• 제목/요약/키워드: Disc diffusion method

검색결과 224건 처리시간 0.033초

Antimicrobial Activity of Methyl Gallate isolated from the Leaves of Glochidion superbum Against Hospital Isolates of Methicillin Resistant Staphylococcus aureus

  • Ahmed, Mohammed Dahiru;Taher, Muhammad;Maimusa, Alhaji Hamusu;Rezali, Mohamad Fazlin;Mahmud, Mohammed Imad Al-deen Mustafa
    • Natural Product Sciences
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    • 제23권1호
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    • pp.5-8
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    • 2017
  • An antimicrobial compound has been isolated from the leaves of Glochidion superbum. The compound was determined as methyl 3, 4, 5-trihydroxybenzoate (methyl gallate), based on ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) analysis. The isolated compound exhibited potent antimicrobial activity against three clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) by qualitative agar disc diffusion method and quantitative broth dilution method. Agar disc diffusion was done in a dose-dependent manner for each bacterial isolate at disc potencies of 25, 50, 100, and $150{\mu}g/disc$. The zones of inhibition were on average equal to 12.27, 14.20, 15.43, and 24.17 mm respectively. The inhibition zones were compared with that of vancomycin disc at $30{\mu}g$ as a reference standard. The MIC and MBC values were $50{\mu}g/ml$ and $100{\mu}g/ml$ respectively. The results of anti MRSA activity were analyzed using one-way ANOVA with Turkey's HSD and Duncan test. In conclusion, methyl gallate which was isolated from G. superbum showed the inhibition activity against methicillin resistant S. aureus.

Evaluation of the Antibacterial Effects of Phellinus baumii Extract on Methicillin-Resistant Staphylococcus aureus by Using Broth Microdilution Based on a Colorimetric Method

  • Shin, Kyeong Seob;Jo, Mi Young;Hong, Seung Bok
    • 대한의생명과학회지
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    • 제22권4호
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    • pp.167-173
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    • 2016
  • The broth microdilution technique used to measure the minimal inhibitory concentration (MIC) of natural compounds against bacteria is problematic: it is difficult to visualize bacterial growth due to the color of the natural compound. Therefore, the use of a colorimetric method with a redox indicator by broth microdilution can simplify it and increase its objectivity. This study evaluated the usefulness of the colorimetric method in measuring the MIC of Phellinus baumii against methicillin-resistant Staphylococcus aureus (MRSA). The inhibition in disc diffusion method was observed from $8,192{\mu}g/mL$ P. baumii in all 10 MRSA isolates examined; however, the MIC ranges of the 10 MRSA isolates was $512{\sim}2,048{\mu}g/mL$ by broth microdilution using a colorimetric method; with the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) indicator. In addition, the MIC of P. baumii by broth microdilution using MTT as indicator yielded excellent results. However, the 2, 3, 5-triphenyltetrazolium chloride (TTC) results could not be determined due to the color of the TTC indicator. The MICs of four antibiotics against MRSA using MTT or TTC were equal to those determined by visual interpretation. In conclusion, to evaluate the antibacterial effects of a natural compound, the broth microdilution technique is considered to be better than the disc diffusion method. Moreover, to resolve the problems caused by the colors of natural compounds, a colorimetric method such as that using MTT may be very valuable.

Screening of some Bangladeshi medicinal plants for in vitro antibacterial activity

  • Uddin, Shaikh Jamal;Rouf, Razina;Shilpi, Jamil Ahmed;Alamgir, Mohammad;Nahar, Lutfun;Sarker, Satyajit Dey
    • Advances in Traditional Medicine
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    • 제8권3호
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    • pp.316-321
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    • 2008
  • A total of 33 extracts representing 26 plant species belonging to 24 families were collected from different regions of Bangladesh, and screened for their in vitro antibacterial activity against several pathogenic Gram-positive and Gram-negative bacterial strains using the conventional disc diffusion method. The most potent activity was exhibited by the extracts of Aegiceras corniculatum, Alocasia fornicata, Ceriops decandra, Cuscuta reflexa, Lasia spinosa, Lantana camara, Pandanus foetidus and Xylocarpus granatum. The extracts of Abtilon indicum, Derris trifoliata, Dendrophthoe falcat, Ruellia tuberosa and X. moluccensis did not show any antibacterial properties at test concentrations.

Artemisia princeps var. orientalis 수용성 추출물의 항균효과 (Antimicrobial Activity of Water-soluble Extract from Artemisia princeps var. orientalis)

  • 조화영;윤성용;박정진;윤경원;박종문
    • KSBB Journal
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    • 제21권2호
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    • pp.129-132
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    • 2006
  • Artemisia princes var. orientalis의 수용성 추출물이 식품, 화장품, 의약품 등의 항균제로 이용될 수 있는지에 대해 연구 하였다. 순수한 물을 이용한 추출물에서는 항균성을 찾기 힘들었고, 쑥의 줄기와 잎 부분에서 메탄올로 추출한 후 물로 추출한 물이 Staphylococcus aureus에 대해서 항균성을 크게 나타냈으며, 잎이 줄기 부분보다 더 큰 항균성을 나타냄을 확인했다. 항균성을 갖는 물질을 밝혀내기 위해 쑥의 주된 항균 성분이라 알려진 caffeic acid와 비교 실험을 하였다. UV 특성곡선에서 caffeic acid와 유사한 phenolic compound의 profiling은 확인할 수 있었으나 caffeic acid의 disc diffusion method를 이용한 실험에서는 항균능력을 나타내지 않았다. 물질규명을 위해 추출물을 세 부분으로 분획 하였고, 이 중에서 Staphylococcus aureus에 대해 항균성은 UK 2에서 크게 나타났다. 본 연구는 Artemisia princeps var. orientalis 의 메탄올 후 물 추출물이 항균제로써의 가능성을 알아봄으로써 식품 또는 화장품의 보존료나 항균제의 이용성이 있음을 충분히 확인하였다.

오레가노, 육계, 편백 및 황금의 초임계 유체 추출물의 항균 활성 (Antimicrobial Activity of Extracted by Supercritical Fluid from Origanum vulgare, Cinnamomum cassia, Chamaecyparis obtusa and Scutellariae baicalensis)

  • 김우진;조준영;최창숙;윤기선;이원규;유연우
    • KSBB Journal
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    • 제23권2호
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    • pp.147-152
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    • 2008
  • 다양한 기능성을 갖는 식물체를 대상으로 새로운 천연 식품보존제 및 방부제 개발의 일환으로 다양한 방법으로 추출한 항균환성 물질을 몇 종의 병원성균과 식중독균, 효모, 곰팡이 등 10개 균주에 대하여 항균활성 검색을 하였다. 각 추출방법에 따른 추출 수율을 보면 편백과 육계 초임계 추출물의 수율이 가장 높았지만, 오레가노와 황금의 경우에는 초임계 추출물의 수율이 가장 낮았다. 그러나 항균활성 실험에서 disc-diffusion method 및 최소억제농도 (MIC) 실험 결과 유기용매 추출 및 열수 추출보다 초임계 추출의 경우가 항균활성이 모든 시료에서 높은 것으로 나타났다. Disc-diffusion method에서 육계 초임계 추출물의 경우 에탄올 추출물보다 항균활성이 두 배정도 높게 나타났고, 10개 균주에 대해 모두 항균활성을 나타냈다. 황금 초임계 추출물은 L. monocytogenes에 강한 항균활성을 보였고, 오레가노와 편백의 초임계 추출물은 S. typhimurium에 강한 항균활성을 보였다. 최소억제농도 (MIC)에서는 편백 초임계 추출물이 다른 초임계 추출물에 비해 강한 항균활성을 보였으며, 그 다음으로 육계, 황금, 오레가노 초임계 추출물의 순으로 항균활성을 나타내었다. 따라서 천연물을 이용한 항균제 개발에 있어 초임계 추출방법이 항균력이 높은 기능성 성분을 추출해 내는데 가장 효과적인 추출방법인 것으로 판단된다.

새송이버섯 추출물이 구강세균에 작용하는 항균효과 (The antibacterial effect of Pleurotus eryngii extracts on oral bacteria)

  • 전인영;유은지;유상철;이지연;정상희;오태진
    • 한국치위생학회지
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    • 제18권1호
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    • pp.9-18
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    • 2018
  • Objectives: Pleurotus eryngii is used both for edible and medicinal purposes, and has a physiological activity. The purpose of this study is to investigate the antibacterial effect of Pleurotus eryngii against six oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Methods: The antibacterial activities of various extracts of Pleurotus eryngii were examined by disc diffusion assay and minimum inhibitory concentration (MIC). The disc diffusion assay was performed by putting a paper disc soaked in extracts on plates inoculated bacterial cultures. The MIC of these extracts was determined by using a broth microdilution assay at a concentration ranging between 0.03 mg/ml to 15.00 mg/ml. The growth inhibition effect of extracts was measured at 600 nm for 24 hrs. Results: The antibacterial activity was confirmed against all six tested bacteria at Pleurotus eryngii ethyl acetate extract by the disc diffusion method. Acetone extract showed the antibacterial activity only against 4 strains containing Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, and Actinomyces viscosus. In ethanol extract, no activity was observed against other strains except Staphylococcus aureus. MIC values of ethyl acetate extract were the same, 7.50 mg/ml in all tested bacteria. Conclusions: Pleurotus eryngii exhibited the antibacterial activity against oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Thus, Pleurotus eryngii may be considered as a natural antibacterial agent for treatment of dental diseases.

대나무 기름의 항균효과 (Antimicrobial Activity of Bamboo(Phyllostachys bambusoides) Essential Oil)

  • 이숙경
    • 한국식품위생안전성학회지
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    • 제15권1호
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    • pp.55-59
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    • 2000
  • Bamboo oil의 천연 보존료로서의 가치를 평가하기 위해 합성 보존료인 BHA와 이미 수입되어 사용되고 있는 천연보존료인 tea tree oil을 비교하기 위하여 disc diffusion법과 broth dilution 법으로 S. aureus와 E. coli대한 항균력을 검토한 결과를 요약하면 다음과 같다. 1. Disc diffusion법으로 S. aureus에 대한 항균력을 조사한 결과 BHA>tea tree oil>bamboo oil 순이었고 E. coli에 대한 항균력은 bamboo oil이 가장 강하게 나타났으며 다음은 BHA>tea tree oil순으로 나타났다. 2. Broth dilution법으로 S. aureus와 E. coli모든 시료 6.0$\mu$l/ ml 이상에서 모두 측정되지 않아 모든 시료에서 동일하게 최소억제농도가 6.0$\mu$l/ ml 이상 필요한 것으로 나타났다. 3. Bamboo oil의 이용 개발 시 경제적 조건은 동일한 항균력을 기대할 때 tea tree oil에 비하여 약 20%정도 저렴하여 경제성이 충분히 있는 것으로 나타났다.

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Antimicrobial activity and toxicity of Quisqualis indica

  • Jahan, Fatima N.;Rahman, Mohammad S.;Hossain, Mahboob;Rashid, Mohammad A.
    • Advances in Traditional Medicine
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    • 제8권1호
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    • pp.53-58
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    • 2008
  • The plant Quisqualis indica (Compositae) has ehnopharmcological reputation of being used as a healing agent in Bangladesh. In this study, preliminary screenings were conducted to look at the antimicrobial susceptibility and cytotoxicity of the plant extract. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract ranged from 8 - 15 mm, 8 - 18 mm, 12 - 20 mm and 10 - 16 mm, respectively at a concentration of 400 ${\mu}g$/disc. All the extractives were also subjected to brine shrimp lethality bioassay for primary cytotoxicity evaluation. Here, the carbon tetrachloride soluble materials demonstrated the highest cytotoxicity with $LC_{50}$ of 0.826 ${\mu}g$/ml, while n-hexane, chloroform and aqueous soluble partitionates of the methanolic extract revealed the $LC_{50}$ of 1.254, 3.866 and 5.366 ${\mu}g$/ml, respectively. This is the first report of the antimicrobial activity and cytotoxicity Q. indica.

SeO2의 메티실린-내성 황색포도상구균에 대한 생육 억제 효과 (Inhibitory effect of SeO2 on cell growth of methicillin-resistant Staphylococcus aureus)

  • 한영환
    • 미생물학회지
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    • 제51권4호
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    • pp.444-447
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    • 2015
  • 본 연구는 $SeO_2$의 메티실린-내성 S. aureus에 대한 항세균활성을 규명하고자 수행하였다. Disc diffusion method를 이용하여 $SeO_2$의 항세균 활성을 측정한 결과, 그람 양성 세균이 그람 음성 세균과 비교시 우수하였다. 사용한 그람 양성 세균 중 Streptococcus, Staphylococcs 속 세균이 Bacillus 속 간균과 비교시 더 우수하였다. 사용한 모든 MRSA에 항균활성이 나타났다. 항생제의 생육저지환을 측정한 결과, 사용한 모든 항생제에 대해 MRSA의 항균활성이 작게 나타났다. $200-500{\mu}g/disc$ 범위의 $SeO_2$ 적용시 S. aureus 및 S. aureus CCARM (MRSA)에 대한 생육저지환의 직경은 각각 20-32.7 mm 및 13.5-17.9 mm이었다. $SeO_2$의 MRSA에 대한 최소생육억제농도는 $40{\mu}g/ml$이었다. 액체배지에 0.5% 및 1%의 $SeO_2$를 첨가한 결과, MRSA의 생육이 억제 되었다. 본 $SeO_2$의 항균활성 실험 결과는 추후, $SeO_2$의 항균활성 기작의 규명, 병원성 세균 및 항생제-내성 미생물에 적용될 수 있다고 판단된다.

Antimicrobial activity of Mongolian medicinal plants

  • Gonchig, Enkhmaa;Erdenebat, Sarnaizul;Togtoo, Ouyntsetseg;Bataa, Sukhkhuu;Gendaram, Odontuya;Kim, Young-Sup;Ryu, Shi-Yong
    • Natural Product Sciences
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    • 제14권1호
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    • pp.32-36
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    • 2008
  • The antimicrobial activity of seventy five ethanol extracts obtained from 67 different kinds of plant species of the Mongolian flora were evaluated by means of the disc diffusion method against five species of microorganisms, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Micrococcus luteus and Pseudomonas aeruginosa. Among the plant extracts examined, 34 kinds of extracts demonstrated significant antibacterial activity against one or more species of microorganisms, respectively. Especially, the root extract of Paeonia anomala, the whole herb extract of Myricaria alopecuroides, the whole herb extract of comarum zalesovianum, the whole herb extract of Agrimonia pilosa and some other plant extracts demonstrated a particularly potent antimicrobial activity. The ethylacetate fractions obtained from the whole herb extract of Myricaria alopecuroides and from those of Sedum aizoon, Paeonia anomala, Sedum hybridum and Dasiphora fruticosa exhibited a particularly potent antibacterial activity especially against Staphylococcus aureus and Micrococcus luteus.