• Title/Summary/Keyword: Differential Coding

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Molecular Cloning of a Gene Encoding a Putative Antibacterial Peptide from Bombyx mori (누에에서 새로운 항세균성 펩타이드 유사 유전자의 분리와 염기서열 결정)

  • 김상현;제연호;윤은영;강석우;김근영;강석권
    • Korean journal of applied entomology
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    • v.35 no.4
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    • pp.321-325
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    • 1996
  • To isolate a novel gene for antibacterial peptide, an inducible clone(BmInc8) was selected by differential screening strategy from Bombyx mori cDNA library prepared from lavae injected with Escherichia coli. This clone contained a cDNA insert of 564 nucleotides and encoded 59 amino acids with an apparent molecular mass of 6.3 kDa. The cDNA sequence of BmInc8 had 61.2% identity compared to that of the bactericidin from Manduca sexta and also the deduced amino acids sequences from this insert had 65% identity compared to that of the cecropin D peptide Hyalophora cecropia. The transient expression assay of this insert using prokaryotic expression vector system revealed that the expressed peptide displayed the antibacterial activity. The cDNA sequence was deposited in GenBank under the accession number U30289.

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A Study on the Robustness of a 16Kbps SBC over the Rayleigh fading Channel Error (16Kbps SBC의 Rayleigh 페이딩 채널에러에 대한 강인성 연구)

  • 오수환;이상욱
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.11 no.4
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    • pp.287-295
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    • 1986
  • In this paper, a SBC(sub-band-coding) is proposed to code a speech signal for a digital mobile radio and a robustness of speech quality of the SBC over the Rayleigh fading channel is investigated via a computer simulation. First the Rayleigh fading channel and 16-ary DPSK receiver models are presentes and verified its validitties by comparing with theoretical values. Three different measures: SNR, LPC distance measure and subjective listening test, were used to evaluate the effects due to the Rayleigh fading channel errors. From the results of computer simulation at BER=$10_{-3}$, $10_{-2}$, 5$ imes$$10_{-2}$, it was found that the speech remained quite intelligible at BER=$10_{-2}$and the link is still usuable even at BER=5$ imes$$10_{-2}$ Thus it was concluded that the SBC can be applicable to the digital mobile radio on the Rayleigh fading channel error in the range of $10_{-4}$~$10_{-2}$ without emplowing any error correction codes.

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Identifying long non-coding RNAs and characterizing their functional roles in swine mammary gland from colostrogenesis to lactogenesis

  • Shi, Lijun;Zhang, Longchao;Wang, Ligang;Liu, Xin;Gao, Hongmei;Hou, Xinhua;Zhao, Fuping;Yan, Hua;Cai, Wentao;Wang, Lixian
    • Animal Bioscience
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    • v.35 no.6
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    • pp.814-825
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    • 2022
  • Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland. Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA). Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day -2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day -14, day -2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved. Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine.

Postprocessing of Inter-Frame Coded Images Based on Convex Projection and Regularization (POCS와 정규화를 기반으로한 프레임간 압출 영사의 후처리)

  • Kim, Seong-Jin;Jeong, Si-Chang;Hwang, In-Gyeong;Baek, Jun-Gi
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.39 no.3
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    • pp.58-65
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    • 2002
  • In order to reduce blocking artifacts in inter-frame coded images, we propose a new image restoration algorithm, which directly processes differential images before reconstruction. We note that blocking artifact in inter-frame coded images is caused by both 8$\times$8 DCT and 16$\times$16 macroblock based motion compensation, while that of intra-coded images is caused by 8$\times$8 DCT only. According to the observation, we Propose a new degradation model for differential images and the corresponding restoration algorithm that utilizes additional constraints and convex sets for discontinuity inside blocks. The proposed restoration algorithm is a modified version of standard regularization that incorporate!; spatially adaptive lowpass filtering with consideration of edge directions by utilizing a part of DCT coefficients. Most of video coding standard adopt a hybrid structure of block-based motion compensation and block discrete cosine transform (BDCT). By this reason, blocking artifacts are occurred on both block boundary and block interior For more complete removal of both kinds of blocking artifacts, the restored differential image must satisfy two constraints, such as, directional discontinuities on block boundary and block interior Those constraints have been used for defining convex sets for restoring differential images.

A Study on Optimal Operation Method of Multiple Microgrid System Considering Line Flow Limits (선로제약을 고려한 복수개의 마이크로그리드 최적운영 기법에 관한 연구)

  • Park, Si-Na;An, Jeong-Yeol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.7
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    • pp.258-264
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    • 2018
  • This paper presents application of a differential search (DS) meta-heuristic optimization algorithm for optimal operation of a micro grid system. The DS algorithm simulates the Brownian-like random-walk movement used by an organism to migrate. The micro grid system consists of a wind turbine, a diesel generator, a fuel cell, and a photovoltaic system. The wind turbine generator is modeled by considering the characteristics of variable output. Optimization is aimed at minimizing the cost function of the system, including fuel costs and maximizing fuel efficiency to generate electric power. The simulation was applied to a micro grid system only. This study applies the DS algorithm with excellence and efficiency in terms of coding simplicity, fast convergence speed, and accuracy in the optimal operation of micro grids based on renewable energy resources, and we compared its optimum value to other algorithms to prove its superiority.

Anti-Jamming Performance of Chaos Communications System Using DCSK and CDSK Modulation Method (DCSK와 CDSK 방식을 사용하는 카오스 통신 시스템의 항재밍 특성)

  • Lee, Jun-Hyun;Ryu, Heung-Gyoon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.24 no.4
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    • pp.417-425
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    • 2013
  • Digital communication technology have been focused on the higher data efficiency and the effective coding of information. Also, it is very important to improve security and minimize the interference of the external sources, Chaos communications system is studied to improve security and minimize the interference by effective encoding of information in the nonlinear systems such as DCSK(Differential Chaos Shift Keying) and CDSK(Correlation Delay Shift Keying). In this paper, we evaluate anti-jamming performance of chaos communications system using the DCSK and CDSK system. The simulation results show that the Gauss iterated map has better BER performance than the other maps(tent map, logistic map, Henon map). Also, DCSK and CDSK system can get the better anti-jamming performance by applying different chaotic maps.

Effects of Launching Vehicle's Velocity on the Performance of FTS Receiver (발사체의 속도가 FTS 수신기의 성능에 미치는 영향)

  • Kang, Sanggee
    • Journal of Satellite, Information and Communications
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    • v.9 no.3
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    • pp.27-32
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    • 2014
  • A doppler shift is generated by moving a transmitter or receiver operated in communication systems. The doppler frequency shift between a transmitter and a receiver or the frequency offset present in transceivers must be removed to get the wanted system performance. FTS is used for preventing an accident from operating abnormally and for guaranteeing public protection. A launching vehicle's initial velocity is very fast in order to escape the earth and the amount of doppler shift is large. Recently many studies to adopt the next generation FTS are ongoing. To introduce new FTS, the effects of doppler shift on the performance of the new FTS must be studied. In this paper the doppler effect caused by launching vehicle's velocity affecting the performance of FTS receiver is investigated into two cases, one is for EFTS as a digital FTS and the other is for FTS using a tone signal. Noncoherent DPSK and noncoherent CPFSK are considered as the modulation methods of EFTS. In the cases of the doppler frequency shift of 200Hz present in EFTS using noncoherent DPSK and noncoherent CPFSK are simulated. Simulation results show that $E_b/N_o$ of 0.5dB deteriorates in the region of near BER of about $10^{-5}$ in RS coding. And there is no performance variation in $E_b/N_o$ or $E_b/N_o$ is worsened about 0.1dB in the same BER region for the case of using convolutional and BCH coding. Quadrature detector used in FTS using tone signals is not influenced by the doppler frequency shift.

No-Referenced Video-Quality Assessment for H.264 SVC with Packet Loss (패킷 손실시 H.264 SVC의 무기준법 영상 화질 평가 방법)

  • Kim, Hyun-Tae;Kim, Yo-Han;Shin, Ji-Tae;Won, Seok-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.11C
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    • pp.655-661
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    • 2011
  • The transmission issues for the scalable video coding extension of H.264/AVC (H.264 SVC) video has been widely studied. In this paper, we propose an objective video-quality assessment metric based on no-reference for H.264 SVC using scalability information. The proposed metric estimate the perceptual video-quality reflecting error conditions with the consideration of the motion vectors, error propagation patterns with the hierarchical prediction structure, quantization parameters, and number of frame which damaged by packet loss. The proposed metric reflects the human perceptual quality of video and we evaluate the performance of proposed metric by using correlation relationship between differential mean opinion score (DMOS) as a subjective quality and proposed one.

RNase P-dependent Cleavage of Polycistronic mRNAs within Their Downstream Coding Regions in Escherichia coli

  • Lee, Jung-Min;Kim, Yool;Hong, Soon-Kang;Lee, Young-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.29 no.6
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    • pp.1137-1140
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    • 2008
  • M1 RNA, the catalytic subunit of Escherichia coli RNase P, is an essential ribozyme that processes the 5' leader sequence of tRNA precursors (ptRNAs). Using KS2003, an E. coli strain generating only low levels of M1 RNA, which showed growth defects, we examined whether M1 RNA is involved in polycistronic mRNA processing or degradation. Microarray analysis of total RNA from KS2003 revealed six polycistronic operon mRNAs (acpP-fabF, cysDNC, flgAMN, lepAB, phoPQ, and puuCBE) showing large differences in expression between the adjacent genes in the same mRNA transcript compared with the KS2001 wild type strain. Model substrates spanning an adjacent pair of genes for each polycistronic mRNA were tested for RNase P cleavage in vitro. Five model RNAs (cysNC, flgMN, lepAB, phoPQ, and puuBE) were cleaved by RNase P holoenzyme but not by M1 RNA alone. However, the cleavages occurred at non-ptRNA-like cleavage sites, with much less efficiency than the cleavage of ptRNA. Since cleavage products generated by RNase P from a polycistronic mRNA can have different in vivo stabilities, our results suggest that RNase P cleavage may lead to differential expression of each cistron.

Disruptions of Two Apparent rho-Independent Transcription Terminator Structures do not help in Enhancing the Expression of aceK in E. coli

  • Lee, Su-Ji;Chung, Taeo-Wan
    • BMB Reports
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    • v.28 no.5
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    • pp.458-463
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    • 1995
  • Two apparent rho-independent transcription terminator structures within the coding sequence of aceK have been destroyed to access their roles in the differential expression between aceA and aceK in the glyoxylate bypass operon of E. coli. The effect of mutations on the expression of aceK was evaluated in two different ways: one by maxicell labeling and the other by lacZ fusion gene construction. The maxicell labeling experiment with the mutant operon clones has failed, like that of the wild type operon clone, to visibly show isocitrate dehrogenase (IDH) kinase/phosphatase, the product of aceK, on the autoradiogram of a protein gel. When the same mutations were introduced into an aceK::lacZ fusion gene to quantitatively evaluate the mutational effect, the activity of ${\beta}-galactosidase$ in neither of the mutant versions of the fusion gene was elevated significantly enough to explain the degree of polarity observed in this region. Thus, we conclude that neither of these intragenic, apparent rho-independent transcription terminator structures, which have long been suspected as a major determinant in the down regulation of aceK, really act as a premature transcriptional terminator.

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