Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.201-201
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- 2004
Calving Production from Hanwoo (Korean Cattle) IVM/IVF/IVC Blastocysts: Direct Transfer of Vitrified and Quick One-Step Diluted Hanwoo Blastocysts
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Kim, Deok-Im (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Tae, Jin-Cheol (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Kim, Deok-Im (Hanwoo Improvement Center, NLC) ;
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Lee, Won-Don (Maria Infertility Hospita) ;
-
Park, Sepill
(Maria Infertility Hospital Medical Institute/Maria Biotec)
;
- Lim, Jin-Ho (Maria Infertility Hospital)
- Published : 2004.06.01
Abstract
In this study we examined whether vitrified Hanwoo (Korean cattle) IVM/IVF/IVC blastocysts can survive in vitro/in vivo by a quick one-step dilution method and these embryos result in live births. Blastocysts produced in vitro were vitrified by serial exposure to glycerol (G) and/or ethylene glycol (EG) mixtures of 10% (v/v) G for 5 min, 10% G plus 20% EG (v/v) for 5 min, and 25% G plus 25% EG (v/v) for 30 see. (omitted)