Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
권호 표지
DOI QR코드

DOI QR Code

Preparation of Porous PLGA Microfibers Using Gelatin Porogen Based on a Glass Capillary Device

젤라틴 기공유도물질과 유리모세관 장치를 이용한 다공성 PLGA 미세섬유의 제조

  • Kim, Chul Min (School of Mechanical Engineering, Kyungpook National University) ;
  • Kim, Gyu Man (School of Mechanical Engineering, Kyungpook National University)
  • 김철민 (경북대학교 기계공학부) ;
  • 김규만 (경북대학교 기계공학부)
  • Received : 2015.10.30
  • Accepted : 2015.11.14
  • Published : 2016.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

We present a method of fabricating poly (lactic-co-glycolic acid) (PLGA) porous microfibers using a pore template. PLGA microfibers were synthesized using a glass capillary tube in a poly-(dimethylsiloxane) (PDMS) microfluidic chip. Gelatin solution was used as a porous template to prepare pores in microfibers. Two phases of PLGA solutions in different solvents-DMSO (dimethyl sulfoxide) and DCM (dichloromethane)-were used to control the porosity and strength of the porous microfibers. The porosity of the PLGA microfibers differed depending on the ratio of flow rates in the two phases. The porous structure was formed in a spiral shape on the microfiber. The porous structure of the microfiber is expected to improve transfer of oxygen and nutrients, which is important for cell viability in tissue engineering.

Keywords

References

  1. Khademhosseini, A., Langer, R., Borenstein, J., and Vacanti, J. P., "Microscale Technologies for Tissue Engineering and Biology," Proc. of the National Academy of Sciences of the United States of America,Vol. 103, No. 8, pp. 2480-2487, 2006. https://doi.org/10.1073/pnas.0507681102
  2. Mooney, D. J. and Vandenburgh, H., "Cell Delivery Mechanisms for Tissue Repair," Cell Stem Cell, Vol. 2, No. 3, pp. 205-213, 2008. https://doi.org/10.1016/j.stem.2008.02.005
  3. Patrick Jr, C. W., Chauvin, P. B., Hobley, J., and Reece, G. P., "Preadipocyte Seeded PLGA Scaffolds for Adipose Tissue Engineering," Tissue Engineering, Vol. 5, No. 2, pp. 139-151, 1999. https://doi.org/10.1089/ten.1999.5.139
  4. Holy, C. E., Cheng, C., Davies, J. E., and Shoichet, M. S., "Optimizing the Sterilization of PLGA Scaffolds for Use in Tissue Engineering," Biomaterials, Vol. 22, No. 1, pp. 25-31, 2000. https://doi.org/10.1016/S0142-9612(00)00136-8
  5. Astete, C. E. and Sabliov, C. M., "Synthesis and Characterization of PLGA Nanoparticles," Journal of Biomaterials Science, Vol. 17, No. 3, pp. 247-289, 2006. https://doi.org/10.1163/156856206775997322
  6. Kim, H. G., Kim, K. M., Kim, Y. H., Lee, S. H., and Kim, G. M., "Preparation of Monodisperse ENXLoaded PLGA Microspheres Using a Microfluidic Flow-Focusing Device," Journal of Biobased Materials and Bioenergy, Vol. 7, No. 1, pp. 108-114, 2013. https://doi.org/10.1166/jbmb.2013.1263
  7. Ryu, T.-K., Oh, M.-J., Moon, S.-K., Paik, D.-H., Kim, S.-E., et al., "Uniform Tricalcium Phosphate Beads with an Open Porous Structure for Tissue Engineering," Colloid Surface B: Biointerfaces, Vol. 112, No. 12, pp. 368-373, 2013. https://doi.org/10.1016/j.colsurfb.2013.08.023
  8. Hwang, C. M., Khademhosseini, A., Park, Y., Sun, K., and Lee, S.-H., "Microfluidic Chip-Based Fabrication of PLGA Microfiber Scaffolds for Tissue Engineering," Langmuir, Vol. 24, No. 13, pp. 6845-6851, 2008. https://doi.org/10.1021/la800253b
  9. Astaneh, R., Erfan, M., Moghimi, H., and Mobedi, H., "Changes in Morphology of in Situ Forming PLGA Implant Prepared by Different Polymer Molecular Weight and Its Effect on Release Behavior," Journal of Pharmaceutical Sciences, Vol. 98, No. 1, pp. 135-145, 2009. https://doi.org/10.1002/jps.21415
  10. Qutachi, O., Vetsch, J. R., Gill, D., Cox, H., Scurr, D. J., et al., "Injectable and Porous PLGA Microspheres That Form Highly Porous Scaffolds at Body Temperature," Acta Biomaterialia, Vol. 10, No. 12, pp. 5090-5098, 2014. https://doi.org/10.1016/j.actbio.2014.08.015
  11. Kang, S.-W., La, W.-G., and Kim, B.-S., "Open Macroporous Poly (lactic-co-glycolic acid) Microspheres as an Injectable Scaffold for Cartilage Tissue Engineering," Journal of Biomaterials Science, Polymer Ed., Vol. 20, No. 3, pp. 399-409, 2009. https://doi.org/10.1163/156856209X412236
  12. Onoe, H., Okitsu, T., Itou, A., Kato-Negishi, M., Gojo, R., et al., "Metre-Long Cell-Laden Microfibres Exhibit Tissue Morphologies and Functions," Nature Materials, Vol. 12, No. 6, pp. 584-590, 2013. https://doi.org/10.1038/nmat3606
  13. Choi, S.-W., Zhang, Y., Yeh, Y.-C., Wooten, A. L., and Xia, Y., "Biodegradable Porous Beads and Their Potential Applications in Regenerative Medicine," Journal of Materials Chemistry, Vol. 22, No. 23, pp. 11442-11451, 2012. https://doi.org/10.1039/c2jm16019f
  14. Huang, C.-C., Wei, H.-J., Yeh, Y.-C., Wang, J.-J., Lin, W.-W., et al., "Injectable PLGA Porous Beads Cellularized by Hafscs for Cellular Cardiomyoplasty," Biomaterials, Vol. 33, No. 16, pp. 4069-4077, 2012. https://doi.org/10.1016/j.biomaterials.2012.02.024
  15. Kim, C. M. Ullah, A., and Kim, G. M. "Preparation of Highly Porous PLGA Microparticles Using Droplet Fission and Gelatin Porogen," Proc. of 41st Micro and Nano Engineering, 2015.
  16. Kim, T. K., Yoon, J. J., Lee, D. S., and Park, T. G., "Gas Foamed Open Porous Biodegradable Polymeric Microspheres," Biomaterials, Vol. 27, No. 2, pp. 152-159, 2006. https://doi.org/10.1016/j.biomaterials.2005.05.081
  17. Kim, C. M., Park, S. J., and Kim, G. M., "Applications of PLGA Microcarriers Prepared Using Geometrically Passive Breakup on Microfluidic Chip," Int. J. Precis. Eng. Manuf., Vol. 16, No. 11, 2015.
  18. Zhang, Q., Tan, K., Ye, Z., Zhang, Y., Tan, W., et al., "Preparation of Open Porous Polycaprolactone Microspheres and Their Applications as Effective Cell Carriers in Hydrogel System," Materials Science and Engineering: C, Vol. 32, No. 8, pp. 2589-2595, 2012. https://doi.org/10.1016/j.msec.2012.07.045
  19. Teh, S.-Y., Lin, R., Hung, L.-H., and Lee, A. P., "Droplet Microfluidics," Lab on a Chip, Vol. 8, No. 2, pp. 198-220, 2008. https://doi.org/10.1039/b715524g
  20. Dang, T.-D., Kim, Y. H., Kim, H. G., and Kim, G. M., "Preparation of Monodisperse Peg Hydrogel Microparticles Using a Microfluidic Flow-Focusing Device," Journal of Industrial and Engineering Chemistry, Vol. 18, No. 4, pp. 1308-1313, 2012. https://doi.org/10.1016/j.jiec.2012.01.028
  21. Choi, J. H. and Kim, G. M., "Micro-Patterning on Non-Planar Surface Using Flexible Microstencil," Int. J. Precis. Eng. Manuf., Vol. 12, No. 1, pp. 165-168, 2011. https://doi.org/10.1007/s12541-011-0023-x