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http://dx.doi.org/10.4490/ALGAE.2005.20.1.061

Characterization of Agarose Product from Agar Using DMSO  

Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
Athukorala, Yasantha (Faculty of Applied Marine Science, Cheju National University)
Lee, Je-Hee (Faculty of Applied Marine Science, Cheju National University)
Publication Information
ALGAE / v.20, no.1, 2005 , pp. 61-67 More about this Journal
Abstract
Agar was extracted from Gelidium amansii, which was harvested at the shores of Jeju Island in South Korea. As a unique solvent, the ability of dimethyl sulfoxide (DMSO) was used to separate agarose from agar by removing agaropectine and quality of the resultant agarose was characterized for chromatography purposes. Agar sample was agitated by motor-driven stirrer with DMSO in a water bath (at 70$^{\circ}C$ for 2 h) and centrifuged (3,000 rpm for 20 min). Resultant upper agarose layer was gelled, washed, dried and milled. The quality of agarose was evaluated by the analysis of proximate chemical composition, sulfate content, gelling strength and DNA migration. In this study, the separated agarose showed low sulfate amount (0.28%) and showed high gel strength (1190 g ${\cdot}\;cm^{-2}$). The resolution power and the ligase activities gave clear picture about the suitability of the present agarose for practical purposes.
Keywords
agar; agaropectine; agarose; DMSO; DNA migration; gel strength;
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1 Vonshak A. 1997. Microalgal Biotechnology; New Development in Production Facilities and Product. Abstracts of 2nd APMBC and 3rd APCAB. Phuket, Thailand. 21 pp
2 Walz H.G. 1999. Phytoplankton Analyzer Phyto-PAM: System Components and Principles of Operation. Walz, Effeltrich
3 김미경, 2005. 생물활성수를 함유한 배지조성물 및 그 제조방법. 특 허등록번호 제05003332호
4 김미경, 김영환. 1999. 일반조류학. 도서출판 아카데미서적 229 pp
5 김세권, 백호철, 변희국, 강옥주, 김종해. 2001. 해양미세조류의 생 화학적 조성 및 항산화성. 한국수산학회지 34: 260-267
6 박미경, 이석준, 서현효, 김희식, 김영호, 윤병대, 오희목. 1998. Scenedesmus quadricauda를 이용한 축산폐수의 고도처리. 한국 조류학회지 13: 227-233
7 오희목, 이석준. 1998. 미세조류를 이용한 생물공학산업의 현황과 전망. 한국해양미세조류은행의 현황과 전망, 한국해양미세조 류은행 부경대학교 수산과학연구소 Proceedings 49-65
8 이인규, 부성민, 이상희. 2004. 조류의 다양성과 계통. 라이프 사이 언스 262 pp
9 임진영, 조만기, 한봉호. 1998. 해수산 Chlorella의 최적 배양 조건에 관한 연구. 한수지 31: 139-142
10 환경부. 2001. 공정시험방법. 669 pp
11 APPA, AWWA. WEF. 1995. Standard methods for the examination of water and wastewater. In: Eaton A.D., Clesceri L.S. and Greenberg A.E. (eds), Baltimore 10: 157 pp
12 Gilberst E. and Hoffmann-Glewe S. 1991. Ozonation of ethylenediaminetetraacetic acid (EDTA) in aqueous s olution influence of pH value and metal ions. Water Research 24: 39-44   DOI   ScienceOn
13 Guillard R.R.L. 1975. Division rates. In: Stein J.R. (ed), Handbook of Phycological Methods-Culture Methods and Growth Measurements. Cambridge University Press, Cambridge. pp 289-311
14 Kim M.K., Park J.W., Park C.S., Jeune S.M., Kim S.J., Jeune K.H., Jang M.U. and Acreman J. 2006. Enhanced production of Scenedesmus spp. (green microalgae) using a new medium containing fermented swine wastewater. Bioresources Technol. (accepted)
15 John D.M., Whitton B.A. and Brook A.J. 2002. The Freshwater of Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae. Cambridge Unicersity Press, Cambridge
16 Kawamoto S. 1996. Experiment Results of BMW Techniques in the Farm of Kamegawa. Ecopeace Press, Daegu
17 Kim M.K. and Giraud G. 1989. Characters of neutral lipids of Detonula sp. (Diatom) in culture. The Korean J. of Phycol. 4: 55-61
18 Kim M.K. and Smith R.E.H. 2001. Effect of ionic copper toxicity on the growth of green alga, Selenastrum capricornutum. J. Microbiol. Biotechnol. 11: 211-216
19 Kim M.K. and Thomas J.C. 1991. Studies of growth according to the concentration of mineral elements of medium in Cyanophyte SG 63. Korean J. Bot. 35: 1-8
20 Lee K. and C.G. Lee. 2001. Effect of light/dark cycles on wastewater treatment by microalgae. Biotechnol. Bioprocess Eng. 6: 194-199   DOI   ScienceOn
21 Nagasaki H. 1998. Bacteria Save the Earth Challenge of BMW Technology, Ecopeace Press, Daegu
22 Parsons T.R. and Strickland J.D.H. 1963. Discussion of spectrophotometric determination of marine-plant pigment, with revised equation for ascertaining chlorophylls and carotenoids. J. Mar. Res. 21: 155-163
23 Shon Y.H., Nam K.S. and Kim M.K. 2004. Cancer chemopreventive potential of Scenedesmus cultured in medium based on swine wastewater. J. Microbiol. Biotechnol. 14: 158-161
24 Sung K.D., Lee J.S., Shin C.S. and Park S.C. 1998. Enhanced cell growth of Chlorella sp. KR-1 by the addition of iron and EDTA. J. Microbiol. Biotechnol. 8: 409-411
25 Jeffrey S.W. 1972. Preparation and son properties of crystalline chlorophyll $c_{1}$ and $c_{2}$ from marine algae. Biochim. Biophys. Acta., 279: 15-33   DOI   ScienceOn
26 안주희, 김성수, 김태호, 이준엽, 오상집, 이진하, 이현용. 1996. 축 산폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화. 산업미생물학회지 24: 519-524
27 Kim M.K., Dubacq J.P., Thomas J.C. and Giraud G. 1996. Seasonal variation of triacylglycerols and fatty acids in Fucus serratus. Phytochemistry 43: 49-55   DOI   ScienceOn
28 Holm-Hansen O., Lorenzen C.J., Holmes R.W., and Strickland J.D.H. 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer. 30: 3-15   DOI