• Molecular & Cellular Toxicology
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• 제2권3호
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• pp.202-211
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• 2006

Our objective is to identify molecular factors which contribute to the increased risk of smoke in human. About 677 workers who had control and experimental groups according to their urinary Naphthol levels were enrolled in our study. In the present study, we investigated the effects of smoking on gene expression profiles in human. We determined differential gene expression patterns in smoker versus non-smoker using cDNA microarray. Specific genes were up-or down-regulated according to smoking and age. Inflammatory related genes such as cytokine, interleukin, and tumor necrosis factor were up-regulated, DNA repair related genes such as high-mobility group (nonhistone chromosomal) protein 1, and protein 2 were down-regulated, apoptosis related genes such as myeloperoxidase and Bcl-2-associated athanogene were down-regulated, and cell cycle related genes were down-regulated. In our epidemiological study, notably, inflammatory, DNA repair, apoptosis, signal transduction, metabolism, cell cycle, cell proliferation, transcription related genes were regulated.

• Journal of Microbiology
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• 제44권4호
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• pp.369-374
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• 2006

To investigate the mechanism of salt tolerance of gram-positive moderately halophilic bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis $D-8^T$. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain $D-8^T$ are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and $Imagemaster^{TM}$ 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, S proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.

• 대한의용생체공학회:의공학회지
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• 제34권1호
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• pp.24-33
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• 2013

To enhance the speech perception of hearing aid users in noisy environment, most hearing aid devices adopt various beamforming algorithms such as the first-order differential microphone (DM1) and the two-stage directional microphone (DM2) algorithms that maintain sounds from the direction of the interlocutor and reduce the ambient sounds from the other directions. However, these conventional algorithms represent poor directionality ability in low frequency area. Therefore, to enhance the speech perception of hearing aid uses in low frequency range, our group had suggested a fractional delay subtraction and integration (FDSI) algorithm and estimated its theoretical performance using computer simulation in previous article. In this study, we performed a KEMAR test in non-reverberant room that compares the performance of DM1, DM2, broadband beamforming (BBF), and proposed FDSI algorithms using several objective indices such as a signal-to-noise ratio (SNR) improvement, a segmental SNR (seg-SNR) improvement, a perceptual evaluation of speech quality (PESQ), and an Itakura-Saito measure (IS). Experimental results showed that the performance of the FDSI algorithm was -3.26-7.16 dB in SNR improvement, -1.94-5.41 dB in segSNR improvement, 1.49-2.79 in PESQ, and 0.79-3.59 in IS, which demonstrated that the FDSI algorithm showed the highest improvement of SNR and segSNR, and the lowest IS. We believe that the proposed FDSI algorithm has a potential as a beamformer for digital hearing aid devices.

• 대한전자공학회논문지SD
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• 제38권7호
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• pp.493-502
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• 2001

기존의 논리 테스팅에 비하여 여러 가지 장점을 가지는 전류 테스팅을 위하여 새로운 내장형 전류 감지 회로를 설계하였다. 본 논문에서 제안된 내장형 전류 감지 회로는 시험 대상 회로에서 발생하는 전류와 인버터의 전류 발생 특성에 의해 복사되어진 전류를 비교함으로서 시험 대상 회로의 고장 존재 여부를 감지하여 Pass/Fail 신호로 발생시킨다. 설계된 회로는 차동 증폭 형태의 증폭기와 비교기로 이루어져 있으며, 시험 대상 회로의 전류를 복사해 내기 위한 인버터를 포함하고 있어서 총 10개의 트랜지스터와 3개의 인버터를 사용한다. 본 논문에서 제안된 내장형 전류 감지 회로는 고장 테스트를 위하여 별도의 클럭을 사용하지 않는다. 또한 모드 선택이 필요하지 않아 on-line 테스팅이 가능하며, Pass/Fail 신호를 칩의 외부로 전달하는 출력단자 하나를 제외하고는 별도의 제어단자가 필요하지 않은 장점을 가진다. HSPICE를 사용한 컴퓨터 모의 실험을 통하여 시험 대상 회로에 삽입된 고장을 정확하게 검출해 낼 수 있음을 확인하였다. 제안된 내장형 전류 감지 회로가 칩의 전체 면적에서 차지하는 면적소모는 8×8 병렬 승산기를 시험 대상 회로로 사용한 경우에 약 4.34 %로 매우 작아서 내장형 전류 감지회로에 의한 면적 소모에 대한 부담은 거의 없는 것으로 측정되었다.

• BMB Reports
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• 제33권6호
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• pp.493-498
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• 2000

Aquifex pyrophilus, a hyperthermophilic bacterium, has a serine-type protease that is located at the cell wall fraction with a mature size of 43 kDa. Molecular cloning of the protease gene revealed that it has an ORF of 619 amino acids with homologous catalytic site of serine-type proteases [Choi, I.-G., Bang, W.-K., Kim, S.-H., Yu, G. Y., J. Biol. Chem. (1999), Vol. 274, pp. 881-888]. Constructs containing different regions of the protease gene, including a alanine-substituted mutant at the active site serine, were constructed, and the factors affecting the expression level of the cloned protease gene in E. coli were examined. The presence of the C-terminus hydrophobic region of the protease hindered over-expression in E. coli. Also, the proteolytic activity of the expressed protein appeared to toxic to E. coli. An inactive form that deleted both of the N-terminal signal sequence and the C-terminal polar residues was over-expressed in a soluble form, purified to homogeneity, and its thermostability examined. The purified protein showed three disulfide bonds and three free sulfhydryl group. The thermal denaturation temperature of the protein was measured around $90^{\circ}C$ using a differential scanning calorimeter and circular dichroism spectrometry. The disulfide bonds were hardly reduced in the presence of reducing agents, suggesting that these disulfide bonds were located inside of the protein surface.

• Investigative Magnetic Resonance Imaging
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• 제13권1호
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• pp.88-92
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• 2009

과립세포육종은 골수모세포라 불리는 미성숙 악성 백혈구로 구성된 골수 외 종양이다. 과립세포육종은 보통 급성골수성 백혈병이나 골수이형성 증후군과 동반되나 드물게 급성 골수성 백혈병의 전신 질환 이전에 초기 발현으로 발병하기도 한다. 저자의 증례에서와 같이 급성 골수성 백혈병 확진 이전의 과립세포육종은 영상 소견이 임상적 의심과 함께 이 질환의 진단과 치료에 중요하다. 이 증례에서 출혈성 농양과 오인 가능한 과립세포육종의 자기공명영상 소견을 보고하고자 한다. 저자는 자기공명영상이 급성 골수성 백혈병 환자에서 농양으로부터 과립세포육종을 감별하는데 도움이 된다고 생각한다.

• 한국동물학회지
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• 제39권4호
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• pp.410-418
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• 1996

Gangiloside는 포유동물세포에 편재하는 막성분으로서, 이들은 세포상호간의 접착, 분화 및 정보전달과정에 과여하는 것으로 알려지고 있다. Rat난소는 주요한 Gangiloside로서 GM3를 함유하고 있으며, 본 연구에서는 폐쇄난포에서 이들의 분포여부와 난포의 발달 과정에서의 변화여부를 조사하기 위하여, Rat 난소의 동결절편을 이용해 GM3를 포함 11종류의 Gangilo-series Gangiloside에 대해 특이한 단일항체로서 염색시켰다. 폐쇄난포들에서 GM3는 난포발달이 진행되는 동안 시간적, 공간적으로 서로 다른 양식으로 발현하였다. 그러나 GM1을 포함한 다른 종류의 Gangiloside들은 면역조직화학적으로 검출되지 않았다. 일차난포에서 관찰되는 폐쇄난포들에서 GM3는 모든 교막세포와 난자에 인접한 과립막세포의 일부에서 발현하였다. 이차난포의 시기에서 이들 폐쇄난포의 GM3는 모든 교막세포와 과립막세포들에서 발현하였다. 이어서 발달하고 있는 그라프난포의 시기에서 관찰되는 폐쇄난포의 GM3발현은 이차난포에서의 분포패턴과 유사함을 보여 주었다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제11권2호
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• pp.95-103
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• 2011

This paper proposes a 6b 1.2 GS/s 47.8 mW 0.17 $mm^2$ 65 nm CMOS ADC for high-rate wireless personal area network systems. The proposed ADC employs a source follower-free flash architecture with a wide input range of 1.0 $V_{p-p}$ at a 1.2 V supply voltage to minimize power consumption and high comparator offset effects in a nanometer CMOS technology. The track-and-hold circuits without source followers, the differential difference amplifiers with active loads in pre-amps, and the output averaging layout scheme properly handle a wide-range input signal with low distortion. The interpolation scheme halves the required number of pre-amps while three-stage cascaded latches implement a skew-free GS/s operation. The two-step bubble correction logic removes a maximum of three consecutive bubble code errors. The prototype ADC in a 65 nm CMOS demonstrates a measured DNL and INL within 0.77 LSB and 0.98 LSB, respectively. The ADC shows a maximum SNDR of 33.2 dB and a maximum SFDR of 44.7 dB at 1.2 GS/s. The ADC with an active die area of 0.17 $mm^2$ consumes 47.8 mW at 1.2 V and 1.2 GS/s.

• JSTS:Journal of Semiconductor Technology and Science
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• 제13권2호
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• pp.98-107
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• 2013

This work describes a 13b 100 MS/s 0.13 um CMOS four-stage pipeline ADC for 3G communication systems. The proposed SHA-free ADC employs a range-scaling technique based on switched-capacitor circuits to properly handle a wide input range of $2V_{P-P}$ using a single on-chip reference of $1V_{P-P}$. The proposed range scaling makes the reference buffers keep a sufficient voltage headroom and doubles the offset tolerance of a latched comparator in the flash ADC1 with a doubled input range. A two-step reference selection technique in the back-end 5b flash ADC reduces both power dissipation and chip area by 50%. The prototype ADC in a 0.13 um CMOS demonstrates the measured differential and integral nonlinearities within 0.57 LSB and 0.99 LSB, respectively. The ADC shows a maximum signal-to-noise-and-distortion ratio of 64.6 dB and a maximum spurious-free dynamic range of 74.0 dB at 100 MS/s, respectively. The ADC with an active die area of 1.2 $mm^2$ consumes 145.6 mW including high-speed reference buffers and 91 mW excluding buffers at 100 MS/s and a 1.3 V supply voltage.

• 전기전자학회논문지
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• 제21권3호
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• pp.248-251
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• 2017

본 논문에서는 $360^{\circ}$ 전방위 화각을 가진 Dash Camera의 EMI 대응을 위한 보드 설계를 제안한다. 제안된 보드는 DM 및 CM 입력 노이즈 감소회로를 설계하고 능동 EMI 필터 결합회로를 적용하여 개발한다. DM 및 CM 입력 노이즈 감소회로 설계부분에서는 기생적 커패시턴스(CP)을 통해 입력 신호로 커플링된 DM 잡음을 얻기 위한 차동연산 증폭기 회로를 사용한다. 능동 EMI 필터 결합회로를 적용하여 설계하는 부분에서는 회로의 간략화를 위하여 EMI 소스의 노이즈를 분리하여 보상해 주기 위하여 노이즈 분리기를 설치하여 CM과 DM 능동필터를 동시에 보상한다. 제안된 EMI 대응을 위한 보드의 성능을 평가하기 위하여 공인 인정기관에서 실험한 결과, 각각의 주파수 대역에 따른 전자파 인증규격이 만족됨을 확인하였다.