosolenia spp. and Skeletonema costatum were occupied with dominant species (19.67%). As determining for cell sizes, dominant species were divided with two groups such as micro- and nanophytoplankton (standing crops) and mesophytoplankton (biomass). However, Skeletonema costatum in anophytoplankton was associated to affect fluctuation between standing crops and biomass.

• 한국해양학회지
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• v.11 no.1
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• pp.34-38
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• 1976

Monthly observation of phytoplankton were made from September, 1974 to June 1975 at three stations in the Masan Bay. Sixty three species of phytoplankton (diatoms), representing 25 genera were taken in this study. Of these Skeletonema costatum, Eucampis zoodiacus, Ditylum brightwellii, Chaetoceros debilis, Coscinosira polychorda, and Leptocylindrus danicus were the dominant species in this area. By the ecological division the neritic species occupied 85.71% of total diatom diversity and it showed the characteristics of emayment. Diversity index(H) were the lowest in January and May in surface, while the highest in October. Phytoplankton standing crop varied extensively by months; ranging from 3,780 to 7,642,798 cells/l. total phytoplankton standing crop showed three major peaks in September, December and May. After bloom diatom standing crop were decreased gradually and showed minimum in November and February.

• 한국해양학회지
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• v.14 no.1
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• pp.26-31
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• 1979

1976년 7월부터 1978년 5월까지 2년동안 매 기수월에 진해만의 6개 정점에서 식물성플랑크톤군집의 년변화를 조사하였다. 조사기간 중 수온은 2.28$^{\circ}C$~25.48$^{\circ}C$, 염분도는 27.42 ~33.90 , pH는 8.0~8.5, 투명도는 0.5m~0.8m, 용존산소량은 2.23ml/l~12.32ml/l의 분포를 보였다. 식물성플랑크톤의 출현종수는 30속, 79종, 2품종, 3변종으로 나타났다. 이들의 우점종은 Skeletonema costatum, Thalassiosira hyalina, Leptocylindrus danicus, Chaetoceros curvisetus, Chaetoceros costatus, Nitzschia pungens, Nitzschia seriata였으며, Chaetoceros affinis, Chaetoceros curvisetus, Coscinodiscus centralis var. pacifica, Ditylum brightwellii, Navicula distans, Nitzschia pungens, Pleurosigma elongatum, Thalassiorira hyalina, Thalassiothrix frauenfeldii등은 출현빈도에 있어서 이 지역의 대표종이었다. 출현종의 생태적 구분은 연안종이 67.86%로서 내만의 특성을 나타내고 있다. 현존량의 계절적인 변화는 808~12,461,000 cell/l까지 심한 변화폭을 보이고 있으며, 1976년 7월, 11월, 1977년 5월, 9월, 1978년 5월에 $10^{6}$ cells/l대의 대발생이 있었다. 대발생때의 다양성지수는 1976년 7월에 0.5625~1.0345로 낮은 값을 보여 Skeletonema costatum에 의한 대발생이었으며, 11월의 3.0702~4.1019의 높은 값을 보인때는 18~33종의 다양한 출현종에 의한 것이었다.