• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1239-1248
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• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Journal of Biosystems Engineering
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• v.30 no.1 s.108
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• pp.45-55
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• 2005

Knowledge of the relationship between crop yield and yield-limiting factors is essential for precision farming. However, developing this knowledge is not easy because these yield-limiting factors are interrelated and affect crop yield in different ways. In this study, data for grain yield and yield-limiting factors, including crop chlorophyll content, soil chemical properties, and topography were collected for a small (0.3 ha) rice paddy field in Korea and a large (36 ha) upland corn field in the USA, and relationships were investigated with path analysis. Using this approach, the effects of limiting factors on crop yield could be separated into direct effects and indirect effects acting through other factors. Path analysis provided more insight into these complex relationships than did simple correlation or multiple linear regression analysis. Results of correlation analysis for the rice paddy field showed that EC, Ca, and $SiO_2$ had significant (P<0.1) correlations with rice yield, while pH, Ca, Mg, Na, $SiO_2,\;and\;P_2O_5$ had significant correlations with the SPAD chlorophyll reading. Path analysis provided additional information about the importance and contribution paths of soil variables to rice yield and growth. Ca had the highest direct effect (0.52) and indirect effect via Mg (-0.37) on rice yield. The indirect effect of Mg through Ca (0.51) was higher than the direct effect (-0.38). Path analysis also enabled more appropriate selection of important factors limiting crop yield by considering cause-and-effect relationships among predictor and response variables. For example, although pH showed a positive correlation (r=0.35) with SPAD readings, the correlation was mainly due to the indirect positive effects acting through Mg and $SiO_2$, while pH not only showed negative direct effects, but also negatively impacted indirect effects of other variables on SPAD readings. For the large upland Missouri corn field, two topographic factors, elevation and slope, had significant (P<0.1) direct effects on yield and highly significant (P<0.01) correlations with other limiting factors. Based on the correlation analysis alone, P and K were determined to be nutrients that would increase corn yield for this field. With the help of path analysis, however, increases in Mg could also be expected to increase corn yield in this case. In general, path analysis results were consistent with published optimum ranges of nutrients for rice and com production. We conclude that path analysis can be a useful tool to investigate interrelationships between crop yield and yield limiting factors on a site-specific basis.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.2 no.1
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• pp.132-177
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• 1974

Toe public library may perform a social good only when its objectives are adequate to the community's needs. The present purpose of the public library in the Korean Library Act has no direct concern for our social needs from its very beginning because it was not created in response to our social needs but transplanted from American principles. It is, therefore. difficult to expect socially useful output of our public library programs under the present purpose which may be inadequate to our social tradition and needs. This study purposes to examine validity of the present purpose of the public library in the Korean Library Act, in the light of our community needs and our own library tradition. The methods employed to achieve the purpose of the study are (1) to survey needs of the library frequenters through questionnaire, and interviews with the public librarians in Seoul, (2) to gather the statistical data relevant to, and supporting, the hypothesis, and (3) to compare our social background with that of the United States of which the American purpose, a model for our present purpose, came out. The conclusion is as follows: A. The idea to induce people to pull themselves upward by providing for all the members of the community access to the world's best books shelved in the public library should be abandoned. The reasons are (1) that the idea is alien in our public library tradition, (2) that little demand of the community goes with the idea, and (3) that reading outside the library has come into wide practice, thanks to recent increase in individual income and that in publications. B. That the public library maintains fiction and other recreational reading materials is meaningless in the light of the community needs. These are the two explanations supporting the thesis. (1) The "uplift" theory has proved inoperative and people apparently do not progress from, light fiction to more respectable fare. (2) The conviction that fiction and other recreational reading materials keep the middling classes from the "vicious" entertainments maintaining order in the community by giving them a harmless source of recreation has lost its significance as the modern society provides a number of choices in recreation: television is an obvious example. C. The nature of the informational needs of the community has radically changed, so radically as to require substantial changes in the outlook, collections, and services of the public library, which is :slow in adopting itself to the new social surroundings in Korea. D. 92.2 per Cent of the present frequenters of the public library are high school and college students. Since the library is to meet the existing community needs it should turn its attention to the student group, and develop the means to serve it better, not the "theoretical group of specialists who do not come to "the public library. E. In revision of the purpose of the public library, priority of each objective should be given. The priorities in the last analysis are research and information. culture, recreation in that order.

• Journal of Digital Contents Society
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• v.18 no.1
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• pp.63-70
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• 2017

Recently, as user feedback such as social reading become active, demand has been increased on e-book contents making which is based on user feedback. However, to reflect the user feedback onto the e-book, direct coding is required, which was difficult to the author who was not good at programming. To resolve this problem, Block assembly style feedback editor system, using Blockly was developed. This editor enables to reflect the user feedback by area allocation, component allocation, block editing, and code generating insertion, contrary to the existing way of programming realization in which direct coding was required for input, processing and output separately. This system was developed by using HTML. Javascript, PHP, and Codeigniter. Block editing is enabled to do provision and assembly of blocks by Blockly. The function of code generation & insertion allows to insert the Library function code. Through this system, the general users who are not capable of coding also can reflect feedback without doing actual coding.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.5
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• pp.124-133
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• 2014

This study aims to track pedestrian behavior in urban linear tourist sites and to analyse their behavioral characteristics. For these purposes, this study has adopted the direct observation, a behavioral tracking methodology, and selected Chenggyecheon in Seoul as the study site, a representative site of urban regeneration. The main results of this study can be summarized as follows: the period of stay and movement distance of pedestrians in the study area are both short and most pedestrian activities, apart from walking, are of a passive nature, such as pitching pennies, reading signs, resting and the like. Spatial points of Chenggyecheon at which pedestrians have exhibited these behaviors during their stay are summarized as 30 points and classified into 3 types: landmarks, bridges and nodes. Further research that combines several tracking methods in terms of urban pedestrian behavior is needed.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.18 no.1
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• pp.58-65
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• 2007

Objectives : Motivational factor is a unique contributor to the typically poor academic performance of children with ADHD. However, few study has directly intervened learning motivation in children with ADHD. We conducted this study to explore the direct effects of the learning motivation improvement program applied to children with ADHD. Method The program was designed in order to increase an interest-inducing educational intervention, an academic skills integration, a basic learning activity (reading, writing, and math), and children's self-esteem. We conducted the program twice a week (total 10 sessions) and assessed learning motivation, teaming attitude, self-esteem, academic performance, and problem behaviors of participating children. Results : After the program, teachers reported improvement in teaming motivation. In addition, parents notified sisnificant reduction of problem behaviors. Children reported improvement in a few domains of teaming motivation and learning attitude. Conclusion : While loaming motivation is regarded as an important factor in education, there have been few studies considering this issue in both educational and psychiatric fields. The teaming motivation improvement would be needed in both field in order to reduce the deficits in academic performance in children with ADHD.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.24 no.4
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• pp.471-477
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• 2014

Objectives: This study was conducted to evaluate area exposure of airborne dust and organic gas during entrance and shipment of chicken in poultry farms Methods: Total dust was measured with NIOSH method 0500. The volume of distributed dust and organic gas(Ammonia, TVOC, Hydrogen Sulfide) were measured using direct reading instrument. Results: The range of concentrations of total dust in area sample was $0.07{\sim}4.91mg/m^3$ during the entrance of chicks and $4.37{\sim}9.4mg/m^3$ during shipment respectively. Concentration of ammonia reached approximately 9 ppm during shipment. There was a difference of concentration of total dust in the area sample between shipment and entrance tasks. Conclusions: It was found that the development of a special intervention program by type of task should be considered for reducing respiratory health effects among poultry farmers.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.20 no.4
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• pp.248-255
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• 2010

Airborne nanoparticle concentrations in three metrics (particle surface area concentration, particle number concentration, and particle mass concentrations) were measured by task in a laboratory making carbon nanotubes (CNTs) films using three direct reading instruments. Because of the conducted other researcher's experiment before the tasks, airborne nanoparticle surface area and number concentrations are the highest at the first time conducted weighing and mixing by sonication task, respectively. Because of the mist generated during mixing by sonication, the highest airborne nanoparticle surface area and PM1 concentrations were measured in the task among the total. Nanoparticle surface area concentrations at the researchers' breathing zones had high correlation (r=0.93, p<0.01) with those measured at an area in the laboratory. This result indicates that nanoparticles generated during the experiment contaminated the whole room air. When the experiment performed all the fume hoods weren't operated and making CNTs films task were conducted in the out of the fume hoods. In conclusion, researchers performing making CNTs film experiments were exposed to airborne nanoparticles generated during the experiment without adequate controls. We recommend that adequate controls should be implemented so that workers' exposures to airborne nanoparticle are limited to minimum levels.

• Journal of Distribution Science
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• v.16 no.11
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• pp.11-19
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• 2018

Purpose - The purpose of this study is to provide a systematic literature review related to environmental upgrading in Global Value Chains (GVCs) and suggest possible future research agendas in advancing environmental upgrading and ultimately GVC boundaries. Research design, data, and methodology - The academic databases such as Science Direct, EBSCO, ProQuest and Google Scholar were explored using a structured keywords searches to identify relevant research in the environmental upgrading area in GVCs. Only relevant papers were selected after reading the abstracts, and analyzed using qualitative content analysis. Results - Overall analysis of the literature review suggests two critical developments in the field of environmental upgrading. The first and foremost major development is an enhanced understanding of environmental upgrading as a concept and phenomenon. The second significant development is that environmental upgrading has been empirically proven to be fundamentally based on relationships and power structures within GVCs. Conclusions - Environmental upgrading in GVCs has been studied individually and not in relation to financial outcomes and social upgrading. Hence, the relationship of environmental upgrading with financial outcomes and social upgrading needs to be investigated. Furthermore, the impact of the interaction of varying institutional structures on environmental upgrading is worthy of future study.

• Journal of Families and Better Life
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• v.27 no.6
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• pp.21-29
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• 2009

This study investigated the effects of three factors-mothers' parenting beliefs; child care-home involvement; and the home learning environment - on the school readiness of 3- to 5-year-olds. The subjects were 366 children who were enrolled in child care centers located in Seoul and the Kyoungki area, and their mothers. The Structural Equation Modeling (SEM) technique was employed to test the pathways to children's school readiness as indicated by the child's abilities in vocabulary, math and reading. The results showed that mothers' stronger beliefs in their responsibilities in their children's academic and behavioral development predicted greater involvement in child care and better quality in the home learning environment. Likewise, the quality of the learning environment predicted the extent of the child's readiness for school. No direct relation was found between child care involvement and the child's school readiness. The results imply that multiple factors - parental, child-care-related, and home environmental- explain the extent to which the child is prepared to adjust to scholastic life.