• 디지털콘텐츠학회 논문지
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• 제18권1호
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• pp.209-215
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• 2017

이모티콘 캐릭터는 모바일 공간에서 상대와 대화하는 가운데 대화내용과 이미지를 혼용하여 사용함으로써 디지털 시대의 표현성에 적합하다. 이러한 관점에서 모바일 콘텐츠 산업의 시장 확장에 이모티콘 캐릭터의 역할은 중요한 부분을 차치한다. 본 연구에서는 이러한 맥락에서 최근 산업적으로 급성장하고 있는 모바일메신저 유료 이모티콘의 캐릭터 속성이 구매의도에 어떠한 영향을 미치는가를 분석함으로써 소비자구매형태를 확인하는 것이 그 목적이다. 이를 위해 독립변인을 캐릭터 속성요인인 인지성, 친근성, 자아표현성, 이미지차별성으로, 종속변인은 유료 구매자의 구매의도로 하여 다중회귀분석을 실시하였다. 분석결과, 인지성과 이미지차별성은 구매의도에 긍정적 영향을 미치는 것으로 나타났고, 친근성과 자아표현성은 구매의도에 유의미한 영향력이 없는 것으로 나타났다.

• 대한한의진단학회지
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• 제16권1호
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• pp.27-34
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• 2012

Objectives The purpose of this study was to find out whether if there is correlation between the pattern differentiation questionnaire and edema index of body composition analysis. Methods The authors obtained results of pattern differentiation Questionnaires and body composition analysis tests from 195 female patients who visited the Oriental gynecology department in Kyung Hee university Oriental medicine hospital at Gang-dong. The authors conducted correlation analysis between indicators of Body composition analysis and each part of the pattern differentiation Questionnaire. Pearson correlation analysis was performed on SPSS version 13.0 for windows. Results There was negative correlation between the Phlegm Questionnaire and right arm ECF, left arm ECF, and left arm ECW of the Body composition index. There was no correlation between Yin-deficiency Questionnaire and Body composition index. There was no correlation between Heat pattern Questionnaire and Body composition index. There was positive correlation between the Cold pattern Questionnaire and right leg ECF, and right leg ECW of the body composition index. There was no correlation between degree of edema self-awareness and Body composition index. Conclusions The result showed that each Questionnaire score has different correlation or even no correlation with Body composition index and degree of edema self-awareness. Further studies are needed for a better understanding and interpretation of the relationship between edema index on Body composition analysis and Questionnaires.

• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.25-30
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• 2011

Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF-${\kappa}B$ is a transcription factor involved in many biological activities. Expression and activity of NF-${\kappa}B$ increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF-${\kappa}B$ protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF-${\kappa}B$ in the human Nanog promoter and postulated that NF-${\kappa}B$ protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF-${\kappa}B$ upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF-${\kappa}B$ binding site suggested involvement of NF-${\kappa}B$ in the negative regulation of the promoter, site-directed mutation of NF-${\kappa}B$ binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF-${\kappa}B$ with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF-${\kappa}B$ protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.

• Journal of Ecology and Environment
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• 제45권3호
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• pp.97-104
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• 2021

Background: Asarum sieboldii Miq., a species of forest understory vegetation, is an herbaceous perennial belonging to the family Aristolochiaceae. The metapopulation of A. sieboldii is distributed sparsely and has a short seed dispersal distance by ants as their seed distributor. It is known that many flowers of A. sieboldii depend on self-fertilization. Because these characteristics can affect negatively in genetic structure, investigating habitat structure and assessment of genetic structure is needed. A total of 27 individuals in a valley were sampled for measuring genetic diversity, genetic distance, and genetic differentiation by RAPDPCR. Results: The habitat areas of A. sieboldii metapopulation were relatively small (3.78~33.60 m²) and population density was very low (five to seven individuals in 20×20 m quadrat). The habitat of A. sieboldii was a very shady (relative light intensity = 0.9%) and mature forest with a high evenness value (J = 0.81~0.99) and a low dominance value (D = 0.19~0.28). The total genetic diversity of A. sieboldii was quite high (h = 0.338, I = 0.506). A total of 33 band loci were observed in five selected primers, and 31 band loci (94%) were polymorphic. However, genetic differentiation along the valley was highly progressed (G_st = 0.548, N_m = 0.412). The average genetic distance between subpopulations was 0.387. The results of AMOVA showed 52.77% of variance occurs among populations, which is evidence of population structuring. Conclusions: It is expected that a small-scale founder effect had occurred, an individual spread far from the original subpopulation formed a new subpopulation. However, geographical distance between individuals would have been far and genetic flow occurred only within each subpopulation because of the low density of population. This made significant genetic distance between the original and new population by distance. Although genetic diversity of A. sieboldii metapopulation is not as low as concerned, the subpopulation of A. sieboldii can disappear by stochastic events due to small subpopulation size and low density of population. To prevent genetic isolation and to enhance the stable population size, conservative efforts such as increasing the size of each subpopulation or the connection between subpopulations are needed.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6409-6413
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• 2012

The innate immune system coordinates the inflammatory response to pathogens. To do so, its cells must discriminate self from non-self utilizing receptors that identify molecules synthesized exclusively by microbes. Toll-like receptors have a crucial role in the detection of microbial infection in mammals and insects. In mammals, they have evolved to recognize conserved products unique to microbial metabolism. These include lipopolysaccharide (LPS), lipotechoic acids, and peptidoglycans (PGN). We show here that TLRs, including TLR2, are expressed on the THP-1 human leukemia cell line. Activation of TLR2 signaling in THP-1 by PGN induces the synthesis of various soluble factors and proteins including interleukin-$1{\beta}$, interleukin-8 and TNF-${\alpha}$ and apoptosis of THP-1 with PGN dose and time dependence. Moreover, in this study we show that PGN induces apoptosis of THP-1 cells in a TNF-${\alpha}$-dependent manner. These findings indicate that TLR2 signaling results in a cascade leading to tumor apoptosis and differentiation, which may suggest new clinical prospects using TLR2 agonists as cytotoxic agents in certain cancers.

• 한국간호교육학회지
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• 제21권4호
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• pp.509-517
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• 2015

Purpose: This study examined diabetes knowledge, self-efficacy, and health promoting behaviors, and investigated factors affecting health-promoting behaviors of middle-aged people. Methods: From August to September 2014, a convenience sample of 264 subjects was recruited from B city and G province. The data analysis was done by ${\chi}^2$ test, t-test, Pearson's correlation coefficient, and stepwise multiple regression. Results: Diabetes knowledge was $11.3{\pm}3.63$ in men and $12.7{\pm}3.74$ in women. The average levels of self-efficacy and health promoting behaviors of subjects were similar with other middle-aged individuals. Diabetes knowledge, self-efficacy, and health promoting behaviors had a positive correlation with each other in both men and women. To test the mediating effect of self-efficacy in the relationship between diabetes knowledge and health promoting behaviors, a Sobel test was performed and Z-scores of 3.698(p<.001) in men and 2.748(p=.006) in women were obtained. Conclusion: Diabetes prevention education is recommended for the middle-aged community. When developing diabetes prevention program for middle-aged people, such programs should consider self-efficacy, especially in women.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권6호
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• pp.588-593
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• 2008

There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and $PPAR{\gamma}2$ and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.

• Molecules and Cells
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• 제46권12호
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• pp.746-756
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• 2023

A recent study revealed that the loss of Deup1 expression does not affect either centriole amplification or multicilia formation. Therefore, the deuterosome per se is not a platform for amplification of centrioles. In this study, we examine whether gain-of-function of Deup1 affects the development of multiciliated ependymal cells. Our time-lapse study reveals that deuterosomes with an average diameter of 300 nm have two different fates during ependymal differentiation. In the first instance, deuterosomes are scattered and gradually disappear as cells become multiciliated. In the second instance, deuterosomes self-organize into a larger aggregate, called a deuterosome cluster (DC). Unlike scattered deuterosomes, DCs possess centriole components primarily within their large structure. A characteristic of DC-containing cells is that they tend to become primary ciliated rather than multiciliated. Our in utero electroporation study shows that DCs in ependymal tissue are mostly observed at early postnatal stages, but are scarce at late postnatal stages, suggesting the presence of DC antagonists within the differentiating cells. Importantly, from our bead flow assay, ectopic expression of Deup1 significantly impairs cerebrospinal fluid flow. Furthermore, we show that expression of mouse Deup1 in Xenopus embryos has an inhibitory effect on differentiation of multiciliated cells in the epidermis. Taken together, we conclude that the DC formation of Deup1 in multiciliated cells inhibits production of multiple centrioles.

• BMB Reports
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• 제39권4호
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• pp.426-431
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• 2006

Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution. Enzymes were extracted by sonication and centrifugation for kinetic evaluation of LDH and CK with spectrophotometric methods. While a comparison between the kinetic properties of the LDH and CK of both groups revealed not only different Michaelis constants and optimum temperatures for LDH but also different Michaelis constants and optimum pH for CK, the pH profile of LDH and optimum temperature of CK were similar. In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model.

• 한국동물생명공학회지
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• 제34권3호
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• pp.139-147
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• 2019

Stem cells are progenitor cells that are capable of self-renewal and differentiation into various cells. Especially, pluripotent stem cells (PSCs) have in vivo and in vitro differentiation capacity into three germ layers and can proliferate infinitely. The differentiation ability of PSCs can be applied for regenerative medicine and tissue engineering. In domestic animals, their PSCs have a potential for preclinical therapy as well as the production of transgenic animals and agricultural usage such as cultured meat. Among several domestic animals, a pig is considered as an ideal model for biomedical and agricultural purposes mentioned above. In this reason, studies for pig PSCs including embryonic stem cells (ESCs), embryonic germ cells (EGCs) and induced pluripotent stem cells (iPSCs) have been conducted for decades. Therefore, this review will discuss the history of PSCs derived from various origins and recent progress in pig PSC research field.