• Biomolecules & Therapeutics
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• 제26권3호
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• pp.268-273
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• 2018

Sleep is the most basic and essential physiological requirement for mental health, and sleep disorders pose potential risks of metabolic and neurodegenerative diseases. Tryptic hydrolysate of ${\alpha}_{S1}$-casein (${\alpha}_{S1}-CH$) has been shown to possess stress relieving and sleep promoting effects. However, the differential effects of ${\alpha}_{S1}-CH$ on electroencephalographic wave patterns and its effects on the protein levels of ${\gamma}$-aminobutyric acid A ($GABA_A$) receptor subtypes in hypothalamic neurons are not well understood. We found ${\alpha}_{S1}-CH$ (120, 240 mg/kg) increased sleep duration in mice and reduced sleep-wake cycle numbers in rats. While ${\alpha}_{S1}-CH$ (300 mg/kg) increased total sleeping time in rats, it significantly decreased wakefulness. In addition, electroencephalographic theta (${\theta}$) power densities were increased whereas alpha (${\alpha}$) power densities were decreased by ${\alpha}_{S1}-CH$ (300 mg/kg) during sleep-wake cycles. Furthermore, protein expressions of $GABA_A$ receptor ${\beta}_1$ subtypes were elevated in rat hypothalamus by ${\alpha}_{S1}-CH$. These results suggest ${\alpha}_{S1}-CH$, through $GABA_A$ receptor modulation, might be useful for treating sleep disorders.

• The Korean Journal of Physiology and Pharmacology
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• 제5권5호
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• pp.423-431
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• 2001

Some recent investigations revealed that vasodilatory action of adenosine is mainly not mediated by surface A2 receptor and suggested the existence of an intracellular action site. In the present study, we tried to differentiate intracellular from extracellular site of adenosine action in the regulation of coronary flow. In perfused rabbit hearts, concentration-response curve of coronary flow to exogenous adenosine was constructed in the presence or absence of dipyridamole, an inhibitor of transmembrane purine transport. Inhibition of cellular adenosine uptake by dipyridamole suppressed the increase of flow rate while enhancing the decrease in heart rate induced by exogenous adenosine. In another series of experiments, perfused rabbit hearts were subjected to energy deprivation in order to increase the production of endogenous adenosine. Energy deprivation along with dipyridamole administration resulted in higher coronary flow rate. Lower perfusate adenosine concentration was observed along with higher tissue adenosine content in this group. These results implied that coronary flow rate is determined not by interstitial adenosine concentration but by intracellular activity of adenosine. To confirm the effects of dypiridamole in vivo, direct measurement of interstitial adenosine concentration by mycrodialysis along with the assay of intracellular adenosine content was performed after intranenous dipyridamole administration. After dipyridamole infusion, intracellular adenosine content was markedly increased while interstitial adenosine concentration was not altered. In another series of experiments, the right shift of concentration-response curve of adenosine-induced vasodilation by 8-phenyltheophilline, a representative adenosine receptor antagonist, was mostly abolished by prior administration of prazosin, indicating that the influence of 8-PT on the adenosine action is not attributed to the inhibition of A2 receptor but related to the suppression of ${\alpha}-adrenoceptor$ activation. From these results, we concluded that adenosine acts intracellularly to regulate the coronary blood flow.

• 대한임상검사과학회지
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• 제40권1호
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• pp.31-40
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• 2008

Despite of the importance of the primordial follicle (PMF) recruitment, factors and mechanisms for process are poorly understood. To evaluate expression and role of the follicular transition from PMF to PMF/primary follicles (PMIF) in the present study, we evaluated expression of lats1, lats2, cyclin A1, and cyclin A2 mRNA and protein, and elucidated and role of lats1-cyclin A in the follicular transition from PMF to PRIF. To analysis of differential expression in PMF and PMIF, each stage follicles were collected by day1 and day5 of immuno-compromised rats (ICR) and analyzed by real-time PCR for the genes. For localization of mRNAs and proteins of the genes, in situ hybridization and immunohistochemistry were performed. We confirmed that the lats1, lats2, cyclin A1, and cyclin A2 mRNA were more expressed in PMF than PMIF. Localization of the four genes expression were observed in nuclei of oocytes from the arrested primordial, and in the surrounding granulosa cells of the growing follicles. The mRNA expressions were gradually decreased with follicular development. From immunohistochemistry studies, Cyclin A1 protein expression were observed in oocyte cytoplasmas of early stage follicles, while observed in granulose cells and oocyte nucleoli during growing follicles. This study suggested that the presence of lats gene family might perform negatively regulation of cell proliferation by modulation of the CDC2/Cyclin A complex activity. lats-cyclin A genes in oocytes of the early stage follicles might play a role in the meiotic cell cycle arrest of the primary oocytes at the primordial follicle stage as well as the follicular growth.

• The Plant Pathology Journal
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• 제32권6호
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• pp.552-562
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• 2016

Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

• 천문학회지
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• 제37권4호
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• pp.159-169
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• 2004

This is a proposal to probe local part of the interplanetary dust (IPD) cloud complex and retrieve mean volume emissivity of the local IPDs at mid-infrared wavelengths. This will be done by monitoring, with Infrared Camera (IRC) aboard the ASTRO-F, the annual modulation of the zodiacal emission. In pointing mode of the ASTRO-F mission the spacecraft can make attitude maneuvering over approximately ${\pm}1^{\circ}$ range centered at solar elongation $90^{\circ}$ in the ecliptic plane. The attitude maneuvering combined with high sensitivity of the IRC will provide us with a unique opportunity observationally to take derivatives of the zodiacal emission brightness with respect to the solar elongation. From the resulting differential of the brightness over the ${\pm}1^{\circ}$ range, one can directly determine the mean volume emissivity of the local IPDs with a sufficient accuracy to de-modulate the annual emissivity variations due to the Earth's elliptical motion and the dis-alignment of the maximum IPD density plane with respect to the ecliptic. The non-zero eccentricity ($e_{\oplus}$= 0.0167) of the Earth's orbit combined with the sensitive temperature dependence of the Planck function would bring modulations of amplitude at least $3.34\%$ to the zodiacal emission brightness at mid-infrared wavelengths, with which one may determine the IPD temperature T(r) and mean number density n(r) as functions of heliocentric distance r. This will in turn fix the power-law exponent $\delta$ in the relation $T(r) = T_o(r/r_o)^{-\delta}$ for the dust temperature and v in $n(r) = n_o(r/r_o)^-v$ for the density. We discuss how one may de-couple the notorious degeneracy of cross-section, density, reference temperature $T_o$ and exponent $\delta$.

• Archives of Pharmacal Research
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• 제23권5호
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• pp.518-524
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• 2000

We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-Bl(2) and rat glioma C6Bu-1 cells. Apoptosis induced by C-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation and in situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes $\alpha$, $\beta$ and $\gamma$ were progressively increased with prolonged treatment, whereas PKC $\delta$ increased transiently at 3 and 6 h and PKC $\varepsilon$ was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype $\beta$ markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-l cells, no significant changes in PKC subtypes $\alpha$, $\gamma$, $\delta$, $\varepsilon$ and $\beta$ were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types.

• Mycobiology
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• 제43권4호
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• pp.450-457
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• 2015

Medicinal mushrooms have been used worldwide to treat cancer and modulate the immune system. Over the last several years, there has been increasing interest in isolating bioactive compounds from medicinal mushrooms and evaluating their health beneficial effects. Fomes fomentarius is used in traditional oriental medicine and is known to possess antioxidant, antiinflammatory, antidiabetic, and antitumor effects. In the present study, we isolated fomentariol from Fomes fomentarius and investigated its anti-inflammatory effect in murine macrophages (RAW264.7 cells) stimulated with lipopolysaccharides. Fomentariol inhibited the production of nitric oxide and intracellular reactive oxygen species triggered by lipopolysaccharides. Interestingly, fomentariol differentially regulated cytokine production triggered by lipopolysaccharides. Fomentariol effectively suppressed the production of interleukin-$1{\beta}$ and interleukin-6 but not tumor necrosis factor-${\alpha}$. The inhibitory effect of fomentariol against nitric oxide, interleukin-$1{\beta}$, and interleukin-6 production was possibly mediated by downregulation of the extracellular signal-regulated kinase signaling pathway. Taken together, our results suggest that fomentariol differentially modulated inflammatory responses triggered by lipopolysaccharides in macrophages and is one of the bioactive compounds that mediate the physiological effects of Fomes fomentarius.

• 한국통신학회논문지
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• 제17권9호
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• pp.939-950
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• 1992

Collision-free 전송(傳送) 가능성과 2차 다중(多重) 접속 간섭(干涉)의 영향면에서 다중(多重) 캡쳐 수신기(受信機)를 사용한 단일확산(單一擴散)코드 방식의 성능(性能)이 송신기별(送信機別)(T/O) 코드분할 다중(多重)접속(CDMA) 방식(方式)의 성능(性能)과 비교(比較)된다. 성능비교(性能比較)를 위해, 단일(單一)코드 방식(方式)과 완전한 collision-free 전송(傳送)을 보장하는 T/O CDMA 방식(方式)에 대해 다중(多重)접속 간섭(干涉)의 특징이 서술(敍述)된다. 백색(白色) 가우시안 잡음(雜音)하에서 직접(直接)시퀀스 차동(差動) 위상(位相)편이키잉 데이터 변조(變調)와 forward 에러정정부호화(訂正符號化)를 (BCH 코드) 사용한 위의 두방식(方式)에 대해 throughput 성능(性能)이 평가(評價)된다. 라디오의 수(數)가 비교적 많은 경우에, 적당한 신호대(信號對) 잡음화(SNR)에서 단일(單一)코드 방식(方式)이 T/O CDMA방식보다 최대(最大) throughput을 증가(增加)시킬 수 있음을 확인(確認)하였다.

• 한국산학기술학회논문지
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• 제16권11호
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• pp.7582-7588
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• 2015

광대역 통신을 위한 대표적인 비동기 전송방식인 TR 방식은 동기식 레이크 수신기에 비하여 하드웨어 복잡도가 낮아서 주목을 받아왔다. 최근에는 TR 방식보다 효과적인 데이터 전송을 할 수 있는 BCM 방식이 효율적인 비동기 방식으로 인정받고 있다. BCM 방식의 수신기로는 CMSA 방식이 널리 사용되고 우수한 성능을 가진 것으로 알려져 있다. 본 논문에서는 프레임간 혹은 심볼간 간섭이 존재하는 환경에서, BCM으로 송신된 신호에 대하여 CMSA 방식보다 성능이 우수한 수신기를 제안한다. 제안하는 방식은 TR 방식에서와 같이 상관기를 이용한다. TR방식에서는 하나의 기준신호에 근거한 상관기를 사용하는 반면, 제안하는 방식은 BCM으로 전송된 신호를 복조하기 위하여 블록 코드를 이용한 블록 코드 상관기를 사용한다. 모의실험을 통하여, 제안하는 방식은 CMSA 방식보다 비트오율 측면에서 더 우수한 성능을 나타낸다. 특히, 채널모델 CM4 에서 BER = $10^{-3}$ 일 때, SNR 측면에서 5 dB 이득을 얻을 수 있었다.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.245-255
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• 2021

The spermatozoa become mature in the epididymis which is divided into initial segment and caput, corpus, and cauda epididymis. The water movement across the epididymal epithelium is important for creating luminal microenvironment for sperm maturation. Aquaporins (Aqps) are water channel proteins, and expression of Aqps is regulated by androgens. The current research was focused to examine expressional regulation of Aqp1 and Aqp9 by an androgenic-anabolic steroid, nandrolone decanoate (ND). The ND at the low dose (2 mg/kg body weight/week) or high dose (10 mg) was subcutaneously administrated into male rats for 2 or 12 weeks. Transcript levels of Aqp1 and Aqp9 were determined by quantitative real-time polymerase chain reaction (PCR) analyses. In the initial segment, level of Aqp1 was decreased with 12 week-treatment, while Aqp9 level was decreased by the high dose treatment for 12 weeks. In the caput epididymis, Aqp9 expression was decreased by the low dose treatment. The 2 week-treatment resulted in an increase of Aqp1 level but a decrease of Aqp9 expression in the corpus epididymis. In the corpus epididymis, the 12 week-treatment at the low dose caused the reduction of Aqp1 and Aqp9 levels, but the high dose treatment resulted in an increase of Aqp1 expression and a decrease of Aqp9 level. In the cauda epididymis, Aqp1 expression was decreased by 2 and 12 week-treatments, while increases of Aqp9 levels was detected with the high dose treatment for 2 weeks and with 12 week-treatment. These findings indicate differential regulation of Aqp1 and Aqp9 expression among epididymal segments by ND.