• Korean Journal of Ecology and Environment
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• v.52 no.4
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• pp.394-402
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• 2019

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

• Journal of Internet Computing and Services
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• v.7 no.3
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• pp.107-118
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• 2006

Recently, network infra grows rapidly and the digital image compression technique have made remarkable progress, and therefore the demand of the real-time image surveillance system which uses a network camera server is increasing. Network Camera Server has emerged as an attractive alternative to the CCTV for the real-time image surveillance. From this reason, the demand regarding the development of the real-time image surveillance system which uses a network camera server is increasing as well. In this paper, we will provide a model for JPEG Streamer which is used in real-time image surveillance system. And we will design and implement this model. For the stability and efficiency of the JPEG Streamer, we'll use the thread pool and shared memory. We will also introduce the concept of double buffering for increasing the quality of real-time image, Using the JPEG Streamer, a raising of the productivity, reliability and stability will be able to secure to development of real-time image surveillance system.

• Journal of Digital Convergence
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• v.12 no.12
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• pp.537-544
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• 2014

The film has far-reaching power than any other media. For this reason, a censorship has existed in some way but censorship is like a double-edged sword. If KMRB stood in front of a desire to preserve the morals of society and youth protection, creativity of artists must be shaken. On the contrary if free will is emphasized, social chaos will be increase. Thus most countries made various censorship systems in order to overcome this contradiction. In recent years, mainly on the issue of censorship and the rating is a tendency to transfer to the voluntary deliberation agency. However, this issue is still dealing with this problem at KMRB in Korea. Then KMRB mistakes itself as the guardian of situation ethics and morality. Recently various problems appeared in the film rating system. KMRB should be devolved on the voluntary deliberation agency in order to solve the problem. therefore this study The study will be to review the need for voluntary veliberation agency.

• Journal of Digital Convergence
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• v.12 no.6
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• pp.311-316
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• 2014

Social network server due to the popularity of smart phones, and data stored in a big usable access data services are increasing. Big Data Big Data processing technology is one of the most important technologies in the service, but a solution to this minor security state. In this paper, the data services provided by the big -sized data is distributed using a double hash user to easily access to data of multiple distributed hash chain based data processing technique is proposed. The proposed method is a kind of big data data, a function, characteristics of the hash chain tied to a high-throughput data are supported. Further, the token and the data node to an eavesdropper that occurs when the security vulnerability to the data attribute information to the connection information by utilizing hash chain of big data access control in a distributed processing.

• Journal of Digital Convergence
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• v.15 no.10
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• pp.513-524
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• 2017

This study proposed an 'Crime Victim Care Specialist(CVCS)' convergence curriculum for psychology and police students in college. First, the courses and the curriculums of counseling, psychology, psychotherapy, police, and police administration departments in nationwide were listed. After consulting with professors from 2 majors, 3-levels curriculum and courses were drafted. Second, for the inputs from the field, a panel of 51 crime victim care/counseling/psychotherapy experts were Delphi surveyed about goodness of fit and importance. The result were following. First, the curriculum were consisted of required, basics, advanced courses. The required course were 5 courses for each department. The basics were for the minors and 7 courses for each. The advanced were for the double majors and 4 courses for each. Finally, the implication and further studies were suggested.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.36D no.1
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• pp.63-73
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• 1999

This paper presents new pipeline architecure for low power and high speed digital FIR filters. The proposed architecture based on retiming technique achieves enhancement on speed by sharing the input delay stage with multiplication of input data and on power combined with supply voltage scaling down technique. An 8-tap digital FIR filter for PRML disk-drive read channels adopting the proposed pipeline architecture has been designed and fabricated with 0.8${\mu}m$ CMOS double metal process technology. Measured results show that the designed FIR filter operates to 192 MHz in average and dissipates 1.22 mW/MHz at 3.3.V power supply. As a result, the proposed architecture improves speed by about 16% and reduces power dissipation by about 23% when operating at the same throughput.

• Journal of the Korean Ceramic Society
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• v.43 no.1 s.284
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• pp.10-15
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• 2006

Photoluminescence (PL) and crystal structures of the $(l-x)CaWO_4-xLi_2WO_4$ binary system added with $Eu_2O_3$ activator have been characterized. The $CaWO_4\;and\;Li_2WO_4$ have the scheelite and phenakite structures respectively. The $CaWO_4-Li_2WO_4-Eu_2O_3$ phosphors show the red luminescence of 613 nm peak wavelength. The wavelength range of excitation spectral band is $380\~470$ nm with the peak wavelength of 397 nm. The $0.88(0.5CaWO_4-0.5Li_2WO_4)-0.12Eu_2O_3$ showed the most superior luminescence characteristics. The effect of co-doping elements such as $Al_2O_3$ and rare-earth oxides on PL has been characterized. The co-doping elements deteriorated the luminescence intensity except the $Al_2O_3$ and $Gd_2O_3$. The PL characteristics of $CaWO_4-Li_2WO_4-Eu_2O_3$ phosphors have been compared to those of the alkali europium double molybdates (tungstates) of scheelite-related structure such as $LiEu(MoO_4)_2$ and $CsEu(MoO_4)_2$. The crystal structures of $(l-y)[(l-x)CaWO_4-xLi_2WO_4]-yEu_2O_3$ phosphors have been characterized using XRD data and rietveld refinement.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.11
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• pp.61-66
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• 2005

This paper describes a 10-bit 210MHz CMOS current-mode Digital-to-Analog Converter (DAC) consisting of 6 bit MSB current cell matrix Sub-DAC, 2 bit mSB unary current source Sub-DAC, and 2 bit LSB binary weighting Sub-DAC for Wireless LAN application. A new deglitch circuit is proposed to control a crossing point of signals and minimize a glitch energy. The proposed 10-bit CMOS current mode DAC was designed by a $0.35{\mu}m$ CMOS double-poly four-metal technology rate of 210MHz, DNL/INL of ${\pm}0.7LSB/{\pm}1.1LSB$, a glitch energy of $76pV{\cdot}sec$, a SNR of 50dB, a SFDR of 53dB at 200MHz sampling clock and power dissipation of 83mW at 3.3V

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.44 no.1
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• pp.1-11
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• 2007

In this paper, a new fully digital control method for UPS inverter, which is based on the double control loop such as the outer voltage control loop and inner current control loop, is proposed. In the proposed control system, overshoots and oscillations due to the computation time-delay are compensated by explicit incorporation of the time-delay in the current control loop transfer function. The inner current control loop is adopted by an internal model controller. The internal model controller is designed to a second order deadbeat reference-to-output response which means that its response reaches the reference in two sampling time including computational time-delays. The outer voltage control loop employing P-Resonance controller is proposed. The resonance controller has an infinite gain at resonant frequency, and the resonant frequency is set to the fundamental frequency of the reference voltage in this paper. Thus the outer voltage control loop causes no steady state error as regard to both magnitude and phase. The effectiveness of the proposed control system has been verified by the simulation and experimental results respectively.

• The Transactions of the Korean Institute of Power Electronics
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• v.6 no.2
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• pp.202-208
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• 2001

A Switched Reluctance Motor(SRM) has double salient poles structure and the phase windings are wound in stator. SRM hase more simple structure that of other motor, thus manufacture cost is low, mechanically strong, reliable to a poor environment such as high temperature, and maintenance cost is low because of brushless. SRM needs position detector to get rotator position information for phase excitation and tachometer or encoder for constant speed operation. But, this paper doesn\`s use an encoder of high cost for velocity measurement of rotator. Instead of it, the algorithm for position detection and velocity estimation from simple slotted disk has been proposed and developed. To implement variable speed digital control system with velocity estimation algorithm, the TMS320F240-20MIPS fixed point arithmetic processor of TI corporation is used. The experimental results of the developing system are enable to control speed with wide range, not only single pulse, hard chopping mode and soft chopping, ut also variable speed control, and advance angle control.