• Title/Summary/Keyword: Dipping

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Characterization of Fusarium solani Causing Fusarium Root Rot of Lisianthus in Korea (꽃도라지 뿌리썩음병을 일으키는 Fusarium solani 의 특성)

  • Choi, Hyo-Won;Hong, Sung Kee;Lee, Young Kee;Kim, Jeomsoon;Lee, Jae Guem;Kim, Hyo Won;Kang, Eun Hye;Lee, Eun Hyeong
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.74-82
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    • 2017
  • Lisianthus (Eustoma grandiflorum) is a flowering ornamental plant used widely in Korea. In 2015, wilting, damping-off, stunting, and root rot symptoms were observed in lisianthus plants of Yeoju and Gimhae, Korea. Affected seedlings appeared yellow and showed poor development of root systems in the field and in nursery boxes. Furthermore, affected plants were yellow, stunted, and died at approximately 2-3 months after transplanting. Fusarium species were consistently isolated from the basal stems of diseased plants. Nine isolates were identified as Fusarium solani based on morphological characteristics. Macroconidia of isolates were relatively wide, straight-to-slightly curved, and microconidia formed in false heads on long monophialides. Abundant chlamydospores were produced at the middle or tips of hyphae. To confirm this identification, a molecular analysis of the translation elongation factor 1 alpha (TEF) and RNA polymerase II subunit (RPB2) genes was conducted. The sequences of TEF and RPB2 showed 99.2-99.9% and 98.0-98.1% similarity, respectively, to those of reference F. solani strains in NCBI GenBank. Pathogenicity was tested using root dipping inoculation of healthy lisianthus seedlings. Symptoms were observed within 7 days of inoculation only in inoculated plants. This is the first report of F. solani causing Fusarium root rot on lisianthus in Korea.

Occurrences of Ilmenite Deposits in Hadong-Sancheong Area (하동-산청 티탄철석 광상의 광체배태양상)

  • Koh, Sang-Mo
    • Journal of the Mineralogical Society of Korea
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    • v.23 no.1
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    • pp.25-37
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    • 2010
  • Ilmenite ore bodies are deposited within the Precambrian anorthosite body distributed in the Hadonggun and Sancheonggun district, Gyeongsangnamdo. This study tries to identify the occurrence of ilmenite ore body in titanium mine area distributed in Wheolheongri, Okjongmyon, Hadonggun and six mining concession areas (Danseong claim no. 64, 65, 74, 75, 84, 85) in Danseongmyon, Sancheonggun. Wheolheongri ilmenite ore body occurs as vein with about 10~50 m width and 100 m length and shows NNE strike and NW dipping. High grade ore with $TiO_2$ 20 wt% in this area is distributed in intercumulated anorthosite and is sheared and brecciated. Ilmenite occurring in this type is commonly associated with hornbelnde. Ilmenite ore bodies distributed in Danseonggun, Sancheongmyon are deposited in layered anorthosite. They occur as stratiform with variable width from several and several tens meters. Ilmenite which is disseminated in the matrix is sheared and elongated. This type shows generally low grade ($TiO_2$ 1.0~6.0 wt%). The ilmenite ore bodies occur as vein and stratiform, and the former shows higher grade than the latter.

Biological Control of Crown Gall

  • Kerr, Allen;Biggs, John;Ophel, Kathy
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1994.06a
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    • pp.11-26
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    • 1994
  • Crown gall of stonefruit and nut trees is one of the very few plant diseases subject to efficient biological control. The disease is caused by the soil-inhabiting bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes and the original control organism was a non-pathogenic isolate of A. rhizogenes strain K84. Control is achieved by dipping planting material in a cell suspension of strain K84 which specifically inhibits pathogenic strains containing a nopaline Ti plasmid. Because the agrocin 84-encoding plasmid (pAgK84) is conjugative, it can be transmitted from the control strain to pathogenic strains which, as a result, become immune to agrocin 84 and cannot be controlled. To prevent this happening, the transfer genes on pAgK84 were located and then largely eliminated by recombinant DNA technology. The resulting construct, strain K1026, is transfer deficient but controls crown gall just as effectively as does strain K84. Field data from Spain confirm that pAgK84 can transfer to pathogenic recipients from strain K84 but not from strain K1026. The latter has been registered in Australia as a pesticide and is the first genetically engineered organism in the world to be released fro commercial use. It is recommended as a replacement for strain K84 to prevent a breakdown in the effectiveness of biological control of crown gall. Several reports indicate that both strains K84 and K1026 sometimes control crown gall pathogens that are resistant to agrocin 84. A possible reason for this is that both strains produce a second antibiotic called 434 which inhibits growth of nearly all isolates of A. rhizogenes, both pathogens and non-pathogens. Crown gall of grapevine is caused by another species, Agrobacterium vitis. It is resistant to agrocin 84 and cannot be controlled by strains K84 or K1026. It is different from other crown gall pathogens in several characteristics, including the fact that, although a rhizosphere coloniser, its also lives systemically in the vascular tissue of grapevine. Pathogen free propagating material can be obtained from tissue culture or, less surely, by heat therapy of dormant cuttings. A number of laboratories are searching for a biocontrol strain that will prevent, or at least delay, reinfection. A non-pathogenic A. vitis strain F/25 from South Africa looks very promising in this regard.

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Studies on the Preservation of Apple -Effect of Inorganic Compounds Applied in the Field on the Shelf-life of Apples- (사과 저장(貯藏)에 관한 연구(硏究) -무기영양(無機營養)이 사과의 저장(貯藏)에 미치는 영향-)

  • Park, Nou-Poung;Kim, Yun-Jin;Kim, Sung-Kih;Rhee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.9 no.1
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    • pp.13-18
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    • 1977
  • In order to improve the storage ability of Spur Red Delicious apple, calcium ingredients were applied to the apple tree during the growing period, and then the effects of calcium on appearances and respiratory activity of harvest apples were investigated during the storage period. The results of this work were summarized as follows: 1. The freshness of apples treated by calcium compounds reached $73{\sim}78%$, but that of control group was 68% by March of next year. 2. The respiratory rate of the apples that had been applied the high concentration of ammonium sulfate in field during the growing period, was accelerated rather than control group, and that of calcium treatment groups was continuously inhibited during the storage period. 3. The direct foliar application of calcium compounds in the field was more effective than dipping apples in calcium chloride solution for better quality and improving storage ability after harvest. 4. The freshness and hardness relatively not much decreased in calcium treatment groups even after 5 month storage.

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Effects of Mild Heat Treatment on Microorganisms, Respiratory Characteristics and Firmness of Fuji Apple (중온 열수 처리가 사과의 표면 미생물, 호흡특성 및 경도에 미치는 영향)

  • Seo, Ja-Young;Kim, En-Jeong;Hong, Seok-In;Yu, Sung-Hun;Kim, Dong-Man
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.47-51
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    • 2006
  • Microorganisms involved in decaying Fuji apples during storage were investigated. Seven pathogens were isolated from the rotted fruits. Penicillium spp. was derived from 65-75% of decayed apples with P. expansum being dominant species. Effects of mild heat treatment on microbial reduction, respiration, and texture characteristics in Fuji apples were examined through hot water dipping at $40-65^{\circ}C$ for varied timε periods. Initial counts of total microorganisms and moulds in fresh fruits s showed 4.75 and 4.66 log CFU/g in a stem, as well as 5.35 and 4.32 log CFU/g in a calyx, respectively. The heat treatment at $40^{\circ}C$ for 180 min significantly reduced the population of total microorganisms and moulds in the fruits. Respiration rate of the apple fruits increased immediately after heat treatment and then returned to the normal level during storage. The rates of ethylene production in the fruits treated at $40-50^{\circ}C$ were maintained lower than that of the untreated control. The fruits treated at $40^{\circ}C$ showed slightly greater flesh firmness than the other apple samples during storage.

Effect of Life or Death Condition before Cooling on Physicochemical Properties of Plaice, Paralichthys olivaceus Muscle at the Early Period after Death (냉각전에 생사의 차이가 넙치육의 사후조기의 물리$\cdot$화학적변화에 미치는 영향)

  • CHO Yong-Je;LEE Ho-Su;YOU Seung-Geun;KIM Tae-Jin;LEE Nam-Gul;CHOI Young-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.4
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    • pp.523-528
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    • 1998
  • To clarify the effect of life or death condition before cooling on the physicochemical properties of plaice, Paralichthys olivaceus muscle at the early period after death, the plaices were dipped in the refrigerated sea water ($0^{\circ}C$) either as alive or after anesthesia killing. These samples were stored at $0^{\circ}C$ sea water and the changes in rigor-mortis, ATP breakdown, content of ATP and its related compounds, breaking strength and lactate accumulation through storage were investigated. Acceleration of rigor-mortis, ATP breakdown and lactate accumulation were taster in the samples refrigerated as alive than in samples killed by anesthesia before cooling. ATP in samples refrigerated as alive showed little breakdown until 7.5 hrs but it was decomposed completely after 17.5 hrs storage. The breaking strength in muscle of plaice was 1736.2 $\pm$ 65.4 g immediately after killing. The breaking strength in samples dipped in refrigerated sea water as alive increased more rapidly and showed the maximum value over 7.5 hrs (2183.3$\pm$32.2 g), However, in case of samples killed by anesthesia before cooling, the value and time reached around the maximum breaking strength were 2126.3 $\pm$ 32.2 g and 12.5 hrs, respectively and then decreased until 30 hrs. From these results, it could be suggested that dipping in refrigerated sea water after anesthesia killing before cooling is more effective in maintaining freshness of fresh plaice muscle than refrigerating as alive.

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A Study of System and Practices of the Old Age Pension in North Korea (북한 년로년금의 제도와 실태에 관한 연구)

  • Min, Ki-Chae;Cho, Sung-Eun;Han, Kyoung-Hun
    • Korean Journal of Family Social Work
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    • no.60
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    • pp.133-173
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    • 2018
  • This paper analyzes the consistency and the discrepancy between system and practices of the old age pension(Nyunronyungeum) in North Korea. The literature review is conducted to analyze the system. Specifically, the North Korean law and North Korean dictionaries were carefully examined. The interviews with 25 North Korean refugees were conducted to grasp the exact state of the old age pension in North Korea. Major findings are as follows: the consistency between system and practices of the old age pension in North Korea is identified only certain portions of that. Beneficiaries(blue-collar workers, white-collar workers, farmers, soldiers, and employees in foreign-invested enterprises), contribution periods, earning-related schemes, the totalization of periods of coverage(workplace transitions), the absence of double benefits and early retirement pension, and the delivery system around Civic Service offices(Dong offices) are confirmed by complete consistency. Preservation age, variations in the implementation of the pension system by region, and premium of the old age pension are confirmed by partial consistency. The length of service, the labor regulation, lump-sum payment, and double dipping are confirmed by discrepancies. It's important that this study brings about a better understanding the old age pension in North Korea through various original texts of North Korea and interviews with refugees. The results of this study are expected to contribute to the policy production for the age income security system after the unification and to the spread of unification perspectives.

Quality Changes in Fresh-Cut Potato (Solanum tuberosum var. Romano) after Low-Temperature Blanching and Treatment with Anti-Browning Agents (저온 blanching 및 갈변저해물질의 처리에 따른 최소가공 감자의 저장 중 품질변화)

  • Hwang, Tae-Young;Jang, Ji-Hyun;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.499-505
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    • 2009
  • Quality changes in fresh-cut potatoes during storage at $4^{\circ}C$ after treatment with low-temperature blanching and antibrowning agents were studied. Fresh-cut potatoes were treated by dipping for 1.5 min in a browning inhibitor solution containing 0.5% (w/v) ascorbic acid, 0.5% (w/v) citric acid, 0.5% (w/v) sodium chloride, 0.1% (w/v) trehalose, and 0.005% (w/v) biotin, at $60^{\circ}C$, with subsequent cooling for 1.5 min and storage at $4^{\circ}C$. The browning properties of fresh-cut potatoes were examined by measurement of polyphenol oxidase (PPO) activity and total phenolic content. Changes in quality attributes over a 14-day period were assessed in terms of titratable acidity, pH, water-soluble solid level, and gas analysis at $4^{\circ}C$. During storage, PPO activity increased, with the lowest activity seen after about 7 days of storage. Treatment with antibrowning solution at $4^{\circ}C$ increased visual sensory attributes during storage. Low-temperature blanching in distilled water more effectively inhibited browning compared with exposure to browning inhibitor solution, as assessed after 7 days of storage. Fresh-cut potatoes respired aerobically after different treatments during storage at $4^{\circ}C$.

Quality Characteristics of Seaweed Kimchi Containing Kjellmaniella crassifolia and Mytilus coruscus Seasoning (Kjellmaniella crassifolia와 Mytilus coruscus 조미액을 첨가한 해조김치의 품질특성)

  • Kim, Ki-Woong;Bae, Tae-Jin
    • Journal of Chitin and Chitosan
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    • v.22 no.4
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    • pp.254-263
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    • 2017
  • In order to improve the utilization of seaweeds, two kinds of mixed seasoning concentrates (KMS, MKS) were prepared with Kjellmaniella crassifolia and Mytilus coruscus as main ingredients. Fermentation experiments were carried out at $4^{\circ}C$ for 70 days with Kimchi. As a result, SK-A showed a decrease in pH and an increase in acidity. Salinity was not significantly different after 70th day. Reducing sugar content was higher in mixed seasoning group. Lactic acid bacteria Leuconostoc sp. and Lactobacillus sp. Increased in the control group after 20 days of fermentation. The lightness was higher in the control group from the early stage of dipping to 30 days, and the degree of yellowing was higher than that of SK-B group from 20 days. The hardness of the SK-A group was high at the early stage of immersion and at 10th day. The content of aspartic acid and glutamic acid in free amino acid SK-A group was significantly higher (p<0.05). The content of sweet amino acids was significantly higher in the SK-A group on the 0th, 10th, and 30th days of fermentation than the other groups (p<0.05). The sensory test results showed that SK-A showed the most favorable taste. As a result of reviewing the above results, it is expected that SK-A group immersed in 100 g of seasoning liquid KMS made from Kjellmaniella crassifolia and Mytilus coruscus as a subordinate material will provide taste and nutrition to consumers.

Ductile Shear Deformation around Jirisan Area, Korea (지리산 일대의 연성전단변형)

  • Ryoo, Chung-Ryul;Kang, Hee-Cheol;Lee, Sang-Won
    • The Journal of the Petrological Society of Korea
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    • v.28 no.2
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    • pp.53-69
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    • 2019
  • In the Jirisan area of the Yeongnam Massif, Korea, several ductile shear zones are developed within Precambrian gneiss complex (Jirisan metamorphic rock complex). The ductile shear zones have a general NS- and NNE-striking foliation with westward dipping directions. The foliation developed in the shear zones cut the foliation in gneiss complex. The stretching lineations are well developed in the foliated plane of the shear zone, showing ENE-trend with gentle plunging angle to the ESE direction. Within shear zone, several millimetric to centimetric size of porphyroclasts are deformed strongly as a sigmoid form by ductile shearing. The sigmoid patterns of porphyroclasts in the shear zones indicate the dextral shearing. The spatial distribution of ductile shear zone is characterized by the dominant NS- and NNE-striking dextral sense in the central and eastern regions respectively. In the western part, it develops in NE-striking dextral sense which is the general direction of the Honam shear zone. The U-Pb concordant ages obtained from the two samples, the strongly sheared leucocratic gneiss, are $1,868{\pm}3.8Ma$ and $1,867{\pm}4.0Ma$, respectively, which are consistent with the U-Pb ages reported around the study area. We supposed that the ductile shearing in the study area is occurred about 230~220 Ma during late stage of the continental collision around Korea and is preceded by granitic intrusion related to subduction during 260~230 Ma, which are supported by compiling the age data from sheared gneiss, deformed mafic dyke intruded gneiss complex, and non-deformed igneous rocks.