• Reproductive and Developmental Biology
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• 제35권1호
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• pp.77-83
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• 2011

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 하계종합학술대회 논문집(1)
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• pp.281-284
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• 2004

This paper presents the design and implementation of a QoS based enhanced collaboration system in MDO. This is an efficient distributed communication tool between designers. It supports text communication, audio/video communication, file transfer and XML data sending/receiving. Specially, this system supports a dynamic QoS self-adaptation by using the improved direct adjustment algorithm (DAA+). The original direct adjustment algorithm adjusts the transmission rate according to the congestion level of the network, based on the end to end real time transport protocol (RTP), and controls the transmission rate by using the information of loss ratio in real time transport control protocol (RTCP). But the direct adjustment algorithm does not consider when the RTCP packets are lost. We suggest an improved direct adjustment algorithm to solve this problem. We apply our improved direct adjustment algorithm to our of QoS (Quality of Service) [1] based collaboration system and show the improved performance of transmission rate and loss ratio.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
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• pp.158-158
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• 2011

A wide range of techniques for the direct-printing of functional materials have been developed for the fabrication of micro- and nanoscale structures and devices. Here we report a new direct patterning method, liquid bridge-mediated nanotransfer molding (LB-nTM), for the formation of two- or three-dimensional structures with feature sized as small as tens of nanometers over large areas up to 4". LB-nTM is based on the direct transfer of various materials from a mold to a substrate via a liquid bridge between them. The LB-nTM method was applied to the preparation of organic nanowire FETs on flexible substrates.

• 태양에너지
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• 제18권4호
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• pp.33-38
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• 1998

This paper reports the experimental results concerning the thermal performance of a freeze-protected direct solar water heating system. The direct system tested has been manufactured to prevent any breakage during harsh wintry conditions by specially designed riser and downcomer headers capable of relieving extra pressure due to freezing of water. The direct system tested has shown 10% improvement in its efficiency over that of a indirect type system using antifreeze as the heat transfer medium. It is felt that any direct system of its kind could galvanize the whole solar industry if further improvements are made to ensure its durability.

• Steel and Composite Structures
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• 제11권2호
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• pp.169-181
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• 2011

The behavior of connections between open sandwich slabs and double steel skin composite walls in steel plate-concrete(SC) structure is investigated by a series of experimental programs to identify the roles of components in the transfer of forces. Such connections are supposed to transfer shear by the action of friction on the interface between the steel surface and the concrete surface, as well as the shear resistance of the bottom steel plate attached to the wall. Experimental observation showed that shear transfer in slabs subjected to shear in short spans is explained by direct force transfer via diagonal struts and indirect force transfer via truss actions. Shear resistance at the interface is enhanced by the shear capacity of the shear plate as well as friction caused by the compressive force along the wall plate. Shear friction resistance along the wall plate was deduced from experimental observation. Finally, the appropriate design strength of the connection is proposed for a practical design purpose.

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2409-2413
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• 2007

Time resolved phosphorescence of Dibromobenzophenone (DBBP) choleic acid crystal was observed at 4.2 K as functions of excitation energy and delay time. The experimental results reveal that the energy transfer efficiency is dependent on the excitation energy, i.e. the density of acceptors sites. As the excitation energy or delay time increases, the resonance phosphorescence does not broaden and shift gradually, rather a broad luminescence band develops about 290 cm?1 to lower energy of the resonance phosphorescence. The observation implies that energy transfer from high to low energy sites in this system is controlled by emission of phonons or vibrons. The data of time resolved experiments were analyzed in terms of a mechanism involving direct donor-acceptor excitation transport by exchange coupling. It was concluded that an isotropic twodimensional exchange interaction topology is consistent with energy transfer in this system.

• Rapid Communication in Photoscience
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• 제4권3호
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• pp.59-62
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• 2015

We investigated photo-induced electron transfer (PET) in a dye-labeled peptide, fluorophore-Ala-Gly-Gln-Tyr, employing time-resolved fluorescence. As an effort to develop new functional dyes, we studied an acriflavine derivative for the electron-acceptor in the excited state from tyrosine, an electrondonor in the ground-state. The pH dependence of the fluorescence lifetime of the model peptide indicates that electron transfer between the excited dye and tyrosine occurs when the tyrosine is deprotonated. The proton-coupled electron transfer appears to be sequential rather than concerted. We also report direct time measurements on the end-to-end loop formation processes of the peptide in water.

• 한국분무공학회지
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• 제27권3호
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• pp.126-133
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• 2022

The objective of this is to experimentally investigate the effect of mixed jet on the oxygen transfer characteristics with the primary nozzle area ratio of an annular nozzle ejector for the application of a microbial fuel cell. A direct visualization method with a high speed camera system was used to capture the horizontal mixed jet images, and a binarization technique was used to analyze the images. The clean water unsteady state technique was used for the oxygen transfer measurement. The air-water mixed jet discharging into a water tank behaved similar to a buoyancy or horizontal jet with the primary nozzle area ratio. It was found that an optimum primary nozzle area ratio was observed where the oxygen transfer performance reached its maximum value due to the decrease of air volume fraction and the increase of jet length and air bubble dispersion.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.201-201
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• 2004

In this study we examined whether vitrified Hanwoo (Korean cattle) IVM/IVF/IVC blastocysts can survive in vitro/in vivo by a quick one-step dilution method and these embryos result in live births. Blastocysts produced in vitro were vitrified by serial exposure to glycerol (G) and/or ethylene glycol (EG) mixtures of 10% (v/v) G for 5 min, 10% G plus 20% EG (v/v) for 5 min, and 25% G plus 25% EG (v/v) for 30 see. (omitted)

• 한국해양공학회지
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• 제13권3B호
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• pp.91-98
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• 1999

In order to reject the steady-state tracking error, it is common to introduce integral compensators in servosystems for constant reference signals. However, if the mathematical model of the plant is exact and no disturbance input exists, the integral compensation is not necessary. From this point of view, a two-degree-of-freedom(2DOF) servosystem has been proposed, in which the integral compensation is effective only when there is a modeling error or a disturbance input. The present paper considers a synthesis problems of this 32DOF servosystem with direct transfer term in the system representation. And, a method how we may obtain a gain such that desirable transient response is achieved, is proposed in the presence of the modelling error and disturbance input.