• Title/Summary/Keyword: Dimensional Control

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Effect of the pore size in a 3D bioprinted gelatin scaffold on fibroblast proliferation

  • Choi, Dong Jin;Park, Sang Jun;Gu, Bon Kang;Kim, Young-Jin;Chung, Seok;Kim, Chun-Ho
    • Journal of Industrial and Engineering Chemistry
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    • v.67
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    • pp.388-395
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    • 2018
  • Significant efforts have been applied toward fabricating three-dimensional (3D) scaffolds using 3D-bioprinting tissue engineering techniques. Gelatin has been used in 3D-bioprinting to produce designed 3D scaffolds; however, gelatin has a poor printability and is not useful for fabricating desired 3D scaffolds using 3D-bioprinting. In this study, we fabricated pore size controlled 3D gelatin scaffolds with two step 3D-bioprinting approach: a low-temperature ($-10^{\circ}C$) freezing step and a crosslinking process. The scaffold was crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The pore sizes of the produced 3D gelatin scaffolds were approximately 30% smaller than the sizes of the designed pore sizes. The surface morphologies and pore sizes of the 3D gelatin scaffolds were confirmed and measured using scanning electron microscopy (SEM). Human dermal fibroblasts (HDFs) were cultured on a 3D gelatin scaffold to evaluate the effect of the 3D gelatin scaffold pore size on the cell proliferation. After 14 days of culture, HDFs proliferation throughout the 3D gelatin scaffolds prepared with more than $580{\mu}m$ pore size was approximately 14% higher than proliferation throughout the 3D gelatin scaffold prepared with a $435{\mu}m$ pore size. These results suggested that control over the 3D gelatin scaffold pore size is important for tissue engineering scaffolds.

Study on the cavitation and noise characteristics by air injection in three dimensional hydrofoil (3차원 수중익에서 공기분사에 의한 캐비테이션 및 소음특성 연구)

  • Seol, Hanshin;Jeong, Hongseok
    • The Journal of the Acoustical Society of Korea
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    • v.40 no.1
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    • pp.38-45
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    • 2021
  • In this study, changes in cavitation pattern and noise by air injection were investigated experimentally in a cavitation tunnel. Air injection system that can control the location and the amount of air was manufactured and installed in an elliptic wing that exhibits similar characteristics to those of a propeller blade. Various types of cavitation were simulated on the hydrofoil by adjusting the test conditions in the cavitation tunnel, and the changes in cavitation pattern and noise according to air injection were experimentally analyzed. It was shown that the noise characteristics varied depending on the position and the amount of air injection. This means that in order to apply the air injection technology to the propeller, it is necessary to optimize the air injection location and the amount of injection according to the cavitation characteristics.

Comparison of irradiated and non-irradiated acellular dermal matrices in breast reconstruction under radiotherapy

  • Woo, Soo Jin;Ha, Jeong Hyun;Jin, Ung Sik
    • Archives of Plastic Surgery
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    • v.48 no.1
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    • pp.33-43
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    • 2021
  • Background Acellular dermal matrices (ADMs) have become an essential material for implant-based breast reconstruction. No previous studies have evaluated the effects of sterility of ADM under conditions of radiation. This study compared sterile (irradiated) and aseptic (non-irradiated) ADMs to determine which would better endure radiotherapy. Methods Eighteen male Balb/C mice were assigned to the control group with no irradiation (group 1) or one of two other groups with a radiation intensity of 10 Gy (group 2) or 20 Gy (group 3). Both sterile and aseptic ADMs were inserted into the back of each mouse. The residual volume of the ADM (measured using three-dimensional photography), cell incorporation, α-smooth muscle actin expression, and connective tissue growth factor expression were evaluated. The thickness and CD3 expression of the skin were measured 4 and 8 weeks after radiation. Results In groups 2 and 3, irradiated ADMs had a significantly larger residual volume than the non-irradiated ADMs after 8 weeks (P<0.05). No significant differences were found in cell incorporation and the amount of fibrosis between irradiated and non-irradiated ADMs. The skin was significantly thicker in the non-irradiated ADMs than in the irradiated ADMs in group 3 (P<0.05). CD3 staining showed significantly fewer inflammatory cells in the skin of irradiated ADMs than in non-irradiated ADMs in all three groups after 4 and 8 weeks (P<0.05). Conclusions Under radiation exposure, irradiated ADMs were more durable, with less volume decrease and less deposition of collagen fibers and inflammatory reactions in the skin than in non-irradiated ADMs.

Simultaneous Removal of Cadmium and Copper from a Binary Solution by Cathodic Deposition Using a Spiral-Wound Woven Wire Meshes Packed Bed Rotating Cylinder Electrode

  • Al-Saady, Fouad A.A.;Abbar, Ali H.
    • Journal of Electrochemical Science and Technology
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    • v.12 no.1
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    • pp.58-66
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    • 2021
  • Spiral-wound woven wire meshes packed bed rotating cylinder electrode was used for the simultaneous removal of cadmium (Cd) and copper (Cu) from a binary solution. The effects of weight percent of each metal on the removal and current efficiencies were studied at an operating current of 345A, while the effect of current on the removal efficiency of both metals was investigated at three levels of current (240, 345.and 400 mA). The experiments were carried out at constant rotation speed 800 rpm, pH = 3, and a total concentration of metals (500 ppm). The results showed that the removal efficiency of copper increased from 89% to 99.4% as its weight percent increased from 20% to100%. In a similar fashion, the removal efficiency of cadmium increased from 81% to 97% as its weight percent increased from 20% to100%. The results confirmed that the removal efficiency of any metals declined in the presence of the other. Increasing of current resulted in increasing the removal efficiency of both metals at different weight percents. The results confirmed that current efficiencies for removing of copper and cadmium simultaneously decline with increasing of electrolysis time and weight percent of cadmium or with decreasing the weight percent of copper. Current efficiency was higher at the initial stage of electrolysis for all weight percents of metals. The results showed that the decay of copper concentration was exponential at all weight percents of copper, confirming that the electrodeposition of copper is under mass transfer control in the presence of cadmium. While the decay of cadmium concentration was linear at lower weight percent of cadmium then changed to an exponential behavior at high weight percent of cadmium in the presence of copper.

Directions of mandibular canal displacement in ameloblastoma: A computed tomography mirrored-method analysis

  • Evangelista, Karine;Cardoso, Lincoln;Toledo, Italo;Gasperini, Giovanni;Valladares-Neto, Jose;Cevidanes, Lucia Helena Soares;de Oliveira Ruellas, Antonio Carlos;Silva, Maria Alves Garcia
    • Imaging Science in Dentistry
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    • v.51 no.1
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    • pp.17-25
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    • 2021
  • Purpose: This study was performed to investigate mandibular canal displacement in patients with ameloblastoma using a 3-dimensional mirrored-model analysis. Materials and Methods: The sample consisted of computed tomographic scans of patients with ameloblastoma (n=10) and healthy controls (n=20). The amount of mandibular canal asymmetry was recorded as a continuous variable, while the buccolingual (yaw) and supero-inferior (pitch) directions of displacement were classified as categorical variables. The t-test for independent samples and the Fisher exact test were used to compare groups in terms of differences between sides and the presence of asymmetric inclinations, respectively (P<0.05). Results: The length of the mandibular canal was similar on both sides in both groups. The ameloblastoma group presented more lateral (2.40±4.16 mm) and inferior (-1.97±1.92 mm) positions of the mental foramen, and a more buccal (1.09±2.75 mm) position of the middle canal point on the lesion side. Displacement of the mandibular canal tended to be found in the anterior region in patients with ameloblastoma, occurring toward the buccal and inferior directions in 60% and 70% of ameloblastoma patients, respectively. Conclusion: Mandibular canal displacement due to ameloblastoma could be detected by this superimposed mirrored method, and displacement was more prevalent toward the inferior and buccal directions. This displacement affected the mental foramen position, but did not lead to a change in the length of the mandibular canal. The control group presented no mandibular canal displacement.

A Comparative Study on Preference of the Korean Army's Flight Jacket According to Working Environment (근무 환경에 따른 육군 비행재킷의 선호도 비교 연구)

  • Choi, Hee Eun;Choi, Kueng-mi
    • Fashion & Textile Research Journal
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    • v.22 no.6
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    • pp.844-852
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    • 2020
  • This study is to understand the preferences of pilots, flight engineers and crew who work in the same aircraft but are exposed to different working environments and perform different mission operations in order to develop an ergonomic flight jacket. Based on a preliminary investigation, a survey of 107 pilots and 36 flight engineers and crew was conducted. The results are as follows; Pilots can control the temperature inside the cockpit, so they are less exposed to the cold when working, while flight engineers and crew are exposed to the cold more because they have many external tasks. The reason for the problem of the current flight jacket was a difference in ranking between two groups, but the highest ranking was poor dimensional suitability due to the habit of wearing layers of clothing. As a result of preferred design, there were significant differences between groups in the item of overall style. Pilots preferred a bomber jacket style(P:68.2%, E&C:44.4%), on the other hand, flight engineers and crew preferred a field jacket style(P:26.2%, E&C:55.6%)(p<.01). They preferred a stand collar(P:71.0%, E&C:86.1%), a fastener slider for a front fastening(P:62.6%, E&C:61.1%), fastener tape cuffs(P:54.2%, E&C:47.2%), a jacket with a softshell(P:86.9%, E&C:83.3%), fleece as softshell material(P:88.8%, E&C:69.4%), and fastener sliders as a attaching method(P:69.2%, E&C:61.1%). A hem fastening will be selected differently according to the overall style of outshell. Additionally, they preferred more than 5ea pockets(P:51.4%, E&C:44.4%), fastener sliders as pocket's fastenings(P:48.6%, E&C:61.1%), armpit ventilations(P:62.9%, E&C:58.5%). The results of above will be considered to design an ergonomic flight jacket.

Parametric Crack and Flexural Strength Analyses of Concrete Slab For Railway Structures Using GFRP Rebar (GFRP 보강근을 적용한 교량용 콘크리트 도상슬래브의 균열 및 휨강도 변수 해석)

  • Choe, Hyeong-Bae;Lee, Sang-Youl
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.34 no.6
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    • pp.363-370
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    • 2021
  • In this paper, we presented an optimized crack and flexural strength analysis of a glass-fiber reinforced polymer (GFRP) rebar, used as reinforcements for in-site railway concrete slabs. The insulation performance of a GFRP rebar has the advantage of avoiding the loss of signal current in an audio frequency (AF) track circuit. A full-scale experiment, and three-dimensional finite element simulation results were compared to validate our approaches. Parametric numerical results revealed that the diameters and arrangements of the GFRP rebar had a significant effect on the flexural strength and crack control performances of the concrete track slabs. The results of this study could serve as a benchmark for future guidelines in designing more efficient, and economical concrete slabs using the GFRP rebar.

Crossover from weak anti-localization to weak localization in inkjet-printed Ti3C2Tx MXene thin-film

  • Jin, Mi-Jin;Um, Doo-Seung;Ogbeide, Osarenkhoe;Kim, Chang-Il;Yoo, Jung-Woo;Robinson, J. W. A.
    • Advances in nano research
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    • v.13 no.3
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    • pp.259-267
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    • 2022
  • Two-dimensional (2D) transition metal carbides/nitrides or "MXenes" belong to a diverse-class of layered compounds, which offer composition- and electric-field-tunable electrical and physical properties. Although the majority of the MXenes, including Ti3C2Tx, are metallic, they typically show semiconductor-like behaviour in their percolated thin-film structure; this is also the most common structure used for fundamental studies and prototype device development of MXene. Magnetoconductance studies of thin-film MXenes are central to understanding their electronic transport properties and charge carrier dynamics, and also to evaluate their potential for spin-tronics and magnetoelectronics. Since MXenes are produced through solution processing, it is desirable to develop deposition strategies such as inkjet-printing to enable scale-up production with intricate structures/networks. Here, we systematically investigate the extrinsic negative magnetoconductance of inkjetprinted Ti3C2Tx MXene thin-films and report a crossover from weak anti-localization (WAL) to weak localization (WL) near 2.5K. The crossover from WAL to WL is consistent with strong, extrinsic, spin-orbit coupling, a key property for active control of spin currents in spin-orbitronic devices. From WAL/WL magnetoconductance analysis, we estimate that the printed MXene thin-film has a spin orbit coupling field of up to 0.84 T at 1.9 K. Our results and analyses offer a deeper understanding into microscopic charge carrier transport in Ti3C2Tx, revealing promising properties for printed, flexible, electronic and spinorbitronic device applications.

The effect of the digital manufacturing technique of cantilevered implant-supported frameworks on abutment screw preload

  • Altuwaijri, Shahad Mohammmed;Alotaibi, Hanan Nejer;Alnassar, Talal Mughaileth
    • The Journal of Advanced Prosthodontics
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    • v.14 no.1
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    • pp.22-31
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    • 2022
  • PURPOSE. The purpose of this study was to investigate the misfit and screw preload at the implant abutment connection of implant supported fixed dental prosthesis with cantilever (ICFDP) manufactured using different digital manufacturing techniques and to compare the screw preload before and after cyclic loading. MATERIALS AND METHODS. Mandibular jaw model with four intra-foraminal implants was scanned using digital scanner. Stereolithography file was used to design a framework with nonengaging (NE) abutments and 10 mm cantilever distal to one terminal implant. Five frameworks were constructed using combined digital-conventional techniques (CAD-cast), and five frameworks were constructed using three-dimensional printing (3DP). Additional CAD-cast framework was constructed in a way that ensures passive fit (PF) to use as control. Scanning electron microscope (SEM) measured the implant abutment connection misfit. Sixty screws were used on the corresponding frameworks. Screws were torqued and pre-cyclic loading reverse torque value (RTV) was recorded. Frameworks were subjected to 200,000 loading cycles with a loading point 9 mm from the center of terminal implants adjacent to the cantilever and post-cyclic loading RTVs were recorded. RESULTS. Microscopic readings showed significant differences between frameworks. PF demonstrated the lowest measurements of 16.04 (2.6) ㎛ while CAD-cast demonstrated the highest measurements of 29.2 (3.1) ㎛. In all groups, RTVs were significantly lower than the applied torque. Post-cyclic loading RTV was significantly lower than pre-cyclic loading RTV in PF and 3DP frameworks. Differences in RTVs between the three manufacturing techniques were insignificant. CONCLUSION. Although CAD-cast and three-dimensionally printed (3DP) both produce frameworks with clinically acceptable misfit, 3DP might not be the technique of choice for maintaining screw's preload stability under an aggressive loading situation.

Enhanced Environmental Stability of Graphene Field-Effect Transistors through Interface Control (계면 제어를 통한 그래핀 기반 전계효과 트랜지스터의 환경 안정성 향상)

  • Seong, Jun Ho;Lee, Dong Hwa;Lee, Eunho
    • Journal of Adhesion and Interface
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    • v.23 no.3
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    • pp.75-79
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    • 2022
  • Graphene is a two-dimensional carbon allotrope composed of honeycomb sp2 hybrid orbital bonds. It shows excellent electrical and mechanical properties and has been spotlighted as a core material for next-generation electronic devices. However, it exhibits low environmental stability due to the easy penetration or adsorption of external impurities from the formation of an unstable interface between the materials in the electronic devices. Therefore, this work aims to improve and investigate the low environmental stability of graphene-based field-effect transistors through direct growth using solid hydrocarbons as a precursor of graphene. Graphene synthesized from direct growth shows high electrical stability through reduction of change in charge mobility and Dirac voltage. Through this, a new approach to utilize graphene as a core material for next-generation electronic devices is presented.