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http://dx.doi.org/10.20307/nps.2017.23.1.5

Antimicrobial Activity of Methyl Gallate isolated from the Leaves of Glochidion superbum Against Hospital Isolates of Methicillin Resistant Staphylococcus aureus  

Ahmed, Mohammed Dahiru (Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia)
Taher, Muhammad (Deparment of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia)
Maimusa, Alhaji Hamusu (Vector Control Unit, Faculty of Biological Sciences)
Rezali, Mohamad Fazlin (SIRIM Berhad, National Metrology Laboratory)
Mahmud, Mohammed Imad Al-deen Mustafa (Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia)
Publication Information
Natural Product Sciences / v.23, no.1, 2017 , pp. 5-8 More about this Journal
Abstract
An antimicrobial compound has been isolated from the leaves of Glochidion superbum. The compound was determined as methyl 3, 4, 5-trihydroxybenzoate (methyl gallate), based on ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) analysis. The isolated compound exhibited potent antimicrobial activity against three clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) by qualitative agar disc diffusion method and quantitative broth dilution method. Agar disc diffusion was done in a dose-dependent manner for each bacterial isolate at disc potencies of 25, 50, 100, and $150{\mu}g/disc$. The zones of inhibition were on average equal to 12.27, 14.20, 15.43, and 24.17 mm respectively. The inhibition zones were compared with that of vancomycin disc at $30{\mu}g$ as a reference standard. The MIC and MBC values were $50{\mu}g/ml$ and $100{\mu}g/ml$ respectively. The results of anti MRSA activity were analyzed using one-way ANOVA with Turkey's HSD and Duncan test. In conclusion, methyl gallate which was isolated from G. superbum showed the inhibition activity against methicillin resistant S. aureus.
Keywords
Glochidion superbum; antimicrobial activity; isolation; methyl gallat;
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