• 제목/요약/키워드: Digital holographic microscopy

검색결과 26건 처리시간 0.031초

Three Dimensional Shape Measurement of a Micro Fresnel Lens with In-line Phase-shifting Digital Holographic Microscopy

  • Kang, Jeon-Woong;Hong, Chung-Ki
    • Journal of the Optical Society of Korea
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    • 제10권4호
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    • pp.178-183
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    • 2006
  • An in-line phase-shifting digital holographic microscopy system was constructed by inserting a conventional microscope in the object arm of a Mach-Zehnder interferometer. It was used to measure the three dimensional shape of a micro Fresnel lens. It was also shown that both the lateral and the axial resolutions of the in-line phase-shifting system using a self-calibration algorithm were superior to those of the best off-axis system.

Optical Scanning Holography - A Review of Recent Progress

  • Poon, Ting-Chung
    • Journal of the Optical Society of Korea
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    • 제13권4호
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    • pp.406-415
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    • 2009
  • Optical scanning holography (OSH) is a distinct digital holographic technique in that real-time holographic recording a three-dimensional (3-D) object can be acquired by using two-dimensional active optical heterodyne scanning. Applications of the technique so far have included optical scanning cryptography, optical scanning microscopy, 3-D pattern recognition, 3-D holographic TV, and 3-D optical remote sensing. This paper reviews some of the recent progress in OSH. Some possible further works are also discussed.

Phase Differences Averaging (PDA) Method for Reducing the Phase Error in Digital Holographic Microscopy (DHM)

  • Hyun-Woo, Kim;Jaehoon, Lee;Arun, Anand;Myungjin, Cho;Min-Chul, Lee
    • Journal of information and communication convergence engineering
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    • 제21권1호
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    • pp.90-97
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    • 2023
  • Digital holographic microscopy (DHM) is a three-dimensional (3D) imaging technique that uses the phase information of coherent light. In the reconstruction process of DHM, a narrow region around the positive or negative sideband from the Fourier domain is windowed to avoid noise due to the DC spectrum of the hologram spectrum. However, the limited size of the window also degrades the high-frequency information of the 3D object profile. Although a large window can have more detailed information of the 3D object shape, the noise is increased. To solve this trade-off, we propose phase difference averaging (PDA). The proposed method yields high-frequency information of the specimen while reducing the DC noise. In this paper, we explain the reconstruction algorithm for this method and compare it to various conventional filtering methods including Gaussian, Wiener, average, median, and bilateral filtering methods.

Noise reduction method using a variance map of the phase differences in digital holographic microscopy

  • Hyun-Woo Kim;Myungjin Cho;Min-Chul Lee
    • ETRI Journal
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    • 제45권1호
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    • pp.131-137
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    • 2023
  • The phase reconstruction process in digital holographic microscopy involves a trade-off between the phase error and the high-spatial-frequency components. In this reconstruction process, if the narrow region of the sideband is windowed in the Fourier domain, the phase error from the DC component will be reduced, but the high-spatial-frequency components will be lost. However, if the wide region is windowed, the 3D profile will include the high-spatial-frequency components, but the phase error will increase. To solve this trade-off, we propose the high-variance pixel averaging method, which uses the variance map of the reconstructed depth profiles of the windowed sidebands of different sizes in the Fourier domain to classify the phase error and the high-spatial-frequency components. Our proposed method calculates the average of the high-variance pixels because they include the noise from the DC component. In addition, for the nonaveraged pixels, the reconstructed phase data created by the spatial frequency components of the widest window are used to include the high-spatialfrequency components. We explain the mathematical algorithm of our proposed method and compare it with conventional methods to verify its advantages.

Autofocus Tracking System Based on Digital Holographic Microscopy and Electrically Tunable Lens

  • Kim, Ju Wan;Lee, Byeong Ha
    • Current Optics and Photonics
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    • 제3권1호
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    • pp.27-32
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    • 2019
  • We present an autofocus tracking system implemented by the digital refocusing of digital holographic microscopy (DHM) and the tunability of an electrically tunable lens (ETL). Once the defocusing distance of an image is calculated with the DHM, then the focal plane of the imaging system is optically tuned so that it always gives a well-focused image regardless of the object location. The accuracy of the focus is evaluated by calculating the contrast of refocused images. The DHM is performed in an off-axis holographic configuration, and the ETL performs the focal plane tuning. With this proposed system, we can easily track down the object drifting along the depth direction without using any physical scanning. In addition, the proposed system can simultaneously obtain the digital hologram and the optical image by using the RGB channels of a color camera. In our experiment, the digital hologram is obtained by using the red channel and the optical image is obtained by the blue channel of the same camera at the same time. This technique is expected to find a good application in the long-term imaging of various floating cells.

액체와 Digital Holographic Microscopy을 이용한 비구면 렌즈 측정 연구 (Aspheric Lens Measurements by Digital Holographic Microscopy and Liquid)

  • 김두철;신상훈;유영훈
    • 한국광학회지
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    • 제24권6호
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    • pp.318-323
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    • 2013
  • 디지털 홀로그래피 현미경은 기준광과 시료의 굴절률과 두께에 의한 광경로 차이를 간섭 방법을 이용하여 측정하여 3차원 형상을 측정하는 방법이다. 그리고 광 경로차는 시료의 두께와 시료와 시료 주변의 굴절률 차이에 의존한다. 시료 형상 기울기가 매우 큰 경우에는 광 경로차가 커져 일반적인 간섭 방법으로는 측정이 어렵다. 이런 경우 시료를 액체에 담그어 측정하면 광 경로차가 매우 작아져서 일반적인 간섭 방법을 이용하여 측정할 수 있다. 본 연구에서는 디지털 홀로그램 현미경과 액체를 이용하여 기울기가 큰 비구면 렌즈의 형상을 측정하는 연구를 하였다. 시료의 굴절률과 비슷한 액체를 선택함으로서 비구면 렌즈의 정밀한 3차원 측정이 가능하였다.

A Phase Unwrapping Algorithm in Three-Wavelength Digital Holographic Microscopy

  • Shin, Sanghoon;Yu, Younghun
    • 새물리
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    • 제68권12호
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    • pp.1378-1383
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    • 2018
  • Multi-wavelength holography has a better axial range than single-wavelength holography, thus allowing unambiguous phase imaging. Noise amplification is the limiting factor in multi-wavelength holography as noise is amplified by a factor equivalent to the magnification of the wavelengths. Here, we propose a new algorithm to remove noise amplification in multi-wavelength holography. The proposed method does not use phase unwrapping and removes $2{\pi}$ ambiguities. Experiments and numerical simulations indicated that the proposed method is fast, has low noise, and is useful for measuring samples with arbitrary step heights.