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Investigation of Bacterial Diversity in Membrane Bioreactor and Conventional Activated Sludge Processes from Petroleum Refineries Using Phylogenetic and Statistical Approaches

  • Silva, Cynthia;Jesus, Ederson C.;Torres, Ana P. R.;Sousa, Maira P.;Santiago, Vania M. J.;Oliveira, Valeria M.
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.447-459
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    • 2010
  • Bacterial diversity of two distinct wastewater treatment systems, conventional activated sludge (CAS) and membrane bioreactor (MBR), of petroleum refineries were investigated through 16S rRNA gene libraries. Sequencing and phylogenetic analysis showed that the bacterial community composition of sludge samples was distinct between the two wastewater treatment systems. MBR clones belonged predominantly to Class Betaproteobacteria, represented mainly by genera Thiobacillus and Thauera, whereas CAS clones were mostly related to Class Alphaproteobacteria, represented by uncultured bacteria related to Order Parvularculales. Richness estimators ACE and Chao revealed that the diversity observed in both libraries at the species level is an underestimate of the total bacterial diversity present in the environment and further sampling would yield an increased observed diversity. Shannon and Simpson diversity indices were different between the libraries and revealed greater bacterial diversity for the MBR library, considering an evolutionary distance of 0.03. LIBSHUFF analyses revealed that MBR and CAS communities were significantly different at the 95% confidence level ($P{\leq}0.05$) for distances $0{\leq}D{\leq}0.20$. This work described, qualitatively and quantitatively, the structure of bacterial communities in industrial-scale MBR and CAS processes of the wastewater treatment system from petroleum refineries and demonstrated clearly differentiated communities responsible for the stable performance of wastewater treatment plants.

Energy spectrum of particles arriving at the ground and S(800) determination by Monte Carlo simulation for Telescope Array

  • Kim, Ji-Hee;Roh, Soon-Young;Ryu, Dong-Su;Kang, Hye-Sung;Kasahara, Katuaki;Kido, Eiji;Taketa, Akimichi
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.2
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    • pp.58.1-58.1
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    • 2010
  • Telescope Array (TA) experiment in Utah, USA, observes ultrahigh-energy cosmic rays (UHECRs); UHECRs refer cosmic rays with energy above $10^{18}eV$. Using COSMOS and CORSIKA, we have produced a library of over 1000 thinned extensive air shower (EAS) simulations with the primary energies ranging from $10^{18.5}eV$ to $10^{20.25}eV$ and the zenith angle of primary cosmic ray particle from $0^{\circ}$ to $45^{\circ}$. Here, we present the energy spectrum of particles arriving at the ground. We have also calculated the detector response evaluated using GEANT4 simulations. Here, we discuss S(800), i.e. the signal at a distance of 800 m from the shower core, as the primary energy estimator.

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Radiation-training system with a custom survey-meter mock-up in a browser-based mixed reality environment

  • Hiroyuki Arakawa;Toshioh Fujibuchi;Kosuke Kaneko;Yoshihiro Okada;Toshiko Tomisawa
    • Nuclear Engineering and Technology
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    • v.56 no.6
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    • pp.2428-2435
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    • 2024
  • Training for radiation protection and control requires a visual understanding of radiation, which cannot be perceived by the human senses. Trainees must also master the effective use of measuring instruments. Traditionally, such training has exposed trainees to radiation sources. Here, we present a novel e-training strategy that enables safe, exposure-free handling of a radiation measuring tool called a survey meter. Our mixed reality radiation-training system merges the physical world with a digital one. Collaborating with a mixed reality headset (HoloLens 2), this system constructs a mock-up of a survey meter in real-world space. The HoloLens 2 employs a browser-based application to visualize radiation and to simulate/share the use of the survey meter, including its physical movements. To provide a dynamic learning experience, the system adjusts the survey-meter mock-up readings according to the operator's movements, distance from the radiation source, the response time of survey meter, and shielding levels. Through this approach, we expect that trainees will acquire practical skills in interpreting survey-meter readings and gain a visual understanding of radiation in real-world situations.

Solution State Structure of pB1, the Mimotopic Peptide of Apolipoprotein B-100, by NMR

  • Lee, Sung-Ran;Kim, Dae-Sung;Kim, Hyo-Joon;Lee, Yong-Woo;Won, Ho-Shik
    • Bulletin of the Korean Chemical Society
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    • v.25 no.12
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    • pp.1845-1849
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    • 2004
  • Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 ${\AA}^2$ and the total RMSD was 0.5-1.5 ${\AA}. Moreover, pB1 structure included a weak $3_{10}$-helix from $Ile^7$,/TEX> to $Trp^{13}$.

Mechanism Studies of Substituted Triazol-1-yl-pyrimidine Derivatives Inhibition on Mycobacterium tuberculosis Acetohydroxyacid Synthase

  • Chien, Pham Ngoc;Jung, In-Pil;Reddy, Katta Venugopal;Yoon, Moon-Young
    • Bulletin of the Korean Chemical Society
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    • v.33 no.12
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    • pp.4074-4078
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    • 2012
  • The first step in the common pathway for the biosynthesis of branched chain amino acids is catalyzed by acetohydroxyacid synthase (AHAS). The AHAS is found in plants, fungi and bacteria. With an aim to identify new anti-tuberculosis drugs that inhibit branched chain amino acid biosynthesis, we screened a chemical library against Mycobacterium tuberculosis AHAS. The screening identified four compounds, AVS 2087, AVS 2093, AVS 2236, and AVS 2387 with $IC_{50}$ values of 0.28, 0.21, 3.88, and $0.25{\mu}M$, respectively. Moreover, these four compounds also showed strong inhibition against reconstituted AHAS with $IC_{50}$ values of 0.37, 0.26, 1.0, and $1.18{\mu}M$, respectively. The basic scaffold of the AVS group consists of 1-pyrimidin-2-yl-1H-[1,2,4]-triazole-3-sulfonamide. The most active compound, AVS 2387, showed the lowest total interaction energy -8.75 Kcal/mol and illustrates its binding mode by hydrogen bonding with $H_{\varepsilon}$ of Gln517 with the distance of $2.24{\AA}$.

A Low-Cost Speech to Sign Language Converter

  • Le, Minh;Le, Thanh Minh;Bui, Vu Duc;Truong, Son Ngoc
    • International Journal of Computer Science & Network Security
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    • v.21 no.3
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    • pp.37-40
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    • 2021
  • This paper presents a design of a speech to sign language converter for deaf and hard of hearing people. The device is low-cost, low-power consumption, and it can be able to work entirely offline. The speech recognition is implemented using an open-source API, Pocketsphinx library. In this work, we proposed a context-oriented language model, which measures the similarity between the recognized speech and the predefined speech to decide the output. The output speech is selected from the recommended speech stored in the database, which is the best match to the recognized speech. The proposed context-oriented language model can improve the speech recognition rate by 21% for working entirely offline. A decision module based on determining the similarity between the two texts using Levenshtein distance decides the output sign language. The output sign language corresponding to the recognized speech is generated as a set of sequential images. The speech to sign language converter is deployed on a Raspberry Pi Zero board for low-cost deaf assistive devices.

Implementation of augmented reality using parallel structure (병렬구조를 이용한 증강현실 구현)

  • Park, Tae-Ryong;Heo, Hoon;Kwak, Jae-Chang
    • Journal of IKEEE
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    • v.17 no.3
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    • pp.371-377
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    • 2013
  • This thesis propose an efficient parallel structure method for implementing a FAST and BRIEF algorithm based Augmented Reality. SURF algorithm that is well known in the object recognition algorithms is robust in object recognition. However, there is a disadvantage for real time operation because, SURF implementation requires a lot of computation. Therefore, we used a FAST and BRIEF algorithm for object recognition, and we improved Conventional Parallel Structure based on OpenMP Library. As a result, it achieves a 70%~100% improvement in execution time on the embedded system.

Lane Marking Detection of Mobile Robot with Single Laser Rangefinder (레이저 거리 센서만을 이용한 자율 주행 모바일 로봇의 도로 위 정보 획득)

  • Jung, Byung-Jin;Park, Jun-Hyung;Kim, Taek-Young;Kim, Deuk-Young;Moon, Hyung-Pil
    • Journal of Institute of Control, Robotics and Systems
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    • v.17 no.6
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    • pp.521-525
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    • 2011
  • Lane marking detection is one of important issues in the field of autonomous mobile robot. Especially, in urban environment, like pavement roads of downtown or tour tracks of Science Park, which have continuous patterns on the surface of the road, the lane marking detection becomes more important ability. Although there were many researches about lane detection and lane tracing, many of them used vision sensors mainly to detect lane marking. In this paper, we obtain 2 dimensional library data of 'Intensity' and 'Distance' using one laser rangefinder only. We design a simple classifier and filtering algorithm for the lane detection which uses only one LRF (Laser Range Finder). Allowing extended usage of LRF, this research provides more functionality not only in range finding but also in lane detecting to mobile robots. This work will be technically helpful for robot developers to design more simple and efficient autonomous driving system using LRF.

Iris Ciphertext Authentication System Based on Fully Homomorphic Encryption

  • Song, Xinxia;Chen, Zhigang;Sun, Dechao
    • Journal of Information Processing Systems
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    • v.16 no.3
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    • pp.599-611
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    • 2020
  • With the application and promotion of biometric technology, biometrics has become more and more important to identity authentication. In order to ensure the privacy of the user, the biometrics cannot be stored or manipulated in plaintext. Aiming at this problem, this paper analyzes and summarizes the scheme and performance of the existing biometric authentication system, and proposes an iris-based ciphertext authentication system based on fully homomorphic encryption using the FV scheme. The implementation of the system is partly powered by Microsoft's SEAL (Simple Encrypted Arithmetic Library). The entire system can complete iris authentication without decrypting the iris feature template, and the database stores the homomorphic ciphertext of the iris feature template. Thus, there is no need to worry about the leakage of the iris feature template. At the same time, the system does not require a trusted center for authentication, and the authentication is completed on the server side directly using the one-time MAC authentication method. Tests have shown that when the system adopts an iris algorithm with a low depth of calculation circuit such as the Hamming distance comparison algorithm, it has good performance, which basically meets the requirements of real application scenarios.

Design and Implementation of Finger Direction Detection Algorithm in YOLO Environment (YOLO 환경에서 손가락 방향감지 알고리즘 설계 및 구현)

  • Lee, Cheol Min;Thar, Min Htet;Lee, Dong Myung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2021.05a
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    • pp.28-30
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    • 2021
  • In this paper, an algorithm that detects the user's finger direction using the YOLO (You Only Look Once) library was proposed. The processing stage of the proposed finger direction detection algorithm consists of a learning data management stage, a data learning stage, and a finger direction detection stage. As a result of the experiment, it was found that the distance between the camera and the finger had a very large influence on the accuracy of detecting the direction of the finger. We plan to apply this function to Turtlebot3 after improving the accuracy and reliability of the proposed algorithm in the future.

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