• Current Research on Agriculture and Life Sciences
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• 제3권
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• pp.112-119
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• 1985

관상식물(觀賞植物)에 병해조사(病害調査) 일환(一環)으로 대구(大邱)와 근교(近郊)의 온실(溫室) 등(等)에 재식(栽植)되어 있는 19과(科) 34속(屬) 34종(種)의 식물(植物)에 대(對)하여 진균(眞菌)에 이병(罹病)된 식물(植物)을 조사(調査)한 결과(結果), Glomerella 속균(屬菌) 등(等) 16속(屬) 병원진균(病原眞菌)에 의(依)하여 32속(屬) 32종(種)의 식물(植物)에서 37종(種)의 병(病)이 발생(發生)하고 있었다. 이들 중 우리나라에서 이제까지 보고(報告)되지 않은 미기록병해(未記錄病害)는 Glomerella속균(屬菌), Colletotrichum속균(屬菌), Ceratocystis속균(屬菌), Pestalotia속균(屬菌), Gloeosporium속균(屬菌), Macrophoma속균(屬菌), Sphaceloma속균(屬菌), Botrytis속균(屬菌), Alternaria속균(屬菌), Phyllosticta속균(屬菌), 및 Phomopsis속균(屬菌) 등(等)에 의(依)하여 19속(屬) 19종(種)의 관상식물(觀賞植物)에 22종(種)의 병(病)이 발생(發生)하고 있음을 확인(確認)하였다.

• Current Research on Agriculture and Life Sciences
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• 제3권
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• pp.120-128
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• 1985

대구(大邱)를 중심(中心)으로 한 5개(個) 지역(地域)에 재식(裁植)되어 있는 23과(科) 28속(屬) 32종(種)의 관상식물(觀賞植物)에 대(對)하여 진균(眞菌)에 이병(罹病)된 식물(植物)을 조사(調査)한 결과(結果) Uncinula 속균(屬菌) 등(等) 21속(屬)의 병원진균(病原眞菌)에 의(依)하여 26속(屬) 30(種)의 식물(植物)에서 45종(種)의 병(病)이 발생(發生)하고 있었다. 이들 중(中) 우리나라에서 이제까지 보고(報告)되지 않은 미기록병해(未記錄病害)는 Uncinula속균(屬菌), Phyllosticta속균(屬菌), Discosia속균(屬菌), Macrophoma속균(屬菌), Colletotrichum속균(屬菌), Pestalotia속균(屬菌), Phomopsis속균(屬菌), Gloeosporium속균(屬菌), Alternaria속균(屬菌), Fusicoccum속균(屬菌) 및 Sphaceloma속균(屬菌) 등(等)에 의(依)하여 15속(屬) 16종(種)의 식물(植物)에 22종(種)의 병(病)이 발생(發生)하고 있었다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1250-1258
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• 2009

Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RT-PCR analysis of the signaling regulatory genes Coil and NPR1, and defense genes PR-1a and PR-1b, in the tobacco treated with the Bacillus spp. revealed an association with enhancing the systemic resistance of tobacco to TMV. A further analysis of two expansin genes that regulate plant cell growth, NtEXP2 and NtEXP6, also verified a concomitant growth promotion in the roots and leaves of the tobacco responding to the Bacillus spp.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.25-27
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• 2003

Since 1993, a total of 50 problematic plant diseases unrecorded in Korea were surveyed in Gyeongnam province. Totally 34 new host plants to corresponding pathogens investigated in this study were 5 fruit trees, 9 vegetables, 12 ornamental plants, 3 industrial crops, and 5 medicinal plants. Among the newly recorded fruit tree diseases, fruit rot of pomegranate caused by Coniella granati and Rhizopus soft rot of peach caused by Rhizopus nigricans damaged severely showing 65.5% and 82.4% infection rate. Among the vegetable diseases, corynespora leaf spot of pepper caused by Corynespora cassiicola and the crown gall of pepper caused by Agrobacterium tumefaciens, powdery mildew of tomato caused by Oidiopsis taurica were the most severe revealing 47.6%, 84.7%, and 54.5% infection rate in heavily infected fields, respectively. In ornamental plants, collar rot of lily caused by Sclerotium rolfsii, gray mold of primula caused by Botrytis cinerea, soot leaf blight of dendrobium caused by Pseudocercospora dendrobium, sclerotinia rot of obedient plant caused by Sclerotinia sclerotiorum showed 32.7 to 64.8% disease incidence. On three industrial plants such as sword bean, broad bean, and cowpea, eight diseases were firstly found in this study. Among the diseases occurring on broad bean, rust caused by Uromyces viciae-fabae and red spot caused by Botrytis fabae were the major limiting factor for the cultivation of the plant showing over 64% infection rate in fields. In medicinal plants, anthracnose of safflower caused by Collectotrichum acutatum was considered the most severe disease on the plant and followed by collar rot caused by Sclerotium rolfsii.(중략)

• International Journal of Computer Science & Network Security
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• 제22권10호
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• pp.257-261
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• 2022

Diseases of agricultural plants in recent years have spread greatly across the regions of the Kyrgyz Republic and pose a serious threat to the yield of many crops. The consequences of it can greatly affect the food security for an entire country. Due to force majeure, abnormal cases in climatic conditions, the annual incomes of many farmers and agricultural producers can be destroyed locally. Along with this, the rapid detection of plant diseases also remains difficult in many parts of the regions due to the lack of necessary infrastructure. In this case, it is possible to pave the way for the diagnosis of diseases with the help of the latest achievements due to the possibilities of feedback from the farmer - developer in the formation and updating of the database of sick and healthy plants with the help of advances in computer vision, developing on the basis of machine and deep learning. Currently, model training is increasingly used already on publicly available datasets, i.e. it has become popular to build new models already on trained models. The latter is called as transfer training and is developing very quickly. Using a publicly available data set from PlantVillage, which consists of 54,306 or NewPlantVillage with a data volumed with 87,356 images of sick and healthy plant leaves collected under controlled conditions, it is possible to build a deep convolutional neural network to identify 14 types of crops and 26 diseases. At the same time, the trained model can achieve an accuracy of more than 99% on a specially selected test set.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.194-203
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• 2009

$HpaG_{Xooc}$, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the $HpaG_{Xooc}$ protein in Bacillus subtilis, we constructed a recombinant expression vector pM43HF with stronger promoter P43 and signal peptide element nprB. The SDS-PAGE and Western blot analysis demonstrated the expression of the protein $HpaG_{Xooc}$ in B. subtilis. The ELISA analysis determined the optimum condition for $HpaG_{Xooc}$ expression in B. subtilis WBHF. The biological function analysis indicated that the protein $HpaG_{Xooc}$ from B. subtilis WBHF elicits hypersensitive response(HR) and enhances the growth of tobacco. The results of RT-PCR analysis revealed that $HpaG_{Xooc}$ induces expression of the pathogenesis-related genes PR-1a and PR-1b in plant defense response.

• Journal of Ginseng Research
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• 제39권3호
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• pp.213-220
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• 2015

Background: Korean ginseng (Panax ginseng Meyer) is a perennial herb prone to various root diseases, with Phytophthora cactorum being considered one of the most dreaded pathogens. P. cactorum causes foliar blight and root rot. Although chemical pesticides are available for disease control, attention has been shifted to viable, eco-friendly, and cost-effective biological means such as plant growth-promoting rhizobacteria (PGPR) for control of diseases. Methods: Native Bacillus amyloliquefaciens strain HK34 was isolated from wild ginseng and assessed as a biological control agent for ginseng. Leaves from plants treated with HK34 were analyzed for induced systemic resistance (ISR) against P. cactorum in square plate assay. Treated plants were verified for differential expression of defense-related marker genes using quantitative reverse transcription polymerase chain reaction. Results: A total of 78 native rhizosphere bacilli from wild P. ginseng were isolated. One of the root-associated bacteria identified as B. amyloliquefaciens strain HK34 effectively induced resistance against P. cactorum when applied as soil drench once (99.1% disease control) and as a priming treatment two times in the early stages (83.9% disease control). A similar result was observed in the leaf samples of plants under field conditions, where the percentage of disease control was 85.6%. Significant upregulation of the genes PgPR10, PgPR5, and PgCAT in the leaves of plants treated with HK34 was observed against P. cactorum compared with untreated controls and only pathogen-treated plants. Conclusion: The results of this study indicate HK34 as a potential biocontrol agent eliciting ISR in ginseng against P. cactorum.

• Current Research on Agriculture and Life Sciences
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• 제9권
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• pp.115-125
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• 1991

우리나라와 일본(日本)에서 야생 또는 재배되고 있는 약용식물(藥用植物)의 바이러스병을 조사한 결과 33종의 식물이 자연상태에서 오이 모자이크 바이러스(CMV)에 감염되어 있음을 알았다. 이들 중에서 개맨드라미(Celosia argenteia)와 쇠비름(Portulaca oleracea)의 모자이크병(가칭(假稱)), 쥐방울덩굴(Aristolochia debilis)과 번행초(Tetragonia expansa)의 괴저(壞疽)모자이크병(가칭(假稱)), basella(Basella rubra)윤문병(輪紋病)(가칭(假稱)), 석결명(Cassia torosa)과 시호(Bupleurum falcatum), 당귀(Angelica acutiloba), 구릿대(A. keiskei), 회향(Foeniculum vulgare), peucedanum(Peucedanum japanicum)의 반문병(斑紋病)(가칭(假稱))등 11종의 바이러스병명(病名)을 새로히 명명(命名)하였다.

• Journal of Plant Biotechnology
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• 제32권4호
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• pp.233-241
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• 2005

Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.

• The Plant Pathology Journal
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• 제25권4호
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• pp.328-332
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• 2009

Diseases caused by Pepper yellow leaf curl virus infection is considered to be emerging plant diseases in Indonesia in the last five years. One key factor for disease management is the availability of accurate detection of the virus in plants. Polyclonal antibody for Pepper yellow leaf curl Indonesia virus-Bogor (PYLCIV-Bgr) was produced for detection of the virus using I-ELISA and DIBA methods. The antibody was able to detect PYLCIV-Bgr from infected plants up to dilution 1/16,384 and cross reaction was not observed with Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), and Chilli veinal mottle virus (ChiVMV). Positive reaction was readily detected in membrane containing Begomovirus samples from Yogyakarta (Kaliurang and Kulonprogo) and West Java (Bogor and Segunung). Infection of PYLCIV-Bgr in chillipepper, tomato, and Ageratum conyzoides was also confirmed using polyclonal antibody for PYLCIV-Bgr in DIBA. Polyclonal antibody for PYLCIV-Bgr is suggested to be included in disease management approach due to its good detection level.