DOI QR코드

DOI QR Code

Antimicrobial Activity of Methyl Gallate isolated from the Leaves of Glochidion superbum Against Hospital Isolates of Methicillin Resistant Staphylococcus aureus

  • Received : 2016.06.29
  • Accepted : 2016.10.29
  • Published : 2017.03.31

Abstract

An antimicrobial compound has been isolated from the leaves of Glochidion superbum. The compound was determined as methyl 3, 4, 5-trihydroxybenzoate (methyl gallate), based on ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) analysis. The isolated compound exhibited potent antimicrobial activity against three clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) by qualitative agar disc diffusion method and quantitative broth dilution method. Agar disc diffusion was done in a dose-dependent manner for each bacterial isolate at disc potencies of 25, 50, 100, and $150{\mu}g/disc$. The zones of inhibition were on average equal to 12.27, 14.20, 15.43, and 24.17 mm respectively. The inhibition zones were compared with that of vancomycin disc at $30{\mu}g$ as a reference standard. The MIC and MBC values were $50{\mu}g/ml$ and $100{\mu}g/ml$ respectively. The results of anti MRSA activity were analyzed using one-way ANOVA with Turkey's HSD and Duncan test. In conclusion, methyl gallate which was isolated from G. superbum showed the inhibition activity against methicillin resistant S. aureus.

Keywords

References

  1. Sandhya, S.; Chaintanya, R. S. N. A. K. K.; Vinod, K. R.; Rao, K. N. V.; Banji, D.; Sudhakar, K.; &Swetha, R., Arch. Appl. Sci. Res. 2010, 2, 309-322.
  2. Otsuka, H.; Hirata, E., Shinzato, T.; Takeda, Y. Phytochemistry 2003, 62, 763-768. https://doi.org/10.1016/S0031-9422(02)00614-3
  3. Joshi, A. R.; Joshi, K Ethnobotanical Leaflets 2007, 15, 235-246.
  4. RIZK, A. F. M. Botanical J. Linnean Society 1987, 94, 293-326. https://doi.org/10.1111/j.1095-8339.1987.tb01052.x
  5. Bailey, A. E.; Asplund, R. O.; and Ali, M. S. J. Natrod. 1986, 9, 1149-1150.
  6. Choi, J. G.; Mun, S. H.; Chahar, H. S.; Bharaj, P.; Kang, O. H.; Kim, S. G.; Kwon, D. Y.PLOS One 2014, 9, 1149-1150.
  7. Wang, K. J; ang, C. R.; Zhang, Y. J. Food Chemistry 2007, 101 365-371. https://doi.org/10.1016/j.foodchem.2006.01.044
  8. Saxena, G.; McCutcheon, A. R.; Farmer, S.; Towers, G. H.; Hancock, R. E. J. Ethnopharmacol. 1994, 42, 95-99. https://doi.org/10.1016/0378-8741(94)90102-3
  9. Ekaprasada, M. T.; NurdinH.; Ibrahim, S.; Dachriyanus, D. Int. J. Advan. Sci. EngineerInforma. Technology 2015, 5, 280-282.
  10. Karpanoja, P.; Nissinen, A.; Huovinen, P.; Sarkkinen, H.; Finnish Study Group for Antimicrobial Resistance. J. Antimicrob. Chemother. 2004, 53, 660-663. https://doi.org/10.1093/jac/dkh134
  11. Raeisi, M.; Tajik, H.; Razavi, R. S.; Maham, M.; Moradi, M.; Hajimohammadi, B.; Mehdizadeh, T. Iran. J. Microbiol. 2012, 4, 30-34.
  12. Rahman, N. A.; Muliawan,. H. S.; Rashid, N. N.; Muhamad, M.;Yusof, R.N. Dengue Bull. 2006, 30, 260-269.
  13. Ekaprasada, M. T.; Nurdin, H.; Ibrahim, S.; Dachriyanus, D. Indo. J. Chem. 2010, 9, 457-460.
  14. Tan, Y. P.; Chan, E. W. C.; Lim, C. S. Y. Chiang Mai J. Sci. 2015, 42, 650-656.

Cited by

  1. A New Ellagic Acid From the Leaves and Twigs of Irvingia malayana vol.14, pp.5, 2017, https://doi.org/10.1177/1934578x19848164
  2. Evaluation of the pharmacokinetic-pharmacodynamic integration of marbofloxacin in combination with methyl gallate against Salmonella Typhimurium in rats vol.15, pp.6, 2017, https://doi.org/10.1371/journal.pone.0234211
  3. The antimicrobial and antioxidant property, GC-MS analysis of non-edible oil-seed cakes of neem, madhuca, and simarouba vol.45, pp.1, 2017, https://doi.org/10.1186/s42269-021-00498-x