• Title/Summary/Keyword: Dimethyl sulfoxide

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Glycerol-Spore Formation of Myxobacteria in the Presence of DMSO and Glycerol (DMSO와 Glycerol 처리에 따른 점액세균의 글리세롤포자 형성능 조사)

  • Jang, Eun-Hae;Kim, Do-Hee;Kim, Jin-Woo;Cho, Kyung-Yun
    • Microbiology and Biotechnology Letters
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    • v.34 no.4
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    • pp.335-337
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    • 2006
  • Glycerol-spore formation of wild myxobacteria isolated in Korea has been tested. One third of the wild myxobacterial isolates belonged to the genus Myxococcus and Corallococcus produced a large number of glycerol-spores in the presence of glycerol or dimethyl sulfoxide. However, two third of the other wild myxobacterial isolates belonged to the genus Myxococcus and Corallococcus produced a negligible number of spores under the same condition. In addition, the myxobacteral isolates belonged to the other genus, such as Stigmatella, Archangium, and Cystobacter, failed to produce a noticeable number of glycerol-spores.

Synthesis of Schiff-Base Ligands and Determination of Stability Constants of Their Transition Metal(II) Complexes (질소-산소계 시프염기 리간드의 합성과 전이금속(II) 착물의 안정도상수결정)

  • Kim, Seon Deok;Song, Chan Ik;Kim, Jun Gwang;Kim, Jeong Seong
    • Journal of Environmental Science International
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    • v.13 no.9
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    • pp.835-843
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    • 2004
  • N,N-bis(2-salicylaldehyde)dipropylenetriamine(5- Hsaldipn), N,N-bis( 5-bromosalicyl-aldehyde) dipropylenetriamine (5-Brsaldipn), N,N-bis(5-chlorosalicy laldehyde )dipropylene-triamine(5-Clsaldipn), N,N-bis(2-hydroxy- $5-methoxy-benzaldehyde)dipropylenetriamine(5-OCH_3saldipn)$ and N,N-bis (2-hydroxy-5-nitrobenzaldehyde)dipropylenetriamine $(5-NO_2saldipn)$ were synthesized and characterized by elemental analysis, infrared spectrometry, NMR spectrometry and mass spectrometry. Their proton dissociation constants were determined in 70% dioxane/30% water solution by potentiometric. Stability constants of the complexes between these ligands and the metal ions such as Cu(II), Ni(II) and Zn(II) were measured in dimethyl sulfoxide by a polarographic method. Stability constants for the ligands were in the order of $5-OCH_3$ > 5-H > 5-Br > 5-Cl > $5-NO_2$ saldipn. Enthalpy and entropy changes were obtained in negative values.

Bacterial Virus DNA Damage Caused by Fumonisin B1 (Fumonisin B1에 의한 세균바이러스 DNA손상)

  • 이길수;조성국
    • Environmental Mutagens and Carcinogens
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    • v.19 no.1
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    • pp.34-38
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    • 1999
  • Fumonisin B1 is a secondary metabolite of Fusarium moniliforme, a contaminant of corn and corn product. Fumonisin B1 has been shown to be responsible for major toxicological effects of the fungus in rats, horses, and pigs. Fumonisin B1 induced λ DNA fragmentation, which was increased with incubation time, reducing agent NADPH and metal ion (Cu2+). The DNA damage was inhibited by dimethyl sulfoxide (DMSO) or mannitol as radical scavenger for free radicals. DNA fragmentation, induced by fumonisin B1 in the presence of 1 mM NADPH and 0.1 mM CuCl2, was inhibited by 100 mM DMSO. By the in vitro reaction of fumonisin B1 with supercoiled plasmid pBR322 DNA, plasmid DNA was relaxed, eventually linearized in the agarose gel electrphoresis. From rifampicin sensitive E. coli CSH138 in bacterial mutagenesis system, the rifampicin resistant E. coli mutants were obtained by fumonisin B1. These results suggest that fumonisin B1 may be a possible environmental mutagen in bacterial mutagen assay system.

Characterization of Agarose Product from Agar Using DMSO

  • Jeon, You-Jin;Athukorala, Yasantha;Lee, Je-Hee
    • ALGAE
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    • v.20 no.1
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    • pp.61-67
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    • 2005
  • Agar was extracted from Gelidium amansii, which was harvested at the shores of Jeju Island in South Korea. As a unique solvent, the ability of dimethyl sulfoxide (DMSO) was used to separate agarose from agar by removing agaropectine and quality of the resultant agarose was characterized for chromatography purposes. Agar sample was agitated by motor-driven stirrer with DMSO in a water bath (at 70$^{\circ}C$ for 2 h) and centrifuged (3,000 rpm for 20 min). Resultant upper agarose layer was gelled, washed, dried and milled. The quality of agarose was evaluated by the analysis of proximate chemical composition, sulfate content, gelling strength and DNA migration. In this study, the separated agarose showed low sulfate amount (0.28%) and showed high gel strength (1190 g ${\cdot}\;cm^{-2}$). The resolution power and the ligase activities gave clear picture about the suitability of the present agarose for practical purposes.

Preparation of Porous PLGA Microfibers Using Gelatin Porogen Based on a Glass Capillary Device (젤라틴 기공유도물질과 유리모세관 장치를 이용한 다공성 PLGA 미세섬유의 제조)

  • Kim, Chul Min;Kim, Gyu Man
    • Journal of the Korean Society for Precision Engineering
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    • v.33 no.1
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    • pp.63-67
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    • 2016
  • We present a method of fabricating poly (lactic-co-glycolic acid) (PLGA) porous microfibers using a pore template. PLGA microfibers were synthesized using a glass capillary tube in a poly-(dimethylsiloxane) (PDMS) microfluidic chip. Gelatin solution was used as a porous template to prepare pores in microfibers. Two phases of PLGA solutions in different solvents-DMSO (dimethyl sulfoxide) and DCM (dichloromethane)-were used to control the porosity and strength of the porous microfibers. The porosity of the PLGA microfibers differed depending on the ratio of flow rates in the two phases. The porous structure was formed in a spiral shape on the microfiber. The porous structure of the microfiber is expected to improve transfer of oxygen and nutrients, which is important for cell viability in tissue engineering.

Physico-chemical Characteristics and Antiviral Activity of ASA, an Antibiotic Produced by Actinomycetes B25 (방선균 B25 균주가 생산하는 항생물질 ASA의 물리.화학적 특성 및 항바이러스 활성)

  • 여운형;김영호;박은경;김상석
    • Korean Journal Plant Pathology
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    • v.13 no.1
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    • pp.63-68
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    • 1997
  • In the screening of antiviral materials produced by actinomycetes, an isolate named B25 was fond to produce an antibiotic substance ASA, which showed a strong inhibitory activity against tobacco mosaic virus (TMV) infection. ASA was purified from culture broth of B25 by silica gel column chromatography, preparative TLC, and reversed phase HPLC. Also MS, IR, UV spectrum, and melting point of ASA were determined and analysed. ASA was white powder soluble in dimethyl sulfoxide, chloroform, and ethyl acetate, having absorption peaks at 223 and 328 nm in UV-VIS spectrum, and had a molecular weight of 548. ASA showed strong inhibitory effect on TMV infection when it was applied as a mixture of TMV to the upper surface of leaves of a local lesion host (Nicotiana tabacum c. Xanthi-nc). It also showed antimicrobial effect against yeast and some phytopathogenic fungi.

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Graft Copolymerization of Methacrylonitrile(MAN) onto Kevlar 49 Fiber Surface (Kevlar 49 섬유 표면에 대한 MAN의 Graft 공중합에 관한 연구)

  • 김은영;강주영;최재혁;김한도
    • Textile Coloration and Finishing
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    • v.7 no.1
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    • pp.43-50
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    • 1995
  • The grafting of methacrylonitrile(MAN) onto Kevlar 49 filament surface was carried out by anionic polymerization using sodium methylsulfinylcarbanion formed from sodium hydride and dimethyl sulfoxide(DMSO). The effects of reaction conditions on the grafting percentage(GP) and on the tensile strength of the fiber were investgated. GP marktedly increased with increasing metalation time, and NaH concentration, polymerization temperature and time. The tensile strength of fiber decrased with increasing metalation time, and NaH concentration, polymerization temperature and time. The optimum conditions to increase over 40% of GP with below 10% reduction rate of tensile strength of fiber : NaH concentration ; 30.6 mmol/l/0.5g Kevlar, metalation time : 10min, polymerization tempera- ture : 5$0^{\circ}C$, polymerization time: 20 sec, monomer concentration : 1.12mol/l/0.5g Kevlar.

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Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival

  • Choi, Youn Hee;Nam, Taek Jeong
    • Fisheries and Aquatic Sciences
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    • v.18 no.4
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    • pp.417-420
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    • 2015
  • We investigated the relationship between viability and IGF-1 receptor (IGF-1R) activity in D-shaped and umbo larvae of the surf clam Spisula sachalinensis after treatment with vitrification solution (VS) or freezing. In a toxicity assay, VS1, containing 5 M dimethyl sulfoxide (DMSO), was very harmful to D-shaped and umbo larvae. However, VS2, containing 5 M ethylene glycol (EG), was not harmful to either larval stage. Although VS2 had a promising toxicity test outcome, none of the larvae survived vitrification. After immersion into VSs and freezing, IGF-1R ${\beta}$-subunits were detected in all larvae; however, tyrosine phosphorylation of intracellular ${\beta}$-subunits was detected only in the control and live groups. These results suggest that activation of IGF-1R may influence surf clam larvae viability.

The release of cellular constituents of Lactobacillus lactis by freezing and freeze-drying

  • Jeong, Hyun-Do;Pack, Moo-Young
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1979.10a
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    • pp.246.2-246
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    • 1979
  • Lactobacillus lactis cell was markedly damaged when shocked by freezing and freeze-drying. The supernatant of shocked cells in 1% phosphate buffer (pH7.3) showed a maximum u.v. absorption spectra at 260nm after further incubation for 90min at $37-^{\circ}C.$ The leakage of cellular constituents by shocking could be prevented by dimethyl sulfoxide, glycerol, dextrose and $MgCl_2.$ Among them, $MgCl_2$ showed the preventing effect before and after freezing wh-ereas the rests, only before the treatment. This leakage was proved not to be related to the cellular repairing mechanism.

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Steady State Design for the Separation of Acetone-Chloroform Maximum Boiling Azeotrope Using Three Different Solvents

  • Pokhrel, Manish;Owusu, Asante Daniel;Cho, Jungho
    • Korean Chemical Engineering Research
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    • v.55 no.4
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    • pp.490-496
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    • 2017
  • We have designed an extractive distillation for separating maximum boiling azeotrope of acetone-chloroform system. PRO/II 9.4 was used to simulate the overall process. The VLE data adopted from Dortmund data bank was regressed to obtain a new set of binary interaction parameters. Three different entrainers were used for the separation process--dimethyl sulfoxide (DMSO), ethylene glycol (EG) and benzene--to test their viability for the acetone-chloroform system. Thermodynamic feasibility analysis was done through ternary map diagrams. Two different thermodynamic models, NRTL and UNIQUAC, were explored for the study of overall process.